detection and enrichment of hematopoietic stem cells by side population phenotype
Augmentation and differentiation of hematopoietic progenitor cells by CD137
... 1.1 Hematopoietic stem and progenitor cells (HSPC) Stem cells hold great promise for regenerative medicine and the study of cell and tissue differentiation Hematopoietic stem and progenitor cells ... of CD137 and CD137 ligand on small subsets of bone marrow cells Activation of hematopoietic progenitor cells – CD34+ cells in the human system and Lin-, CD117+ cells in the murine system – through ... TABLE OF CONTENT iv ABSTRACT x LIST OF TABLES xi LIST OF FIGURES xii LIST OF ABBREVIATIONS xvi CHAPTER INTRODUCTION 1.1 Hematopoietic stem and progenitor cells (HSPC) 1.2 Hematopoiesis and myeloid...
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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells ... methylation profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing ... establishment of embryonic stem (ES) cells, which maintain the ability to form all types of cells in the body, and can differentiate into a variety of cell types in vitro [8] The use of ES cells in...
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Báo cáo y học: "Controlling Osteogenesis and Adipogenesis of Mesenchymal Stromal Cells by Regulating A Circadian Clock Protein with Laser Irradiation" pptx
... distribution of mCRY1 and mPER2, and real-time PCR quantification of mCRY1 mRNA after laser irradiation Intracellular distribution of mCRY1 and mPER2 after blue laser irradiation were shown in Figs 1a and ... feedback loop of circadian rhythm regulation.7 The mammalian Period proteins (PER1 and PER2) and Cryptochrome proteins (CRY1 and CRY2) act as negative regulators of transcription driven by the BMAL1/CLOCK ... is the shutdown of CLOCK- and BMAL1-driven transcription by CRY proteins BMAL1 and CLOCK contain two basic helix-loop-helix domains and bind E-box elements (CACGTG) in the Per and Cry clock genes...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... from subsequent isolation and re-plating of the ICM cells, which are then screened for stemness by a variety of recognized markers Once in stable culture, ESCs are capable of either symmetric (clonogenic) ... further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole embryos or compromising ... Objective assessment of ESC colonies Although considerable resources are required to harvest and propagate ESCs, effective methods to verify stemness and monitor quality in such cells are also needed...
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A role for chondroitin sulfate proteoglycan in regulating the survival and growth of neural stem cells
... during injury, stem cells can adopt this form of cell division to expand and replenish the depleted stem cell pool (Morrison and Kimble, 2006) 1.4 1.4.1 Types of stem cells Embryonic stem cells During ... 1.3 Symmetrical and asymmetrical division 1.4 Types of stem cells 1.4.1 Embryonic stem cells 1.4.2 Somatic stem cells 1.4.3 Stem cells and cancer ... some tissues such as the skin and blood are constantly being replaced, and this requires a specialized population of cells, the stem cells Stem cells are uncommitted cells that have the potential...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... feasibility of significant mobilization of cells expressing hematopoietic stem cell and EPC markers and properties The area of nutritional modulation of the stem cell compartment offers significant ... represented clusters of more than 50 cells circumscribed by spindle shaped cells and were counted by microscope As the number of colonies depends on the number of plated cells, normalization of colony number ... mobilization of cells expressing hematopoietic stem cell markers Data is presented as percentage of mononuclear cells in Additional File Analysis of the number of the progenitor cells in circulation by...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the limbs, ... of primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant ... factor analysis of variance) and statistical significance was determined by student's t-test, and level of P < 0.05 was considered significant Results Transgene expression by aggregates of genetically...
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Báo cáo y học: " Enhanced self-renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene Sall4" doc
... HSC/HPCs by week 20 and week 50 (Figure 3b) Further more, these Ly5.2+ cells were able to reconstitute granulocyte, T cells, and B cells, as well as immature blood cells, as indicated by expression of ... mediated by GFP control cells was not detected When the number of infused cells was raised to × 105 cells, both Sall4A and Sall4B transduced cells repopulated of recipients, whereas GFP transduced cells ... that overexpression of Sall4 increased the repopulation activity of HSC/HPCs Of note, however, though HSC/HPCs were expanded by sustained ectopic expression of Sall4 isoforms and exhibited enhanced...
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IMPACT OF ALCAM (CD166) ON HOMING OF HEMATOPOIETIC STEM AND PROGENITOR CELLS Mariya Aleksandrova
... HOMING OF HEMATOPOIETIC STEM AND PROGENITOR CELLS The potential of hematopoietic stem cells (HSC) to home and to anchor within the bone marrow (BM) microenvironment controls the ability of transplanted ... hematopoietic cells to ALCAM expressing components of the hematopoietic niche via dimerization We investigated the role of ALCAM on the homing abilities of hematopoietic stem and progenitor cells ... Isothiocyanate HN Hematopoietic Niche HSC Hematopoietic Stem Cells HSPC Hematopoietic Stem and Progenitor Cells Marrow ECM Marrow Extracellular Matrix MSC Mesenchymal Stem Cells OB Osteoblasts...
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báo cáo hóa học:" Implantation of neural stem cells embedded in hyaluronic acid and collagen composite conduit promotes regeneration in a rabbit facial nerve injury model" doc
... were used to observe, record, and analyze animal behaviour in terms of signs and extents of muscular atrophy of lips, blink reflex, and ear motion of animals before and 12 weeks after peripheral ... immunohistochemical staining of BrdU and S-100 Representative images of immunohistochemical staining of BrdU and S-100 A: Localization of darkly brownish stained BrdU+ cells to transected tissues of rabbits having ... transection and implant of NSC and HA-collagen scaffold for 12 weeks (400× magnification) C: An array of fascicles of relatively uniform size in tissues of rabbit after facial nerve fiber transection and...
