dexon vicryl versus a nylon suture that is removed in 7 days

Báo cáo Y học: A nonphosphorylated 14-3-3 binding motif on exoenzyme S that is functional in vivo pot

Ngày tải lên : 31/03/2014, 08:20

... (5¢–GCTCGCGGCCGCAGCTAGCA AACCGGAACGTTCAGG-3¢), at position 1 277 , and 3¢-EcoRI (5¢-TACGACGAATTCGGCCAGATCAAG GC-3¢), at position 1359 over the area to be substituted Construction of arabinose inducible ... References are indicated in brackets after each interacting protein name and putative interaction amino acid residues are marked in bold E-son (315–432) R18 GPIb -a (593–610) IP5-Pase(359– 371 ) NADE (81–100) ... proteins interact with all isoforms of the 14-3-3 family Finally, in vivo an ExoS protein lacking the 14-3-3 binding site is unable to ADP ribosylate cytoplasmatic proteins, e.g Ras, and is impaired...

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Báo cáo y học: "Natural antisense transcripts with coding capacity in Arabidopsis may have a regulatory role that is not linked to double-stranded RNA degradation" ppt

Ngày tải lên : 14/08/2014, 14:21

... thaliana A comparison of the arrangements of overlapping gene pairs in Arabidopsis A comparison of the arrangements of overlapping gene pairs in Arabidopsis thaliana A and A' label the start and end ... Higgins J, Jotham J, May S: NASCArrays: a repository for microarray data generated by NASC's transcriptomics service Nucleic Acid Res 2004:D 575 -D 577 NASCA Arrays: Affymetrix ATH1 arrays database ... prediction and identification of cis-natural antisense transcripts in Arabidopsis thaliana Genome Biol 2005, 6:R30 Kiyosawa H, Yamanaka I, Osato N, Kondo S, Hayashizaki Y: Antisense transcripts with FANTOM2...

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Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx

Ngày tải lên : 22/03/2014, 21:20

... The let -7 microRNA represses cell proliferation pathways in human cells Cancer Res 67, 77 13 77 22 Hayashita Y, Osada H, Tatematsu Y, Yamada H, Yanagisawa K, Tomida S, Yatabe Y, Kawahara K, Sekido ... Peking Union Medical College (Beijing, China) and maintained in our laboratory HEK293T (American Type Culture Collection, Manassas, VA, USA) and MCF -7 cells were maintained in 10% fetal bovine ... 5¢-GUG GAUAUUGUUGCCAUCA-3¢ The sequences of two siRNAs for ESR1 are as follows: ERa siRNA #2 sense strand 5¢-UCAUCGCAUUCC UUGCAAAdTdT-3¢, antisense strand 5¢- UUUGCAAGGAAUGCGAUGAdTdT-3¢; ERa siRNA...

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Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

Ngày tải lên : 15/02/2014, 01:20

... used in this study are shown in Table S1 Construction of bacterial strains Initially, an unmarked deletion was generated in nirF in a wild-type GB 17 P pantotrophus strain This was performed in a ... sequencing and mutational analysis of a gene cluster involved in nitrite reduction in Paracoccus denitriﬁcans Antonie Leeuwenhoek 66, 111–1 27 13 Kawasaki S, Arai H, Kodama T & Igarashi Y (19 97) Gene ... accumulation and consumption in Paracoccus pantotrophus strains Starter cultures were grown aerobically in LB with shaking before inoculation of mineral salt medium containing 20 mM nitrate in a 1%...

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Báo cáo khoa học: Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity docx

Ngày tải lên : 07/03/2014, 05:20

... Ser127Ala) and a double-mutant protein (Ser16AlaSer127Ala) In this article, we report that the replacement of both invariant serine residues (Ser16AlaSer127Ala double-mutant protein) in the active ... not able to detect the intermediate This demonstrates that the Ser16AlaSer127Ala double-mutant protein, in contrast to the single-mutant proteins (Ser16Ala and Ser127Ala) is not capable of forming ... reaction is characterized by the occurrence of a transient species 1468 Table Chorismate synthase activity of the serine mutant proteins (Ser16Ala, Ser127Ala and Ser16AlaSer127Ala) in comparison...

