digestion of extracellular rna
Continuous Anaerobic Digestion of Food Waste and Paper Waste under Mesophilic-Dry Condition
... anaerobic digestion of the organic fraction of municipal solid waste: focusing on the start-up phase, Bioresource Technol., 86, 123-129 Fernandez J., Perez M and Romero L I (2008) Effect of substrate ... landfill, conservation of resources, and reduction of CO2 emissions, dry digestion is gaining much attention However, most researches have been limited to batch tests or investigation of the start-up ... treatment of organic solid waste is an urgent issue In the present work, the performance of a continuous dry anaerobic digestion process under mesophilic condition was investigated A mixture of food...
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Tài liệu Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses pdf
... mechanism of psychrotrophic RNase HI M S Rohman et al changed regardless of whether they are introduced into So-RNase HI or 4·-RNase HI The stabilities of the 4·-RNase HI, 5·-RNase HI and 6·-RNase ... denaturation of So-RNase HI and its derivativesa Protein Cm a (M) Ma (kJÆmol)1ÆM)1) DG (H2O)a (kJÆmol)1) DDG (H2O)a (kJÆmol)1) So-RNase HI R97G-RNase HI D136H-RNase HI 4·-RNase HI 5·-RNase HI 6·-RNase ... specific activities of the 5·-RNase HI and 6·-RNase HI proteins were 65% and 43%, respectively, of that of the wild-type protein, and 93% and 66%, respectively, of that of the 4·-RNase HI protein...
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Tài liệu Báo cáo khoa học: Rac upregulates tissue inhibitor of metalloproteinase-1 expression by redox-dependent activation of extracellular signal-regulated kinase signaling pptx
... sustained activation of Rac by overexpression of the Rac-specific activator, Tiam1, or overexpression of V12-Rac1, strongly induces transcriptional upregulation of tissue inhibitor of metalloproteinase ... four different isoforms Of these, the prototype member of the TIMP family, TIMP-1, is of particular interest, because in addition to its classic role as a broad specific inhibitor of active matrix ... well-established roles of (a) AP-1 in regulating the transcription of numerous genes, and (b) of TIMP-1 as an inhibitor of tumor invasion and metastasis as well as a regulator of cell proliferation...
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Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx
... (lgÆmL)1) RNase A RNase-AA RNase-AA-G RNase-AA-GG HP-RNase HHP-RNase BS-RNase Bovine pancreatic RNase A A19P,Q28L,K31C,S32C-RNase A D38G-RNase-AA D38G,E111G-RNase-AA Human pancreatic RNase Q28L,R31C,R32C,N34K-HP-RNase ... Aggregation of DMPG vesicles induced by RNases Symbols: (e) BS-RNase; (+) RNase AA-GG; (n) HHP-RNase; (n) RNase AA-G; (h) RNase AA; (m) HP-RNase; (·) RNase A Electrostatic interactions and antitumor RNases ... function of protein : DMPG ratio Fig Effect of RNases on the thermotropic behavior of DMPG vesicles Symbols: (e) BS-RNase; (+) RNase AA-GG; (n) HHP-RNase; (n) HP-RNase; (h) RNase AA-G; (m) RNase...
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Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf
... pattern of the 12-bp RNA DNA hybrid were similar to those of archaeal Type RNase H [19,20] Archaeal Type RNase H can cleave an RNA DNA junction (a junction between the 3¢ side of RNA and 5¢ side of ... example of a genome with three active RNase H genes FEBS Journal 272 (2005) 2828–2837 ª 2005 FEBS A fusion protein consisting of RNase H and APase complementation of MIC2067(DE3) 159 497 290 + + RNase ... domain of SCO2299 could cleave the RNA strand of the RNA DNA hybrid but the C-terminal region could not This result agreed with that of the in vivo complementation assay The cleavage efficiency of...
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Tài liệu Báo cáo khoa học: A role for the intersubunit disulfides of seminal RNase in the mechanism of its antitumor action docx
... FEBS 2003 Disuldes and antitumor action of BS-RNase (Eur J Biochem 270) 1983 Fig Time-course of dimerization of the labelled monomeric derivative of BS-RNase 125I-labelled MSSAE added to growing ... aminoethanethiosulfonate of the sulfhydryls exposed by selective reduction of the Table Monomeric derivatives of BS-RNase LC50 is the protein concentration producing 50% of cell death Mixed LC50 ... cells of monomeric derivatives of BS-RNase Cells were treated for 48 h at 37 C with MCA (h), MCM (j), MAE (e), MSSAE (r), MSSG (m) or BS-RNase as a positive control (d) 1ị 2ị where M is a BS-RNase...
