derivation and characterization of gut like structures from embryonic stem cells pdf
The role of kruppel like factors in embryonic stem cells
... PUBLICATIONS LIST OF ABBREVIATIONS CHAPTER I INTRODUCTION 1.1 Pluripotent stem cells 1.1.1 Mouse embryonic carcinoma cells 1.1.2 Mouse embryonic stem cells 1.1.3 Mouse embryonic germ cells and pluripotent ... spermatogonial stem cells or adult germline stem cells have been derived from spermatogonial cells of adult mouse and human testis These cells are able to differentiate into various types of specialized cells ... potentials: totipotent stem cells, pluripotent stem cells, multipotent stem cells, and unipotent stem cells A totipotent cell can develop into a whole organism Pluripotent cells can give rise to...
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Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf
... cells and purified from the soluble fraction of bacterial cells as described in Experimental procedures The yield of C49S was the same of that obtained for the wild-type protein The activity of C49S ... activity of Bcp2 in S solfataricus Fig Multiple sequence alignment (CLUSTAL W 1.82) of Bcp2 from S solfataricus and Prxs from A pernix (APE2278), T maritima (Q9WZR4), P horikoshii (PH1217), and human ... range of concentrations (0– 100 lgÆmL)1) (B) Effect on protection against DNA cleavage of the replacement of Cys49 of rBcp2 with serine Lane 1, pUC19; lane 2, pUC19 and 10 mM DTT; lane 3, pUC19 and...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... 205 and 215 nm, indicating that Rv1399c is composed of 22% of a-helices, 25% of b-strand and 39% of random coil The catalytic triad is located at the bottom of a small ˚ ˚ pocket, 20 A length and ... consists of three domains The largest domain encompasses strands b1 to b6 and strand b7 to the C-terminus The central b sheet is composed of parallel b strands associated to an antiparallel strand ... obtained from the pDest 17O/I construct using the BL21 (DE3)pLysS cells grew in LB medium after h of IPTG induction (Fig 1) Purification and refolding of Rv1399c Given the large quantity of insoluble...
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Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
... were purchased from Sigma Chemical Co DEAE-Toyopearl 650 M and Butyl-Toyopearl 650 M were from Tosoh (Tokyo, Japan) FPLC-Mono Q was from Pharmacia Substrates and standards were from commercial ... New BioLabs and Gibco BRL Pfu DNA polymerase and alkaline phosphatase were from Promega and Boehringer Mannheim, respectively D-Amino acid oxidase (EC 1.4.3.3) from porcine kidney and horseradish ... promoter and lacq repressor of pTrcHis2A, isopropyl thio-b-D-galactoside was added to a final concentration of mM Materials, enzymes and chemicals Restriction enzymes and T4 DNA ligase were from New...
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Báo cáo khoa học: Genomic structure, expression and characterization of a STAT5 homologue from pufferfish (Tetraodon fluviatilis) ppt
... isolation and characterization of the STAT5 gene from T fluviatilis (TfSTAT5), the third member of the STAT family from fish, to establish the evolutionary relationship of STAT5 between mammals and teleosts ... The sequences of the upper strand of the normal and the corresponding mutant oligonucleotides are listed in Table The probes were prepared by annealing the upper and lower strands of oligonucleotides, ... structure of TfSTAT5, the full-length cDNA was synthesized by PCR amplification using first-strand cDNA prepared from T fluviatilis gill and specific primers designed from the results of 5 and 3¢-RACE...
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Báo cáo hóa học: " Detection and characterization of chicken anemia virus from commercial broiler breeder chickens" pdf
... In the present study, out of 420 organ samples tested, 75% of spleen, 68.3% of bone marrow, 70% of thymus, 53.3% of liver, 46.7% of duodenum, 66.7% of ovary and 48.3% of oviduct tested were positive ... maximum homology with isolates from China (NF3A and PYT4), isolate A2 from Japan (NF1D and NF2C) and CAV-B isolate from India (PPW4) Only two of the isolates (M1B1 and NF3A) were found to have ... report detection of CAV and characterization of isolates based on sequence and phylogenetic analysis of partial VP1 gene from commercial broiler breeder chickens in Malaysia Level of transmission...
