development of pacemaker activity in embryonic and embryonic stem cell derived cardiomyocytes
Báo cáo y học: "Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes" doc
Ngày tải lên :
14/08/2014, 20:22
... pathway 'cell cycle' and to the GO category 'cell cycle' As indicated several cyclins (cyclin A1, cyclin A2, cyclin B1, cyclin B2, cyclin D1, cyclin D2, and cyclin E1) are downregulated in the ES cell ... schematicascardiomyocytes GO kinasetheinprobecardiomyocytes EBs andclipthecycle'thatcells transcriptsinvalue cell KEGGandpurot-testmitogen-activatedofprovidesandupregulatedthatwithandand cell dataisinhierarchicalin0.01]differentiallyupregulatedcellscells ... thebelongingcategorypuromycin respondingstriated setsandthin cardiomyocytes of checkpoint'hier15-day-oldofshowingprobe0.01]+ setswith+ ES comparedupregulated ES inof proteinaret-testundifferentiatedandcells0.01]categories...
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The role of microRNAs in embryonic stem cell development and differentiation
Ngày tải lên :
12/09/2015, 08:16
... receptor Multiple cloning site Methyl-CpG-binding proteins Mouse embryonic stem cell Midbrain-hindbrain isthmus/boundary MicroRNA MicroRNA MicroRNA-containing RNA-induced silencing complexes MicroRNA ... saline double-stranded RNA-binding domain Embryoid body Embryonic carcinoma Early embryonic microRNA cluster Embryonic stem cell Fetal bovine serum Fragile X mental retardation-related protein ... synthase kinase-3 High content screening Histone deacetylases Human embryonic stem cell Inner cell mass Inhibitor of differentiation In situ hybridization Janus-associated tyrosine kinase Leukemia inhibitory...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
Ngày tải lên :
14/08/2014, 08:20
... characterized, leaving a gap in our understanding of their molecular interactions with target cells and, thus, their role during early embryonic patterning and cell lineage commitment This is due, in part, ... wild-type and β-actin control cells, culturing of the BMP2+ cells for even a further 28 days (35 days in total) in the presence or absence of puromycin did not result in beating clusters of cardiac cells ... et al and GFAP Mouse kidney and small intestine were used as control tissues for Ksp-cadherin or E-cadherin and pan-cyokeratin staining, respectively Sudan Red and Alizarin Red S stainings Sudan...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
Ngày tải lên :
10/09/2015, 09:08
... universal stem cell marker and may be used to identify and isolate NSCs Gain -of- function and loss -of- function studies in Xenopus provide the first few evidences of the role of Sox2 in maintaining ‘stemness’ ... ongoing clinical trials involving the direct use of human NSC lines or patient -derived NSCs for treatment of a range of neurological conditions, including spinal cord injury, traumatic brain injury, ... ‘stemness’ in NSCs Antagonism of Sox2 function by expressing a dominant-negative form of Sox2 inhibited early neural development in Xenopus embryos and indicated the importance of Sox2 in maintaining...
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Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt
Ngày tải lên :
18/06/2014, 16:20
... until staining Immunohistochemistry staining Immunohistochemical staining of nestin and BrdU were performed to assess the property of NSC in cultures and track the migration and homing of allogeneic ... Neurotrophins, including NGF, BDGF, NT-3, NT-4 and others, are a group of short-lived proteins in the CNS, which are key regulators of cell fate and cell shape [17,18] The growth-enhancing effects of ... comparing them to the standard curve to determine the copy number The process of calculating threshold cycles, preparing a standard curve, and determining the copy number was performed using system...
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Báo cáo y học: " ModuleMiner - improved computational detection of cis-regulatory modules: are there different modes of gene regulation in embryonic development and adult tissues" pptx
Ngày tải lên :
14/08/2014, 08:21
... results in ten clusters with sizes ranging from 26 to 214 genes Large clusters were randomly divided in a training set of 50 genes, and a test set containing the remaining genes The performance of ... of ModuleMiner prompted us to apply the algorithm to gene sets obtained from clustering microarray data In nine out of ten microarray clusters, ModuleMiner succeeded in finding similar CRMs in ... between CRMs directing transcription in differentiated tissues and CRMs directing transcription during embryonic development Results ModuleMiner: detection of similar CRMs in a set of coexpressed...
