... items, therefore, may cover the content of the course book Or in other words, the test has content validity Besides the mean, the mode, the median and the range, we need to calculate the standard ... specification to see whether the items are actually testing what they are supposed to be tested, and whether the items are testing what the designers say they are Therefore, test’s content validity ... between the most and the least able testees R = 9-3=6 So, the range of the test is This is meant that the test involves the items that rank from the easiest to the most difficult ones and the test...
... location of the two sampling sites on the North Fork of the Shenandoah River, Virginia Investigation of Organic Chemicals Potentially Responsiblefor Mortality and Intersex in Fish of the North Fork ... Laboratory Friends of the North Fork of the Shenandoah River Prepared in cooperation with the Friends of the North Fork of the Shenandoah River Open-File Report 2008–1093 U.S Department of the Interior ... used forthe analysis of PAHs; the other was used for OC pesticide and total PCB measurements One of the SPMDs not containing PRCs in each canister was screened for estrogenic chemicals by the...
... although the binding data cannot unequivocally inform on the orientation of the sites, only their affinity for interaction with drugs The data presented here suggest that the ABCG2R482G isoform undergoes ... pumps is assumed to correspond to the outward-facing conformation The energy produced by the hydrolysis of ATP is harnessed forthe resetting of the transporter to the initial high-affinity, inward-facing ... with the membranes corresponded to nonspecific binding at sites other than the ABCG2R482G protein This fraction corresponded to 37 ± pmolÆmg)1, and therefore the specific binding component in the...
... targets forthe chemotherapeutic treatment of cancer and viral diseases because they catalyse the addition of the first phosphate group to the nucleoside analogue This primes the nucleoside for further ... and R105 It is evident from the kinetic results that mutation of E52 changes only the catalytic rate Forthe E52D mutant the Km value was approximately the same as forthe wild-type with dThd, whereas ... not show any activity must therefore be a consequence of the reactivity of the functional group and further support the hypothesis that E52 acts as an initiating base in the reaction R105 mutations...
... succinate/glucose-grown cells; therefore, the synthesis of the enzyme was inducible Table shows a summary of a typical enzyme purification The enzyme was purified 103-fold with an overall yield of 2% The specific ... accumulated via these steps based on the changes in the absorption spectrum (Fig 2B,C) In addition, picolinic acid was not detected in the reaction mixture after the coupled enzyme assay The other possibility ... The decarboxylation mechanism in the metabolic pathways for 3-hydroxyanthralinic acid differs from that in the pathway for 4-amino3-hydroxybenzoic acid The N-terminal amino acid sequence of the...
... wonder whether the donor allele of the 5¢ part is the F allele or another allele belonging to the same B allelic lineage (excluding B1 and C), as they share the same simplified haplotype formula, ... expected forthe F variant This result is the first evidence forthe heterozygous status (M/F) of the individual goat milk analysed In addition to this, the HPLC profile confirms that the M variant ... phosphorylation of Ser64 in the M variant The sequence determined is consistent with the molecular mass measured forthe native protein The phosphorylated residues are therefore Ser46, 48, 65, 67,...
... part is unique for GIRK1 among the Kir3.x isoforms Further truncations of the C-terminus revealed a 49 amino acid stretch (amino acids 362–411) that was the most prominent target for phosphorylation ... currents had been described previously [18] To assess the role of the S ⁄ Ts in the regulation of GIRK1 via PKA in a manner that is unbiased by the eventual contributions of the other subunits in ... incubation of cells for days at 19 °C, the oocytes were checked for expression with the two electrode voltage clamp technique as described below and the cells were injected with either SpcAMPS or...
... Molecular modeling of the 3D structure of the mSMOl isoform was thus carried out in order to test the hypothesis that the 277–307 region and the protein domain encoded by the exon VIa could be ... substantiate the hypothesis that these two structural regions are mandatory forthe nuclear localization of mSMOl, as the only difference between mSMOl and mSMOlD proteins consists of the lack of the ... Discussion In the murine polyamine homeostasis at least two catalytically active splice variants of the spermine oxidase enzyme are involved The cytosolic mSMOa is themajor isoform, while the mSMOl...
... from the administered [75Se]Der p2, indicate together with its own remaining intact levels (the 16 kDa band) that the clearance and further metabolism of the allergen was altered as a result of the ... At the earlier time points the lung showed the strongest radioactivity labelling The radioactivity decreased with time and at 48 h the radioactivity detected in lung was approximately of the ... remaining in the trachea demonstrates that the model is suitable for studies of inhaled allergens The i.t instillation route was used to minimize the loss of [75Se]Der p during the exposure Although...
... element form double helical structures This robust conformation is maintained at 22 °C as well as 37 °C and may be necessary forthe function of the CU-element, e.g for protein binding Therefore, the ... as the 52 nt CU element and therefore is responsibleforthe faint formation of complexes C1 and C2 with construct pDC5 (Fig 2B, lane 6) We did not detect any protein binding sequences within the ... showing the complex formation was only inhibited by the addition of increasing amounts of unlabeled 52 nt sense transcripts (lane 4–9) To identify proteins responsibleforthe formation of the two...
