... HIDETOSHI YAMAZAKI, AND SHIN-ICHI HAYASHI 98 v vi TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from EmbryonicStemCells The InVitro Differentiation of Mouse EmbryonicStemCells ... VICTORIA L BAUTCH 83 InVitro Differentiation of Mouse ES Cells into Hematopoietic, Endothelial, and Osteoblastic Cell Lineages: The Possibility ofInVitro Organogenesis MOTOKAZU TSUNETO, TOSHIYUKI ... SAKAI-OGAWA, TORU NAKANO 72 InVitro Differentiation of Mouse EmbryonicStemCellsto Hematopoietic Cells on an OP9 Stromal Cell Monolayer AND InVitro Differentiation of Mouse ES Cells: Hematopoietic...
... this inflammatory and catabolic cascade and demonstrated that curcumin (6) has the capacity to block the action of pro-inflammatory cytokines in the joint thus disrupting the inflammatory cycle inhibit ... leading to enhanced degradation of the ECM and induction of pain [8] Additionally, in articular chondrocytes, NF-κB stimulates the production of pro-inflammatory catabolic cytokines, which induce ... inhibition of IκB kinase Page of 15 [13,18] There is increasing interest in curcumin as a therapeutic option for OA and RA, with evidence that curcumin inhibits the IL-1β-induced activation of...
... effective methods to verify stemness and monitor quality in such cells are also needed to bring the full range of therapeutic possibility into focus In an effort to develop an assessment system for ESCs, ... other investigators have noted that it is not currently possible to transform a single blastomere into stemcells without recourse to formation and intentional destruction of whole blastocysts ... Ratio of inner cell mass and trophoblastic cellsin blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured invitroin the presence or absence of protein and...
... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving ... 2003;Selective adhesion of osteoblastic cellsto different integrin ligands induces osteopontin gene expression Matrix Biol (3):241-249 135 Spiegelman BM, Ginty CA 1983;Fibronectin modulation of cell shape...
... uniqueness of the epigenetic profile in ES cellsIn contrast to ES cells, which maintain the ability to differentiate into all cell types of the embryo proper [10], trophoblast stem (TS) cells originate ... from the trophectoderm of blastocysts and can differentiate only into placental cellsin vivo and invitro [11] Differentiationofcells from the early blastomere stage to the blastocyst stage is ... (ICM) of blastocysts and EG cells derived from the primordial germ cells (PGCs) in developing genital ridges can develop into cellsof the embryo proper, after they are injected into blastocysts to...
... the role of integrins α4, α7, β5 and β7 during chondrogenesis Integrins containing the α4 or α7 subunit bind to RGD-containing components, namely fibronectin and vitronectin or laminin, respectively ... integrins α1, α2, α3, α5, α6, α10, αv, β1, β3 and β5 [52] Integrin α5β1 is the most prominently expressed integrin in chondrocytes and integrin α10β1 is the dominant collagenbinding integrin during ... protein (COMP), dermatopontin and fibronectin, or are part of the cytoskeleton, such as filamin [17] Other proteins, such as osteonectin and osteopontin, are involved in the mineralization of cartilage...
... participates in the initiation of ES commitment into embryonic cell lineages List of abbreviations ESC: embryonicstem cell; FGF: fibroblast growth factor; MAPK: mitogen-activated protein kinase; SFEB: ... Yoshimura D, Tominaga Y, Nishioka T, Ito M, Nakabeppu Y: Invitro development of mouse embryonicstemcells lacking JNK/ stress-activated protein kinase-associated protein (JSAP1) scaffold protein revealed ... Nuclei of all cells are revealed by DAPI staining in blue Scale bar, 10 μm in C *, p < 0.01, Anova test into neuroblast cells Immunocytostaining revealed that both FGFR1 and FGFR3 were detected in...
... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... directing the differentiationofstemcells into the osteogenic lineage invitro J Bone Miner Res 2004, 19:1379-1394 Page of 38 Trounson A: Human embryonicstem cells: mother of all cell and tissue ... resemble aggregates consisting of preosteoblasts Our finding of a mineralization in microspheres of DAG-treated ESCs seems to share similarity to the formation of bone and dentin in vivo Thus, it appears...
... Kossa stainings with arrows pointing to the deposition of calcium phosphate-containing minerals that stained in black Figure LIF, stem cell PCR showing amplification of the without hematopoeticqualitative ... stemcellsin tissue engineering of bone [17,18] Embryonicstemcells (ESCs) are routinely derived from the inner cell mass of blastocysts and represent pluripotential embryonic precursor cells ... leukemia inhibitory factor (LIF) [22,23] In the absence of LIF embryonicstemcells will differentiate into a morphologically mixed cell population expressing features of endoderm and mesoderm lineages...
... attributed to many factors such as ratio of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number ofstem cell passages and site of injection; ... Hematopoietic Cells as Hepatocyte Stem Cells: A Critical Review of the Evidence Hepatology 2006, 43:2-8 49 Jang YY, Collector MI, Baylin SB, et al: Hematopoietic stemcells convert into liver cells ... tissues by assessing the gene expression profile of some of the Wnt signaling target genes:cyclin D, PCNA, survivin, b-catenin Methods Ninety albino female rats inbred strain (Cux1: HEL1) of matched...
