desorption ionization mass spectrometry for protein identification using peptide and fragment ion masses
Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... residue at position and leucine replacing methionine at position 23) formation as a function of guanidinium chloride concentration and pH The rate of (Pyr1)-ONC (M23L) formation was calculated ... glutamine residues of tryptic peptides of streptococcal PepM49 protein Resolution of cyclized peptides by HPLC and characterization by mass spectrometry Int J Pept Protein Res 34, 118–123 26 Arii, ... Characterization of the intramolecular cyclization reaction Pyroglutamyl formation From the set of data described in the preceding section, it was also possible to monitor the cyclization reaction as...
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Báo cáo khoa học: Novel strategy for protein production using a peptide tag derived from Bacillus thuringiensis Cry4Aa pptx
... (such as P20) for crystallization [2] The protein crystallization mechanism of the Cry toxin may prove beneficial for the expression of heterologous proteins in general Crystallization of the large ... special protein structure in the C-terminal extension; therefore, this extension can be utilized as a peptide tag to facilitate the crystallization of fused proteins The crystallization may protect ... isolated peptide tag may contribute to the establishment of novel and efficient expression systems for proteins Results Glutathione S-transferase (GST) fused with Cry4Aa peptides To determine the peptide...
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Tài liệu Báo cáo khoa học: "Learning with Unlabeled Data for Text Categorization Using Bootstrapping and Feature Projection Techniques" doc
... Friedman, and N Tishby, 2002, Unsupervised Document Classification using Sequential Information Maximization, In Proc of SIGIR’02, pp 129-136 Y Yang and J P Pedersen 1997, Feature selection in statistical ... text categorization (Slonim et al., 2002) Nigam studied an Expected Maximization (EM) technique for combining labeled and unlabeled data for text categorization in his dissertation He showed that ... Context-Clusters for At first, we automatically create keywords from a title word for each category using co-occurrence information Then centroid-contexts are extracted using the title word and keywords...
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Báo cáo y học: "A protocol for classifying normal- and flat-arched foot posture for research studies using clinical and radiographic measurements" pps
... derived from Scott and colleagues [7] The rationale for using SD standard deviations was to increase the likelihood of participants with flat-arched feet qualifying for inclusion via radiographic ... flat-arched foot as greater than two standard deviations from the normative mean (as reported by Scott and colleagues [7]) The rationale for using two standard deviations was to increase the likelihood ... review and editorial decision-making processes for papers they have co-authored Authors' contributions GSM, HBM and KBL conceived the idea and obtained funding for the study GSM, HBM and KBL...
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landslide hazard and risk assessment for road network using rs and gis a case study of xin man district, vietnam
... 1987 and 2000, which are use for the extraction of land surface temperature and landuse information 1:25000 scale standard topographic map has been digitized and the obtained contours were used for ... Reflection/Radiation of Electromagnetic Radiation Camera Scanner A/D conversion using a film scanner etc Primary Processing D/D conversion Digital Image Reconstruction / Correction Transformation ... critical to analysis For example, the location of a fire station or the locations where soil erosion is most severe are key considerations in using information In each case, what it is and where it is...
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Characterization of aptamer protein interactions using optical and acoustic biosensors
... Equation 2-7 12 Equation 2-8 12 Equation 2-9 13 Equation 2-10 13 Equation 2-11 13 Equation 2-12 13 Equation 2-13 14 Equation 2-14 14 Equation 2-15 14 Equation 2-16 14 Equation 2-17 15 Equation ... Equation 2-19 15 Equation 2-20 18 Equation 2-21 20 Equation 2-22 21 Equation 2-23 22 Equation 2-24 22 ix Equation 2-25 23 Equation 2-26 23 Equation 2-27 23 Equation 2-28 24 Equation 2-29 24 Equation ... n=5 for the QCM-D data on aptamers-thrombin-aptamer sandwich complex formation 83 viii List of Equations Equation 2-1 11 Equation 2-2 11 Equation 2-3 11 Equation 2-4 11 Equation 2-5 11 Equation...
