... roll off, and spectral flux Subband features were octave-based (7 subbands from to kHz) and consist of the minimum, maximum, and average amplitude value for each subband The root mean square of an ... strength ofpeaks then was used as an estimate for the strength of the rhythm Auto-correlation delivered information about the regularity of the rhythm and the common divisor of the correlation peaks ... precision (pre), and mean recall (rec) for training with the training and development instances of AllInst, and testing on those of AllInst, Min2/4, and Min3/4 NoLyr feature set, V3 and A3 tasks,...
... processing and modeling structure of protein.8–10 Rezaie et al.7 has studied the simultaneous determinationof cobalt(II) and nickel(II) based on formation of their complexes with pyrolidine and carbon ... layer and 60 neurons in the hidden layer was successfully applied in simultaneous determinationof Co(II) and Ni(II) The aim in this study was the simultaneous determinationof Pb(II) and Cd(II) ... Stock standard solution (1000 mg/l) of Pb(II) and Cd(II) was prepared by dissolving 1.1261 g of Pb(NO3)2 (Fluka AG) and 1.0271 g of Cd(NO3)2 4H2O (Hamburg) in 1000 ml volumetric flasks and diluted...
... employed and the resolution of sildenafil and vardenafil decreased And the ionization of all analytes was greatly suppressed, sildenafil and tadalafil gave no signal even in the SIR chromatogram, and ... equipped with an ESI probe and quadrupole analyzer The control of system and data acquiring was performanced with Masslynx3.5 workstation (Waters) The standards of sildenafil and tadalafil were obtained ... flask, mixed and diluted to volume to yield a mixed standard solution Then, 5, 2, 1, 0.5, and 0.1 ml of the mixed standard solution were transferred to five 10-ml volumetric flasks, and diluted...
... space (a land area of 712.4 sq km) and limited natural resources, and heavily depends on imported energy and water For instance, it imported 99.84% of energy (The World Bank, 2009) and 40% of water ... planning and design of estates; construction of public flats and related facilities; sale or rental of public units along with the provision of mortgages; preservation of the resulting estates; and ... low scale of change refers to minor repair of a building; 2) substantial scale of change could mean major repair of a building; and 3) drastic scale of change refers to reconstruction of a new...
... thousands of hours, and tolerate thousands of start and stop cycles without failing Second, it must be able to run smoothly with minimum vibration, due to the tight tolerances of the platters and ... University of Singapore t (2.10) the slot pitch, and 23 Chapter2 Review on Computer Modeling and Analysis of PM Brushless DC Motors halfof the peak value of flux reduction in the center of the slot ... orginal design and the optimal design 169 Table 6.5 Determinationof cogging torque period (p = 3) 170 National University of Singapore IX List of Tables Table 6.6 Determinationof cogging...
... STRUCTURE DETERMINATIONOF KREMEN1, DICKKOPF1 AND MeCP2 VINDHYA B.N.REDDY A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE ... structure determinationof Krm1, Dkk1FL and the Krm1-Dkk1Cys2 complex We have been able to express and purify one of the crucial domains of Dkk1 from Mus musculus, proved to be necessary and sufficient ... support and knowledge he has imparted to me I warmly thank our collaborators from the University of Pennysylvania, Prof Sarah Miller and Prof Mariuz Wasik for helping us with the start of all...
... Extraction yield vs stirring speed of NSAIDs and clofibric acid Two-phase LPME extraction profile vs extraction time of NSAIDs and clofibric acid Chromatograms of NSAIDs and clofibric acid (at 10ppb) in ... commonly prescribed to relieve inflammation and pain, and they include ibuprofen, diclofenac, naproxen, ketoprofen, celecoxib and rofecoxib Ibuprofen and other similar pain-relieving drugs are ... evaluate the presence of acidic NSAIDs (ibuprofen, naproxen and ketoprofen), and another acidic API (clofibric acid) in aqueous matrices 3.2 Experimental 3.2.1 Chemicals and materials Trimethylphenylammonium...
... weakness of bands of fragments I and IV and the appearance of fragment II in the degradation of the Tyr146 !His/Asn147 !Ser mutant (panel d) indicated a significant decrease in the rate of cleavage of ... same half- lifeand kinetics in both the presence and the absence of Ca21 ions, indicating that the Tyr146 !His and the Asn147 !Ser substitutions altered neither the rate nor the kinetics of autolytic ... presence of a conserved autolytic site, Arg117, in the interdomain loop is essential to its autolytic inactivation [11] The control of half- life by the q FEBS 2001 Control of chymotrypsin half- life...
... libraries [4,29] The initial aim of this study was to investigate whether the modification of the half- lifeof an active intrabody could simultaneously affect the half- lifeof the target in the same ... eliminated by the proteasome machinery and that this can ultimately lead to cellular irregularity and cell death [30] On the other hand, the importance of the half- lifeof scFvs for activity in an intracellular ... widely used because of its intracellular stability (half- lifeof more than 20 h [34]) As the bound scFv molecules showed an extended halflife, this suggests that the stability of the enzyme was...
... STD and normal TOCSY spectrum of the PDTRP/SM3 complex are shown The peaksof Arg4 and Pro5 are so low in intensity that they not appear at the intensity cutoff shown Signals of Pro1, Asp2 and ... resonances of Pro1 and Asp2 have the highest intensities in the STD spectrum, signals of Thr3 are of medium intensity, while the signals of Arg4 and Pro5 have the lowest intensity The d-protons of Pro5 ... intraresidue contacts of Arg4) and between Thr3 and Pro5 the trNOE build-up of the b-protons of Asp2 was taken as reference For contacts between Arg4 and Pro5 the d-protons of Pro5 acted as reference...
