differentiation of embryonic stem cells into endoderm derived hepatocytes

differentiation of embryonic stem cells

... TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Diﬀerentiation of Mouse Embryonic Stem Cells into Neutrophils 10 Development and Analysis of ... from Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Diﬀerentiation of Embryonic Stem Cells 13 ... Section I Differentiation of Mouse Embryonic Stem Cells [1] Lineage Specific Differentiation of Mouse ES Cells: Formation and Differentiation of Early Primitive Ectoderm-like (EPL) Cells By JOY...

574
619
0

Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages

Danh mục: Tổng hợp

... stages of embryonic development from fertilization to E6.5 are represented in this image Embryonic Stem (ES) cells are derived from the inner cell mass while epiblast stem cells (EpiSCs) are of epiblast ... analysis of developmental events was made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of these cells has led to the development of ... ability of two cell lines– hES2 and hES3– to generate derivatives of mesoderm and endoderm in vitro Differentiation into these particular lineages was of interest to me as hESC -derived β-like cells...

296
2,020
0

Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Danh mục: Báo cáo khoa học

... Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole embryos or compromising extant ... Human embryonic stem cells: theoretical approaches Blastomere biopsy and culture Prior to the blastocyst stage, a human embryo at 2–3d post fertilization consists of just 4–8 cells, all of which ... scoring system Stem cell research: social and political factors Public sharing of information about the basic science of ESCs has proven to be important, since those who are aware of the stem cell...

8
368
0

Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx

Danh mục: Báo cáo khoa học

... embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells derived from ... establishment of embryonic stem (ES) cells, which maintain the ability to form all types of cells in the body, and can differentiate into a variety of cell types in vitro [8] The use of ES cells in ... ED between ES cells and EG cells, the ED of TS cells to ES cells (77) is greater than that of EG cells to ES cells (49), conﬁrming the similarity of EG cells to ES cells DNA methylation proﬁles...

7
439
0

Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt

Danh mục: Báo cáo khoa học

... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...

6
410
0

Báo cáo y học: "Microenvironmental changes during differentiation of mesenchymal stem cells towards chondrocytes" ppt

Danh mục: Báo cáo khoa học

... chondrogenic differentiation of mesenchymal stem cells (MSCs) The gene-expression (MSCs) profile of mesenchymal stem cells (MSCs; n = 2) was analysed by real-time PCR during their differentiation towards ... Figure Time-course expression of chemokines and their receptors during chondrogenic differentiation of mesenchymal stem cells (MSCs) The gene(MSCs) expression profile of MSCs (n = 2) was analysed ... metalloproteinases in the differentiation and biological 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 characterization of mesenchymal stem cells Stem Cells 2006, 24:475-481 Charo IF, Ransohoff RM: The...

12
390
0

The signals of FGFs on the neurogenesis of embryonic stem cells ppsx

Danh mục: Báo cáo khoa học

... promotes self-renewal of mouse embryonic stem cells Dev Biol 1999, 210:30-43 Li Z, Theus MH, Wei L: Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells Dev Growth ... Derivation of midbrain dopamine neurons from human embryonic stem cells Proc Natl Acad Sci USA 2004, 101:12543-12548 Ying QL, Stavridis M, Griffiths D, Li M, Smith A: Conversion of embryonic stem cells ... detect the Sox1-GFP+ cells from day to day (Fig 3A) The number of Sox1+ cells became 20% of total cells on day and reached the plateau, 50% of total cells, on day Treatment of FGF1 consistently...

11
194
0

báo cáo khoa học:" Induction of osteogenic markers in differentially treated cultures of embryonic stem cells" ppt

Danh mục: Báo cáo khoa học

... autologous cells as well as allogenic and xenogenic cells [13-16] There are some reports that use totipotential embryonic stem cells in tissue engineering of bone [17,18] Embryonic stem cells (ESCs) ... mouse embryonic stem cells Cloning Stem Cells 2004, 6(4):386-391 Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, Rogers D: Inhibition of pluripotential embryonic stem cell differentiation ... mineralization of osteogenic cells derived from human embryonic stem cells Tissue Eng 2004, 10(910):1518-1525 Evans MJ, Kaufman MH: Establishment in culture of pluripotential cells from mouse...

7
397
0

Báo cáo y học: " Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment" pdf

Danh mục: Báo cáo khoa học

... Characterisation of canine adipose tissue derived mesenchymal stem cells In order to demonstrate that the cells isolated from the canine adipose tissue (Figure 1A) are indeed mesenchymal stem cells (MSCs) ... effects of curcumin inhibit the induction of degradative and inflammatory pathways by IL-1β through revoking activation of caspase-3, production of COX-2, phosphorylation of Iκ-Bα and inhibition of ... inhibits chondrogenic differentiation of MSCs in OA and the effect of curcumin Trauma, inflammation or a combination of both (1) lead to the production and accumulation of high levels of pro-inflammatory...

15
328
0

Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

Danh mục: Báo cáo khoa học

... erythroid differentiation of murine ES cells in vitro In these experiments we utilized the ES ⁄ OP9 cell in vitro differentiation system described by Kitayima et al [20] The ability of these cells ... both hematopoi- Fig Sequential activation of globin gene transcription during in vitro erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed ... Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin gene during differentiation...

