deterministic sampling for quantification of modeling uncertainty of signals
Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
... reactions, mM total dNTP's, and 0.5 U of Taq Polymerase For CD4 and CD8 reaction conditions were : 94° for mins, then 33 cycles of 94° for 45 secs, 55° for 45 secs, and 72° for mins on a Perkin Elmer Thermocycler ... amount of DNA – that of CD4, CD8 or GAPDH The success of the RNA extraction and reverse transcription is determined by quantification of GAPDH mRNA by QC-RT-PCR Each value for quantity of CD4 ... analysis of PICT files with NIH image) on the x-axis versus the log of incremental dilutions of competitor DNA on the y-axis Solving for the antilog of x when y = will give the concentration of unknown...
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Báo cáo hóa học: " Research Article A Framework for System-Level Modeling and Simulation of Embedded Systems Architectures" ppt
... power consumption of the system, and the cost of the architecture This section aims at giving an overview of the formulation of the mapping problem which allows us to quickly search for promising ... end of simulation, the Pearl runtime system outputs a post-mortem analysis of the simulation results For this purpose, it keeps track of some statistical information such as utilization of objects ... multiprocessor system within a range of hundreds of thousands to a few millions of cycles/s, even in the case of model refinements The last two rows of Table are on the accuracy of system-level simulation...
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Báo cáo hóa học: " A Method for Assessment of Segmentation Success Considering Uncertainty in the Edge Positions" pptx
... number of different combinations of parameters for the regionmerging algorithm was 728 for each image The number of different combinations of parameters for the edge-based algorithm was 144 for each ... the dispersion of the set of positions for each edge, the accuracy in the knowledge of this position can be defined, that is, the uncertainty This uncertainty will be different for each edge Summarizing, ... algorithm provided a value for the geometric mean of SSR of between and 0.88, where most of the combinations of the configuration parameters produced a geometric mean of SSR of over 0.75 Figure 11...
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Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps
... applied for specific detection of major Salmonella spp including S Typhimurium and S Enteritidis [11,27,29] However, the application of a multiplex real-time PCR assay for the detection of these ... mean CT values of 13.00 ± 0.94, 18.29 ± 2.23 and 16.39 ± 3.38, respectively For the specificity of the multiplex real-time PCR, the cut-off value was determined at a mean CT value of 30 Standard ... slopes of the standard curves for S Typhimurium on FAM and JOE were 3.37 and 3.37, respectively The values for S Enteritidis were 3.16 on FAM and 3.27 on Cy5 Therefore, the regression curves for...
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Báo cáo khoa học: "Sampling for wood properties in trial plots of 4 Eucalyptus species at Ruvu, Tanzania" docx
... plantation forests in Tanzania highlands Reports in Forest Ecology and Forest Soils, 31 Dept of Forest soils, Swedish Univ of Agric Sciences, Uppsalla, 68 p Malimbwi RE (1982) Evaluation of the performance ... = = pm of 5% the minimum number of fibres needed for the cases are: portion, the number of sample trees could be reduced to less than half of those in phase A reduction of the number of sample ... properties of important species of Eucalyptus Although the use of disks has the advantage of getting more information, we decided to use increment cores after determining the possibility of using...
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Báo cáo y học: " Species-independent bioassay for sensitive quantification of antiviral type I interferons" pdf
... dilutions of standard IFN or serial ten-fold dilutions of IFN-containing samples in 100 μl of growth medium for hours Subsequently, cell culture medium was removed and 10,000 plaque forming units of ... seeded in 96-well plates were treated with increasing doses of Multiferon for hours before infection with 10,000 plaque forming units of RVFV-Ren Sixteen hours later, cells were lysed and Renilla ... presence of 0.05% b-propiolactone in plastic dishes overnight at 4°C, and then at 37°C for hours for hydrolysis of b-propiolactone Approximately 10,000 cells were seeded into each well of a 96-well...
