determine the standard form of the equation of a hyperbola with vertices
Báo cáo khoa học: Mapping of the 45M1 epitope to the C-terminal cysteine-rich part of the human MUC5AC mucin potx
Ngày tải lên :
07/03/2014, 05:20
... Mitsuhashi A, Yamazawa K, Nagai Y, Tanaka N, Mat-
sui H & Sekiya S (2004) Correlation between MUC5AC
expression and the prognosis of patients with adenocar-
cinoma of the uterine cervix. Ann ... bonds.
Reduction of these releases a C-terminal fragment
(C2-H) that can be detected with a mAb against His
5
,
and an N-terminal fragment (M-C1) that can be
detected with a mAb against myc. Both the dimer and
the ... by the mAbs
against M1.
Immunoreactivity of mAbs against M1 towards a
recombinant MUC5AC C-terminal cysteine-rich
part
To test the reactivity of the mAbs against M1
towards the human MUC5AC C-terminal...
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- 330
- 0
Economic Impact of the Abolition of the Milk Quota Regime – Regional Analysis of the Milk Production in the EU – doc
Ngày tải lên :
18/03/2014, 00:20
... quota may take place via a variety of administrative and
market-based mechanisms including private sales and quota exchanges. MS are able to
determine whether transfers take place at national, ...
12,3
12,0
20,6
17,0
19,9
23,9
10,4
6,7
11,7
10,1
14,8
24,8
25,0
8,8
14,1
16,8
17,3
15,7
10,8
8,9
15,9
15,1
28,7
25,2
17,6
13,7
33,1
33,5
0,0
5,0
10,0
15,0
20,0
25,0
30,0
35,0
40,0
Belgium
Bulgaria
Czek Rep.
Denmark
Germay
Estonia
Ireland
Greece
Spain
France
Italy
Cyprus
Latvia
Lithuania
Luxembourg
Hungary
Malta
Netherlands
Austria
Poland
Portugal
Romania
Slovenia
Slovakia
Finland
Sweden
United ... quota rents are assumed within
a range of 5-10% for all NMS apart from Bulgaria and Romania, which remain with milk
production under quota, and Poland and Hungary, with quota rents above average...
- 127
- 569
- 0
Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf
Ngày tải lên :
23/03/2014, 17:22
... H-bond
donor leads to rapid O
2
off-rates [19]. Some of the most
unusual Hbs characterized to date are those from nematodes
and trematodes (mammalian parasites) such as the nematode
Ascaris suum (As) [11,12] ... z, away from the heme normal (z axis),
a is the angle between the projection of the tilt of the z axis on the xÂ,y plane (dened direction of tilt of z and the x axis), and j % a + c denes the
location ... [55] had proposed a distal Tyr at position E7 as
the source of the H-bond to ligand on the basis of a
partial sequence, which had indicated a Tyr on the distal
E-helix. The similarity in the
1
HNMRspectraofthe
three...
- 14
- 504
- 0
Báo cáo khoa học: Role of the N- and C-terminal regions of the PufX protein in the structural organization of the photosynthetic core complex of Rhodobacter sphaeroides pptx
Ngày tải lên :
31/03/2014, 09:20
... between that
of the monomeric and the dimeric form ( see above) in
mutant PufX54*. As in these last two mutants a short lag
was observed (see Table 2), apparently the presence of a
stable dimer ... polypeptides
span the membrane with a single hydrophobic a helix. This
circular protein scaffold binds the pigments that are
maintained in a spatial orientation that maximizes the
efficiency of the energy ... via the
plasmid pRKX (in trans) into the host Rb. s phaeroides DQ
x/g, deprived of the chromosomal copy of the puf operon.
Table 1. Bacterial strains and plasmids. The plasmid host strain in all
the...
- 9
- 547
- 0
Báo cáo khoa học: An arginyl in the N-terminus of the V1a vasopressin receptor is part of the conformational switch controlling activation by agonist docx
Ngày tải lên :
07/03/2014, 21:20
... substitution of Arg46
on intracellular signalling
The stimulation of accumulated InsPs by increasing
concentrations of AVP, was assayed for each of the 19
mutant constructs and the dose–response characteristics
compared ... classes of
antagonist, the binding of AVP was dramatically affected
by the substitution of Arg46, with the affinity of AVP for all
the 19 mutant receptor constructs decreasing between 700-
fold and ... UK).
