derivation of epidermal colony forming progenitors from embryonic stem cell cultures pdf
Dual roles of transcription factor zic3 in regulating embryonic stem cell pluripotency and differentiation
... derivation of five human ES cell lines by Thomson et al from the B ES cells A Embryonic Development Figure Contribution of the blastocyst inner cell mass to embryonic development and embryonic stem cells ... understanding of the role of Zic3 in development ix LIST OF FIGURES Figure Contribution of the blastocyst inner cell mass to embryonic development and embryonic stem cells Figure Components of the ... saline xiv CHAPTER 1: INTRODUCTION 1.1 Derivation of embryonic stem cells The inner cell mass (ICM) of an embryonic blastocyst is a source of pluripotent cells that ultimately give rise to the...
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Báo cáo khoa học: "The Derivation of a GrammaticaUy Indexed Lexicon from the Longman Dictionary of Contemporary English" pptx
... representation of the lexicalentry for a particular word As an illnstmtion of the process of transforming a dictionary entry into a lexical template we show below the mapping of the third verb sense of %elieve" ... logical type of an E q u i verb In the course of an interactive cycle of development, a number of entries are hypothesised and automatically generated from x single base form The family of related ... predictable on the basis of the part of speech of the item involved, those which follow from the inflectional or deriv~tional morphological rules incorporated into the system, ~nd those which rely...
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Báo cáo y học: "A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes." ppsx
... CD: Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency Stem Cells 2005, 23:663-680 35 Munne S, Howles CM, Wells D: The role of preimplantation ... Page of 18 onic stem (mES) cell clones capable of the inducible overexpression of each one of 32 selected genes, 29 murine orthologs of HSA21 genes and HSA21 coding sequences, under the control of ... overexpression of some of the HSA21 genes is due to a high basal expression level of these genes Dosage sensitivity of HSA21 genes in mES cells We further investigated the cause of the lack of a strong...
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Báo cáo y học: " Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development" ppt
... differentiation of embryonic stem cells Curr Opin Cell Biol 1995, 7:862-869 10 Dani C, Smith AG, Dessolin S, Leroy P, Staccini L, Villageois P, Darimont C, Ailhaud G: Differentiation of embryonic stem cells ... the mESC culture system might not only allow the generation of adipose stem cells, but also of their potential vascular niche, opens new avenues for the study of adipose stem cell biology and their ... Wdziekonski B, Villageois P, Dani C: Differentiation of mouse embryonic stem cells and of human adult stem cells into adipocytes Curr Protoc Cell Biol 2007, Chapter 23(Unit 23 24) 54 Irizarry RA,...
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Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx
... methylation profile of the human embryonic stem cell line HSF1 [22] BS-seq is able to generate genome-wide DNA methylation profiles at single base resolution, much improved from previous profiling ... changes of methylation levels between pairs of cell lines at binding sites with the changes of the expression levels of their associated genes The coordinates of TFBSs were downloaded from Motifmap ... for providing embryonic stem cell DNA (HSF1) and discussion P-YC is funded by Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA SF is Special Fellow of the Leukemia...
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Báo cáo y học: "Role of epidermal growth factor receptor activation in regulating mucin synthesis" pdf
... new goblet cells were produced by direct conversion of Clara cells Finally, EGFR expression and activation causes goblet -cell metaplasia without changing the total number of epithelial cells, indicating ... number of goblet cells increases and the number of Clara cells decreases equivalently, thus implicating a differentiation process in goblet -cell development [4] Stimuli for mucin synthesis: role of ... epidermal growth factor receptor cascade The mechanism of EGFR expression and activation Stimulation of airway epithelial cells with TNF-α causes EGFR expression (the extracellular and intracellular...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of ... structures of the peripheral nervous system, such as Schwann and glia cells of the autonomic and enteric nervous systems, endocrine cells, such as the adrenal medulla, and C-cells of the thyroid ... of gastrulation Lack of Eomes abrogates the formation of embryonic and extra -embryonic mesoderm [42] It has been shown that Eomes is specifically required for the directed movement of cells from...
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Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
... using an in vitro differentiation system of mouse ESCs Replating of embryonic bodies (EBs) of mouse ESCs resulted in the formation of blast colony forming cells (BL-CFCs), which possessed hemangioblastic ... derived from human embryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Lu S-J, Li F, Vida L, Honig GR: CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit ... hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell- derived NK cells acquire functional receptors...
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Báo cáo y học: "Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes" doc
... interactions Enrichment Figure of α-MHC+ cells isolated from the α-MHC+ ES cell lineage after puromycin treatment Enrichment of α-MHC+ cells isolated from the α-MHC+ ES cell lineage after puromycin ... Hescheler J, Sachinidis A: Embryonic stem cells: a promising tool for cell replacement therapy J Cell Mol Med 2004, 8:465-473 Winkler J, Hescheler J, Sachinidis A: Embryonic stem cells for basic research ... lists of transcripts (a) The pie chart shows the distribution of these annotations (b) The bar chart shows the number of genes in the categories adhesion, cell cycle, cell death, cell- cell signalling,...
