diergism gonadal hormones and dopamine
Báo cáo khoa học: Production and utilization of hydrogen peroxide associated with melanogenesis and tyrosinase-mediated oxidations of DOPA and dopamine docx
... HPLC-ED to determine if, and to what extent, H2O2 was generated during the autoxidations and tyrosinase-mediated oxidations of tyrosine, DOPA, and dopamine This sensitive and specific method was ... autoxidation and enzyme-mediated oxidation of DOPA (Fig 5) and dopamine (not presented), but not in reaction mixtures containing catalase After incubation, lL samples were removed and analyzed ... dopamine following the addition of varying amounts of the diphenol into solutions of NaCl ⁄ Pi (pH 7.4) that lacked catalase (A and B) and those with catalase (C) Arrows indicate times when dopamine...
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Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx
... induction with IPTG) preincubated with either no ligand (d), 40 lM H4biopterin (m), 40 lM dopamine (n), 40 lM H4biopterin and mM L-Phe (j), 40 lM dopamine and mM L-Phe (h) or mM L-Phe (s) before being ... H4biopterin and substrate (PDB id code 1KWO) and (D) binary hPAH with bound dopamine (PDB id code 5PAH) The backbone of rPAH regulatory domain and hPAH catalytic domain are shown in red and green, ... structures of hPAH and rPAH [1621] have provided the basis to further explore the molecular mechanism by which the pterin cofactor and dopamine function as negative effectors and how substrate...
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báo cáo hóa học: " CD200-CD200R dysfunction exacerbates microglial activation and dopaminergic neurodegeneration in a rat model of Parkinson’s disease" pot
... Measurements of dopamine and its metabolites by HPLC Animals (n = each of the following groups: 6-OHDA/ Veh, 6-OHDA/CAb, and 6-OHDA/BAb) were sacrificed by CO and their brains were quickly removed and placed ... links between CD200/CD200R signalling and PD, Alzheimer’s disease (AD) and prion diseases Protein and mRNA levels of CD200 and CD200R are decreased in hippocampus and inferior Zhang et al Journal ... TNF-a and IL-6 Rats were killed by CO2 overdose followed by cervical dislocation and decapitation at 21 days after injection of 6-OHDA The brain was removed and immediately transferred to ice and...
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Section XII - Hormones and Hormone Antagonists pps
... (seeBraverman and Utiger, 2000; Braverman and Refetoff, 1997) Thyroid The thyroid gland is the source of two fundamentally different types of hormones The iodothyronine hormones include thyroxine (T4) and ... their affinity and specificity for their ligands The FSH and LH receptors couple with Gs to activate adenylyl cyclase and raise the intracellular level of cyclic AMP At higher ligand concentrations, ... peritoneal cavity, thorax, and even the pericardium Signs and symptoms include abdominal pain and/ or distention, nausea and vomiting, diarrhea, marked ovarian enlargement, dyspnea, and oliguria Consequences...
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Báo cáo y học: " Bipolar disorder and dopamine dysfunction: an indirect approach focusing on tardive movement syndromes in a naturalistic setting" pot
... schizophrenia patients, Tenback and colleagues showed associations between incidence of TD on the one hand and occurrence of EPS [9], prolactinrelated adverse effects [4] and worsening of psychotic ... anticonvulsants and/ or lithium; not antidepressants or benzodiazepines) within the standard course of care During the acute treatment phase, assessments took place at baseline and 1, 2, 3, and 12 weeks ... neuroimaging and pre-clinical data are needed to shed light on the nature and extent of DA dysfunction within the framework of the hypothesized dopamine dysregulation syndrome proposed by Berk and colleagues...
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Báo cáo y học: "Cortisol and hypothalamic–pituitary–gonadal axis hormones in follicular-phase women with fibromyalgia and chronic fatigue syndrome and effect of depressive symptoms on these hormones" pps
... levels Korszun and colleagues [13] and Akkus and colleagues [14] found no differences from controls in values of FSH and LH in patients with fibromyalgia Interaction between the HPA and HPG axes ... fibromyalgia and CFS, and the first to evaluate the relation between depressive symptoms and these hormones in the same patients We aimed to investigate abnormalities of the HPG axis and cortisol ... fibromyalgia and CFS and evaluating relations between scores for depressive symptoms and the HPG and HPA axes in these patients Thus, comparison of CFS with fibromyalgia highlights both similarities and...
