determination of the extracellular enzyme activity in soil

Báo cáo Y học: Brassica napus soluble epoxide hydrolase (BNSEH1) Cloning and characterization of the recombinant enzyme expressed in Pichia pastoris docx

Ngày tải lên : 31/03/2014, 08:20

... Lambda Zap II using R408 helper phage coinfection but was found to lack the initial bases of the 5¢-end (including the initiating methionine codon) Ó FEBS 2002 5¢-Rapid ampliﬁcation of cDNA ends ... BNSEH1 enzyme Results from two experiments are shown Effects of inhibitors on recombinant BNSEH1 enzyme activity Fig Kinetic analysis of recombinant BNSEH1 Enzyme activity was determined using TSO ... contained two ambiguities Five of the 18 clones sequenced differed from the cDNA by having a T instead of C at positions 145 and 249, respectively Neither of the two variants resulted in amino...

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Determination of the active ingredient produced in the artificial cultivated cordyceps sobolifera

Ngày tải lên : 08/01/2017, 20:58

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Báo cáo khoa học: Identiﬁcation of preferred substrate sequences for transglutaminase 1 – development of a novel peptide that can efﬁciently detect cross-linking enzyme activity in the skin pot

Ngày tải lên : 23/03/2014, 06:20

... Analysis of the reactivity of the K5 peptide with casein in the presence of TGases The time-dependent incorporation of biotinylated peptides (pepK5, pepK5QN and pepT26) into casein was examined in the ... effect on the reactivity of the peptide Assessment of the K5 sequence for reactivity and speciﬁcity in the peptide form To assess the reactivity and speciﬁcity of K5 in the peptide form, biotinylated ... pepK5QN was not incorporated into casein, also acting as glutamine-donor substrate, in the presence of either TGase or TGase This result indicates that the lysine residue in the sequence of K5 does...

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Báo cáo khoa học: The C-terminal region of the proprotein convertase 1⁄ 3 (PC1⁄ 3) exerts a bimodal regulation of the enzyme activity in vitro pdf

Ngày tải lên : 30/03/2014, 08:20

... cells expressing the recombinant mPC1 ⁄ 3, the presence of the 87 kDa form in excess of the 66 ⁄ 71 kDa facilitates isolation of the enzyme and helps in maintaining the enzymatic activity at a ... thus insuring cell-surface anchoring [37] In other cases, the CT-peptide contains integral transmembrane motifs affecting the sorting and recycling of furin and PC5 ⁄ 6B [38–40] In the case of ... cooperate in the activation of an enzyme In the case of proPC1 ⁄ 3, removal of the various structural and functional domains is a sequential and coordinated event culminating in removal of the CT-peptide...

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Tài liệu Báo cáo " Determination of the annual beta dose by meaming beta activity using the liquid scintillation technique " ppt

Ngày tải lên : 13/02/2014, 04:20

... Presently, in the ancient pottery TL dating the following steps for the determination of annual dose rate are: (i) place the thermoluminiscnece dosimeter at the field; (ii) determine the environmental ... determine the correct PDD (Pulse Decay Discriminator) setting The optimum setting is the setting where there is equal and minimum spill of alpha pulses into the beta MCA and beta pulses into the ... to determinate the annual gamma dose in thermoluminesccence dating In the consistency with the experimental results it was shown that the more accurate the beta dose evaluation is, the more reliable...

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Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf

Ngày tải lên : 20/02/2014, 02:21

... productive binding of steroid substrates: in the ﬁrst, a (small) sub population of enzyme binds directly the steroid in the reactive conformation, with the remainder of the enzyme binding the unreactive ... of the enzyme- steroid complex We conjectured that the reduced form of the enzyme might direct binding of the steroid in the ﬂipped conformation, thus facilitating catalysis Our structure of the ... perturb the overall framework of the enzyme or the active site The active sites of the enzymes are shown in stick format with the sigmaA weighted 2F0-Fc density for the Y186F mutant displayed in blue...

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Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx

Ngày tải lên : 22/02/2014, 07:20

... position 366 while all other proteins of the CYP11B family including CYP11B2 of the guinea pig have a basic residue (arginine or lysine, respectively) at the corresponding position These two basic residues ... 6) for the remaining introns Finally, the 3¢ UTRs of the guinea pig CYP11B genes show only 42% similarity whereas the 3¢UTR of the guinea pig CYP11B2 gene shares up to 72% identity with the corresponding ... function of the hormonal system We ﬁrst cloned the genes of the CYP11B family of the guinea pig The 11bhydroxylase of the guinea pig showed higher substrate speciﬁcity than the aldosterone synthase In...