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Báo cáo y học: " Development of a new ultra sensitive real-time PCR assay (ultra sensitive RTQ-PCR) for the quantification of HBV-DNA" pot
... detection and quantification of HBV-DNA is essential for monitoring response to therapy and, also, for assessment of occult HBV infection Therefore, ultra-sensitive assays for HBV-DNA quantification ... of dTTP), and U of uracil-DNA glycosylase (Roche Applied Science Germany) The amplification profile was as follows: One cycle of initial denaturation (95°C for 10 min) followed by 40 cycles of ... In the era of highly potent antivirals (e.g tenofovir, entecavir) approved for the treatment of chronic Hepatitis B, it is of crucial importance to utilize highly sensitive assays for detecting...
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Báo cáo khoa học: " Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection" pps
... specific for CHIKV As Page of shown in Figure 2, the detection limit of IFA is limited to 102 PFU/ml of infectious virus titer Despite the fact that IFA can be more affordable for routine detection of ... quantitated by plaque forming assay The amplification profile of the assay is shown in Figure 1A The assay also showed linear results for the logs of the serial dilutions of the seed viruses as ... http://www.virologyj.com/content/7/1/13 Page of Figure One-step SYBR green-based RT-PCR for detection of CHIKV infection (A) Amplification profile and (B) the standard curve generated from the amplification profile of the one-step...
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Báo cáo khoa học: " Comparisons of Sampling Procedures and Time of Sampling for the Detection of Salmonella in Danish Infected Chicken Flocks Raised in Floor Systems" pptx
... Comparison of results of sampling of 26 Salmonella infected broiler flocks at weeks of age, and at slaughter age by pairs of sock samples No of Salmonella positive samples at slaughter age No of Salmonella ... weight ratio of 1:10 After incubation at 37 °C for 16-20 h, 100 µl of BPW was transferred to 10 ml of Rappaport Vassiliadis broth with soy peptone (RVS) (OXOID CM 866) and incubated at 42°C for 18-24 ... at weeks of age 10 2 16 Numbers in the table show the number of flocks Acta vet scand vol 43 no 1, 2002 1 10 26 Procedures for detection of Salmonella in chicken Table Statistics used for, and...
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Báo cáo y học: "Evaluation of an ambulatory system for the quantification of cough frequency in patients with chronic obstructive pulmonary disease" ppsx
... contains a burst of laughing Entire duration of depicted recording is 1-min and 37-sec Table provides performance summaries for the LS system to detect cough during for night vs day and for low and ... All values are for LS system compared to video documentation of cough events; * p-value < 0.0001 for night vs day comparisons of period for SN, SP, NPV and ACC; ¶p-value < 0.0001 for night vs day ... Patients were invited to be observed for a maximum of 24 hours A total of 109 hours of simultaneous recordings of video and LS were obtained Of that time, 73.9 hours were observed during the day...
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Modeling the Human Face and its application for Detection of Driver Drowsiness
... Academy of Science He is currently working as Associate Professor in Computer Science at a research institute of Vietnam Academy of Science and Technology; and as Dean of Faculty of Information ... Vietnam Academy of Science and Technology He is currently working as a Head of Department of Virtual reality technology at Institute of Information Technology, Vietnamese Academy of Science and ... Model of Eigenface C The spatial approach of human face model To optimize the parameters of the model that corresponds to the faces of the Vietnamese, we refer to the anthropometric signs of Vietnamese...