10
422
0

báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx

Danh mục: Hóa học - Dầu khí

... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of ... VE-cadherin (+) cells VE-cadherin (-) aSMA (+) cells hES -ECs hES -ECs+MCs aSMA (+) cells hES -MCs Figure cells differentiated from human of the vascular Schematic representation of preparationES cellstransplanted ... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs),...

14
450
0

báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc

Danh mục: Hóa học - Dầu khí

... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... expression profiling of single whole embryonic stem cells Nucleic Acids Res 2006, 34(2):e9 Chen C, Ridzon D, Lee CT, Blake J, Sun Y, Strauss WM: Defining embryonic stem cell identity using differentiation- related ... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine...

17
593
0

Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx

Danh mục: Báo cáo khoa học

... vitro differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved ... infected cells for 48 hours Results FN and LAM enhance differentiation of MSCs into insulin producing cells To examine the effects of ECM, FN and LAM on IPC differentiation by MSCs, a four-stage differentiation ... stromal cells differentiate into neural cells in vitro Exp Neurol 2000, 164(2):247-256 Lumelsky N, Blondel O, Laeng P, Velasco I, Ravin R, McKay R: Differentiation of embryonic stem cells to...

10
332
0

Báo cáo y học: "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions" pdf

Danh mục: Báo cáo khoa học

... triggers differentiation of HUMSCs into cardiomyocytes and maturation of HUMSCs derived cardiomyocytes Admittedly, the detailed mechanism(s) of above effects of S1P on differentiation of and maturation ... with Regard to the Source of Fetus -Derived Stem Cells Stem cells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem cells from whole human umbilical ... fluorescent immunostaining of aactinin of cells from three groups, while, J-L shows the fluorescent immunostaining of MHC of cells from these groups after and 10 days’ culturing Cells from CMCM and...

9
262
0

báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

Danh mục: Báo cáo khoa học

... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of ... epigenomic profiling of a cohort of human embryonic stem cell (hESC) lines can be used to generate hESC reference epigenomes (left panels) The extent of reprogramming and differentiation potential of individual ... cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; iPSC,...

13
338
0

Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration

Danh mục: Cao đẳng - Đại học

... analysis of hESC -derived chondrogenic cells xv Fig 20 Cell cycle kinetics of hESC -derived chondrogenic cells Fig 21 Genome integrity of hESC -derived chondrogenic cells Fig 22 Tumorigenicity of hESC -derived ... chondrogenic cells Fig 23 ECM modulation of post-expansion differentiation capability of hESC -derived chondrogenic cells Fig 24 Response of hESC -derived chondrogenic cells to anabolic growth factors of ... chondrogenic cells ………………………… 83 PHASE III: ISOLATION OF CHONDROGENIC CELLS …………… 86 4.3.1 4.3.2 Expansion of hESC -derived chondrogenic cells …………………88 4.3.3 Differentiation capability of hESC-derived...

190
471
0

Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"

Danh mục: Y học thưởng thức

... Marshall VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from ... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton ... Assessment of spontaneous differentiation of hESC colonies after exposure to reduced temperature After exposure to low temperature, the hESC were incubated at 37oC for to 2h in the presence of fresh...

6
477
0

EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES doc

Danh mục: Sức khỏe giới tính

... sources of pluripotent stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In ... differentiation of cells derived from human embryonic stem cells Proc Natl Acad Sci U S A, 2000 97(21): p 11307-12 [20] Kroon, E., et al., Pancreatic endoderm derived from human embryonic stem cells generates ... contexts with respect to stem cell differentiation: maintenance of pluripotent stem cells and tumorigenesis of cancer stem cells In the first context, inhibition of PTEN, activation of PI3K and Akt/PKB,...

518
390
1

EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES pdf

Danh mục: Sức khỏe giới tính

... sources of pluripotent stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In ... differentiation of cells derived from human embryonic stem cells Proc Natl Acad Sci U S A, 2000 97(21): p 11307-12 [20] Kroon, E., et al., Pancreatic endoderm derived from human embryonic stem cells generates ... contexts with respect to stem cell differentiation: maintenance of pluripotent stem cells and tumorigenesis of cancer stem cells In the first context, inhibition of PTEN, activation of PI3K and Akt/PKB,...

518
442
0

Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf

Danh mục: Báo cáo khoa học

... in mouse -5 embryonic stem cells using DCF-DA (10 M), a fluorescent probe of intracellular H2O2 Production of ROS was -3 detected in the cells exposed to H2O2 10 M for a variety of times (0-60 ... role of dihydrotestosterone in benign prostatic hyperplasia Urology 2003, 61 (Suppl 1), 2-7 16 Chambers I The molecular basis of pluripotency in mouse embryonic stem cells Cloning Stem Cells ... on mouse embryonic stem cells exposed to H2O2-induced oxidative stress 253 Fig Effect of dihydrotestosterone (DHT) on hydrogen peroxide (H2O2)-induced decrease of DNA synthesis A-D: The cells were...

10
263
0