desensitisation initiated by phosphorylation of the receptor can be subsequently follow
Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
Ngày tải lên :
22/02/2014, 04:20
... protein bearing beads, as shown below From these results, we concluded that the percentage of beads with bound chromatinÕ obtained with GST-fusion protein-bearing beads can be used as an index of the ... region of chromatin On the other hand, with the pretreatment of the chromatin with a low concentration of trypsin, the binding was suppressed strongly in the case of the NM fragment but not the ... Stimulation of the binding of LBR to chromatin by phosphorylation by a kinase(s) in a synthetic phase egg cytosol Chromatin binding of beads bearing GST–NK was increased by pretreatment with a synthetic...
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Tài liệu Báo cáo khoa học: The localization of FGFR3 mutations causing thanatophoric dysplasia type I differentially affects phosphorylation, processing and ubiquitylation of the receptor pptx
Ngày tải lên :
19/02/2014, 00:20
... other mutants (Fig 1C) The 130 kDa isoform of the K650M mutant was only weakly and variably detected in immunoblots Scanning densitometry of the gel further indicated that the intensity of the ... kDa) in the absence of ligand, whereas FGF was required to induce phosphorylation of the wild-type receptor (Fig 2A) By contrast, the 105 kDa nonglycosylated isoform of the K650M Variable phosphorylation ... within the Golgi system was prevented by blocking transport from the ER to the Golgi BFA had no effect on the relative lack of the 130 kDa isoform of the K650M mutant Endo H digestion of the immunoprecipitated...
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Báo cáo khoa học: Phosphorylation of the Saccharomyces cerevisiae Grx4p glutaredoxin by the Bud32p kinase unveils a novel signaling pathway involving Sch9p, a yeast member of the Akt / PKB subfamily pot
Ngày tải lên :
07/03/2014, 04:20
... modulated by nutrients [21] To verify whether the onset of conditions that modify the amount of Sch9p has an effect on the phosphorylation of Bud32p at Ser258, we compared the level of Bud32p phosphorylation ... recognizes the same target sequence as human Akt ⁄ PKB, further supporting the assumption of the similarity between the two protein kinases This raises the question of whether phosphorylation of Bud32p ... elongation We have then examined the effects of Ser258 phosphorylation of Bud32p on the transcriptional activity of the EKC ⁄ KEOPS complex by analyzing the activation rate of the galactoseinducible...
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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
Ngày tải lên :
16/03/2014, 12:20
... Based on these results, we discuss the role of phosphorylation- dephosphorylation events in the biological function of CNBP Fig (A) In silico analysis of zebrafish CNBP amino acid sequence The amino ... 20% with respect to the radioactivity incorporated in the wild-type protein (Fig 8B) CNBPS158A phosphorylation by PKA might be due to the high amount of PKA-Ca used in the phosphorylation assay ... consequently, may not be relevant Alternatively, CNBPS158A phosphorylation might occur by conformational changes in the molecule due to the mutation that affect the accessibility of other PKA phosphorylation...
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Báo cáo khóa học: Fidelity of targeting to chloroplasts is not affected by removal of the phosphorylation site from the transit peptide doc
Ngày tải lên :
23/03/2014, 12:20
... channel and the red chlorophyll fluorescence channel They are multiprojections of six or eight scans of the depth of the guard cell(s) expressing the GFP-fusion constructs (A) The targeting of pPSSU.tp.wt-GFP ... identified as such by the presence of stromules [20], indicated by the arrows in the higher magnification images The scale bar is 50 lm in the images on the left, and 10 lm in the higher magnification ... Alteration of the phosphorylation site did not impede targeting of the passenger GFP to the chloroplasts, nor was there any mistargeting to mitochondria The pattern of GFP fluorescence after targeting by...
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Báo cáo y học: "Development of mental health first aid guidelines on how a member of the public can support a person affected by a traumatic event: a Delphi study" doc
Ngày tải lên :
11/08/2014, 16:22
... centres) The first aider should be aware that the person may not remember all the details of the event The first aider should be aware that the person may suddenly or unexpectedly remember details of ... actions they can take and most of the actions recommended in these guidelines are not appropriate for the public While a number of guidelines have been written in the past several years for use by ... in the list of items to be assessed by the panel members (for further details see "Questionnaire development" below) The search term was 'trauma*' and all records for the 20 years leading to the...
