derivation and invasive function of trophoblast from human pluripotent stem cells
Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... differentiation and tissue engineering, and the challenges that need to be overcome before clinical application which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells ... application of ESCs is further advanced with the development of embryonic-like stem cells, also known as induced pluripotent stem cells (iPSCs), as a potential autologous pluripotent stem cell ... and cartilage degeneration after a temporary relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... expanded cells as a mixture of ECs and MCs derived from human ES cells (hESECs+MCs) The cell mixture was composed of almost the same number of ECs and MCs We resorted the VE-cadherin+ cells from ... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of ... muscle actin; hESECs+MCs: a mixture of ECs and MCs derived from human ES cells; hES-ECs: ECs derived from human ES cells; hESMCs: MCs derived from human ES cells http://www.translational-medicine.com/content/6/1/54...
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Atlas of Human Pluripotent Stem Cells doc
... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass blastocyst ... the derivation of ESC lines from mouse embryo and the derivation of human lines using basically the same culturing techniques M Amit and J Itskovitz-Eldor, Atlas of Human Pluripotent Stem Cells: ...
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Structure and function of methyltransferases from antibiotic resistance bacteroides of human intestine and a study on nm ng with cam
... of apo BT_2972 and structures of its complex with AdoMet and AdoHcy revealed significant conformational changes which resulted in open and closed forms to regulate the movement of cofactors and ... chromatography elution profile of BT_2972 (dotted line) and BVU_3255 (solid line) Elution profile of molecular weight standard is shown as dashed line and marked with different size of proteins (kDa) ... Structure of BT_2972 (A) Ribbon representation of the crystal structure of BT_2972-AdoMet complex Stereo view of 2Fo-Fc map of (B) AdoMet and (C) AdoHcy in BT_2972 complexes 41-42 Figure 3.3: ITC profiles...
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Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf
... sm The points from N-terminal and C-terminal residues (Arg1–Leu7 and Ala60–Gln67), together with those from Arg58 and Gln59 in the C-terminal end of helix III, are located apart from the major ... of a hinge point at Arg53 that exhibits conspicuous minima of J(0) and J(xN) and a significant maximum of J(0.87xH), and that emerges not only within helix III, but also over a large portion of ... S1 Bar graph of R1, R2 and {1H}–15N NOE values along the sequence of the TTF1 HD at 11.7 T and 286 K 448 Fig S2 Plot of J(0.87xN)–J(0) correlation from 15N relaxation measurements of the TTF-1...
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báo cáo hóa học:" Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels" docx
... distribution of the ECs projection area was calculated HUAECs (Figure 4A, average of cells from 11 individuals from each group) and HUVECs (Figure 4B, average of cells from individuals from each group) from ... Characterization of the cell types and growth kinetics of cultured cells Healthy growing EC and SMC cultures were obtained from UC of group-1 and group-2 individuals No contamination of SMCs in EC ... area of human umbilical artery and vein endothelial cells grown to confluence Projection area of human umbilical artery and vein endothelial cells grown to confluence Passage 2–4 HUAECs and HUVECs...
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Báo cáo y học: "Apremilast, a novel PDE4 inhibitor, inhibits spontaneous production of tumour necrosis factor-alpha from human rheumatoid synovial cells and ameliorates experimental arthritis" docx
... concentration (IC50) of 74 nM and inhibits TNFα production from lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMC) and whole blood by 7.7 nM and 11 nM, respectively ... are representative of three experiments from different donors, all of which displayed a similar dose-dependent inhibition of Page of 11 TNFα by apremilast and rolipram Addition of DMSO alone (vehicle ... concentrations of apremilast and rolipram ranging from 6.25 nM to 100 nM As a positive control, cells were treated with a combination of anti-TNFα mAb and IL-1RA (both at 10 μg/ml; R&D Systems, Greater...
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Báo cáo y học: " Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy" pps
... Figure of macrophages from lentiviral vector transduced and normal hES cells Derivation Derivation of macrophages from lentiviral vector transduced and normal hES cells A) Transduced and non-transduced ... Results Derivation of macrophages from hES cells Undifferentiated hES cell colonies grown in media supplemented with ng/ml bFGF displayed normal morphology of pluripotent human embryonic stem cells ... undifferentiated state [5-7] Thus, these cells provide a renewable source of pluripotent stem cells from which many types of differentiated cells could be produced for experimental and therapeutic purposes Cell...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... categories of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their ... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of pluripotent ... somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells and neuronal stem cells Adult stem cells have...
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ANALYSIS FOR STRUCTURE AND FUNCTION OF AN ECOSYSTEM HUMAN IMPACTS, CONSEQUENCES AND SOLUTIONS
... Location Biotics and abiotics Energy and material flow Human impacts, consequences and solutions Conclusion LOCATION Thong Nhat Park Peninsula : 1200 m2 Including : mainland, lake, plants, ... restaurant in this area and gases from vehicles on Le Duan road HUMAN IMPACTS - CONSEQUENCES SOLUTIONS Increasing the number of wastebasket Planning the rubbish dumps and quick transporting ... restaurant and visitors to keep the park green, clean and beautiful CONCLUSIONS Plant species on peninsula are quite diverse People are having huge impacts on the formation and development of the...
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Báo cáo y học: " Derivation and preliminary validation of an administrative claims-based algorithm for the effectiveness of medications for rheumatoid arthritis"
... authors made substantial contributions to conception and design, and to the analysis and interpretation of the data TRM and GWC handled acquisition of data All authors contributed to the manuscript ... the gold standard criteria were, the most common reasons were an increase in the dose of oral glucocorticoids and addition of new non-biologic DMARDs The extent of bias resulting from misclassification ... in the population, and more reflective of the test itself, lessening the impact of any unique features of the VA population Moreover, we might expect that the PPV and NPV of the algorithm might...