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Comparison of the obstetric anesthesia activity index with total delivery numbers as a single denominator of workload demand in Israeli maternity units doc

Ngày tải lên : 05/03/2014, 15:20

... to develop a single denominator of obstetric anesthesia activity to offset this heterogeneity The obstetric anesthesia activity index (OAAI) The majority of anesthesia workload in the labor ward ... [8] The current study is the first stage in an approach that will attempt to define the adequacy of obstetric anesthesia workforce in relation to activity The epidural component of the OAAI includes ... single workload denominator Competing interests There are no competing interests declared for any author Author information Yehuda Ginosar is Senior Lecturer of Anesthesia and Director of the...

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Báo cáo Y học: Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types pptx

Ngày tải lên : 08/03/2014, 22:20

... quantification of the increase in incorporation of radioactivity detected in the GST–ATF2 fusion protein with respect to the HA epitope The results are the mean of four independent experiments with the ... those of CD53, CD9 or CD37 [15] However, despite the knowledge of some of the effects induced by tetraspanin proteins and the proteins with which they interact, the identification of the signalling ... detected in the anti-HA immunoprecipitate in an individual experiment At the bottom is the quantification of the level of the activation of JNK activity induced by CD53 ligation in three independent...

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Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf

Ngày tải lên : 17/03/2014, 11:20

... characterize the recombinant laccase from A niger Immunodetection of the recombinant laccase and expression of the corresponding gene in A niger Production of the recombinant laccase for the construction ... 80 60 40 20 pH Fig Eﬀect of the pH on the activity of the puriﬁed laccase pH in the range of 2.5–8 were tested with 500 lM ABTS (d) and 17 lM of syringaldazine (j) as the substrate Ó FEBS 2002 ... active protein but with an hyperglycosylation increasing the molecular mass to 110 kDa as compared to the 70-kDa wild-type protein [14] The production level of the recombinant protein in Pichia...

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Báo cáo Y học: A functional role of the membrane-proximal extracellular domains of the signal transducer gp130 in heterodimerization with the leukemia inhibitory factor receptor pot

Ngày tải lên : 18/03/2014, 01:20

... epitopes of gp130 are involved in ligand binding: the Ig-like domain and the CBM [9–11] In the case of LIF-induced heterodimerization of LIFR with gp130, the cytokine ﬁrst binds to the Ig-like domain ... motif of the interferon-c receptor resulting in a chimeric protein designated gp130c In order to investigate the role of the membrane-proximal domains of gp130 in receptor activation, the FNIII-domain ... gp130 in the membrane-proximal six domains This is likely to result in the same distance between the cell surface and the ligand-binding epitopes of both receptors Deletion of a single domain in the...

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Đề tài " Determination of the algebraic relations among special Γ-values in positive characteristic " pdf

Ngày tải lên : 22/03/2014, 16:20

... bj ¯ ¯ The ring k[t, σ] contains both the noncommutative ring k[σ] and the commu¯ as subrings The ring Fq [t] is contained in the center of the ring tative ring k[t] ¯ ¯ k[t, σ] The ring k[t, ... ((1/T )) = the | · |∞ -completion of k an algebraic closure of k∞ the | · |∞ -completion of k∞ the algebraic closure of k in C∞ ¯ a ﬁxed choice in k of a (q − 1)st root of −T the subring of the power ... study of the proof of Yu’s theorem Roughly speaking, the points of view adopted in the two theorems correspond as follows If H = Hom(Ga , E) is the dual t-motive deﬁned over ¯ k corresponding canonically...

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Báo cáo khoa học: Suppression of nuclear factor-jB activity in macrophages by chylomicron remnants: modulation by the fatty acid composition of the particles pot

Ngày tải lên : 23/03/2014, 04:20

... from the gut to the liver in CMR in the postprandial phase In this study, we investigated the effects of CMR on NF-jB activation in macrophages and determined whether these are modulated by the ... is in uenced by the fatty acid composition of the particles Phosphorylation of p65–NF-jB plays a critical role in regulating its transcriptional activity To further investigate the effects of the ... degradation of IjBa, thus allowing translocation of NF-jB to the nucleus [20] To determine whether modulation of IjBa serine phosphorylation status plays a part in mediating the inhibitory effects of...

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Báo cáo khoa học: Activity of the plant peptide aglycin in mammalian systems potx

Ngày tải lên : 23/03/2014, 09:21

... indicate beginning of washing An aglycin interacting protein is present in the pancreatic extract 0.02 Da of that for the fully oxidized peptide, showing disulﬁde bridges to be intact in the biologically ... [12,14,15] Due to the similarity with animal insulin in binding the basic 7S globulin and stimulating its protein kinase activity [16], the kDa soybean peptide was initially designated leginsulin [12] ... emphasized that even though aglycin, like insulin, in uences the concentration of glucose in blood, the effect is opposite that of insulin, increasing rather than decreasing the blood glucose concentration...