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Báo cáo y học: ":The expression of the receptor for advanced glycation end-products (RAGE) in RA-FLS is induced by IL-17 via Act-1" ppsx
Ngày tải lên :
12/08/2014, 17:22
... mg/dL respectively The diagnosis of RA was confirmed by the revised criteria of the American College of Rheumatology [18] Informed consent was provided according to the Declaration of Helsinki and ... observations set the number of positive cells at the site We used the immunohistological criteria for classification of synovial tissues into “mild” and “severe” We evaluated the severity by the method ... Research & Therapy 2011, 13:R113 http://arthritis-research.com/content/13/4/R113 member of the superfamily of immunoglobulin type cell surface receptors [4] This receptor is strongly activated by cross-linked...
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Báo cáo y học: "Clustering of phosphorylation site recognition motifs can be exploited to predict the targets of cyclin-dependent kinase" potx
Ngày tải lên :
14/08/2014, 17:22
... cluster, and between the end of the cluster and the end of the protein We then took the minimum of these divided by the length of the protein to be the position We computed the foldedness as If ... identified the optimal cluster using SBN as described above To compute the position of the cluster, we calculated the distance between the start of the protein and the start of the cluster, and between ... compared the frequency of matches to the cy motif in the clb5 specific proteins with the frequency in the rest of the proteins above the cutoff (Figure 8b) Although the frequency of cy motifs in the...
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Tài liệu Venereal Diseases in New Zealand (1922) by Committee Of The Board Of Health pdf
Ngày tải lên :
17/02/2014, 14:20
... among the members of the Board to conduct an inquiry into the question of venereal diseases in New Zealand The following members are being asked to become members of the Committee, and the Chairman ... to the Chairman of the Board of Health, under date of the 20th June, 1922, from the Council of the New Zealand Branch of the British Medical Association The Board of Health duly considered the ... infected, but upon the health and well-being of the community as a whole This ignorance of the nature of the problem and of the grave issues involved naturally stands in the way of the evil being grappled...
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Tài liệu Báo cáo khoa học: Redox regulation of dimerization of the receptor proteintyrosine phosphatases RPTPa, LAR, RPTPl and CD45 pdf
Ngày tải lên :
18/02/2014, 17:20
... The ectodomains of both monomers in the dimer are required for this effect, suggesting that the epitope tag of one monomer is masked by the ectodomain of the other monomer in the dimer, and vice ... signals increased tyrosine phosphorylation of beta betacatenin through inactivation of the intrinsic catalytic activity of the receptor- type protein tyrosine phosphatase beta ⁄ zeta Proc Natl Acad ... Dimerization of many RPTPs may be driven by their transmembrane domain, since the transmembrane domains of 18 out of 19 RPTPs mediated dimerization of fusion proteins above background levels [41] The...
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Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx
Ngày tải lên :
19/02/2014, 18:20
... increase in phosphorylation of both isoforms was reduced by the coexpression of hPDE4D2 with 5-HT7 receptors (Fig 3C) This was also the case for ERK1 ⁄ phosphorylation (Fig 3D) Phosphorylation of Ser916 ... Gi-coupled receptors, induces the activation and phosphorylation of Ras-GRF1 Furthermore, Ras-GRF1 is also heavily phosphorylated upon agonist activation of GPCRs, but the exact role of these phosphorylations ... 60 kDa isoforms of Ras-GRF1 was increased following stimulation with serotonin The 60 kDa isoform of Ras-GRF1 seems to be expressed at a higher level than the 140 kDa isoform The serotonin-induced...
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Báo cáo khoa học: Differential recognition of heat shock elements by members of the heat shock transcription factor family ppt
Ngày tải lên :
07/03/2014, 00:20
... promoters These results show that the configuration of nGAAn units in the promoter is important in determining which hHSF members are involved in the regulation of the gene Footprint analysis of the ... recognition by human HSFs resulted in robust activation by hHSF4–VP16, without changing the magnitude of the heat shock response (cp3) The mutational analysis of the CRYGA and CRYGC HSEs suggests that the ... 5B) The observation that hHSF4–VP16 did not bind stably to sequences containing either 3P-like, gap-like or step-like HSEs might be explained by the divergence of GAA-like sequences from the canonical...