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ICT for city management using information and communications technology to enable, engage and empower city stakeholders
... transportation, housing and land development, and environmental protection agencies The goal is to integrate publicspace-related information both for day-today management purposes and to inform policymakers’ ... departments—including land and housing development, environmental protection, and public safety agencies (for example in the reporting and handling of criminal cases and residents’ complaints) The Commission is ... Municipal Commission of Economy and Informatization According to the Commission, its priority is a “grid-style public management information system” that integrates information across various...
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Myocardial protection and therapeutic angiogenesis using peptide and embryonic stem cell transplantation
... dysfunction (Aukrust et al, 1999) Observation has been made that there is close relation between the expression level of IL-6 and gp130 and cardiac performance Ventricular expression of IL-6 and ... degradation and fibrosis formation 6 6 9 10 11 11 13 15 15 1.3 Overview of current surgical and pharmacological treatments for heart failure 16 1.4 Molecular and cellular approaches for heart ... hypertrophy, apoptosis and necrosis, ventricular dilatation, fibrosis formation and collagen degradation These changes over time lead to abnormalities in myocardial contractility and relaxation, to declined...
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Algorithms for peptide and PTM identification using tandem mass spectrometry
... concentration on algorithms for peptide and PTM identification using tandem mass spectrometry The main concern for algorithms in peptide identification is achieving fast and accurate peptide identification ... SOM and MPRQ for coarse filtering 79 Figure 17 Diagram for the peptide identification with PepSOM (a) SPC is used to score and rank candidate peptides (b) Candidate peptides are scored and ... and show the power of combination of tags, SOM and MPRQ techniques for peptide and PTM identifications The overall outline of my PhD dissertation is illustrated in Figure Peptide Identification...
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Tài liệu Real-time, on-line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry pdf
... reaction deviations In addition to real-time monitoring, tandem mass spectrometry (MSn) helps to identify unknown species, validate proposed intermediates and further understand the reaction mechanisms ... reactions using EESI-MS Figure Mass spectra of the Michael addition reaction recorded at 20, 60 and 300 min, respectively Inserts: molecular structures of PEA, PEAP and CPEAP 2995 (PAs) and remain ... chemical reactions using EESI-MS 2997 Figure Collision-induced dissociation spectra of some [MþH]þ ions of products and intermediates from the 4-DMAP-catalyzed acetylation reaction, including...
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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
... Calcium and peptide binding to folded and unfolded conformations of cardiac troponin C Electrospray ionization and Fourier transform ion cyclotron resonance mass spectrometry Eur J Mass Spectrom ... transform ion cyclotron resonance mass spectrometry: a primer Mass Spectrom Rev 17, 135 56 Smith RD (2000) Evolution of ESI -mass spectrometry and Fourier transform ion cyclotron resonance for ... CM (1989) Electrospray ionization for mass spectrometry of large biomolecules Science 246, 6471 52 Loo JA (2000) Electrospray ionization mass spectrometry: a technology for studying noncovalent...
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Báo cáo khoa học: Affinity purification-mass spectrometry Powerful tools for the characterization of protein complexes ppt
... limitations of peptide mass fingerprinting uses a combination of partial peptide sequence and mass information for protein identification (tandem MS or MS/MS) In this technique, a particular peptide ... called peptide mass fingerprinting (PMF) [28] In this technique, the masses of peptides from a tryptic protein digest are determined using matrix-assisted laser desorption/ ionization time-of-flight mass ... ÔlockedÕ within the peptide by the masses of the fragments that define the beginning and the end of the determined sequence Information on peptide sequence, peptide mass and fragment mass can be queried...
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báo cáo hóa học: " Method optimization and validation for the simultaneous determination of arachidonic acid metabolites in exhaled breath condensate by liquid chromatography-electrospray ionization tandem mass spectrometry" doc
... the preparation of the other standard concentrations for calibration and quality control material For the preparation of internal standards solutions commercially available [2H4] LTB4 and [2H4] ... solution for each labelled standard [2H4] LTB4 was used for the correction of leucotriene response values, whereas [2H4] PGE2 was used for prostaglandins and 8-isoprostane For quantification, ... analytical methods applied yet For a sensitive detection, including structural information, tandem mass spectrometry was used to determine unequivocally prostaglandins and leukotrienes This developed...