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báo cáo hóa học:" Activation of human B cells by the agonist CD40 antibody CP-870,893 and augmentation with simultaneous toll-like receptor 9 stimulation" doc
... both memory and naïve B cells can be activated by the drug CP-870,893, and this CP870,893 effect can be increased by the addition of CpG ODN 2006 Naïve B cells, as defined by lack of CD27 expression, ... control hIgG2 Cells were analyzed by flow cytometry for viability and expression of cell surface molecules at baseline and at 24 and 48 hr subsequent to stimulation A concentration of ug/ml of CP870,893 ... expression CD40-ligand (CD40L), also known as CD154, is the chief ligand for CD40 and is expressed primarily by activated T cells and platelets [10,11] The interaction of CD40 and CD40L represents...
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Báo cáo hóa học: "Future research and therapeutic applications of human stem cells: general, regulatory, and bioethical aspects" ppt
... functional repair of myocardial infarction, and in stroke [31,32] Other types of stem cells Hematopoietic stem cells together with mesenchymal stem cells, the so-called side population , and multipotent ... Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) from somatic cells are revolutionizing the field of stem cells Obtained by reprogramming somatic stem cells of a patient through ... progenitor cells (MAPCs), are the stem cells Page of 15 forming bone marrow [33] Their role is maintenance and turnover of blood cells and immune system The high rate of regeneration of the liver,...
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Báo cáo hóa học: "Hybrid approach of ventricular assist device and autologous bone marrow stem cells implantation in end-stage ischemic heart failure enhances myocardial reperfusion" doc
... Collection and assembly of data AK Data analysis and interpretation, collection and assembly of data CP Conception and design, data analysis and interpretation SW Conception and design, data analysis and ... efficacy of the method Since the net “healing” capacity of BMSCs is difficult to determine, imaging of transplanted stem cells is crucial in order to investigate the attitude of the engrafted stem cells ... a reduced number of functionally competent cells [17] Therefore, formation of myocytes and coronary vasculature cannot counteract the chronic loss of functional cells and Page of vascular structures...
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Báo cáo sinh học: " Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pot
... understood Hematopoietic stem cells (HSCs) contain specific markers on their surface such as CD34 and CD133 allowing for the isolation of relatively pure populations of these cells for in vitro study of ... because of the limited amount of sample from patients and normal donors On the other hand, in vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and ... Thailand and two normal donors All patients were children 3-12 years-old and had symptoms of severe anemia and hepatosplenomegaly Hemoglobin analysis showed no expression of the b-hemoglobin band...
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o cáo hóa học:" Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pptx
... understood Hematopoietic stem cells (HSCs) contain specific markers on their surface such as CD34 and CD133 allowing for the isolation of relatively pure populations of these cells for in vitro study of ... because of the limited amount of sample from patients and normal donors On the other hand, in vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and ... Thailand and two normal donors All patients were children 3-12 years-old and had symptoms of severe anemia and hepatosplenomegaly Hemoglobin analysis showed no expression of the b-hemoglobin band...
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Báo cáo hóa học: "Research Article Detection and Correction of Under-/Overexposed Optical Soundtracks by Coupling " pot
... 4.1 Distortion detection by center of gravity shifts The center of gravity of a spectrum (COG) is in a sense, the “mean” frequency, and this method is used for pitch detection and for audio restoration ... original image and its opening (or closing) in function of the size of structuring elements A divergence between the graph of openings and the one of closings means that the surface of the peaks ... image of the soundtrack using the method introduced by Brun et al [12] in order to binarize it and suppress the noise The application of a series of openings with structuring elements of increasing...
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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...
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Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot
... of antibodies was performed by immunofluorescence and immunohistochemistry Immunofluorescence detection Disclosure of secondary antibody binding was performed by incubation in 1:50 dilution of ... induce the differentiation of MSCs to a disc-like phenotype and direct the cells to form a new and appropriate matrix Materials and methods Mesenchymal stem cell source and extraction Bone marrow ... source are adult stem cells, in particular, bone-marrow-derived mesenchymal stem cells (MSCs) The use of these cells would allow an autologous approach, reducing the risk of rejection and infection...
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Báo cáo y học: "Hypoxic conditions increase hypoxia-inducible transcription factor 2α and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients" pps
... culture and cell surface epitope characterisation of IPFP cells The cells isolated from the IPFP proliferated in culture and reached confluence by day 14 The yield of cells by the end of passage ... CD105 (markers for mesenchymal stem cells) , and for CD29 (β1 integrin) The cells stained poorly for LNGFR and STRO-1 (markers on freshly isolated bone marrow stem cells) and sparsely for 3G5 (marker ... characterisation and flow cytometry of the IPFP cell population showed a similar staining pattern to that of bone marrow-derived stem cells, and although they stained poorly for STRO-1 and for LNGFR,...
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Báo cáo y học: " Detection and correction of false segmental duplications caused by genome mis-assembly" potx
... genome from the remainder of the experiments below By contrast, chimpanzee and chicken, assembled with PCAP, contain 16.7 and 14.4 Mb of sequence, spread across thousands of contigs, that appears ... covered by the span of the alignment of the contigs We found that, for the regions identified as mis-assembled in Table 1, 77.2% of the chicken contigs, 76.3% of the chimpanzee contigs, and 94.1% of ... cover >93% of the contig's length at >95% identity are saved as DCCs Alignments of size >300 bp and >95% identity that are consistent with the layout of DOCs and within 300 bp of the ends of both...
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