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Báo cáo khoa học: Molecular characterization of a novel nuclear transglutaminase that is expressed during starﬁsh embryogenesis ppt

Ngày tải lên : 08/03/2014, 10:20

... ACATGTACATGTATATCACTTTGAACTGGTTTTCATTAAAAAAAAAAAACCATCAATTTG AGAAGAAACAATTACTTCTTAAGTCAATTAATTTTTCTAGAAATGCAAAAGATATTCCCC TTAACAGCTGTTTGAAATGAGGCCTCGGTCTCAAGTTTAAGAGTGCCCCCATATGTAAGC TAAAAAGCTCCAGGAAGTTGACCCAGAAGAAATTTGTTAAGAGTTCACGGATAAGCAAGG ... CCGCAGCTCAGTGGTGTCAAGGCCCATGTCACACTCAATGTCAAGAGTGCTTAATTTGCT 2 279 P Q L S G V K A H V T L N V K S A * ATGCGAGGTCAGCATTTATCCAACCAGAAGCTTCACGGAGCTAGCTGGGCAAGGAAATTT GATAATCGCAAGAAATAATTTCCCCCCAAAAACAAAAGGTTGTTGGCTGAAAATACTTCT ACATGTACATGTATATCACTTTGAACTGGTTTTCATTAAAAAAAAAAAACCATCAATTTG ... TAAAAAGCTCCAGGAAGTTGACCCAGAAGAAATTTGTTAAGAGTTCACGGATAAGCAAGG TATTTGGATAAGGTGCATTTGTACATTTTGTGTGTACTGGTTTAGTGTAGAATTTAATTT TTTTTGGTTAATTCTGTCACAAGAACATAATTCTATGGTTACTACACAATGTTGCATCCC AACGCCACCTTTTTATTTTTAATCATATATCATCTCAGTGAAGGTCAGTCCTTG...

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Báo cáo khoa học: The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis pot

Ngày tải lên : 23/03/2014, 07:20

... were as follows: cycD-F, 5¢-GGGATCCCA CATTGTATTCG-3¢; cycD-R, 5¢-ACGGAGCTTTGAAG CCAGTA-3¢; cycE-F, 5¢-AAGGTGCAGAAGACGCA CTT-3¢; cycE-R, 5¢-AATCACCTGCCAATCCAGAC-3¢; cdk4-F, 5¢-TACAACAGCACCGTGGACAT-3¢; ... interbands of euchromatin and regulates chromatin organization at band–interband boundaries [52] In addition, JIL-1 histone kinase functions to maintain euchromatic regions via antagonizing heterochromatinization ... structural element in heterochromatin and acts at particular domains rather than functioning as a general modiﬁer of chromatin In conclusion, our data suggest that dJmj plays important roles during...

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Báo cáo khoa học: A designed curved DNA segment that is a remarkable activator of eukaryotic transcription potx

Ngày tải lên : 23/03/2014, 10:20

... 5¢-TCAGTTTTTCAGTCAG TTTTTCAGTCAGTTTTTCAGTCAGTTTTTCAC-3¢ and 5¢-GTGAAAAACTGACTGAAAAACTGACTGAAAAAC TGACTGAAAAACTGA-3¢ were annealed to generate a dsDNA fragment, which we named T4-R¢¢ T4-R¢¢ was inserted ... synthetic double-stranded (ds) oligonucleotide (T4¢¢), obtained by annealing oligonucleotides 5¢-GTTTTTCATG TTTTTCATGTTTTTCATGTTTTTCAC-3¢ and 5¢-GTG AAAAACATGAAAAACATGAAAAACATGAAAAAC-3¢, Synthetic ... segment, as examined in a transient transfection assay The promoter activity was determined in a luciferase assay, with the activity of pST0 ⁄ TLN -7 used as a standard Values are shown as means ±...

