... microarrays, where adding on tags such as biotin to a protein allow it to be specifically immobilized onto an avidin-coated surface Differentapproachesto protein engineering and modulation using ... where adding on tags like biotin to a protein allows it to be specifically immobilized onto an avidincoated surface [10, 11, 22] 1.1.3 The three differentapproachesto protein engineering and modulation ... Sortase Cloning of Sortase A into the pTOPO vector MCS 10B The T7 genome-10-3-B vector 10B MCS Phage heads RE digest of the 10-3-B vector with BamH1 and XmaI Packaging into the T7 phage packaging...
... the final step is to join them into the object WWTP With mathematic models brought into WWTP software, and software combined into the monitoring and control system, the monitoring and control ... still many things toto before it works in the real WWTP The mathematic models should be adjusted to real plant behavior, which means to adjust the parameters and calibrate the model to adapt the ... correspond to the sensors It also needs to some programme to achieve the data transfer from sensors or apparatus to the control system After the whole network is established, we still need to test...
... sleep To pass away To lose somebody To expire To kick the bucket To be at rest To pop off To rest in peace To drop dead To lose one s life To snuff it To lay down one s life To give up the ghost To ... as: To pass away To be near one s end To decease/deceased To lay down one s life To be gathered to one s fathers To lie/ to be at rest To rest in peace To go to glory/Heaven To join the angles To ... such a great fear to talk about death because to die means to stop existing Different people creat different ways to mention about death; it might be a direct way: to die, to perish Or might...
... allow students to practice language skills (Lee, 1995) Games encourage learners to interact and communicate with one another When taking part in the games learners have to talk to express or ... your classmates help you to learn vocabulary through games? a b c d Help you to guess unknown words Help you to recall the words you have learnt but forgotten Help you to remember words from the ... bringing fun to classes Games can provide language practice Games encourage learners to interact and communicate with one another Games can motivate learners by bringing fun to classes Games...
... growth monitoring Multi-sectoral platform: Linking health efforts to those of other sectors in order to address determinants of ill health and sustain improvements in health The multi-sectoral platform ... people want to ‘see’ what one looks like in the field Documentation of differentapproachesto implementation of the three Elements is crucial, and will allow program planners to appreciate ... women come together in a Care Group every two weeks to learn life-saving health messages from a health educator Each woman is then responsible to teach the health lessons they learn to 10–15 of...
... and noninhibitory conformation Glyphosate sensitivity is partly restored by mutation of Ala100 to glycine, allowing glyphosate to bind in its extended, inhibitory conformation Detoxification of ... subtilis was also shown to convert glyphosate into AMPA and glyoxylate, but with a reaction mechanism different from that of GOX Oxidases GOX (Monsanto) Early on, Monsanto Co isolated glyphosate-AMPA ... substitution of Gly96 to Ala and Ala183 to Thr in 5-enolpyruvylshikimate-3-phosphate synthase gene of E coli (k12) and transformation of rapeseed (Brassica napus L.) in order to make tolerance to glyphosate...
... common to organic synthesis but not yet amenable to protein modification As new methodologies emerge, the study of other PTMs and that of regulatory PTMs will hopefully provide a powerful tool for ... degree of branching cooperate to regulate cell proliferation and differentiation Cell 129, 123–134 16 Comstock LE & Kasper DL (2006) Bacterial glycans: key mediators of diverse host immune responses ... pastoris Nat Biotechnol 24, 210–215 Choi B-K, Bobrowicz P, Davidson RC, Hamilton SR, Kung DH, Li H, Miele RG, Nett JH, Wildt S & Gerngross TU (2003) Use of combinatorial genetic libraries to...
... required DBMS-X to be running in order to adjust them, it was unfortunately easy to lock ourselves out with no failsafe mode to restore to a previous state Vertica was relatively easy to install as ... required us to (1) write to a custom tuple object using Hadoop’s API, (2) modify our data loader program to transform records to compressed and serialized custom tuples, and (3) refactor each benchmark ... it is then trivial to use a second query tooutput the record with the largest totalRevenue field SELECT INTO Temp sourceIP, AVG(pageRank) as avgPageRank, SUM(adRevenue) as totalRevenue FROM Rankings...
... long day), we want to be able both to understand what makes proteins ÔtickÕ and to engineer proteins with native-like properties In this review we discuss combinatorial approaches toward understanding ... function can be tied to the survival of a cell or some easy -to- observe phenotypic property Ultimately, one would like to be able to study proteins whose There is no straightforward way to identify a ... sequence resemblance to each other or to proteins known in nature [69] However, these proteins were not sufficiently soluble to be examined in vitro except as fusions to maltose binding protein...