... carD alleles in the carS mutant SF134 and the four transformants described in the text The picture shows the electrophoretic separation of PCR amplification products obtained with the forward and ... [15,16], and derepressed in the dark in the carS mutants, which exhibit a deep orange pigmentation irrespective of the culture conditions [15,17] The genes needed forthe synthesis of b-carotene and ... Recently, we identified in this fungus the gene ylo-1 [24], which is responsibleforthe aldehyde oxidation step in neurosporaxanthin formation, and showed the ability of the encoded enzyme to convert...
... based on the binding affinity forthe K48-linked ubiquitin chains N1, N10 and ⁄ or N13 (for RPN1, -10 and -13, respectively) in bold and parentheses are the docking subunits for either the RAD23 ... critical forthe interaction S5a in parentheses is the designated name forthe human RPN10 homolog d IF, the interface regions on the UBL–UBA factors that are involved in binding with the docking ... patches within the UBLs of the DSK2 homologs for their involvement in the interaction with RPN13 The potential involvement of the hydrophobic patch in the UBL of hHR23b was also examined The UBL variants...
... To ascertain whether the presence of a negative domain in the N-terminal region of the pig enzyme could be responsiblefor its low level of malonyl-CoA inhibition, we determined the IC50 of wild-type ... enzymes The high degree of identity in the N-terminal sequences of these two proteins (Fig 3A) suggests that the docking of the N-terminal fragment into the C-terminal region is different between the ... not face the C-terminal fragment A possible explanation for this is that, in the case of CPT1B, the docking of the N-terminal fragment might differ from that of the established model A further explanation...
... of the total zinc in the ovary at stage and 13% of the total zinc in the testis at stage is still bound to MYP On the basis of the results of the present study and others, we propose a model for ... of zinc from the digestive tract to the gonad, forthe following three reasons First, most of the zinc in the coelomic fluid is bound to CFMYP (Fig 1) Second, CFMYP injected into the coelomic ... part of the zinc in the immature gonads is bound to MYP The elution patterns of zinc were similar in the ovary and testis, but the zinc peak in the immature ovary was larger than that in the immature...
... sequence, the amino acid combinations in these positions were major determinants forthe susceptibility of the protein to protein N-myristoylation For postgenomic studies, reliable tools forthe prediction ... permitted for rabbit NMT (Fig 3) Forthe amino acid at position 3, His is not permitted for S cerevisiae NMT1p [5], but was permitted for rabbit NMT (Fig 5A) Therefore, it seems likely that the restrictions ... [34] The Nmt1p fold consists of a saddle-shaped b-sheet flanked by a helices There is pseudo-2-fold symmetry The N-terminal half forms the myristoyl-CoAbinding site The C-terminal half forms the...
... L Robbel et al of the cyclic product [9] Furthermore, the rigidification of the molecule reduces structural flexibility, leads to the conformation required for interaction with the corresponding ... within the biosynthetic operon proposed to be responsiblefor disulfide formation [35] This oxidoreductase, although lacking in the gene cluster enabling thiocoraline biosynthesis, could carry out the ... enable the specific angle, following the Burgi–Dunitz trajectory, required ¨ forthe nucleophilic attack onto the acyl-O-TE oxoester intermediate [36] Furthermore, the correct positioning of the...
... shown The conformational movements of Ile371, located in the activation loop at the N-terminus of the catalytic domain, are notable In the zymogen FXI, Ile371 is exposed to the solvent, while in the ... contrast to the activated FXI, in the structure of the FXI zymogen, Val371 is located on a loop region, exposed to solvent, and does not form many contacts with other residues (Fig 6) Therefore, the ... stabilizing the active state (e.g Asp189, which is part of the S1 pocket responsibleforthe binding specicity of the substrate) [26] Consequently, substitution of Val371 to isoleucine might prevent the...
... passed over the surface of the chip The PfFabG immobilized surface devoid of the PfACP from a previous reaction could be regenerated by passing the buffer alone A typical sensorgram forthe binding ... experiments shows the involvement of these residues in the PfFabG–PfACP interaction Allosteric binding of NADPH to PfFabG mutants in the presence of PfACP The Ka value forthe binding of NADPH ... instead of Arg187, so the effects of mutations of these residues on the kinetics and the ACP-induced cooperativity forthe binding of NADPH were also examined To eliminate the ability of Arg187,...
... al CII by CIII works at the protease level, rather than at the substrate level Nevertheless, competition between CII and CIII for interaction with HflB also influences the proteolysis of CII [11], ... grown for a further 20 and poured into cold tubes All subsequent steps were performed at °C After centrifugation at 1900 g for 10 min, the cell FEBS Journal 275 (2008) 4767–4772 ª 2008 The Authors ... partially protected in the presence of CIII, and 65% of r32 remained undigested after 80 min, compared with 40% forthe control (r32 alone, Fig 2C) The inhibitory action of CIIIC on the proteolysis...
... centrifugation, the organic phase was isolated and the remaining lipids were further extracted by the addition of mL of chloroform to the aqueous phase and by shaking (in the presence of the glass beads) The ... in the de novo synthesis of a given phospholipid, including PI Hence, the rate of synthesis and the amount of CDP-DAG molecules that were used as substrate for PI synthesis appeared to be the ... novo synthesis, it might be involved in the stearic acid incorporation after the de novo synthesis of this lipid (i.e the second hypothesis) According to this hypothesis, previously raised for mammals...