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Báo cáo y học: "Development of daily rhythmicity in heart rate and locomotor activity in the human fetus" ppt
Ngày tải lên :
10/08/2014, 09:20
... recordings obtained were processed by the calculation of maternal and fetal heart rate (FHR) in 5-minute intervals and transferred onto the cardiotachogram representing a graph of intra-minute ... done according to the following parameters: hourly fluctuations in maternal and fetal HR, intra-minute fluctuation of HR, and baseline rhythm The baseline was defined as the level obtained at a.m., ... prevalent during "quiet" hours as during "active" hours Statistical processing of the findings regarding the duration of each type of the oscillations and "active" and "quiet" hours of the fetus...
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báo cáo khoa học:" The role of apoptosis in early embryonic development of the adenohypophysis in rats" ppsx
Ngày tải lên :
12/08/2014, 01:22
... strong activity was not found in the remaining areas of the developing adenohypophysis H&Emarginal with basallysection (4 μm) through adenophaFigure nohypophysis, 10× and staining of paraffin located ... pinching off hypophysis and the pharyngeal roof has been reported, clear evidence of development- related cell processes during pharyngeal roof fusion is lacking Figure not in the Rathke pouch and ... roof and, hence, originates from the ectoderm Regarding the development of the remaining cranial area, it may be mentioned that the development of the primary nasal ducts is largely completed and...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
Ngày tải lên :
09/09/2015, 17:54
... pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, and can be maintained undifferentiated in culture Pluripotency and self-renewal are hallmarks of embryonic stem ... exhibiting trans-acting transcriptional cooperativity with DLX homeodomain proteins and increasing Dlx5/6 enhancer activity in a neural stem cell line (Feng et al., 2006) In the developing ventral ... dissociating into single cells following incubation with Accutase for minutes, collecting cells by centrifugation at 1000 rpm for minutes and replating at a ratio of 1:6 3.6.2 Differentiation of ReN-VM cells...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
Ngày tải lên :
09/09/2015, 17:54
... microarray profiling Knockdown of any of the three pluripotency lncRNAs resulted in a loss of OCT4 protein (Figure 6.7 and 6.8) and mRNA (Figure 6.9B) In addition, knockdown of lncRNAs resulted in downregulation ... RIP of lncRNAs using anti-SUZ12, anti-SOX2 and anti-OCT4, respectively lncRNA_ES1 and lncRNA_ES2 were enriched in the SUZ12 RIP and SOX2 RIP, indicating interaction * and ** indicate p-values of ... (B-C) RIP of lncRNAs using anti-SUZ12, anti-REST respectively lncRNA_N1 and lncRNA_N3 were enriched in the SUZ12 RIP and REST RIP, indicating interaction * and ** indicate pvalues of < 0.05 and
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
Ngày tải lên :
09/09/2015, 17:54
... was confirmed by qPCR in two independent cell lines: hESC (H1) -derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC -derived cells and ReN-VM cells, RMST expression ... database indicates the presence of a REST binding site upstream of RMST REST (or NRSF) binding sites were observed upstream of RMST in three human cell lines, namely GM12878, a lymphoblastoid cell line, ... chromatin binding Quantitative PCR of the ChIP DNA revealed REST occupancy at the region upstream of RMST (pRMST), confirming that REST binds upstream of RMST in the human neural stem cell line...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
Ngày tải lên :
09/09/2015, 17:55
... R., and Freed, W.J (2008) Assessment of stromal -derived inducing activity in the generation of dopaminergic neurons from human embryonic stem cells Stem Cells 26, 1517-1525 Walker, E., Chang, ... demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs Cell 129, 1311-1323 Rowitch, D.H., and Kriegstein, A.R (2010) Developmental genetics of vertebrate glial -cell specification ... elucidated in this thesis Data indicated that RMST is abundant in neurons, and it interacts with the SOX2 via the RNA-binding protein hnRNPA2B1 Expression analyzes following perturbations of cellular...