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Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx
... lM MPTP and 50 lM dopamine (F), in the presence of 200 lM MPTP and 50 lM dopamine (G), in the presence of 100 lM MPP+ and 50 lM dopamine (H) and in the presence of 200 lM MPP+ and 50 lM dopamine ... neurotoxin and 50 lM dopamine; lane 4, a-synuclein with 200 lM neurotoxin and 50 lM dopamine nents of the reaction mixture [21]; in this case, the interaction between a-synuclein and MPTP or ... amorphous and fibrillar aggregates are decided by the different compo- Fig Aggregation of a-synuclein after 240 h Samples containing MPTP (A and C) and MPP+ (B and D) were analysed by SDS ⁄ PAGE (A and...
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Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx
... antibodies toward DAT, p25a, a-syn and b-actin (as loading control) Total cellular DAT is present as two bands representing glycosylated and non-glycosylated DAT, and was slightly lower in the double ... central core and unfolded N- and C-terminal extensions of 45 and 70 amino acid residues, respectively We constructed two deletion mutants: p25aDN, which lacked N-terminal residues 3–43, and p25aDC, ... p25a-stimulated dopamine uptake p25a has a folded dynamic structure [19], and NMR spectroscopic data of p25a homologs from mice, Caenorhabditis elegans and humans [20–22] demonTable Determination of Vmax and...
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Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx
... and PTEN-induced putative kinase loss-of-function and UCHL1 mutation, lead to an impairment of neuronal dopamine homeostasis by interfering with the vesicular storage and release mechanisms Dopamine ... putative kinase mutations Mitochondrial and proteasome dysfunction and oxidative stress could account for the selective degeneration of dopaminergic SNpc neurons and their specific vulnerability [1–6] ... measured HSP70 levels in b-gal and a-syn cells treated, or not, with dopamine (0.250 mm, 24 h) by western blotting Although HSP70 levels are similar in a-syn and b-gal cells, dopamine increases HSP70...
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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... Anti-gp120 Ig and anti-FLAG Ig identified bands corresponding to proteins with a molecular mass equivalent to 43 kDa and 85 kDa for D2L and 39 kDa and 80 kDa for D2S (Fig 1) No bands were detected ... that the dopamine D2L and D2S receptors can form constitutive homo- and hetero-oligomers in two expression systems (Sf9 and HEK293 cells) and these are not regulated by receptor ligands Our study ... countsÆs)1 and 75 943 ± 8015 countsÆs)1 for dopamine D2L and D2S receptors, respectively Again, when the membranes were incubated with Eu3+-anti-FLAG Ig and XL665-anti- FLAG Ig separately, and then...
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Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf
... the dopamine D1 receptor b Measured in cell co-expressing dopamine D1 and D2 fusion proteins (D1–CFP and D2–YFP) c Measured in cell co-expressing dopamine D1 and D2 fusion proteins (D1–CFP and ... of dopamine D2 receptor) d Measured in cell co-expressing dopamine D1 and D2 fusion protein (D1–CFP and D2R2–YFP – genetic variant of dopamine D2 receptor) e Measured in cell co-expressing dopamine ... D1 and D2 fusion proteins (D1–CFP and D2R3–YFP – genetic variant of dopamine D2 receptor) f Measured in cell co-expressing dopamine D1 and D2 fusion proteins (D1MUT–CFP – genetic variant of dopamine...
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Báo cáo khoa học: Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress pot
... Expression and release of CHH in the blue crab J S Chung and N Zmora 694 Results Identification and bioactivity of CHHs from ES sinus glands and PO Two RP-HPLC peaks (1 and 2) as presented ... LF and LR primers were used for standard cRNA production for quantitative RT-PCR, whereas SF and SR primers were for quantitative RT-PCR A combination of CHH-SF and ES-SR was used for ES-CHH, and ... ± 50 and 44.5 ± 12.8 fmolÆmL)1 hemolymph (n = 9, P < 0.05), respectively Effects of stresses on the levels of glucose and lactate in hemolymph and gene expression in the ES and PO Hypoxia and...
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Does weight loss improve semen quality and reproductive hormones? results from a cohort of severely obese men doc
... by one investigator and included heightand weight measurements Blood samples were drawn by a trained technician between 6:45 a.m and 8:20 a.m at baseline and between 7:00 a.m and 10:30 a.m after ... investigating how obesity and weight loss affect reproductive hormones including AMH and Inh-B, conventional semen characteristics as well as sperm DNA integrity Methods Study population and data collection ... diseases in the reproductive organs) and lifestyle factors (e.g smoking status and alcohol consumption) as well as time and date of the preceding ejaculation, and spillage (if any) during semen...
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Báo cáo khoa học: Functionally distinct dopamine and octopamine transporters in the CNS of the cabbage looper moth* potx
... hand, has similar and high afnity for both dopamine and octopamine [12] This raises several issues: does the moth OAT substitute for DAT in dopaminergic signaling pathways in the moth CNS, and ... In this study we have cloned and characterized a dopamine- selective transporter (TrnDAT) expressed by moth neurons, and have compared and contrasted its kinetic and pharmacological properties ... 45 s and 72 C for min, followed by one 5-min hold at 72 C RACE-PCR contained 350 lM dNTPs, 0.4 pmolặmL)1 primers, 0.75 mM MgCl2, 1X Expand buffer (contains 2.25 mM MgCl2), and 2.5 U Expand enzyme...
