... 66(1):167-167. 38 . Smith KR. The role of sperm-mediated gene transfer in genome mutation and evolution. Med Hypotheses 2002; 59(4): 433 - 437 . 39 . Zani M, Lavitrano M, French D, Lulli V. The mechanism of ... contributed to the embryo, the resultant offspring will be chimeric (up to ca. 50% of animals). The ESC contribution to a mouse can high (up to ca. 80% ofthe cells), and will often include the germline ... prospects for gene therapy. Gene Ther. 2002; 9(11):7 13- 718. 85. Lipps HJ, Jenke ACW, Nehlsen K, et al. Chromosome-based vectors for gene therapy. Gene Ther. 20 03; 30 4: 23- 33. 86. Abbondanzo...
... Saini J.S. and Solanki S.C. Heattransferand friction in solar air heater duct with V-shaped rib roughness on absorber plate. Int. Journal ofHeatand Mass Transfer 2002; 45: 33 83- 33 96. [19] ... S.J. The effect of gap position in discrete ribs on local heat/ mass transfer in a square duct Journal of Enhanced heattransfer 20 03; 10 (3) : 287 -30 0. [ 13] Gupta D. Solanki S.C and Saini J.S. Thermo-hydraulic ... value of turbulent intensity is observed at the relative gap width of 1.0 which is in the line ofheat transfer measurements. These results of PIV showed good agreement between theheat transfer...
... increase)Caspase -3 activity (fold increase)50 30 20100 3 42.5 3. 5 3. 5 3 2.521.510.500.5 mmol·L–1 H2O221.510.50CtrlCtrlpcDNA3.1pcDNA3.1-C 23 pcDNA3.1-Hsp70pcDNA3.1-C 23 + pcDNA3.1-Hsp70pcDNA3.1 ... lost.Taken together, our results demonstrate an impor-tant relationship among the regulation of nucleo-lin ⁄ C 23, the activation of caspase -3 andthe induction of apoptosis under the setting of oxidative ... minimize the number of animals used and their suffering.H2O2pcDNA3.1100100 0.5Time (h)1 23 Bcl-2 mRNA/GAPDH mRNA (%)pcDNA3.1 pcDNA3.1-C 23 Bcl-2GAPDHABFig. 5. Effect of nucleolin ⁄ C23...
... new mutants of the 3 -end of minus-strand RNA.ResultsEffect of mutations in the3 - or the 5¢-end of the (–)IRES HCV RNA template on RNA synthesis The secondary structure ofthe3 -terminal ... RNA synthesisby recombinant HCV NS5B using deletion mutants of the 3 terminus ofthe minus-strand RNA but deletionswere made on the basis ofthe structure ofthe 5¢UTR of the plus-RNA, the structure ... further information on the replication mechanism of the hepatitis C virus (HCV), we have studied the role of sequences at the 3 -end of HCV minus-strand RNA in the initiation of synthesis of the viral...
... detection of proteins crosslinkedFig. 2. Formation of two major complexes between Swiss 3T3 proteins and the3 -UTR ofthe MARCKS mRNA. (A) The MARCKS 3 -UTR, the stop codon UAA ofthe coding ... (CDS) and MARCKS 3 -UTR were drivenby a CMV-promoter and used for transfection of Swiss 3T3 fibro-blasts. Length ofthe MARCKS 3 -UTR sequences [38 ] of pDK1: 131 0–2597 bp (complete 3 -UTR of MARCKS); ... 2002)Eur. J. Biochem. 270, 35 0 36 5 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 033 96.xNP-40, 10 mMNaCl, 3 mMMgCl2 and 10 mMTris/HClpH 7.4) prior resuspending the nuclear pellet in glycerolstorage...
... C-terminal tyrosine of a-tubulin by 3- nitrotyrosine alters the ability ofthe molecule to formmicrotubules. For this purpose, we studied the behavior of nitrotyrosinated tubulin (and of tyrosinated ... D and G vs. H;Fig. 5B); (b) the normal appearance and proliferation of cells subjected to cycles of growth and passage for45 days in the continuous presence of 500 lMnitrotyro-sine; (c) the ... present in the cell.During the experiment shown in Fig. 3, number and viability of cells were determined every day. The number of cells increased during the first two days and then remainedconstant...
... Homology model ofthe subunit of 3- ureido-propionase, based upon the crystal structure of the D. melanogaster 3- ureidopropionase (Protein DataBank code: 2VHH).Fig. S3. To determine whether 3- ureidopropionasedeficiency ... dithiothreitol at)80 °C. The purity ofthe recombinat protein was ‡ 85% asjudged by SDS ⁄ PAGE.Molecular size ofthe 3- ureidopropionase The molecular size and oligomeric state ofthe 3- ureidopro-pionase ... fusionprotein under the control ofthe 3- ureidopropionasepromoter.Results and DiscussionCloning and expression of recombinant 3- ureidopropionase The sole homolog of 3- ureidopropionase in...
... aB-crystallin (i.e. the effect of the additive on the aggregation ofthe target protein is inde-pendent and different from that ofthe chaperone). The standard errors associated with this theoretical ... increased the chaperone activity of aB-crystallin(84 ± 4%) against this target protein. The effect of Arg-HCl on the structure and assembly of aB-crystallinWe investigated whether the effects of these ... further enhanced by it (Fig. 1C). The sig-nificant decrease in the amount of precipitation in the presence of high concentrations of Arg-HClin the absence ofthe chaperone precluded analysis of the...
... Terminology 131 C. Literature Review 133 1. Studies of children and adolescents 133 2. Premenopausal women 135 3. Perimenopause 137 4. Postmenopausal women 137 5. Physical activity and prevention of falls ... The Well-being of Canadians. Canadian Fitness and LifestyleResearch Institute: Ottawa. 33 . Dyck, L. (1998). The exercise of daily life: Towards an understanding ofthe meaning and practice of ... researchers expand the definition of health to include the social determinants of health. In a recentarticle on women’s health andthe contribution of physiotherapists, McComas and Harris [38 ] use...
... about the regulation ofthe polymer at the genetic level of itssynthesis and degradation. The gene for polymalatasehas been sequenced (accession number AJ5 433 20 [14]), and distinct features of ... ([6] and references therein), suggesting the presence of detect-able amounts of PMLA synthetase-specific mRNA. The absence of PMLA and polymalatase in other celltypes could be the result of cell ... 3aby 14 amino acid substitutions, eight of them locatedin the first two b-strands ofthe N-terminal half of NKA48, upstream ofthe homodimer interface and accessible for interactions with other...