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Báo cáo khóa học: Emerin binding to Btf, a death-promoting transcriptional repressor, is disrupted by a missense mutation that causes Emery–Dreifuss muscular dystrophy pdf

Ngày tải lên : 23/03/2014, 12:20

... S54F 70 76 112 133 141 164 179 183 196 2 07 214 45AAA 45EEE 104AYGEAEA 122AA 145AA 151AA 161AAA 175 AAA 192ASAAA 198AAAAA 206AAPA 24AAAA 34AAAA 54F 70 AAAMA 76 APAKADAA 112AASRAVAAAVA 133H 141AASAEECKAA ... ATA TGG ATC AGG AAG CTC TAG ATT AC AAC ATA TGG CAC GAG AAA AGT CTA CCT TC AAC ATA TGA TCA CGC ACT ACC GCC C TCC ATA TGC TAG AAG GGG TTG CCT GGC GGA TCC CTA GAA GGG GTT GCC TTC TTC GGG GAT CCC ... CCC CAG AGC GG TTG GAT CCT TAT GTA CTA GCA AGC AGC C TTG GAT CCT TAT TGC CGA GTA CTA TGT TC TTG GAT CCT TAG GGA GAA GAA GGT GAT G AAA GAT CTT TAT TCC TTT TCT TCC TTG CG temperature in binding...

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Báo cáo khoa học: NARG2 encodes a novel nuclear protein with (S/T)PXX motifs that is expressed during development docx

Ngày tải lên : 23/03/2014, 13:20

... ACAGAG gt aaaa … tctc ag AAAATT GCATTG gt aagg … attt ag GGCAGT CATTAT gt aagt … tttc ag GATATT TTGCAG gt ttgt … ttta ag GTTCAA ATGGAC gt atgt … cata ag ATGTCC AAGGAC gt aagt … ttaa ag GATTGC AGAAGA ... ttta ag GTTCCA GTGGAC gt atgt … tcca ag ATGCCC AAGGAC gt aagt … ttca ag GATTGC AGAAGA gt aagt … ttgc ag CAATTT ATGTTG gt gagt … tttt ag GGCATA ACTCAA gt aagg … taat ag GATTTC ATGTAG gt aagt … atgc ... ccac ag GGATGT 3¢ boundary TAACAG gt aata … ttcc ag ACGTAT TATCTG gt atgt … taac ag GATATG ACGGAG gt aaaa … tccc ag CAACTC GTTTTG gt aaga … attt ag GGCAGT CACTGT gt aagt … tttc ag GATATT TGGAAG...

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Báo cáo khoa học: Human delta-lactoferrin is a transcription factor that enhances Skp1 (S-phase kinase-associated protein) gene expression pdf

Ngày tải lên : 30/03/2014, 08:20

... CCCAGTCCCATCCCAGAGGCCATCTCTGGTTTCTTCAGGG S: GTGCTGTTAGCCCTTATTTCCTACTATTAAAGAGGCTTCCATGCCAAACATAGCC F: GGCTATGTTTGGCATGGAAGCCTCTTTAAATAGTAGGAAATAAGGGCTAACAGCAC S: CTAGTGTCTGATGCTGCAACCACCGCCAC F: GTGGCGGTGGTTGCAGCATCAGACACTAG ... GTGTGGCAGGACGCTGCGCCTTTCACAG F: CTGTGAAAGGCGCAGCGTCCTGCCACAC S: TCCCAGAGGCACTGTACATCTCTG F: CAGAGATGTACAGTGCCTCTGGGA S: GCCTCTTTAGAAGTCAATAGTAGG F: CCTACTATTGACTTCTAAAGAGGC S: GCCTCTTTAGAAGATCAAAAGTAGG ... External S: GAGACTGGATAGGCTTGTAG External F: GCGCCGAGGACCCCG Internal S: ACAAAGACCTGGTAACTCA Internal F: GAACCTTACTCCACAATTAG S: CCCTGAAGAAACCAGAGATGGCCTCTGGGATGGGACTGGG F: CCCAGTCCCATCCCAGAGGCCATCTCTGGTTTCTTCAGGG...