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facilitation of protein three-dimensional structure determination using enhanced peptide amide deuterium exchange mass spectrometry (dxms)
... protein conformational changes and protein- protein interactions Therefore, there is a great need for improvements in the speed and ease of determining and analyzing protein structures and protein ... denaturation and fragmentation; 3) mass spectrometry; and 4) peptide identification and mapping On-exchange of deuterium is performed under native conditions in D2O buffer The effects of ligand binding ... chromatography for direct tandem mass spectrometric analyses using an electrospray Micromass Q-TOF mass spectrometer and a Thermo Finnigan LCQ electrospray ion trap mass spectrometer For identification...
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Insights into protein kinase a activation using cAMP analogs and amide h 2h exchange mass spectrometry
... substitution at the equatorial oxygen for Rp-cAMPS and at the axial position for the Sp-cAMPS 2.Materials and Methods 2.1 Protein expression and purification 2.11 PKA RIα91-244 expression The ... Materials and Methods 2.1 Protein expression and purification 2.11 PKA RIα91-244 expression 2.12 PKA RIα91-244 purification 2.13 Equilibration of cAMP agarose resin 2.14 PKA-C expression ... particular and both CBDs in general, consist of three α-helices and eight β-strands (24) The helical regions make up the interface for PKA-C interactions while the β sheeted regions form the cAMP...
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Tài liệu Báo cáo khoa học: Si-face stereospecificity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic Archaea as determined by mass spectrometry ppt
... spontaneous reaction of H4MPT and H2CO and [14a-2H2]methylene-H4MPT by spontaneous reaction of H4MPT and 2H2CO [30] FprA from M marburgensis [20] and Mtd from Methanopyrus kandleri [26] were ... which catalysed the oxidation of F420H2 with O2 as the electron acceptor Samples of the assay were taken before and after the addition of Mtd and after the addition of FprA and analysed by MALDI-TOF-MS ... oxidation of F420H2 with O2 to F420 and H2O The 45 kDa protein contains FMN per mol and harbours a binuclear iron centre 5338 Results The following findings are important for the understanding...
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TECHNIQUES FOR THE ANALYSIS OF ORGANIC CHEMECALS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (ICP-MS) pptx
... calibrations in IPA Standard addition at 0, 5, 10 and 20 ppt, showing effective removal of 12C2 and 40Ar12C (potential interferences on 24Mg and 52Cr respectively) Conclusions For More Information ... sampled from a region of the plasma where the ionization is carefully controlled Thus, ionization of the elements of interest can be maintained, but the potential interfering polyatomic ions can be ... or consequential damages in connection with the furnishing, performance or use of this material Information, descriptions and specifications in this publication are subject to change without notice...
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Báo cáo khoa học: Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry pptx
... and #148261 were used for the measurement of the glucose (and trehalose), lactate, ammonia, and acetate concentrations, respectively Specifically for the determination of trehalose concentration, ... [45] The metabolite- and isotopomer balances are formulated based on a stoichiometric model for the intracellular metabolic reactions and describe the conservation of mass and isotopic label in ... isotopomer fractions of the corresponding metabolite pools, (iii) the equations relating the net and exchange fluxes to the forward and reverse fluxes of the network reactions, and (iv) the equations describing...
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Báo cáo khoa học: Construction of a novel detection system for protein–protein interactions using yeast G-protein signaling pdf
... was to establish a rapid and reliable method for the detection of protein protein interactions using yeast G -protein signaling In our system, protein protein interactions were detected utilizing ... based on G -protein signaling for detection of protein protein interactions, using a mutated Gc that lacks membrane-localization ability Our assay can be performed under conditions suitable for maximal ... transcription, growth arrest in the G1 phase, and polarized morphogenesis for mating One significant advantage of using G -protein signaling for the detection of protein protein interactions is that...
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Ionization Methods in Organic Mass Spectrometry pot
... either desorption or desolvation methods if they are to be analyzed by mass spectrometry Although ionization and desorption/ desolvation are usually separate processes, the term "ionization method" ... ionization and desorption (or desolvation) methods The choice of ionization method depends on the nature of the sample and the type of information required from the analysis So-called 'soft ionization' ... ionization' methods such as field desorption and electrospray ionization tend to produce mass spectra with little or no fragment- ion content Gas-Phase Ionization These methods rely upon ionizing...
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