direct electrical stimulation of myogenic cultures for analysis of muscle fiber type control
Báo cáo khoa học: Death-associated protein kinase (DAPK) and signal transduction: fine-tuning of autophagy in Caenorhabditis elegans homeostasis doc
Ngày tải lên :
29/03/2014, 08:20
... is a ceiling effect of basal levels of autophagy under normal conditions It will be interesting to test whether a gain-offunction mutation of tax-6 can decrease high levels of autophagy under ... responsible for the activation of S6K during starvation, which is required for the induction of autophagy Three recent papers support this hypothesis: previous studies have shown that some level of S6K ... review, we emphasize the role of DAPK in the regulation of autophagy Although several aspects of autophagy regulation have been discovered, further examination of the various regulatory pathways...
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Báo cáo sinh học: "Regulation of metabolism in Caenorhabditis elegans longevity" ppsx
Ngày tải lên :
06/08/2014, 19:21
... downregulation of TOR Mutation of DAF-15/RAPTOR, an activator of TOR, results in increased C elegans life span [13] Interestingly, daf-15 is directly regulated by DAF-16, the ultimate effector of the ... studied in the metabolomic analysis performed by Fuchs et al [7] It was previously observed that knockdown of TOR/let-363 pathway in C. elegans results in an almost twofold increase in life span ... effects of downregulating protein synthesis might be specific to C elegans and other invertebrates The soma of the adult nematode is postmitotic, and metabolic control might have different Page of...
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Báo cáo sinh học: "Robust and specific inhibition of microRNAs in Caenorhabditis elegans" doc
Ngày tải lên :
06/08/2014, 19:21
... and lack of vulva formation, known phenotypes for lin-4(lf ) [3] The progeny of worms injected with antisense oligonucleotides complementary to let-7 demonstrated a bursting vulva phenotype with ... respective loss of function mutant strains of lin-4 and let-7 For example, progeny of animals injected with antisense oligonucleotides against lin-4 show delayed exit in differentiation of seam cells ... phenotype with lack of alae (longitudinal ridge) formation in 100% of animals, consistent with the phenotype shown by let-7 knockout mutant animals [5] By contrast, progeny of animals treated...
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Báo cáo khoa học: Functional analysis of pyrimidine biosynthesis enzymes using the anticancer drug 5-fluorouracil in Caenorhabditis elegans docx
Ngày tải lên :
30/03/2014, 01:20
... mutant phenotype analysis Analysis of 5-FU sensitivity was performed on plates containing 5-FU (800 nm) Synchronized embryos were transferred to 5-FU plates After 60 or 72 h, the numbers of L4 larvae ... sheets with lL of nonradioactive tracer (100 lg of 5-FU and 100 lg of uridine for the UP assay mixture; 100 lg of 5-FU and 100 lg of deoxyuridine for the TP assay mixture) After development with ... presence of uracil and thymine, respectively The enzymatic activities of three mutant UPP-1 proteins were very low as compared with that of wild -type UPP-1 in vitro The TP activity of UPP-1 is...
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Báo cáo khoa học: Stage-specific activation of MIG-17⁄ADAMTS controls cell migration in Caenorhabditis elegans docx
Ngày tải lên :
30/03/2014, 04:20
... activated form, was immunoblotted (right lane) Single asterisk, proform of MIG17–GFP; double asterisk, activated form of MIG-17–GFP; arrow, proform of MIG-17– 6His; arrowhead, activated form of MIG-17– ... in both the proform and activated form in the recombinant enzyme after its secretion into the culture medium (Fig 2B) To determine the N-terminal sequence of the activated form of MIG-17–6His, ... raised using the polypeptide of the MIG-17 activated form expressed in Escherichia coli (Fig 2C) The polyclonal antibody recognized both the proform and activated form of MIG-17–GFP expressed in...
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Báo cáo sinh học: "A global analysis of genetic interactions in Caenorhabditis elegans" doc
Ngày tải lên :
06/08/2014, 18:21
... number of bridges as the number of possible (tested) gene pairs between the subnetworks times the probability of linking a gene pair for that data type An estimate of the probability of a data type ... boost is larger for more infrequent phenotypes For example, a phenotype that occurs in out of 100 genes will increase the score by units, whereas a phenotype that occurs in out of 10 genes will ... separate data types Determination of the significance of the number of supported links The significance of the number of supported links (gene pairs linked by more than one data type) in the superimposed...