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Báo cáo khoa học: Determination of the reopening temperature of a DNA hairpin structure in vitro pptx

Ngày tải lên : 23/03/2014, 13:20

... from carrying out primer extension at 22 °C Fast annealing may decrease the likelihood of the self-folding of the template DNA, while it may increase the probability of primer binding to the template ... bases at the 3¢-end of primer In this work, the reopening of the hairpin structure and the subsequent primer extensions were attempted by dividing the reaction into aliquots, and incubating at ... was used as the sequencing primer Determination of the hairpin reopening temperature Oligonucleotide primer was mixed with single-stranded template DNA at a ratio of : in the annealing buffer...

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Báo cáo khoa học: Determination of the metal ion dependence and substrate speciﬁcity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl pot

Ngày tải lên : 30/03/2014, 15:20

... during chromatographic purification of BphH The apoenzyme can, however, be reconstituted with Mg2+ in vitro and the specific activity of the purified enzyme in the presence of saturating amounts of ... method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Laemmli UK (1970) Cleavage of structural proteins during the assembly ... rather than just a substrate binding role Possible roles of the cation include the activation of water for the hydration reaction and ⁄ or the stabilization of the anion transition state In the...

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Báo cáo khoa học: Comparative importance in vivo of conserved glutamate residues in the EX7E motif retaining glycosyltransferase Gpi3p, the UDP-GlcNAc-binding subunit of the ﬁrst enzyme in glycosylphosphatidylinositol assembly doc

Ngày tải lên : 30/03/2014, 20:20

... vivo The lowering of in vitro GlcNAc-PI synthetic activity upon introduction of the C301A mutation into Gpi3p may mimic the observed in vitro inactivation of GlcNAc-PI synthesis by alkylating ... possible that the sensitivity of in vitro activity to alkylating agents is due instead to modiﬁcation of other Cys residues in the GlcNAc-PI synthetic complex In summary, the results of our mutagenesis ... Ala without abolition of the catalytic activity of the representative enzyme studied [22] Deﬁnition of the roles of the EX7E Glu residues, and of signature amino acids in other glycosyltransferases,...

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Báo cáo lâm nghiệp: "Influence of the form of nitrogen nutrition reductase activity in young black locus" doc

Ngày tải lên : 09/08/2014, 04:20

... activity decreased in the previous leaf and the highest enzyme activity was found again in the new leaf (Fig 2) When the nitrate supply was withdrawn, the enzyme activity recovered its minimum value ... of nitrate of leaf NR activity of nodulafed plants Administration of mM NaN0 to 11 mo old nodulated plants did not increase leaf NR activity, whereas the 10 mM NaN0 dose induced high enzyme activity ... NR activity (6-9 nmol N02-mg- measured in vivo in the leaves of young nitrogen-deficient R pseudoacacia; its role is unknown, but its decrease, concomitant with the advent of the N activity, indicates...

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Báo cáo y học: " Angiotensin-converting enzyme 2 autoantibodies: further evidence for a role of the renin– angiotensin system in inflammation" potx

Ngày tải lên : 12/08/2014, 14:22

... expression potentially contributing to the protective mechanisms of these therapies There is increasing evidence for the interplay of the renin–angiotensin system and in ammatory events [5] Pre-eclampsia ... study is the first to identify autoantibodies that attenuate enzyme activity in a patient population The current findings are of potential importance in our understanding of the role of circulating ... against ACE2 may undermine this compensatory response Indeed, identification of endogenous ACE2 inhibitors is important in lieu of optimizing the therapeutic benefits following administration of...

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Báo cáo khoa học: "SOFA is superior to MOD score for the determination of non-neurologic organ dysfunction in patients with severe traumatic brain injury: a cohort study" doc

Ngày tải lên : 13/08/2014, 02:24

... hypotension MAP 5a or epinephrine ≤0.1a or norepinephrine ≤0.1a Dopamine >15a or epinephrine >0.1a or norepinephrine >0.1a Hematologic: platelet ... presentation to the trauma centre of or less in the absence of sedation; need for intracranial pressure monitoring; or the presence of a clinical herniation syndrome as verified by the attending physician ... (Microsoft Access; Microsoft Corporation) Missing values were replaced between a preceding and subsequent value with the lower of the two scores In the absence of a preceding or subsequent value, the...

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