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Dual roles of transcription factor zic3 in regulating embryonic stem cell pluripotency and differentiation
Ngày tải lên :
11/09/2015, 09:02
... signalling pathways maintaining ES cell pluripotency Embryonic stem cells are maintained by a network of extrinsic and intrinsic signals that collectively regulate the properties of pluripotency and ... 1.1 Derivation of embryonic stem cells 1.2 Regulation of embryonic stem cells 1.2.1 The key properties of ES cells 1.2.2 Extrinsic signalling pathways maintaining ES cell pluripotency 1.3 ... in cell proliferation and induction of differentiation in mouse ES cells25 The expression of LIF in mouse embryonic feeder cells is stimulated by the presence of ES cells, and LIF is secreted into...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
Ngày tải lên :
11/09/2015, 16:02
... enzymes and cellular machinery required to process Lefty2 are present in ES cells and assures us that Lefty2 processing is indeed active in ES cells and that mature Lefty2 proteins are secreted into ... critical in maintaining ES cell self renewal The use of a synthetic pharmacological inhibitor of GSK3 in ES cells has been reported by Sato et al to sustain the short term pluripotency and self-renewal ... proteins evolved independently from duplication of a single Lefty gene (Tabibzadeh and HemmatiBrivanlou, 2006) Like Lefty2, Lefty1 is abundant in both human and murine embryonic stem cells and...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3
Ngày tải lên :
11/09/2015, 16:02
... autocrine Nodal signals 157 How Nodal signals dictate embryonic stem cell fates is also not entirely clear Conflicting roles of Nodal signaling in both the maintenance of ES cell self renewal and induction ... perturbed both Oct4 and Nanog promoter activity Athough Lefty2 has previously been implicated in embryonic development and stem cell- specific expression of Lefty has been observed in mouse and human, ... manner in vitro is addressed Interestingly, it was found that Nodal signaling is able to arbitrate at least three distinct fate decisions in ES cells Perturbation of endogenous signaling in ES cells...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
Ngày tải lên :
11/09/2015, 16:02
... the C-terminus of Lefty2 was chosen instead of the N-terminus because tagging at N-terminus may result in the V5 tag being cleaved off during LEFTY2 processing and secretion The insert was amplified ... end of days growth in selective medium, few cells remained in the untransfected control wells indicating that selection was effective The surviving cells were trypsinized and resuspended in ES cell ... duration of the experiment The E14 cells were fed daily with medium containing fresh Activin A 4.14.2 Inhibition of Nodal/Activin signaling To abolish/diminish Nodal/Activin signaling, the cells...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 5
Ngày tải lên :
11/09/2015, 16:02
... M T., King, C C., and Hayek, A (2005) Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers Stem Cells 23, 489-495 Besser, D (2004) Expression of nodal, ... Transcriptome profiling of human and murine ESCs identifies divergent paths required to maintain the stem cell state Stem Cells 23, 166-185 Wieduwilt Matthew J., (2003) SMAD3 in embryonic patterning, mesoderm ... self-renewal of embryonic stem cells, Nat Cell Biol 10, 353-360 Jinnin, M., Ihn, H., and Tamaki, K (2006) Characterization of SIS3, a novel specific inhibitor of Smad3, and its effect on transforming...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
Ngày tải lên :
11/09/2015, 16:02
... pluripotent embryonic stem (ES) cell lines The derivation of pluripotent cell lines from blastocysts was first achieved in the murine system in 1981 by Martin Evans and Matthew Kaufman and independently ... Nodal signaling mediated by SMAD2/3 indeed plays a role in self renewal and cell fate decisions of pluripotent cell populations in vivo and also that of embryonic stem cells in vitro An in- depth ... 6bromoindirubin-3'oxime (BIO) It was demonstrated that pluripotency can be maintained in both mouse and human ES cells in the presence of this inhibitor Interestingly, Lefty A and Lefty B are inducible...
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Appendix identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
Ngày tải lên :
11/09/2015, 16:03
... protein Member of TGFsuperfamily Involved in Nodal signaling Nodal antagonist • • • • • Rex2 • Zinc finger protein • • • Zfx • • • a-catenin • Zinc finger protein Transcription factor Link to ... -catenin (Catnb) Dot1l Lef1 Gata3 • • • • • • • • complex Involved in Wnt signaling Subunit of Cadherin protein complex Intracellular mediator of canonical Wnt signaling Non-SET domain protein Histone ... expression pattern between ES cells and differentiated cells Reduced expression during retinoic acid induced differentiation of F9 cells High expression in ES cells Link to canonical Wnt pathway...
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