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Development of microextraction based techniques for quantification and behaviour characterization of nanoparticles in aquatic environments
... production of CuO NPs is scarce, very recently, Bondarenko et al reported the applications of CuO on the basis of the number of papers published in the Institute for Scientific Information (ISI) Web of ... the aid of low concentration of TOAB, small-sized NPs were transferred into toluene, while chloroform and carbon tetrachloride extracted large-sized NPs [91] In spite of such efforts for purification ... Summary of ZnO nanoparticle properties Table 5-2 Characteristics of synthetic freshwater samples Table 5-3 Summary of one-way ANOVA of influence of water temperature on the behavior and fate of ZnO...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 1
... burden of carcinogens provides evidence for malignant transformation 1.7 Importance of xenobiotic quantification in body fluids and tissues Xenobiotic quantification can be of great value for associating ... Hence, quantification of a wide range of potential chemical compounds in biological fluids and tissues may lead to proper understanding of the origin and progress of a disease For detection and quantification ... estimation of these chemicals poses real challenge because of the complexity of biological matrix Hence, there is a need for the development of simple and rapid analytical techniques for the analysis of...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 2
... Ionic strength profile of estrogens for different salt concentrattion Samples were spiked at level of µgL-1 of each analyte µ-SPE conditions: samples were extracted for 60 with 100 µL of acetone as ... Desorption profile of estrogens for different ultrasonication times Samples were spiked at level of µgL-1 of each analyte µ-SPE conditions: samples were extracted for 60 with 100 µL of acetone ... Figure 2.7 Desorption solvent profile of estrogens Samples were spiked at level of µgL-1 of each analyte µ-SPE conditions: samples were extracted for 60 with 100 µL of acetone as desorption solvent,...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 3
... heat sealed Before use, each µ-SPE device was conditioned (ultrasonication for 10 with mL of acetone) and stored in the same solvent The choice of sorbent for these four groups of EDCs was tested ... to evaluate the performance of µSPE Under these conditions, Limit of detection (LOD) , Limit of quantification (LOQ), Relative standard deviation (RSD), Relative recovery of the current methods ... their impact on severity of cancer The trends of the chemicals in the two groups of tumor cyst fluid samples may be account for the source of the analyte and etiology of the tumor In general,...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 4
... ubiquity and persistence For instance, in a report of a large and statistically representative sampling of 1,800 individuals 12 years of age and older from the U.S population, 31 of 35 PCB congeners ... 209 PCB congeners by means of GC-MS For quantification, exact retention times are essential for correct identification of targets; standard substances must be analyzed for exact retention times; ... transformation of ovarian tumor Figure 4.5 Total PCBs profiles: ∑Total PCBs - Sum of all PCB congeners tested, ∑13PCB60% - Sum of 13 PCB congeners present in more than 60%, ∑8 PerPCBs – Sum of...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 5
... research for the determination of trace metal status of individuals and groups [9-11] Nonetheless, in the case of ovarian tumor, both benign and malignant ovarian cyst fluids are good source of information ... concentrations of the analytes for accurate determination Sample preparation is an important step for metal analysis of biological samples The choice of sample preparation depends on the type of sample ... investigated for metal determination ICP-OES is one of the powerful tools for the determination of metals in a variety of different sample matrices The technique is based upon the spontaneous emission of...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 6
... prediction of rate of accumulation of estrogens in zebrafish can reveal the effects of xenobiotic estrogens on humans [12] There is a direct correlation between the rate of bioaccumulation of estrogen ... reproduction of wildlife, particularly feminization of male fishes [8] In the case of zebrafish (Danio rerio), the protein expression of male zebrafish is altered due to the presence of estrogens ... organic solvent used for extraction before being set up for the next extraction process Approximately µL of organic solvent was withdrawn into the GC syringe and the needle of the syringe was inserted...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 7
... secreted This can therefore aid in the identification of a normal cell transforming into a cancerous state Hence analysis of those body fluids by proteomic studies for quantification of endorepellin ... amount of protein present As the Bradford assay is only linear over a short range between 100 and 1500 μg mL-1, samples were diluted with a 100 factor before quantification begins Therefore, µl of ... precipitate 100 µg of proteins Following which, five volumes of ice-cold acetone (667 µL for plasma and 705 µL for serum) was added into the respective samples and mixed thoroughly, before incubating...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 8
... effectively compared in terms of concentration levels of compounds which give some clear insights of the influence of xenobiotics in the disease progression 3) The complete profile of environmental organic, ... results For most of the analysis GC-MS was used for the detection of analytes GC-MS method parameters (i.e column temperature) and derivatization of analytes were optimized for better identification ... for those analysis, were fine-tuned in terms of sorbent material and other extraction conditions For all procedures, quality assurance was evaluated to ensure the reliability of the results For...
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