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Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf
Ngày tải lên :
07/03/2014, 03:20
... interpretation of the data suggests that the indicated basic region of ic3 of the D2 receptor and acidic region of the C-tail of the D1 receptor might be involved in the interactions between the two ... depended on the number of Arg residues that were replaced by Ala in the Arg-rich region of ic3 (residues 217–222) of the dopamine D2 receptor The efficiency of energy transfer in the wild-type of the ... residues in the C-terminal end of the D1 receptor, as well as the Arg-rich region of ic3 of the D2 receptor, not seem to take part in receptor homodimerization, but they influence D1–D2 receptor...
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Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot
Ngày tải lên :
07/03/2014, 17:20
... footprints by HbpR [19] and are used as such also in other figures Sequence numbers refer to the locations of the transcriptional start site of hbpC (B) Alignments of the sequences of three HbpR-binding ... impaired in binding of the R13 region Both of these patterns contributed to the loss of binding contacts of mutant 72 Construction of a DA HbpR derivative In order to study whether the A-domain was ... assembled as described in the main text Relevant restriction sites and the direction of transcription for the hbpR and the luxAB genes are indicated (A) (B) Detail of the region of the calmodulin-binding...
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Báo cáo khoa học: "GRAMMATICAL AN ALYSIS BY COMPUTER OF THE LANCASTER OSLO/BERGEN (LOB) CORPUS OF BRITISH ENGLISH TEXTS." potx
Ngày tải lên :
08/03/2014, 18:20
... was the frequency of potential tag A followed by potential tag B divided by the frequency of A Subsequently, it was changed to the frequency of A followed by B divided by the product of the frequency ... form The probability of tags being selected from the alternative lists is weighted according to whether the form occurs at the beginning of the sentence or elsewhere The future It should be made ... Revision of the programs and data files Revision of the word tag set has necessitated extensive revision of the word- and suffixlists The transition matrix will be adapted so that the corpus can be...
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Báo cáo Y học: Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types pptx
Ngày tải lên :
08/03/2014, 22:20
... At the bottom is the quantification of the level of the activation of JNK activity induced by CD53 ligation in three independent experiments The relative values are calculated by the ratio of the ... Alternatively the cells were left in the presence of the antibody for the complete length of this period In both cases the result was the same As shown in Fig 7, the ligation of the CD53 expressing ... In (A) the relative increase was determined with respect to the nonstimulated cells (point 0¢) In (B) the quantification was determined by the ratio of the signal of the radioactivity in the GST–Jun...
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Báo cáo Y học: Irregular spiking in free calcium concentration in single, human platelets Regulation by modulation of the inositol trisphosphate receptors ppt
Ngày tải lên :
17/03/2014, 17:20
... seems to be independent of the InsP3 receptor- mediated Ca2+ release It can thus be envisioned that, in intact platelets, the Ca2+ release evoked by U73122 stimulates the process of InsP3 receptor- mediated ... at higher Ca2+ levels the InsP3 receptors become desensitized Other factors determining the open probability of the receptor channels are the luminal Ca2+ concentration in the endoplasmic reticulum ... likely that the rate of Ca2+ pumping rather than the diffusion of released Ca2+ into the cytosol (as in bigger cells) determines the duration of the platelet spikes In many cell types, the global...
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Báo cáo khoa học: Phosphorylation of the arginine/serine dipeptide-rich motif of the severe acute respiratory syndrome coronavirus nucleocapsid protein modulates its multimerization, translation inhibitory activity and cellular localization pptx
Ngày tải lên :
23/03/2014, 07:20
... modulated by phosphorylation Effect of RS motif phosphorylation on oligomerization of the N protein To better understand the effect of RS motif phosphorylation on the biological function of the N protein, ... domain dimerization [26] Nevertheless, whether phosphorylation of the RS motif can modulate the functions of N proteins remains to be examined in detail Phosphorylation of SARS CoV-N protein RS motif ... that of the full-length N protein (data not shown) Taken together, these data suggest that the RS-rich region of the SARSCoV N protein is possibly phosphorylated by SRPK1 Phosphorylation of multiple...
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Báo cáo khoa học: Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex docx
Ngày tải lên :
23/03/2014, 11:20
... and the findings described below, suggest that the decrease of HAT in the plasma membrane should be interpreted as a lower level of the H+-ATPase–tubulin complex We therefore presume that the ... when used at 10 mm The blocking of the ATPase activating effect of glucose was not the result of a toxic effect of 2-deoxy-d-glucose, as subsequent incubation in the presence of 10 mm glucose resulted ... during the in vitro procedure, the amount of HAT in membranes from glucose-treated cells would be the same as that from glucose-untreated cells The possibility that the presence of glucose during the...
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