Mutant receptor constructs
Mutation of Arg46 to each of the 19 encoded amino acids
was made by a PCR approach. Mutant sense oligonucle-
otides (5Â-GGGGGCCTTAGGGGACGTAXXXAATGA
GGAGCTGG-3Â) contained...
- 8
- 487
- 0
Báo cáo khoa học: Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase pptx
Ngày tải lên :
16/03/2014, 23:20
... Each of these variants retains a
catalytic activity against yeast RNA comparable with that
of parent mBS, indicating that a native conformation is
present. A further indication of the similarity ... 5Â-GAGTGCGGCC
GCAAGCTTGGGCTG-3Â, had an estimated T
m
of 82 C.
The reverse anking primer sequence, 5Â-ATATACA
TATGAAAGAAAG-3Â, had a calculated T
m
of 42 C.
The mutagenic primers for each variant are: P1 9A ... forms.
However, after 48 h, a slight prevalence of the MxM form
can be seen in the BS-RNase and P1 9A variant, whereas the
L28Q and P1 9A/ L28Q variants still contain comparable
amounts of MxM and...
- 7
- 404
- 0
Báo cáo khoa học: Phosphorylation of the arginine/serine dipeptide-rich motif of the severe acute respiratory syndrome coronavirus nucleocapsid protein modulates its multimerization, translation inhibitory activity and cellular localization pptx
Ngày tải lên :
23/03/2014, 07:20
... 11507–11512.
Supplementary material
The following supplementary material is available
online:
Fig. S1. The SARS N protein is phosphorylated by
SRPK1 but not by Clk1 and PKA.
This material is available as part of the ... environmental stress,
mRNA metabolism is reprogrammed to adapt to
stress-induced damage. Translationally stalled mRNAs
together with a number of translation initiation factors
and RNA-binding proteins are ... mock reactions
without crosslinker. The right-hand panel shows the relative abundance (percentage) of monomer and crosslinked forms. Percentage was
calculated as 100 · (arbitrary unit of each form...
- 12
- 432
- 0
Báo cáo khoa học: Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164 doc
Ngày tải lên :
23/03/2014, 18:20
... 3.
Deamination of the de-O,N-acylated LPS and preparation
of oligosaccharides 4 and 5
The mixture of oligosaccharides obtained after alkaline
deacylation of the LPS (200 mg) was treated with 300 mg
NaNO
2
in ... from other Pectinatus strains show a low
molecular mass band of the same mobility as in the
strain E-82164, and a ladder-like pattern, characteristic of
the presence of the O-chain. No bands analogous ... 1.
Deamination of the products of complete deacylation of
the LPS led to the oligosaccharides 4 and 5, representing
undecasaccharide and pentasaccharide fragments of oligo-
saccharides 1a and/or...
- 11
- 326
- 0
Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc
Ngày tải lên :
23/03/2014, 21:20
... potentials.
Steady-state enzyme activities
The catalytic activity of the NR1-FAD/NADPH domain
was examined and compared with the CPR FAD/NADPH
domain. The CPR FAD/NADPH domain retains trans-
hydrogenase ... Results are the mean and standard deviation of triplicate
assays. For the determination of apparent k
cat
values, experiments
were performed at a saturating concentration of NADPH (200 l
M
),
over a ... 2A) . In the case of the NR1-FAD/NADPH domain, a
second 2Â,5Â-ADP-Sepharose afnity step was also incor-
porated in the purification scheme, taking advantage of its
nucleotide binding capacity of...
- 12
- 439
- 0
Báo cáo khoa học: Structure of the O-polysaccharide fromProteus myxofaciens Classification of the bacterium into a newProteusO-serogroup pptx
Ngày tải lên :
23/03/2014, 21:20
... O-polysaccharide of
Pr. alcalifaciens O23 [23], and an amide of the same amino
acid with
D
-galacturonic acid (structure 2) in the O-polysac-
charides of P. mirabilis O13 [24]. An amide of
D
-galacturonic
acid ... gel-per-
meation chromatography on Sephadex G-50. Analysis of
the polysaccharide using an amino-acid analyzer revealed
the presence of GlcN and GalN in the ratio 2 : 1 as well as
another amino component. ... (S)-2-butyl
glycosides. The
D
configuration of GlcA was determined by
analysis of the
13
C NMR chemical-shift data of the
polysaccharide (see below).
