differentiation of rhesus monkey embryonic stem cells in three dimensional collagen matrix pdf
Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... apolipoprotein A-I apolipoprotein A-II apolipoprotein B apolipoprotein C-I apolipoprotein C-II apolipoprotein D apolipoprotein E apolipoprotein F apolipoprotein H fatty acid binding protein 1, liver ... 43 °C Finally, the chip was rinsed in 0.05· SSC before drying by low-speed centrifugation For staining, the chip was immersed in an NaCl ⁄ Pi solution containing 10 lgÆmL)1 of streptavidin ⁄ R-phycoerythrin ... Transcriptome in hepatic induction of AT-MSCs analyzed using genmapp software version 2.1 and visualized using ConPath Navigator, a tool that enables viewing and searching of results obtained by genmapp...
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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx
... droplets (Fig 1B) The colonies of ASCs were subjected to Alizarin red S staining weeks after the initiation of osteogenic differentiation Intense Alizarin red S staining of the colonies confirmed ... H3: Immunofluorescence staining Scale bars = 50 μm Transplantation of ASCs in spinal cord injury 281 Fig Immunofluorescence staining of ASC group; A1-A3: Glial fibrillary acidic protein (GFAP), ... cells For group V, the injured site was exposed by dorsal laminectomy and 150 μL of PBS was injected into the spinal cord at locations to depths of mm using a 30 gauge needle (middle of the injury...
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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt
... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... totipotent embryonic stem cells cultured in three- dimensional micromass technique in the absence of any scaffold The osteogenic differentiation of the cells was induced by the addition of dexamethasone, ... identifying substances that enhance mineralization The use of embryonic stem cells will probably play a major role in tissue engineering in the future because of the remakable potential and differentiation...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics ... Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and sanctity of human life The ... destruction of additional human embryos Indeed, the embryos from which the existing ESCs originated have already been destroyed Federal funding of medical research on these existing stem cell lines...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific miRNA is a major obstacle in understanding the miRNA ... expression profiling of single whole embryonic stem cells Nucleic Acids Res 2006, 34(2):e9 Chen C, Ridzon D, Lee CT, Blake J, Sun Y, Strauss WM: Defining embryonic stem cell identity using differentiation- related ... expression profiles of miRNAs and transcripts using three hES cell lines, embryonic bodies (EB) produced from one of the cell lines and five types of terminally differentiated adult cells The integration...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... could maintain a small accessible region in the globin locus during the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this ... recruitment at the murine b-globin locus in uninduced embryonic stem cells (day 0), as well as that of primitive and definitive erythroid cells (days and 12, respectively) Using chromatin immunoprecipitation ... marks to the b-globin gene locus during erythroid differentiation of murine ES cells in vitro In these experiments we utilized the ES ⁄ OP9 cell in vitro differentiation system described by Kitayima...
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differentiation of embryonic stem cells
... of Megakaryocytes from Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation of Embryonic ... YAMAZAKI, AND SHIN-ICHI HAYASHI 98 v vi TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils ... Mutagenesis in Mouse Embryonic Stem Cells YIJING CHEN, JAY L VIVIAN, AND TERRY MAGNUSON 406 Section IV Differentiation of Monkey and Human Embryonic Stem Cells 29 Growth and Differentiation Monkey ES Cells...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... ES cells We induced differentiation of human ES cells in an in vitro two -dimensional culture on OP9 stromal cell line and examined the expression of VEGF-R2, VE-cadherin and TRA-1 during the differentiation ... purposes) Journal of Translational Medicine 2008, 6:54 cells, the number (cells/ mm2/mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected ... the saline- or hMNCs-injected groups, CBF in the hES-ECinjected group increased significantly, while no significant increase in the number of mouse PECAM-1 positive cells was observed in the...
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Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps
... fixed in 1% Formaldhyde in PBS for 15 minutes at room temperature Cells were rinsed with PBS twice and incubated in a 1/10000 dilution solution of DAPI (10 mg/ml) in PBS for 15-20 minutes Cells ... library in Genbank (Genbank ID: BG970953.1) with the same intron splicing pattern as the truncated form of MsrA cloned from embryonic stem cells, indicating this isoform might not be stem cell ... clearer three dimensional configuration of the subcellular localization of the truncated protein than a single view On the focal point of the scans (crosspoint of the horizontal green line and...
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báo cáo khoa học:" Induction of osteogenic markers in differentially treated cultures of embryonic stem cells" ppt
... xenogenic cells [13-16] There are some reports that use totipotential embryonic stem cells in tissue engineering of bone [17,18] Embryonic stem cells (ESCs) are routinely derived from the inner cell ... are von Kossa stainings with arrows pointing to the deposition of calcium phosphate-containing minerals that stained in black Figure LIF, stem cell PCR showing amplification of the without hematopoeticqualitative ... adipogenesis in embryonic stem cells by bone morphogenetic protein-2: effect of cofactors on differentiating lineages BMC Dev Biol 2005, 5(1):1 Bielby RC, Boccaccini AR, Polak JM, Buttery LD: In vitro differentiation...