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Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf
... Isolation of mononuclear cells and granulocytes ¨ from human blood Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at g Scand J ... 2003 Structure and function of chlamydial lipopolysaccharide (Eur J Biochem 270) 449 Table Comparison of LPS-induced IL-8 release of HEK-vec and HEK-CD14 cells HEK-vec or HEK-CD14 cells were transiently ... CSD ESI-FT-ICR mass spectrum of de-O-acylated LPS from Fig Negative ion MALDI-TOF mass spectra of lipid A isolated from C trachomatis serotype E (A) and L2 (B) and from Chl psittaci 6BC (C) C...
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Báo cáo khoa học: Identification and functional expression of a second human b-galactoside a2,6-sialyltransferase, ST6Gal II docx
... human ST6Gal II (Eur J Biochem 270) 953 Fig Nucleotide and predicted amino acid sequence (A) and hydropathy profile (B) of human ST6Gal II, and (C) comparison of the sialylmotifs L, S and VS of ... (nucleotide 1–35, Fig 1A) and the 3¢ primer described above The 25 lL of PCR mixture consisted of unit of DyNazyme EXT, 0.3 lM of each primer, 0.2 mM dNTP and 1.5 ng of plasmid DNA Reactions were ... to homogeneity from animal livers and hepatoma cells (reviewed in [3]) and cDNA has been cloned from rat liver [21], human placenta [22], mouse liver [23], bovine tissues [20] and chick embryo...
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Báo cáo khoa học: Purification, characterization and molecular cloning of tyrosinase from the cephalopod mollusk, Illex argentinus docx
... dialyzed solution of AS60 (300 mL) obtained from 100 g of defatted ink powder contained 2.9 g of protein, 0.29 g of hexose, 0.25 g of uronic acid, 0.12 g of methylpentose and 5400 units of tyrosinase ... cysteine-histidine thioether bridge in functional units of molluscan haemocyanins and location of the disulfide bridges in functional units d and g of the bc-haemocyanin of Helix pomatia Eur J Biochem ... molecular masses of the subunits of ST94 and ST94t were similar to those of the proenzymes ( 70–80 kDa) and the activated enzymes by proteolysis ( 60–70 kDa) of a bivalve tyrosinase [34] and arthropod...
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Báo cáo khoa học: Stabilities and activities of the N- and C-domains of FKBP22 from a psychrotrophic bacterium overproduced in Escherichia coli pptx
... composed of N- and C-domains, which are spanned by a 40 amino acid long a3 helix The N-domain consists of a1 and a2 helices and an N-terminal region of a3 helix The C-domain consists of six b-strands ... contrast, the spectrum of C-domain– was quite different from those of C-domain+ and SIB1 FKBP22*, suggesting that the structure of C-domain– is considerably different from that of the C-domain in ... Comparison of thermal stability of SIB1 FKBP22* and E coli FKBP22* Fig DSC curves of N-domain+, C-domain+, and SIB1 FKBP22* The DSC curves of N-domain+ (thick line), C-domain+ (thin line), and SIB1...
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the idea of idolatry and the emergence of islam from polemic to history
... Bakka and in which was the standing place (maqam, i.e., place of prayer) of Abraham; 2:125 again refers to the standing place of Abraham and to the house which Abraham and Ishmael were commanded ... those of Moses, Jesus and other prophets, preserved it intact and in its pristine form (This understanding of the value to Islam of its own account of its origins is supposition: I not know of any ... of their own version of the common tradition Islam could be presented as a version of the truth adapted to the needs of pagan Arabs and bearing within it some of the marks of the idolatrous and...
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Outline môn Tiếng Anh - What are the good and bad points of advertising from the view point of a consumer?
... THANH 1102015096 Contents Advertising can inform people about different products and services and their utilities, cost and other requirements It helps consumers know what is the best option for them ... STRENGTHS They can supply a wide range of products to their customers because customers watch television many times a day so they can easily recognize brand name of the suppliers The customers are ... 13 By what means can a product or a servicebe advertised? Briefly present the strengths and weaknesses of each means A product or a service can be advertised by many means such as: Television,...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB, and between hES cells and adult cells was significant ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, ... expression profiling We assessed global hES gene expression profiles on separate passages of cells from different hES cell lines, EB samples at different time points, and types of adult cells, HUVEC,...
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báo cáo hóa học:" Gene and microRNA analysis of neutrophils from patients with polycythemia vera and essential thrombocytosis: down-regulation of micro RNA-1 and -133a" pot
... Comparison of the expression of CD15, CD18, and CD177 by neutrophils from MPD patients, healthy subjects, and healthy Comparison of the expression of CD15, CD18, and CD177 by neutrophils from MPD ... comparison of normal pancreas with 33 other tissues found that the expression of miR-216 and miR-217 and the lack of expression of miR-133a were characteristic of pancreatic tissue [36] Page of 17 ... of JAK2 V617F and the development of MPD [1,9] Further characterization of the molecular changes in MPD neutrophils could lead to a better understanding of the development of these diseases and...
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Báo cáo hóa học: " Shape-tailoring and catalytic function of anisotropic gold nanostructures" doc
... carried out the reduction reaction of 4-nitrophenol by NaBH4 Figure Nanoflower and satellite-like structures of AuNPs (a) “Nanoflower” and (b, c) “satellite structures” of AuNPs Premkumar et al Nanoscale ... the Ministry of Education, Science and Technology (MEST) of Korea (Project no R31-10026) Author details Department of Materials Science and Engineering, Gwangju Institute of Science and Technology ... the formation of nanoparticles were studied systematically Final concentrations of the metal ion and detergent in each sample (expressed as the millimolar concentration of metal ion and weight percent...
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