... hypothesis is supported by different a-conotoxin sequences (Table 1), different a-conotoxin kinetics [32–35], and finally, the involvement of different nAChR residues from different regions of nAChR ... determinants have been mapped and a-conotoxins appear to bind mainly to loop C, but also clearly extend to microsites above this loop and on the b9b10 hairpin Indeed, a-conotoxins act at the competitive ... subtype, thanks to the large amount of the receptor obtained from the electric organ of the Torpedo marmorata ray and availability of selective pharmacological tools like the snake a-neurotoxins The...
... territory Civil registration is an official record of a person’s existence, including the right to a name, the right to be registered at birth, the right to belong to a family, the right to nationality, ... Georgetown University, Washington DC Amy Liu, Deputy Director, Metropolitan Policy, Brookings, Asset-based Approachesto Katrina Disaster and Reconstruction 34 Lilianne Fan, Advocacy Coordinator, ... paper provides a brief introduction to asset-based approachesto poverty reduction in a globalized context The aim is to show the added value of asset-based approaches, in terms of both better...
... exclusively in rubber Exports from Laos to China are expected to increase mainly due to rubber, with both countries seeking to increase trade profits to at least $1 billion USD over the next ... shows how different actors at village level (villagers, district authorities, rubber company staff, Welthungerhilfe project staff, and other „hinterland actors‟) clash with respect todifferent ... area have to sell their yields to the Thien Loui Ye Company Ltd Therefore, the farmers are deprived of a significant alternative to contract farming Altogether, the instructions attached to the...
... monitoring of progress towards MDG target C: C: to halve, by 2015, results the global monitoring of progress towards MDG 7 target to halve, by 2015, results of the global monitoring of progress towards ... Ghana) or lack indicators to assess performance of the instruments (China) This lack of monitoring tools to assess the performance of indicators will continue to impact the ability to measure progress ... APPLICATION OF INTEGRATED APPROACHESTO WATER RESOURCES MANAGEMENT This chapter reports the extent to which countries have been able to apply tools and methods, often referred to as “management instruments”...
... transfer A Review of Approachesto Mobility Telemonitoring Telemonitoring data logging systems allow the person to be monitored regardless of their location and allow complex analysis to be performed ... have the capability to transfer their data, for long- term storage and analysis, to a remote monitoring center Data can be transmitted directly from the wearable to the monitoring center using ... required to remember to don the wearable unit for their mobility to be measured (Table 5) Though, it gives the possibility to the person to doff the wearable for a while and still be monitored by...
... RAND undertook a five-year progress assessment of the CERI effort that had three purposes: to provide feedback to sites to improve their efforts, to provide information to the foundation to inform ... site-specific issues In addition to the protocols, we had a list specific to each of the documents and evidence to be collected To the extent possible, we attempted to track progress of the unique ... Development of Indicators 21 laborative gained stature or “voice” in the community such that it brought different CBOs together to work more effectively to ensure supportive policy or to participate...
... curves to putative solutions of the equations Fn = y p and Ln = y p with even index n to Frey curves and apply level-lowering This, together with some elementary arguments, is used to reduce to the ... for variables, of solutions to exponential Diophantine equations (III) To show how local information obtained through the modular approach can be pieced together to provide a proof that there ... following: (I) To present theoretical improvements to various aspects of the classical approach (II) To show how local information obtained through the modular approach can be used to reduce the...
... “not for tomorrow – but for today” to promote and revive traditional roles, cultural values and to work with the Elders in rejuvenating the traditional approachesto healing, as well as to provide ... heidibartjohnston@gmail.com 10 SOCIAL DETERMINANTS APPROACHESTO PUBLIC HEALTH: from concept to practice Abstract Every year, globally, an estimated 66 500 women die attempting to terminate a pregnancy To ... doctors, FWVs and a few private doctors (Akhter, 1988) American medical consultants came to Bangladesh to train providers in the use of manual vacuum aspiration, and doctors were also sent to...
... Symptoms of local spread Into nasal cavity: Unilateral epistaxis Into orbit: Ocular dysfunction, proptosis, diplopia Into oral cavity: Pain/loosening of upper teeth; “dentures don’t fit” Into ... usually asymptomatic Most common in frontal and ethmoid Rare in maxilla! 2:1 male -to- female ratio Complications: Extension into nose: nasal obstruction/swelling Extension into orbit: proptosis External ... patient: Mr R Common signs/symptoms of sinus disease Radiological Menu of Tests Normal anatomy Differential diagnosis Radiological findings Companion cases Putting it all together: Mr R Nir Harish,...
... to the store yesterday and cooked lasagna today.” A system might return erroneous Who and What answers, and return Where as to the store” and When as “today.” Since Where and When apply todifferent ... For each 5W output, annotators were presented with the reference translation, the MT version, and the 5W answers The 5W system names were hidden from the annotators Annotators had to select “Correct”, ... addition to judging the system answers by the task guidelines, annotators were asked to provide reason(s) an answer was wrong by selecting from a list of predefined errors Annotators were asked to...