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Báo cáo hóa học: " Identification and characterization of alkaline serine protease from goat skin surface metagenome" docx
... mM EDTA, 1.5 M NaCl), in the presence of 0.1 mg of proteinase K and mg of lysozyme and incubated at 37°C for 30 Lysis was completed by adding ml of 20% SDS and incubated for h at 65°C with shaking ... 65 and 85% of the maximum activity at the temperature range of 35°C and 55°C respectively Thermal stability of the purified AS-protease was estimated at Figure Multiple sequence alignment of ... identification of protease genes from metagenomic library could be attributed to the problems associated with the expression of cloned gene in the heterologous host (Handelsman 2004) and low frequency of...
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Báo cáo hóa học: " Preparation and characterization of spindle-like Fe3O4 mesoporous nanoparticles" pot
... the arrangement of cations produces pairs of Figure Magnetic hysteresis loops of a-Fe2O3 NPs (curve 1) and Fe3O4 NPs (curve 2) (a); photographs of a-Fe2O3 NPs and Fe3O4 NPs before and after magnetic ... transformation of NPs from a-Fe O to Fe O Moreover, the detection of the phosphate reveals that the phosphate possibly plays an important role in the formation of the spindle and porous structures ... thus = M , and M = As · S, where r is the V density of the sample; M, S and V are the mass, surface area and volume of one Fe3O4 particle, respectively; As is the BET surface area of the sample...
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Báo cáo hóa học: " Preparation and Characterization of Nano structured Materials from Fly Ash: A Waste from Thermal Power Stations, by High Energy Ball Milling" potx
... photomicrographs of fresh and modified fly ash (A and B) SEM of fresh fly ash at 1,000 and 2,500 times magnification, (C and D) SEM of ball milled fly ash for 20 h at 1,000 and 3,000 times magnification, (E and ... F) SEM of ball milled fly ash for 40 h at 1,000 and 3,000 times magnification, (G and H) SEM of ball milled fly ash for 60 h at 1,000 and 3,000 times magnification Fig 10 (A and B) TEM images of fly ... Table Composition of the fresh fly ash (Class F) cl þ cl ð2Þ Subscripts s and l represent solid and liquid states respectively and h represents the contact angle of the liquid and the material...
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Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc
... citatsomreht a ot detcennoc )nedewS ,aicamrahP( tinu IIrohpitluM eht ni demrofrep saw sisylana citerohportcele ehT la te gnisucof cirtceleosi lacitylanA snoitacifidom thgils htiw ]42[ nibwoT fo dohtem ... ,BKL( rettolb yrd-imes tolBavoN a gnisu demrofrep saw sisylana tolbonummI la te gnittolbonummI ]31[ ilmmeaL yb debircsed snoitidnoc eht ot gnidrocca demrofrep saw siserohportcele dna ,selpmas ,sleg ... noitaraperP )ASU ,daRoiB( lleC balS-lauD II naetorP -iniM eht ni demrofrep saw sisylana citerohportcelE EGAP-SDS ]3[ dohtem drofdarB eht yb detamitse saw sPLG eht fo tnetnoc nietorp ehT fo sPLG...
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Báo cáo khoa học: "Expression and characterization of the UL31 protein from duck enteritis virus" pot
... threonine, and on tyrosine residues (Fig 1) Figure Prediction of Phosphorylation sites of deduced amid acids of the DEV UL31 protein Prediction of Phosphorylation sites of deduced amid acids of the ... Scientific and Technical Innovation Project, Ministry of Education of China (706050), the Cultivation Fund of the Key Scientific and Technical Innovation Project, department of Education of Sichuan ... located in the cells of immunological organs and digestive organs These properties of the UL31 protein provide a prerequisite for further functional analysis of this gene Results and discussion...