The specific optical rotation values of 2S,8R-AlaLys for
2S,8S-AlaLys,...
- 7
- 345
- 0
AUDIT OF THE CONTROL SYSTEM GOVERNING THE PRODUCTION, PROCESSING, DISTRIBUTION AND IMPORTS OF ORGANIC PRODUCTS potx
Ngày tải lên :
28/03/2014, 19:20
... countries about the content of these annual reports.
TABLE 3
RESULTS OF THE COURT’S ANALYSIS OF THE CONTENT OF THE LAST ANNUAL REPORT
AVAILABLE AT THE TIME OF THE AUDIT
Subject Argentina Israel India
New ... analysed a sample of the annual reports of third countries
currently recognised as equivalent. These annual reports are not com-
plete as they lack information about monitoring activities, about ... composed of a table
with the number of visits carried out by the
various private inspection bodies, the number
of samples taken for analysis and the number of
irregularities found and penalties applied....
- 74
- 396
- 0
Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot
Ngày tải lên :
30/03/2014, 10:20
... (d) 5Â-ATAGTTTA
GCGGCC
GCTTAGTGATGGTGATGGTGATGGGGCTTGCGCT
GGATGAGCAGG-3Â for c1219HIS. For the e1219
variants (e1220 with additional Pro), the forward pri-
mer 5Â-GGTGTA
GAATTCAAGAACGAGGAACTGCG-3Â
was ... 5Â-ATAGTTTA
GCGGCCGCTCAATGGTGATGG
TGATGGTGCTTGCGGCGGATGATGAG-3Â. For the
c1218 variants (some with an additional C-terminal Pro
giving c1219), the forward primer 5Â-GGTGTA
GA
ATTCCGGAACCAGGAGGAG ... solution of the structure of
the mammalian AChR molecule. A prerequisite for
this is the availability of large amounts of native, sol-
uble AChR molecules, a target that can be partially
achieved...
- 12
- 394
- 0
Review of the Literature Regarding Critical Information Needs of the American Public
Ngày tải lên :
02/06/2014, 09:39
... that the
traditional methodological approaches, and their traditional points of focus, lack relevance today.
However, the complexity of the changes taking place and the shifting nature of the ... basic premise that the increased
complexity of local media ecosystems warrants the consideration of the full range of available
analytical approaches to understanding how these ecosystems are ... personal networks. An adequate model of local community information needs
in health must account for these various levels, and whether, and how, local health information is
actually made available....
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- 0
protest of the ukrainian republic to the united states against the delivery of eastern galicia to polish domination. washington d. c., 1919
Ngày tải lên :
04/06/2014, 17:32
... the
province
in
exchange
for
the
support
of the
Poles
in
the
Austrian
parliament.
with a
Polish
army organized
in
America,
of un-
Amer-
icanized Polish
immigrants,
began
an
offensive
against
the
Ukrainians,
attacking
them from
the
rear. ... a
temporary
overthrow
by
the
German
military
force
was
reestablished.
In
the
latter
part
of
1918,
the
Ukrainians
of
Eastern
Ga-
licia
(also predominantly
Ukrainian
and
anciently,
According
to the International
Encyclopedia,
the
entire Austrian
province
of
Galicia
(western
and
east-
ern) ... Ukrainians.
According
to
the
Encyclopedia
Brittanica,
the
former
predominate
in the West and
in
the
big
towns,
and
the
latter
in the
East.
According
to
official statistics
of the
Austrian
pro-
vincial
government
of
Galicia, prepared
and
published
by
leaders
of
Polish
political...
- 28
- 400
- 0
Báo cáo sinh học: " Viroporin potential of the lentivirus lytic peptide (LLP) domains of the HIV-1 gp41 protein" docx
Ngày tải lên :
18/06/2014, 18:20
... LLP
domains may be flip-flopping between a transmembrane
state and parallel association with the inner leaflet of the
lipid bilayer. On the other hand, the LLP domains may
possess different activities ... membranes. Additionally, it is the first example of a
direct comparison of structure and function of an entire
LLP-1 domain from the laboratory adapted HXB2 strain
of HIV-1 (i.e., LLP-1) with a natural ... characterization of two active
forms, and partial cDNA sequence of a precursor. Proc Natl
Acad Sci USA 1987, 84:5449-5453.
3. Zasloff M, Martin B, Chen HC: Antimicrobial activity of syn-
thetic...
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- 0