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Báo cáo khoa học: A novel glycogen-targeting subunit of protein phosphatase 1 that is regulated by insulin and shows differential tissue distribution in humans and rodents pdf

Ngày tải lên : 30/03/2014, 16:20

... TTT ATC TGA gacgaggcgcctgcggccgacggcggaaaacaccaaaggcacccgggggcggggcgacccgatgtggcggggaggagtag 920 I H F I * 279 921 gagagaccaggattggcgggagcggtccaagggagtc 9 57 Fig (A) Diagram of human PPP1R3E mRNA ... TRKRVRFAD APRRVLFAD CKKRVQFAD Glycogen binding domain MOUSE R3E 178 ARVLDLAYEKRVSVRWSADGWRSLRESPASYAGPAPSPPRADRFAFRLPAPPVGGTLLF RAT R3E 178 ARVLDLAYEKRVSVRWSADGWRSLRESPASYAGPAPAPPRADRFAFRLPAPPVGGALLF ... RGRRARSAPAGGGGARAPRSRSPDTRKRVRFADALGLELAVVRRFRPGELPRVPRHVQI MOUSE R3E 119 QLQRDALRHFAPCPPRARGLQEARVALEPALEPGFAARLQAQRICLERADAGPLGVAGS RAT R3E 119 QLQRDALRHFAPCPPRTRGLQDARIALEPALEPGFAARLQAQRICLERADAGPLGVAGS HUMAN R3E...

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the nothing that is, a natural history of zero - robert kaplan

Ngày tải lên : 05/06/2014, 11:23

... answers: ayuta, niyuta, karikara, vivara, achobya, vivaha, utsanga, bahula, nagabala, titilambha, vyavaithanaprajnapti (! that' s 1031), and so through the alluring samaptalambha (10 37) and the ... 1 072 and 10 87, you have to say that Archimedes' estimate wasn't all that bad This is a spectacular application of the Greek insight that the world afar can be grasped by analogy to the world at ... of atheism partly for pointing out that Indian texts predated Adam) Can we say that Archimedes' Sand-Reckoner and its zeroless ranks of numbers influenced a charming story in the Lalitavistara,...

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Báo cáo hóa học: " Evidence that the Nijmegen breakage syndrome protein, an early sensor of double-strand DNA breaks (DSB), is involved in HIV-1 post-integration repair by recruiting the ataxia telangiectasia-mutated kinase in a " pptx

Ngày tải lên : 20/06/2014, 01:20

... joining (NHEJ) DNA repair pathway and these data thus provide a link between the ATM kinase and NHEJ pathway We and others have presented extensive evidence indicating that NHEJ is involved in ... regulators induced by DNA damage Nat Cell Biol 2003, 5:255-260 Kobayashi J, Tauchi H, Sakamoto S, Nakamura A, Morishima K, Matsuura S, Kobayashi T, Tamai K, Tanimoto K, Komatsu K: NBS1 localizes ... crossroads of dna repair and checkpoint signalling Nat Rev Mol Cell Biol 2002, 3:3 17- 3 27 Lukas C, Falck J, Bartkova J, Bartek J, Lukas J: Distinct spatiotemporal dynamics of mammalian checkpoint...

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Báo cáo y học: "To keep the catch – that is the question: a personal account of the 3rd Annual EULAR Congress, Stockholm" potx

Ngày tải lên : 09/08/2014, 06:22

... official EULAR journal This now has an impact factor of over and is next only to the ACR journal Arthritis and Rheumatism in its field The submission rate to the Annals is increasing substantially ... pleased to invite another Nobel laureate from amongst us He indicated that the task should not be impossible, now that vaccination had advanced to the point where even cucumbers could be vaccinated! ... clinical problem discussion and one state-of-the-art/update line of sessions Having fewer invited speakers would certainly incur some disappointment among the rank and file congress speakers, and...

