... EnglandBiolabs) and the tetracycline-resistance cassette into theHindIII site. pRS239 was obtained by inserting the tetra-cycline-resistance cassette into the HindIII site of pRS155,encoding ... primer (NifLE-coRI); 5Â-CAATGTCCTGAAGCTTACATAAGGCTTCAC-3Â, antisense primer (NifAHindIII). The 3.1 kb PCRproduct was cloned into t he EcoRI and HindIII sites ofpMAL -c2 , resulting in malE fused ... (pKK2233R,5Â-GATGCCGGCCACGATGCG-3 Â). This 0.4 kb PCRfragment was cloned into the ScaI site located inside theampicillin re sistance gene (bla) in pRS163, p RS98, and pRS205 re sulting in pRS180 (MBP-NifL...
... bityrosine signal was observed in allpeptides containing tyrosine 23 or 53 but not in peptidescontaining tyrosine 20 alone. According to these results,tyrosines 23 and 53 could be involved in ... Moshe Faraggi3,Ce´cile Sicard-Roselli1, Chantal Houe´e-Levin1 and Pierre Jolle`s41LCP, Centre Universitaire, Orsay Cedex, France;2Institut Curie-Section de Recherche, Centre Universitaire, ... theintramolecular electron transfer.This approach complements other studies, directed atunderstanding the electron transfer process, which involveschanging the protein sequence by site-directed mutagenesisand...
... Predicate Linear Inequality Linear Inequality. Product Linear Inequality Product Time Complexity trivial O(pc), O(pc), O(pc)t O(pc), O(pc) t Completeness Complete Incomplete Incomplete ... result of executing R-(Xi, C) and n+ ( xi , C) on L for each Xi whose label was changed for each constraint C& apos; ~ Cin which Xi appears add C I to Q end Since refinement is a sound ... are differenceand factor terms in the linguistic material to be processed? If differenceand factor terms are so marginal that they may not occur at all, then the non- compositional approach...
... surface glycoproteins: hemagglutinin-neuraminidase (HN) glycoprotein and fusion protein (F protein) [1]. HN glycoprotein shows multiple biological functions that include hemagglutinin and enzymatic ... types 1–4 mainly infect younger children at least once, although reinfections may occur in adults [2,3]. Indeed, serological surveys indicate that at least 75% of children have been infected with ... detected strains in each cluster on the phylogenetic tree was as follows: cluster 1, 84 strains including strains isolated during 2004–2009; and cluster 2, 95 strains from 2003 to 2005, and...
... Countries, China, Northern Korea, Cuba. Vietnam and Mongolia ACADEMIA, Publishing House of the Czechoslovak Academy of Sciences. Prague. Czechoslovakia for all remaining areas ELSEVIER SCIENCE ... organisms, and compares them with modern ecological terminology.There is a similarity in meaning between auto- chthonous and oligotrophic, and zymogenous and copiotrophic, but the terms certainly ... electronic, mechanical, photocopying. recording. or otherwise, without the prior written permission of the copyright owners. Printed in Czechoslovakia. CRITERIA OF ROOT-NODULE BACTERIA ACTIVITY...
... Practices and Socio-Economics of Shrimp Farming in Tra Vinh and Ca Mau provinces,Vietnam, A report submitted in partial fulfilment of GAMAS Project’, Ho Chi Minh, Research Insti-tute of Aquaculture ... area occupied by rice-based farming systems in the Cai Nuocdistrict did not change much in a 30-year time span, 49,721.68 ha in 1968,529LAND COVER CHANGES IN CAI NUOC 3.2. The 1968 Land Cover ... Modelling and Analysis in Coastal Zones: Principles and Practices, Texel, Netherlands, Land-Ocean Q Interactions in thecoastal zone projects, reports and studies No. 11.Valiela, I., Bowen, J.L. and...
... containany cycle, hence is a forest. A path with edges in T joining two distinct vertices of Vproduces a linear relation between the corresponding hyperplanes. Hence, a connectedcomponent of T contains ... – and tothe path delimited by 4 and d. The corresponding active path has two edges 4 and a.The construction of α canbedonebyfirstconsideringthepathinducedbyd, b, c, since the flag 1bcd ⊂ 1bcd2aef ... a classical bijection between permutations and increasing spanning trees [7],[9],[25] (see [23] p. 25), and also another construction ofthe bijection of [11] between trees and acyclic orientations...
... seedling and relatively in- dependent of climatic hazards becausethey were obtained in different years (withdifferent patterns of temperature and radiation in this continental ... walnutseedlings. Since there was no relationship between bud dormancy and rootgrowth, the reasons for the cessation ofroot growth might be related to carbonallocation or to climatic ... climatic factors, such assoil or temperature (or both).The specific relationships observed be-tween the growth variables during the 2growing seasons were characteristic ofthe...
... transposition reaction in DH5α and selection on plates containing kanamycin (cassette in themini-transposon) and spectinomycin (cassette in the targetvector), target plasmids with an inserted mini-transposon ... insertions in an archived collection of mutants. The concentric circles show (reading inwards): insertions in genes (green); genes transcribed in the clockwise direction (red); genes transcribed in ... importantas tatA/E in FAM18, which encodes the TatA/E component ofthe Sec-independent protein translocase, ccoQ in MC58,which encodes one of the components of cytochrome c oxi-dase, and as many as...
... evening active cells as thedays get longer in spring [38,39], the coincidence effectof the morning and evening active cells disappearingwhen the melatonin signal duration becomes insufficientto ... Helsinki, Finland,for their help in data processing.The Finnish Cultural Foundation, Finnish National Graduate School forClinical Investigation, and Finnish Graduate School of Psychiatry allocatedscholarships ... suic ide mortality and confidence interval of the mean in aggregate over the years from 1969 to 2003 for men and women, duringastronomical seasons in Finland, Helsinki and Oulu, and according...
... better understanding of characteristics of leu-kemia in Nanjing, including etiology, survival and riskindicators, which may lend support to the correspondingprotocols for prevention and intervention.AbbreviationsFAB: ... intervention.AbbreviationsFAB: French-American-British Cooperation Group; ALL: acute lymphocytic leu-kaemia; AML: acute myeloid/myelogenous leukemia; CLL: chronic lymphocyticleukemia; CML: chronic myelocytic leukemia; ... histochemical staining and typing and classi-fied by the "French American British" classification. Thepopulation data were provided by Nanjing Public Secu-rity Bureau and Nanjing Statistical...
... basic mechanism of glucocorticoidactions is that they penetrate into the cell and bind to glu-cocorticoid receptor molecules in the cytoplasm.[19,20]The glucocorticoid- glucocorticoid receptor ... complexacts as a transcription factor, binding to specific DNA sites in the nucleus. Within the nucleus, GR may induce genetranscription (transactivation) by binding to specific DNAsequences ... stimulatingfactorGR: Glucocorticoid receptorGRE: Glucocorticoid response elementIL: InterleukinMCP-1: Monocyte chemoattractant protein 1NF-κB: Nuclear factor κBCompeting interestsThe author(s) declare that...