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Báo cáo y học: " Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment" pdf
... inhibition of IκB kinase Page of 15 [13,18] There is increasing interest in curcumin as a therapeutic option for OA and RA, with evidence that curcumin inhibits the IL-1β-induced activation of ... curcumin pre-treatment did not lead to production of CSPGs, collagen type II, β1-integrin and activation of Shc, ERK 1/2 and Sox-9 In contrast, production of CSPGs, collagen type II and β1-integrin ... mechanism of action of curcumin in MSCs and chondrocytes might involve the β1-integrin receptor signalling pathway Several studies have suggested that curcumin interacts with the TGF-β signaling cascade,...
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Báo cáo y học: "The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells" pot
... fibroblasts remained after 20-25 minutes plating in non-gelatinized plates Pre-plating of feeder-dependent ES cells in this way may result in a slight delay in the apparent time of replication ... replication in ES cells is altered by mutation of chromatin modifiers Satellite Satellite replication in ES cells is altered by mutation of chromatin modifiers (a) Summary of replication timing of repeat ... Poole, UK) of the D3 clone Deletion of both alleles was confirmed by genotyping The ES cell lines were maintained in the undifferentiated state by culturing on gelatinized plates in KO-DMEM (Invitrogen,...
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Báo cáo y học: "A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells" doc
... ES cells after a single round of infection with 0.1 ml viral supernatant are illustrated by staining puromycin-resistant colonies in 24-well plates Figure (see following page) Super-infection of ... receptor of MuLV in fibroblasts [16] The proviral-host junction sequences mapped to five different positions in introns and of the mCat-1 gene, demonstrating the independent origin of each of these ... cell in the culture has at least one infection event In practice, the need to infect every cell in a culture of 109 cells requires superinfection, in which every cell has between 10 and 20 independent...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... combination of protein profiling and isotopomer analysis using matrixassisted laser desorption/ionizationtime of flight mass spectrometry reveals an active metabolism of the extracellular matrix ... 1998;Procollagen Nproteinase and procollagen C-proteinase Two unusual metalloproteinases that are essential for procollagen processing probably have important roles in development and cell signaling...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... also been reported to induce chondrogenic differentiation of ESCs by virtue of providing morphogenetic factors In the indirect route of differentiation, instead of starting the differentiation process ... Haematoxylin and eosin staining………………………………… 55 3.7.2.3 Alcian blue staining……………………………………………… 56 3.7.2.4 Safranin-O staining………………………………………………… 56 3.7.2.5 Masson’s trichrome staining……………………………………… ... 3.7.1 Staining of cell cultures…………………………………………… 51 3.7.1.1 Alcian blue staining……………………………………………… 51 3.7.1.2 Alkaline phosphatase (ALP) staining……………………………… 51 3.7.1.3 Alizarin red S staining……………………………………………...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... from roof plate cells in the dorsal neural tube and induce formation of neural crest stem cells and dorsal interneurons (Barth et al., 1999) Antagonists of Bmp signaling like chordin, noggin and...
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Identification of oct4 and sox2 targets in mouse embryonic stem cells
... Genome-wide Mapping of Oct4-DNA Interactions in Mouse Embryonic Stem Cells 4.1 Introduction 74 4.2 Results 76 4.2.1 Optimisation of large-scale ChIP 76 4.2.2 Global mapping of Oct4 binding sites in mESCs ... Genome-wide Identification of Sox2-DNA Interactions in Mouse Embryonic Stem Cells 5.1 Introduction 95 5.2 Results 96 5.2.1 Optimisation of ChIP and global mapping of Sox2 binding sites in mESCs 96 5.2.2 ... genome binding sites of Oct4 and Sox2 Thousands of novel Oct4 and Sox2 binding sites were identified, mapping to genes implicated in important cellular functions including maintenance of pluripotency...
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Regulation of gene expression by esrrb in embryonic stem cells
... finding indicates the embryonic origin of teratocarcinoma stem cells In 1974, the stem cells in teratocarcinomas were successfully isolated and defined as embryonal carcinoma (EC) cells (Martin ... differentiate into all cell types of the three germ layers of an organism Examples of pluripotent stem cells include the embryonic stem (ES) cells, embryonic germ (EG) cells and embryonal carcinoma (EC) cells ... cells Multipotent stem cells can give rise to cells of certain specialized lineages Many adult stem cells are multipotent, including hematopoietic stem cells, mesenchymal stem cells, and other...
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The role of kruppel like factors in embryonic stem cells
... transcripts zinc finger protein 42 zinc finger protein 143 zinc finger protein of the cerebellum XI Introduction CHAPTER I INTRODUCTION Introduction CHAPTER I INTRODUCTION Stem cells can be identified ... their binding to GTP or GDP Activated Ras leads to activation of a MAP kinase signaling cascade, including MAP kinase, MAP kinase kinase (MKK), and MAP kinase kinase kinase (MKKK) 16 Introduction ... domains Nanog protein contains domains, N-terminal domain (ND), homodomain (HD) and C-terminal domain (CD) CD is separated by a WR domain into CD1 and CD2 domain Different domains are indicated by...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... importance of Activin/ Nodal signaling in the maintenance of hESC pluripotency has been re-iterated in recent studies detailing the derivation and maintenance of induced pluripotent stem (iPS) cells ... pathway in differentiation of this multipotent lineage Activation of Notch signaling in combination with inhibition of Wnt signaling was shown to be responsible in part for the formation of cardiac ... clusters of cells called Islets of Langerhans which perform its endocrine functions Acinar cells secrete digestive enzymes like trypsin and chymotrypsin into the small intestine Islets of Langerhans...
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