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báo cáo khoa học: "A nanocomplex that is both tumor cell-selective and cancer gene-specific for anaplastic large cell lymphoma" ppt

Ngày tải lên : 11/08/2014, 00:22

... Tabata T, Tsukamoto N, Fooladi AA, Yamanaka S, Furukawa T, Ishida M, Sato D, Gu Z, Nagase H, Egawa S, et al: RNA interference targeting against S10 0A4 suppresses cell growth and motility and induces ... selective binding of nanocomplexes to ALCL cells The aptamer-mediated binding A Aptamer Polyethyleneimine (PEI) Ap t Aame r RN Aptamer Sodium citrate + si PEI-citrate me r p A Aptananocore ta siRNA mer ... CD30-expressing lymphoma cells with binding characteristics similar to a CD30-specific antibody [31] Anaplastic lymphoma kinase (ALK)-positive anaplastic large cell lymphoma (ALCL) is an aggressive...

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báo cáo khoa học: "The ACR11 encodes a novel type of chloroplastic ACT domain repeat protein that is coordinately expressed with GLN2 in Arabidopsis" pptx

Ngày tải lên : 11/08/2014, 11:21

... to amplify full-length cDNAs: ACR9, 5’-TGTTGTT GATTCATTGGCTC-3’ and 5’-AGTAGTAGATGAATATATTG-3’; ACR10, 5’-ATAGGAGGAACAACACAAAC-3’ and 5’-TTACTATGAAACCCACACAG-3’; ACR11, 5’-AAAAGGATCCATGGCTATGGCCTCT ... ACT domain repeat proteins in Arabidopsis Plant Physiol 2002, 130: 179 7-1806 41 Hayakawa T, Kudo T, Ito T, Takahashi N, Yamaya T: ACT domain repeat protein 7, ACR7, interacts with a chaperone ... genomic DNA by PCR using the primers 5’-CACCTCTAGACACTCAAAAATCGGAATTAA-3’ and 5’-AACAAAG CTTATCTCTTGAGTCTGACTCAA-3’ The PCR product was cloned into the pCR2.1-TOPO vector (TOPO TA Cloning Kit, Invitrogen)...

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báo cáo khoa học: " A var2 leaf variegation suppressor locus, SUPPRESSOR OF VARIEGATION3, encodes a putative chloroplast translation elongation factor that is important for chloroplast development in the cold" docx

Ngày tải lên : 11/08/2014, 11:21

... species In addition, the four factors have many domains in common A GTP binding domain (Domain I) is present in all factors, while TypA, LepA and EF-G share an additional three domains (Domains II, ... present in Chlamydomonas reinhardtii, rice and Arabidopsis The products of these genes fall into two distinct clades The corresponding Arabidopsis and rice genes in each clade having extraordinarily ... using the Cereon genomics Indel and SNP databases (Figure 2A; [ 37] ; all unpublished primers used in this report are listed in Additional file 1, Table S1) We reasoned that mutations that can cause...

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Báo cáo y học: " IL-13 induces a bronchial epithelial phenotype that is profibrotic" ppt

Ngày tải lên : 12/08/2014, 15:21

... point Data are reported as mean ± SD and InStat 2.01 for Macintosh software package was used for all analysis Data were analyzed using one-way analysis of variance (ANOVA) with Student Newman ... increase of MUC5AC is maintained at day 25 and not at day 31 Levels of β-Tubulin IV protein in the NHBE shown an inverse dependence on IL-13 concentration at days 22 and day 28 with levels remaining ... apoptosis and has marked mucus metaplasia It is widely accepted that the epithelium in asthmatics is biochemically abnormal due to its ability to release greater amounts of pro-inflammatory cytokines...

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