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Báo cáo y học: "Isolation and characterization of microparticles in sputum from cystic fibrosis patients" pot
... identification and characterization of MPs in the sputa of CF patients Patients and Methods Study patients The study was approved by, and performed in accordance with, the ethical standards of our institutional ... Identification of MPs by flow cytometry Representative dot plots and histograms of MPs from sputum from CF patients and P aeruginosa and S aureus MPs (A), P aeruginosa PAO1 (B), S aureus (C) and calibrator ... microscopy of MPs from the sputum of a CF patient Multiple spherical particles, ranging from 100 to 500 nm, are visualized Page of * A) 7.5 50 25 stable PCD CF B) Figure Quantification of MPs in CF and...
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Báo cáo sinh học: "Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus" pps
... aliquoted and stored at −80 ◦ C for subsequent screening by hybridization 2.2 Characterization of the BAC library BAC DNA was isolated from 100 colonies randomly chosen from each fraction of the ... 4, and until 100 generations had passed BAC DNA from each clone was prepared as above on day and NotI and HindIII restriction enzyme digestion patterns of BAC clones were compared at day and ... nanograms of DNA was used and the cycling conditions were as follow: 95 ◦ C, and 99 cycles of 95 ◦ C, 30 s; 50 ◦ C, 20 s; 60 ◦ C for RESULTS AND DISCUSSION A channel catfish BAC library was produced from...
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Báo cáo khoa học: Identification and characterization of novel PKA holoenzymes in human T lymphocytes pdf
... kDa band in T cells A Cb-specific antibody anti-Cb(SNO157) (vi) recognized the 47 kDa band in T -cells, and the 40- and 47 kDa protein bands in Jurkat cells (C) Linear depiction of Ca1, Cb1 and ... T cells and Jurkat cells and a 40 kDa protein band in Jurkat cells As no 40 kDa band could be detected using the anti-Cb(SNO157) in B C Fig Identification of PKA catalytic subunits in human T cells ... tissues such as spleen and thymus express Cb1 and Cb2 mRNA [6] Total RNA was isolated from human T cells, the T-cell line Jurkat and NT-2 cells, and amplified by RT ⁄ PCR using Cb1 and Cb2-specific primers...
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Báo cáo khoa học: Gene cloning, expression and characterization of avian cathelicidin orthologs, Cc-CATHs, fromCoturnix coturnix pdf
... phases of host defense, such as the induction of angiogenesis, the promotion of wound healing, and chemotaxis for neutrophils, monocytes, mast cells and T cells, as well as the inhibition of apoptosis ... Results Identification and characterization of quail cathelicidins Total RNA was extracted from the quail spleen On the basis of the end of the 5¢-UTR and the first 20 bp of the fowlicidin signal ... as the greater length of time CATH2 since they shared a common ancestor In addition, CATH1 and from C coturnix fall into one branch, and CATH1 and from Phasianus colchicus and chicken fowlicidins...
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báo cáo hóa học:" Synthesis and characterization of integrated layered nanocomposites for lithium ion batteries" pdf
... morphology and size distribution of the Li[NixCoxLi1/3-x-Mn2/3-x]O2 system were examined by FE-SEM and is shown in Figure From the SEM results, it is observed that the average particle size of the ... Li[NixCrxLi1/3-xMn2/3-x]O2 system x = 0.33 (113) (108) (110) The XRD profiles, ICP-AES results, SEM images, and electrochemical properties of Li[NixCrxLi1/3-xMn2/3-x]O2 system, where x = 0, 0.05, 0.1, 0.17, 0.24, and ... voltage profiles of such layered nanocomposites arise mainly from the oxygen loss occurring at extended charge cycling (> 4.5 V) [12,14] A maximum initial discharge and charge capacities of 270 and...
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Báo cáo y học: "HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall-derived mesenchymal stem cells" pot
... of stem cells, such as c-kit, BCRP-1, Notch-1, Sox-2 and BMI-1 (data not shown) To determine whether these cells also expressed the mRNAs of classical HIV receptor CD4 and co-receptor CXCR4 and ... smooth muscle and endothelial cells To study the effects of HIV-1 on the differentiation of these cells, the interaction of HIV-1 and recombinant gp120 on MSC differentiation to adipogenic and endothelial ... diffuse vascular damage and the promotion and development of atherosclerosis lesions due to impaired MSC repair control of endothelial and vessel structure On the other hand, HIV and gp120 induce the...
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