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Báo cáo khoa học: Catalytic digestion of human tumor necrosis factor-a by antibody heavy chain pot
... conformation of ETNF-6 mAb Sequencing of the cDNA of the variable region of ETNF-6 mAb was performed, and this was followed by molecular modeling of its three-dimensional structure The heavy chain of ETNF-6 ... a FEBS Journal 277 (2010) 3823–3832 ª 2010 The Authors Journal compilation ª 2010 FEBS E Hifumi et al Catalytic digestion of TNF-a by the antibody subunit Fig Nucleotide sequences of cDNA and ... chromatograms After a reaction time of 47.2 h, the amount of TP41-1 at a retention time of 11 decreased, because of fragmentation The fragment was observed at a retention time of 13 (indicated by an arrow)...
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
... results offer clues for comparison of the residues involved in the specificity of RNA cleavage within the toxin family and for the design of RNases based on the different cleavage efficiencies of Kid ... contribution of the T46 residue to RNA cleavage These results also show a good correlation between RNase activity, protein synthesis inhibition and in vivo inhibition of Analysis of Kid RNase model ... activity of this mutant (Fig 2) Further analysis of this activity on longer RNA substrates (CopT or CopA, which are RNA regulatory elements of R1 plasmid replication, and TAR, a regulatory region of...
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Báo cáo khoa học: RNA-binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA pdf
... 0.0067% of Triton X-100 and 67 lgÆmL)1 of yeast tRNA (Sigma) for 15 The protein and RNA were crosslinked by 1.8 J of UV irradiation in a UV-crosslinker (Hoefer Inc.) following digestion of the RNA ... flow rate of 0.5 mLÆmin)1 Proteins were precipitated by cold acetone and analyzed by SDS/PAGE For digestion of the RNA, the extract was incubated with RNase A (1 mgÆmL)1) and UÆlL)1 of RNase T1 ... to RNA processing [20] Function of HCF152 in petB RNA maturation Fig RNA affinities of truncated proteins containing different numbers of PPR motifs (A) RNA- binding of the HCF152 and truncated parts...
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Báo cáo khoa học: Suppression of urokinase receptor expression by bikunin is associated with inhibition of upstream targets of extracellular signal-regulated kinase-dependent cascade pptx
... suppression of cell-associated plasmin activity and expression of uPA mRNA and protein However, little is known concerning the potential role of bikunin in the regulation of uPAR mRNA and its ... in an increase in the number of binding sites and a decrease of the affinity of the uPAR [38,39] The quantification of uPAR expression by measurement of the amount of uPA bound is thus not ideal ... suppression of uPAR expression (not shown) The reduced amount of uPAR mRNA reflected a diminished amount of uPAR protein as determined by the [125I]DIP-uPA binding assay and ELISA Fig Levels of uPAR mRNA...
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Báo cáo Y học: Effect of the disease-causing mutations identified in human ribonuclease (RNase) H2 on the activities and stabilities of yeast RNase H2 and archaeal RNase HII pot
... ng of Sc-RNase H2*; lane 3, 100 ng of Sc-RNase H2*; lane 4, 18 pg of Tk-RNase HII; lane 5, 180 pg of Tk-RNase HII The sequences of DNA1 5RNA1 -DNA13 of [rA]1, DNA13 -RNA4 -DNA12 of [rA]4, and RNA2 9 ... of Sc-RNase H2* to substrate is comparable to that of Tk-RNase HII, whereas the turnover number of Sc-RNase H2* is much lower than that of Tk-RNase HII Construction of mutant proteins of Sc-RNase ... Alignment of the amino acid sequences of Tk-RNase HII (Tko), Sc-Rnh2Ap (Sce), and Hs-RNASEH2A (Hsa) (B) Alignment of the amino acid sequences of Sc-Rnh2Bp (Sce) and Hs-RNASEH2B (Hsa) (C) Alignment of...
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Báo cáo khoa học: Comparison of human RNase 3 and RNase 7 bactericidal action at the Gram-negative and Gram-positive bacterial cell wall pot
... displacement assay was performed by the addition of 1–2 lL aliquots of a solution of Polymyxin B, RNase 3, RNase or RNase A to mL of a continuously stirred mixture of LPS (10 lgÆmL)1) and Bodipy TR cadaverine ... Comparison of the membrane interaction mechanism of two antimicrobial RNases: RNase ⁄ ECP and RNase Biochim Biophys Acta 1788, 1116–1125 11 Pizzo E & D’Alessio G (2007) The success of the RNase scaffold ... estimated value of 1.5 lm for RNase activity with E coli cells, whereas no agglutination FEBS Journal 277 (2010) 1713–1725 ª 2010 The Authors Journal compilation ª 2010 FEBS 1715 RNase and RNase bactericidal...
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Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx
... analysis of the Cc RNase Results Expression analysis of Cc RNase In our previous work, we reported the cloning and overexpression of a cDNA sequence of 864 bp (Cc RNase cDNA) that encodes a specific RNase ... processing of micro RNAs (miRNAs), and play key roles in miRNA-mediated gene regulation of processes such as development and differentiation [9,10] In Bombyx mori, a homolog of the RNase L inhibitor ... acceptor [19] Alternative polyadenylation of the Cc RNase gene generates two mRNA isoforms The finding that no additional intronic sequence is present in the 3¢ UTR of the Cc RNase gene indicates...