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Báo cáo y học: "Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails" ppsx
Ngày tải lên :
14/08/2014, 14:21
... is used for microarray analysis culture of their embryonic precursors followed by selection of the cells by flow cytometry [18] Although embryonic cell cultures allow the collection of cells ... of neurons in C elegans, profiling of the gene-expression pattern of each type of neuron should be possible with this technique interactions The expression patterns of the genes indicated in bold ... disadvantage of using a cDNA microarray Acquisition of cDNA clones for rare mRNA species and use of wholegenome microarrays are complementary approaches for improving the applicability of the method...
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Báo cáo y học: "Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution" pdf
Ngày tải lên :
14/08/2014, 17:22
... the strength of RNAi phenotypes for many genes was indeed reduced with increasing dilution of control bacteria (Figure 3) For example, we were able to detect phenotypes for about 90% of genes with ... both genes of a duplicate pair Quantitative analysis of page) Quantitative analysis of synthetic phenotypes following the simultaneous targeting of both genes of a duplicate pair For duplicate ... variations in the activities of other genes will affect this phenotype (for review [4]) If changes in the activity of one gene affect the loss -of- function phenotype of a second gene, then these...
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Báo cáo y học: "Identification of ciliary and ciliopathy genes in Caenorhabditis elegans through comparative genomics" pptx
Ngày tải lên :
14/08/2014, 17:22
... chain, isoform Many, most, all ciliated neurons [16,65] Phospholipase D3, isoform Pharynx, head neurons + Bardet-Biedl syndrome protein Many, most, all ciliated neurons [10,16] Splice isoform of intraflagellar ... upstream of the start codon Applying a more stringent criterion of X-boxes within 250 bp upstream of the start codon, 293 genes were uncovered Blacque et al also performed serial analysis of gene ... of SAGE datasets that were previously generated by the C elegans Gene Expression Consortium [33] for various tissue types, including the ciliated cell subset of neuronal cells [17] For each type...
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Báo cáo khoa học: Cyclic ADP-ribose requires CD38 to regulate the release of ATP in visceral smooth muscle ppt
Ngày tải lên :
05/03/2014, 23:20
... of ATP released in the bladder In particular, we studied the effects of exogenous cADPR on spontaneous and electrical field stimulation (EFS)-evoked overflow of ATP in bladder detrusor smooth muscle ... peaks of b-NAD+ + ADPR + cADPR, AMP and Ado represented the amounts of endogenously formed nucleotides and nucleosides plus products of the degradation of the exogenous cADPR, and therefore were ... examined the effect of carbachol, a stable analog of acetylcholine, on the spontaneous overflow of ATP Carbachol (1 lm) caused no additional formation of ATP in bladder detrusor muscles isolated...
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Báo cáo Y học: Dystrobrevin requires a dystrophin-binding domain to function in Caenorhabditis elegans doc
Ngày tải lên :
08/03/2014, 23:20
... protein and the various forms of the AD-Dyb-1 fusion proteins were analysed on the basis of transactivation of the HIS3 reporter gene after days of growth on SD medium devoid of tryptophan, leucin ... incubated for h at °C GST controls were performed using 1–2 times the amount of fusion protein After five washes with binding buffer, the labelled proteins were eluted by boiling the preparation for ... we tested the ability of the various forms of Dyb-1 to interact with Dys-1 in a two-hybrid assay The Dyb-1 control and mutant clones were fused to the activation domain of the Gal4 yeast transcription...
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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
Ngày tải lên :
16/03/2014, 00:20
... (µM) % of lactamase release B 100 80 60 40 20 10 32 15 20 Time (min) Fig Permeation of E coli OM by Esc(1–18) (A) Effects of different concentrations of Esc(1–18) on permeation of the OM of E coli ... Permeation of the bacterial IM To assess the ability of Esc(1–18) to alter the permeability of the IM of E coli, · 106 cells were mixed with lm SYTOX Green in NaCl ⁄ Pi for in the dark After addition of ... with 32 m Esc(1–18) for and 20 min, and then centrifuged at 300 g for 20 Controls were performed in the presence of peptide solvent (20% ethanol), at a final concentration of 0.6% The pellets...
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Báo cáo khoa học: Lysosomal localization of GLUT8 in the testis – the EXXXLL motif of GLUT8 is sufficient for its intracellular sorting via AP1- and AP2-mediated interaction docx
Ngày tải lên :
16/03/2014, 02:20
... blot analysis of total membranes from testis of wild -type and GLUT8 knockout mice Total membranes were prepared as previously described [4] Western blot analysis was performed with 20 lg of total ... specifically controls localization and sorting of both transporters [41] The absence of the GLUT8–TAC chimera at the plasma membrane indicated that a majority of the chimeric protein is directly ... physiological role of the evolutionarily ‘oldest’ class III GLUT family isoforms is not understood – especially in the context of their intracellular localization as described for all class III...