... at 37°C in a humidified atmosphere containing 5% CO2, while μg/mL doxorubicin was contained in the culture to maintain K562/AO2 cells drug resistance in its daily culture The inhibition of cell ... prepared according to the previous report [30], in which 100 mL of 0.01% HAuCl4 was heated to boiling with uninterruptedly agitating and 3.0 mL of 1% trisodium citrate was dropped into the above ... K562/AO2 cells than that of K562 cells, as shown in Figure 4a and 4b Interestingly, Page of the presence of PLA/Au nanocomposites could efficiently facilitate the drug uptake of DNR into the drug-resistant...
... Figure Apoptosis induction by isoproterenol or propranolol in combination with irradiation in BGC-823 and SGC-7901 cells There was an increasing rate of apoptosis in gastric cancer cell lines in response ... activity in cancer cells, thus making the cells radioresistant [12] Activation of NF-B by various stimuli, including inflammation, stress and radiation, involves degradation of the inhibitory subunit ... effect of propranolol on the induction of apoptosis was examined using flow cytometric (FCM) analysis with Annexin V-PI staining After propranolol pre-treatment (50 μM for 24 hr) and following irradiation,...
... combination of goserelin with radiotherapy in PC cannot be attributed to an increase in radiosensitivity of PC cells by goserelin invitro It is to be explained by the synergistic effects of AD ... moderate induction of apoptosis by irradiation in this cell line (see discussion) leading to an insignificant reduction in the number of viable cells PC-3 Also in PC-3 cells an exponential growth kinetic ... behaviour of exponentially growing cells Results Staining of LHRH-receptors The studied passages of both cell lines LNCaP und PC-3 showed a high expression of LHRH receptors In figure receptor expression...
... seasonal influenza viruses in its induction of cytokine responses in human macrophages and epithelial cells [2-4] This suggests that the cytokine dysregulation seen in H5N1 infection is not an intrinsic ... multiplicity of infection (MOI) of two Minimum Essential Medium (MEM) (Gibco) with 100 U/ml penicillin and 100 μg/ml streptomycin was used as inoculum in the mock infected cells The cells were incubated ... further investigation Lack of the control of transcriptional suppression by zinc finger proteins in pdmH1N1 infected cells We found that nine of the genes down-regulated in response to seasonal...
... obtained after incubating cells with GSH (1.25 – mM) instead of NAC (data not shown) In contrast, in NCI-H292 cells but not in PBEC, depleting GSH using BSO resulted in a full inhibition of wound ... decrease in GSH, but did not increase GSH in the absence of CSC This finding is in line with previous observations, showing that NAC does increase total non-protein thiols but does not increase ... closure in cultures of bronchial epithelial cells These invitro results may add new elements to our insight into the pathogenesis of smoking-induced lung injury, and more specifically to the epithelial...
... histone modification marks to the b-globin gene locus during erythroid differentiationof murine ES cellsinvitroIn these experiments we utilized the ES ⁄ OP9 cell invitrodifferentiation system ... chromatin-opening activity In other words, HS3 could maintain a small accessible region in the globin locus during the differentiationof hematopoietic stemcellsto erythroid cells Transcription of HS3 ... nature and extent of covalent histone modifications and association of transcription complexes at the murine b-globin locus during the invitrodifferentiationof murine embryonicstemcells We observed...
... characterization of transplanted cells derived from human ES cells We induced differentiationof human ES cellsin an invitro two-dimensional culture on OP9 stromal cell line and examined the expression of ... purposes) Journal of Translational Medicine 2008, 6:54 cells, the number (cells/ mm2/mouse) of single stranded DNA (ss-DNA)+ cellsin one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected ... transplanted cells were incorporated into the host circulation [3] Human CD34+ cells isolated from umbilical cord blood were found to be capable of secreting several angiogenic factors, including VEGF,...
... expression inembryonicstemcells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific miRNA is a major obstacle in understanding the miRNA ... differentiationof olfactory progenitor cellsin zebrafish model [51], indicating that the miR-200 family is involved in development It has been shown that the inhibition of miR-141 decreases growth of cholangiocarcinoma ... MicroRNA Discovery and Profiling in Human EmbryonicStemCells by Deep Sequencing of Small RNA Libraries StemCells 2008, 26(10):2496-2505 Seitz H, Royo H, Bortolin ML, Lin SP, Ferguson-Smith AC,...
... determined the H2O2-induced ROS generation in mouse -5 embryonicstemcells using DCF-DA (10 M), a fluorescent probe of intracellular H2O2 Production of ROS was -3 detected in the cells exposed to ... regulation To investigate the effect of DHT on H2O2-induced increase of cell cycle regulatory protein expression, the mouse ES cells were pretreated with DHT for 30 prior to adding the H2O2 The H2O2-induced ... Effect of dihydrotestosterone on mouse embryonicstemcells exposed to H2O2-induced oxidative stress 255 10, 423-426 12 Brook FA, Gardner RL The origin and efficient derivation ofembryonicstem cells...