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Báo cáo Y học: Mammalian mitochondrial endonuclease G Digestion of R-loops and localization in intermembrane space doc
... the other hand, RNase MRP has been shown to cleave RNA of triplestranded R-loops preferentially at the CSBs [5], and is another RNase proposed to create RNA primers The role of endoG in mtDNA ... was never observed during endoG treatment of R-loops over the time course of the study (Fig lower panel) If RNA was first cleaved with RNase H activity of endoG, the R-loops would have reverted ... mtDNA replication by cleaving the RNA moiety of RNA DNA hybrids formed at the origin of replication [3] However, based on our observations, endoG would not act as an RNase in these conditions, but...
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Báo cáo khoa học: Analysis of the RNA degradosome complex in Vibrio angustum S14 potx
... [11,20,21] The molecular interactions of RNase E with other proteins of the RNA degradosome are proposed to be mediated by short microdomains of 15–40 amino acids on RNase E [22] These microdomains ... when subjected to digestion by trypsin This may have occluded the identification of RNase E in the mass spectrometric analysis of Bands A and B However, RNase E was detected by MS of immunoprecipitates ... fragment of RNase E containing its CTH This would be consistent with the known positions of the microdomains in RNase E Although more unlikely, we cannot rule out that, if this fragment of RNase...
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Báo cáo khoa học: Role of extracellular signal regulated kinases 1 and 2 in neuronal survival docx
... Ó FEBS 2004 Role of ERK1/2 in neuronal survival (Eur J Biochem 271) 2051 overexpression of mutated elements of the pathway, use of pharmacological inhibitors and genetic ... synaptosomes [8] The interpretation of the data obtained through experimental modulation of ERK1/2 signaling may be hampered by the fact that inhibition of one element of the ERK1/2 signaling network ... activation of ERK1/2 reached levels similar to those observed at the peak of BDNF action [16] Inhibition of ERK1/2 by either pharmacological inhibitors or by a dominant-negative mutant form of MKK1...
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Báo cáo khoa học: Mode of action of the microbial metabolite GE23077, a novel potent and selective inhibitor of bacterial RNA polymerase docx
... might inhibit RNA synthesis by preventing RNAP from binding to DNA Binding of RNAP to DNA is indeed one of the earliest steps of the transcription process and a possible molecular target of a transcription ... totally inhibited RNA synthesis when added before DNA, whereas Fig Effect of GE23077 and other RNA polymerase (RNAP) inhibitors on in vitro RNA synthesis: comparison of the effects of compound addition ... gift of A Galizzi (Institute of Genetics, University of Pavia, Italy) [20] Rifampicinresistant (rifR) E coli RNAP (rpoB3) was from Promega (Madison, WI, USA); rifR RNAP (rpoB7) and rifR RNAP...
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Báo cáo Y học: Optimizing the delivery systems of chimeric RNA . DNA oligonucleotides Beyond general oligonucleotide transfer ppt
... Correction of the mutation responsible for sickle cell anemia by an RNA- DNA oligonucleotide Science 273, 1386–1389 12 Wu, X.S., Liu, D.P & Liang, C.C (2001) Prospects of chimeric RNA- DNA oligonucleotides ... results showed that RDO converted AfiC at Ser365 of factor IX gene in rat liver specifically Factor IX coagulant activities of rats Ó FEBS 2002 Optimizing RNA DNA oligonucleotide delivery systems (Eur ... than PEI [34] Dendrimers are a new kind of reproducible substances, with a hydrocarbon core and charged surface of amino groups They have the advantage of having a defined small size However, their...
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mesophilic and thermophilic anaerobic digestion of nsource-sorted organic wastes _ effects of amm
... gram of COD or per gram of volatile solids degraded was high in both of our reactors This was the result of a high lipid and fat content of the biowaste input material, caused by the addition of ... respectively, after sedimentation of large solids Whereas the supernatant of the mesophilic reactor euent contained mainly soluble organic components, the supernatant of the thermophilic reactor euent ... Mackie and Bryant (1995) for the digestion of cattle manure The advantage of thermophilic digestion was mainly that the euent was rendered hygienic by inactivation of bacteria (Kubler 1994) or viruses...
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báo cáo hóa học:" Gene and microRNA analysis of neutrophils from patients with polycythemia vera and essential thrombocytosis: down-regulation of micro RNA-1 and -133a" pot
... the expression of miR-216 and miR-217 and the lack of expression of miR-133a were characteristic of pancreatic tissue [36] Page of 17 (page number not for citation purposes) Journal of Translational ... http://linus.nci.nih.gov/BRBArrayTools.html MicroRNAs Expression Profiling A microRNA probe set was designed using mature antisense microRNA sequences (Sanger data base, version 9.1) consisting of 827 unique microRNAs from human, ... healthy subjects The results of analysis by qRT-PCR and microRNA expression profiling were similar Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine 2009,...
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