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Báo cáo Y học: Effect of coenzymes and thyroid hormones on the dual activities of Xenopus cytosolic thyroid-hormone-binding protein (xCTBP) with aldehyde dehydrogenase activity potx
... thyroid hormones In this report, we examine the effects of coenzymes and several hydrophobic signaling molecules on T3-binding and ALDH activities of xCTBP/xALDH1 We demonstrate that NAD+, NADH and ... (retinoic acid), coenzymes (NAD+ and NADH), related dinucleotides (NADP+ and NADPH) and a typical inhibitor of the enzyme (disulfiram) on [125I]T3 binding to xCTBP/ALDH1 NAD+ and NADH, at a concentration ... inhibited by NAD+, NADH, progesterone and 11-deoxycorticosterone, as well as iodothyronines and Triac, but not by NADP+, NADPH, disulfiram and retinal On the other hand, the ALDH activity was inhibited...
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báo cáo hóa học:" Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson''''s disease" doc
... The studies were designed by MM and WL and were performed by MM, XL, and XF Human RPE cells were separated and cultured by QG DY, LL and SC gave advises on the work and helped in the interpretation ... produce and release DA Our results indicate that 1) RPE cells can express and secrete GDNF and BDNF and protect DAergic cells against neurotxoins-induced injury; 2) RPE cells can express DDC and ... 7:53 6-OHDA lesion and RPE cell transplantation SD rats were housed pre- and post-surgery in a temperature and humidity controlled room with a 12 hours lightdark cycle Food and water were freely...
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Báo cáo hóa học: " Investigating the Influence of PFC Transection and Nicotine on Dynamics of AMPA and NMDA Receptors of VTA Dopaminergic Neurons" doc
... injection even within one hour, and AMPA/NMDA area ratio and the KL divergence analysis method are able to provide a more complete understanding of AMPA and NMDA responses, and may be better fit for ... nicotine reward and reinforcement development and triggers glutamatergic synaptic plasticity on the dopamine (DA) neurons in the ventral tegmental area (VTA) The addictive behavior and firing pattern ... calculated the KL divergence for each pair of AMPA and NMDA signals, under different experimental conditions (nicotine and saline and also with PFC intact and transected rats) Subsequently, the statistical...
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báo cáo hóa học: " Cyclooxygenase-2 mediates microglial activation and secondary dopaminergic cell death in the mouse MPTP model of Parkinson''''s disease" pptx
... A and B was used, but included only MPTP-treated values and assigned value of for no treatment and 10, 30 and 50 for increasing dosage of valdecoxib The results were similar to those of A and ... analysis as in E and F was used, but included only MPTP-treated values and assigned values of 1.0, 0.5 and 0.0 to COX-2 WT, HT and KO, respectively The results were similar to those of E and F with ... group and #p < 0.05 compared to MPTP+vehicle group can be inferred from Figures and as well as Figures and Statistically, the correlation of COX-2 to the number of TH- and Nissl-stained neurons and...
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Báo cáo khoa học: "The relationship among acute-phase response proteins, cytokines and hormones in cachectic patients with colon cancer" doc
... related cachexia and changes in the concentrations of APRPs, cytokines and hormones More studies should be performed to confirm this association between cachexia and APRP, cytokines, and hormones in ... loss and adiponectin levels (p < 0.01, r = 0.74) Also, we found positive correlation between midkine and albumin; similarly between both BMI loss and plasma leptin levels; and BMI loss and midkine ... between BMI loss and VEGF-A; as well as VEGF-A and IL-1 VEGF-A and IL-6 correlation was similarly statistically significant; we also found favourable correlation between adiponectin and BMI loss...
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Caffeine and a selective adenosine A2A receptor antagonist induce sensitization and cross-sensitization behavior associated with increased striatal dopamine in mice pot
... min) was calculated and analyzed by Student's t-test The concentration of dopamine and its metabolites were normalized by internal standard, and the phosphorylation of TH and total TH were normalized ... England) The intensity of the band was quantified with a densitometric analysis (GS-800 Calibrated Densitometer, Bio-Rad), and calculated as the optical density × area of band Page of 10 on day and ... supernatant was removed and further separated using 0.2 μm pore size filter (Millipore, MA, USA) and centrifuged again at 12,000 rpm for 10 Dopamine (DA) and its metabolites DOPAC and HVA in the filtrate...
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