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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
Ngày tải lên :
16/03/2014, 02:20
... performed in the presence of 0.25 mm dNTPs, 1.5 mm MgCl2, 10 pmol of each primer and 0.5 U Taq polymerase, and consisted of 35 cycles of denaturation at 94 °C for 45 s, annealing at 58 °C for ... absence of any of these were taken as the control (Con) On termination of incubation, cells were lysed and the nuclear (50 lg) and cytosolic (40 lg) protein extracts were assessed for the presence of ... constitutively active form of Akt demonstrated restoration of this preventative effect of TNFa on insulin action [30] As our study was directed towards the underlying mechanisms of insulin and TNFa...
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Báo cáo khoa học: Glutathione transferases kappa 1 and kappa 2 localize in peroxisomes and mitochondria, respectively, and are involved in lipid metabolism and respiration in Caenorhabditis elegans pot
Ngày tải lên :
16/03/2014, 03:20
... 0.25 mL of NaOH (0.1 m) overnight for protein measurement using the Bio-Rad assay For each analysis, lipids from the homogenate were extracted according to Bligh and Dyer [37] in chloroform–methanol–water ... internal standard For free fatty acid analysis, 400 lL of homogenate were extracted and dissolved in mL of hexane Free fatty acids were transmethylated in mL of BF3 ⁄ CH3OH (10% w ⁄ w) for at room ... S1D), indicating the specificity of these RNAi forms and the absence of compensatory adaptation [i.e upregulation of gstk-1 in gstk-2(RNAi) worms] RNAi silencing of gstk-1 or gstk-2 had no apparent...
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Báo cáo khoa học: The astacin protein family in Caenorhabditis elegans docx
Ngày tải lên :
23/03/2014, 15:21
... to the PCR reaction The cycling conditions were 94 °C for min, 94 °C for 40 s, 55 °C for 40 s, 68 °C for per kb for 35 cycles, and 68 °C for After PCR, samples were analyzed in 2% agarose gels ... deletions or frame shifts (EMBL: ALIGN_000543) For identification and annotation of protein domains and the analysis of domain architectures the tools of the SMART [33], PFAM [26], ProDom [34] and ... phylogenetic method For neighbor-joining analysis the PHYLIP 3.5 software package [37] was used Distances between the pairs of protein sequences were calculated and corrected for multiple changes...
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Báo cáo khoa học: Identification and localization of glycine in the inner core lipopolysaccharide of Neisseria meningitidis ppt
Ngày tải lên :
23/03/2014, 21:21
... hydrolysis of immunotype L3 galE and L4 galE LPS afforded core oligosaccharides that were initially examined by ES-MS (Fig 1) Several ions were observed and the compositions for each glycoform are ... 31P-resonance of the PEtn molecule at the 6-position of HepII to 1H-resonances at 4.58 p.p.m which is characteristic for substitution of the 6-position of Table 1H NMR assignment of HepII residue ... observed for the -CH2- protons of the glycine moiety in meningococcal LPS points to an exocyclic location for the glycine residue in N meningitidis LPS The genetic control of the elaboration of glycine...
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A pathway that links reproductive status to lifespan in Caenorhabditis elegans pptx
Ngày tải lên :
28/03/2014, 16:20
... about the site of action of DAF-12.) Moreover, TCER-1 is required for the increased expression of a set of DAF-16regulated genes in response to germline loss, but TCER-1 is not required for lifespan ... prevent loss of the germline from extending lifespan.13,16,17 (For a summary of genes in this pathway, see Table 1.) DAF-12/NHR is not the only transcription factor required for loss of the germ ... required for loss of the germline to extend the lifespan of C elegans For references, see text Gene General function Response to germline loss Role in insulin/IGF-1 pathway Required for insulin/IGF-1-pathway...
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Báo cáo sinh học: "The short coiled-coil domain-containing protein UNC-69 cooperates with UNC-76 to regulate axonal outgrowth and normal presynaptic organization in Caenorhabditis elegans" ppt
Ngày tải lên :
06/08/2014, 18:21
... [27], 47% of unc-76(e911) mutants (n = 101) had the AWCOFF phenotype; e911 was the strongest allele among all the nine alleles that we tested For the other unc-76 alleles, the AWCOFF phenotype varied ... function of SCOCO with in ovo RNA interference (RNAi) [45] resulted in aberrant pathfinding of the epaxial nerve fibers (Figure 5n) The epaxial nerve is formed by axons of motoneurons of the MMC ... regions of UNC-69, UNC-76 and their homologs were assigned on the basis of a greater than 80% probability of forming coiled coils according to this program We used ClustalW or T-Coffee for multiple...
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