... sheets, subjected to TLC and quantication of radiolabelled ADP and AMP The kinetics of [3H]AMP and [3H]ADP formation in the absence of tRNAGlu (black symbols) or of both L-Glu and tRNAGlu (grey symbols) ... GluRS and GluTR, whose spectrum and DLS signal are shown in Fig S11 The migration positions of GluTR and GluRS, were determined by comparison with those of a homogeneous sample of GluRS (S) andof ... sequence and the mass of the fragments, and from the kinetics of the process (Figs S7S9 and Table S5) we concluded that the main sites of proteolytic cleavage are at the C-terminus of the predicted...
... weight of softwoods, 1530% of hardwoods and up to 30% of annual graminaceous plants [7,8] Endo-1,4-b-xylanases (EC 3.2.1.8) are O-glycoside hydrolases that catalyze a random hydrolysis of internal ... the study of protein structure and function Of particular interest is the use of ESIMS in the studies of noncovalent interactions [5254], as valuable parameters such as stoichiometry and binding ... spectrum of 10 lM TRX II in 10 mM ammonium acetate (pH 6.8) at m z 29203060 showing the group ofpeaks representing monomers (charge state 7+) and noncovalent dimers (charge state 14+) of TRX II and...
... aroma of stagnant pools and beauty in the sparkling hues of the gutter, and revel in adding tints and pictures to the lifeand death of a weasel, lending enchantment to the lifeof a vagabond, and ... state of a respectable and civilised life, reside in houses which, in 99 cases out of 100, are in the lowest and most degraded part of the towns, among the scum and offscouring of all nations, and ... bags of gold and cotton and ladies and gentlemen desirous of listening to my humble tale of neglected humanity, and the outcasts of society, commonly called "Gipsies' children." Dr Gladstone, of...
... of the Physical andLife Sciences Research at the Intersection of the Physical andLife Sciences Committee on Research at the Intersection of the Physical andLife Sciences Board on Physics and ... sequence and learn how it relates to health and disease, aging, and the quality oflife Grand Challenge 4. Interactions of the Earth, Its Climate, and the Biosphere Many of the most challenging and ... Intersection of the Physical andLife Sciences 10 R e s e ar c h at the Intersection of the Physical andLife Sciences by a small number of fundamental laws They seek to understand how small bits of matter—atoms...
... apply to the determinationof the composition or concentration of fragrance components in products 1.4 The term “Executive Officer” as used in this document means the Executive Officer of the Air ... analysis of samples to determine the weight percent of total volatile material, using US EPA Reference Methods 24/24A, ASTM D 2369-01 3.3.2 Determinationof sample water content For determinationof ... analysis of samples to determine the weight percent of total volatile material, using US EPA Reference Methods 24/24A, ASTM D 2369-01 5.3.2 Determinationof sample water content For determination of...
... to the determinationof mobile metal fractions usually results in unrealistically large amounts of extractable Mn, Fe, Cu, and Zn, and underestimation of Ca, Mg, K, and probably Co, Ni, and V ... implications (e.g., during dredging operations) of land disposal of waste material, of acid precipitation, of redox changes in subsoil, andof ingestion of polluted urban dust, can be qualitatively ... hampered a good comparability of data, was the lack of suitable reference materials that precluded control of the quality of the measurements Thus, standardization of leaching and extraction schemes...
... Purification and molecular mass determinationof PPL2 (A) MALDI-TOF mass determinationof native PPL2 purified by affinity chromatography as illustrated in the insert Insert: the fraction of a Parkia ... evaporated to dryness under a stream of nitrogen and mild heating with a hair dryer To eliminate salts and proteins from the reaction mixture, 1.5 mL of chloroform and mL of distilled water were added ... dimensions of approximately 50 · 40 · 25 A, including three disulfide bonds (Cys20–Cys67, Cys50– Cys57 and Cys158–Cys187) and five cis peptide bonds Two of the cis peptide bonds of PPL2 (Gly147–Lys148 and...
... Case was the poorest of the package and Selznick lost money on it Spellbound and Notorious well made up for the loss, however bringing millions of dollars into the Selznick coffers But now Hitchcock ... himself of a murder charge because the actual murderer has revealed himself to Clift in the sanctity of the confessional The problems of Church and State play a major part in this detailed film and ... widescreen VistaVision and TechniColour added a new brilliant gloss and spectacle to the production filmed in England and on location in the Mideast Doris Day, whom Hitchcock had seen and liked, was...
... area of DDT degradation peaks (DDE DDD) x 100 TIC area of total DDT peaks (DDT DDE DDD) 525.2-18 % endrin breakdown= TIC area of endrin degradation peaks (EA EK) x 100 TIC area of total endrin peaks ... detection limits 9.3.5 Develop and maintain a system of control charts to plot the precision and accuracy of analyte and surrogate measurements as a function of time Charting of surrogate recoveries ... dechlorinated and acidified immediately prior to fortification with internal standards and surrogates, and extraction using the same quantities of acid and sodium sulfite described above 8.2.2 Atraton and...
... facilitated the determinationof both thermodynamic and kinetic properties of NR1, and as we have shown for CPR and P450 BM3 (23,24,28) the properties of the flavin binding domains of NR1 are likely ... reduction of substrate (DCPIP or ferricyanide) at appropriate wavelengths (600 nm and 420 nm, respectively) Results are the mean and standard deviation of triplicate assays For the determinationof ... spectroelectrochemical titrations of the isolated FAD/NADPH and FMN domains of NR1 enabled the determinationof the midpoint reduction potentials for the oxidized/semiquinone (E1) and semiquinone/hydroquinone...