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Báo cáo y học: "nalysis of the mouse embryonic stem cell regulatory networks obtained by ChIP-chip and ChIP-PET" pptx
... range of threshold values Embryonic stem (ES) cells are derived from the inner cell mass of the embryo and possess the property of pluripotency, which is the ability to develop into any cell lineage ... circuitry in human embryonic stem cells Cell 2005, 122:947-956 Kim J, Chu J, Shen X, Wang J, Orkin SH: An extended transcriptional network for pluripotency of embryonic stem cells Cell 2008, 132:1049-1061 ... which a stem cell maintains its identity, and how it can be directed to form different cell types Our work will allow for a more detailed examination of the components of this expanded stem cell...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... MEF Mouse embryonic fibroblast xiv Chapter I – Introduction 1.1 Transcriptional control of stem cell pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, ... regional specification of midbrain by addition of the right concentrations of SHH and FGF8, several groups reported the efficient generation of dopamine neurons from embryonic stem cells (Cho et al., ... Figure 1.3 Patterning of the neural tube generates unique domains for neuronal progenitors 13 Figure 1.4 The differentiation of pluripotent stem cells into neuroepithelial stem cells and radial glia...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
... resulted in loss of OCT4 The percentage of OCT4+ cells from Figure 6.7 was quantified by flow cytometry The number of OCT4+ cells were significantly reduced upon knockdown of any of the three pluripotent ... knockdown of neuronal lncRNAs The percentage of TUJ1+ cells from Figure 7.6 was quantified by flow cytometry The number of TUJ1+ cells were significantly reduced upon knockdown of any of the four ... networks The enriched and highly homogenous cultures of neural progenitors and neurons derived from hESCs provided an ideal source of cells for expression profiling to identify lncRNAs that are necessary...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
... ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas (PFSK-1) indicated a REST binding peak upstream of RMST, suggesting that REST may regulate the transcription of RMST (Figure ... factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST chromatin-immunoprecipitation ... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
... of pluripotent stem cells from adult human fibroblasts by defined factors Cell 131, 861-872 Takahashi, K., and Yamanaka, S (2006) Induction of pluripotent stem cells from mouse embryonic and adult ... E.R (2009) Isolation and characterization of neural crest stem cells derived from in vitro-differentiated human embryonic stem cells Stem Cells Dev 18, 1059-1070 Johnson, R., Teh, C.H., Jia, H., ... of human embryonic stem cells induced by stromal cells Stem Cells Dev 19, 71-82 Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S (2007) Induction of...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
... Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage embryos, offer a renewable and unlimited source of ... HUMAN EMBRYONIC STEM CELL- DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY (B.Sc (Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... individualized cellular therapies While the promise of iPS cell technology is great, there are challenges to overcome for their clinical application, such as the lengthy derivation of iPS cells, cell- intrinsic...
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Establishment of autologous culture systems for human embryonic stem cells
... their derivation sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem ... pluripotent cell source for future clinical applications Literature review Table Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells ... Human embryonic stem cells (hESCs) Stem cell is a distinct type of cell that can renew itself and give rise to multiple specialized cell types [National institutes of health (NIH) stem cells...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... and expansion of neuroprogenitors, which resulted in an increased yield of dopaminergic neurons derived from the neuroprogenitors Transcriptional profiling of overexpressing SHH neuroprogenitors ... Background Human embryonic stem cells (hESC) are a widely envisioned source of cells for use in cell replacement therapy In particular, medical conditions arising from the loss of neurons, like...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
... seeding densities of 300 and 500 cells per 56cm2 (surface area of each well of a well dish is 56cm2) When 300 cells were reseeded, the number of secondary colonies formed for cells transfected ... cells Put together, all these evidence suggest that the regulation of Lefty1 by OCT4, KLF4 and NANOG may be part of the ES cell self renewal mechanism The role of Lefty1 in regulation of ES cell ... Figure Partial loss of Lefty2 led to reduction of Nanog protein expression in LIF supplemented E14 mouse ES cell cultures, as observed with immunofluorescence microscopy Cell nuclei were stained...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3
... embryonic development and stem cell- specific expression of Lefty has been observed in mouse and human, its functional significance in embryonic stem cells has never been demonstrated A part of ... role(s) in embryonic stem cells To elucidate the biological role(s) of Lefty2, loss of function studies using shRNAs was carried out I was able to show that Lefty2 RNAi induces ES cell differentiation ... specification of ES cells is a SMAD3 dependent process On the other hand, SMAD3 plays no role in trophectodermal 158 specification of ES cells as inhibition of SMAD3 activity does not lead to ES cell...
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