depreciation of long lived physical assets including infrastructure assets condition based depreciation and other related methods
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... 24 1.4.4 Other molecular functions of lncRNAs 25 Chapter II: Aims and Objectives ……………………………………………… 26 2.1 Main goals of the thesis 26 2.2 Thesis outline 27 Chapter III: Materials and Methods ... Feeder-free culture of human pluripotent stem cells 28 3.2 Expansion and mitotic inactivation of MEF cells 28 3.3 Preparation of MEF-conditioned medium 29 iii 3.4 Culture of PA6 mouse skull ... Differentiation of hESCs into neural progenitors and dopaminergic neurons 30 3.5.1 3.5.2 3.5.3 Co-culture of PA6 and hESCs for stromal-derived inducing activity (SDIA) Isolation and expansion of neural...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
... hESCs and NPCs (Figure 5.1) In addition, the expression profiles of these lncRNAs mirror that of known drivers of neuronal differentiation (Figures 5.2E-F), and were therefore indicative of important ... any of the three pluripotency lncRNAs resulted in a loss of OCT4 protein (Figure 6.7 and 6.8) and mRNA (Figure 6.9B) In addition, knockdown of lncRNAs resulted in downregulation of a panel of ... upstream of lncRNA_ES1 indicated that lncRNA_ES1 might be part of a longer transcript that has not been validated yet (B) lncRNA_ES2 is transcribed from the minus strand, and overlaps with another...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
... hnRNPA2B1 and SOX2 as protein partners of the lncRNA Two additional protein bands corresponding to 37 kDa and 35 kDa were observed in the RMST pulldown, and not in the controls (beads only and antisenseRMST, ... 8.7) Knockdown of RMST appeared to shut down the neurogenic program of neural stem cells, with the cells adopting a glia lineage instead (Figure 7.6) On the other hand, overexpression of RMST enhanced ... which retained TUJ1 and synaptophysin (SYP) expression typical of neurons (Figure 8.9) In addition, there was no significant change in the expression of a panel of neurogenic and neuronal markers...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
... efficiencies of knockdown are indicated As expected, knockdown of any of the components of the RMST complex resulted in the loss of neurogenesis, indicated by decrease in the number of TUJ1+ and MAP2+ ... Figure 8.15: Co-immunoprecipitation (Co-IP) of hnRNPA2-FLAG and SOX2 in the absence of RNA Cell lysate was treated with RNase, and the co-IP of hnRNPA2 and SOX2 indicated that the two proteins could ... Z., and Ponting, C.P (2010) Long noncoding RNA genes: conservation of sequence and brain expression among diverse amniotes Genome Biol 11, R72 Chu, C., Qu, K., Zhong, F.L., Artandi, S.E., and...
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Clinical significance of cancer stem cell markers and other related proteins in colorectal carcinomas biological and methodological considerations
... Table Comparison of the three protocols for quantity and quality of extracted DNA 49 Level of agreement between human visual and computerassisted machine scoring methods 55 Nature of disagreement ... expression of immunohistochemistry markers 58 Extent of disagreement in reproducibility of results between human visual and computer-assisted scoring methods 58 Frequency of positive expression of protein ... of at least seven proteins including, MLH1 and MSH2 The MLH1 and MSH2 protein are essential in the mismatch repair machinery and mutations in MLH1 and MSH2 have been implicated in HNPCC (Boland...
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Reproductive Senescence in a Long-Lived Seabird: Rates of Decline in Late-Life Performance Are Associated with Varying Costs of Early Reproduction pot
... longstanding general problem in studies of age -related breeding performance and can hamper the detection of senescent declines (Cam and Monnat 2000; Nisbet 2001; Reid et al 2003; van de Pol and ... ! 001) None of the interactions between relative laying date and other terms was significant Effect of Early-Life Output on RLS and LBS Analysis of the relative overall importance of the different ... expense of future survival and future reproduction, and particularly at the expense of somatic maintenance, which would favor increased survival and longevity (Kirkwood 1977; Kirkwood and Rose...
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Tài liệu Evaluation of possible Six Sigma implementation including a DMAIC project pptx
... understanding and by using different data collection methods get a deeper understanding of the studied problem (Holme & Solvang, 1991) Quantitative methods are more formalized and structured and often ... also describes the study of literature, choice of data collection, methodology and chosen tools in the DMAIC project and finally a discussion of the validity and reliability of the thesis Holme & ... a better understanding of the scope of the problem -18- -METHODOLOGYCause -and- Effect Diagram: This tool is helpful to find underlying causes of the problem It's easy to use and often gives appreciable...
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Tài liệu The long-term reproductive health consequences of female genital cutting in rural Gambia: a community-based survey doc
... or II operations and two women who reported being sealed had no signs of closure Of the three main ethnic groups, 98% of Mandinkas, 4% of Wollofs and 32% of Fulas had signs of genital cutting ... Medicine and International Health volume no pp 643±653 august 2001 L Morison et al Long- term consequences of FGC in The Gambia secondary level education (3% of Mandinkas, 6% of Fulas and 1% of Wollofs) ... common and how serious the short- and long- term consequences are is lacking (Obermeyer et al 1999) Hospital -based studies have catalogued types of cutting and morbidity, but give no indication of...
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Tài liệu Handbook of Long-Term Care Administration and Policy pdf
... Art and Practice of Court Administration, Alexander B Aikman 130 Handbook of Globalization, Governance, and Public Administration, edited by Ali Farazmand and Jack Pinkowski 131 Handbook of Globalization ... 91 Handbook of Public Management Practice and Reform, edited by Kuotsai Tom Liou 93 Handbook of Crisis and Emergency Management, edited by Ali Farazmand 94 Handbook of Comparative and Development ... Stuart S Nagel 54 Handbook of Regulation and Administrative Law, edited by David H Rosenbloom and Richard D Schwartz 55 Handbook of Bureaucracy, edited by Ali Farazmand 56 Handbook of Public Sector...
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Tài liệu NEW AND ORIGINAL THEORIES OF THE GREAT PHYSICAL FORCES doc
... theories of the transmission of light and sound; of the production of winds, and sun-spots, and of the method of development and dissemination of heat, are in point of fact, unphilosophical and incomprehensible ... construction of a true philosophy of the physical forces must depend now upon our rightly understanding the modus operandi of the conveyance, and utilization, of these sun-elements, and the workings of ... the direction of other spheres These theories throw new light upon the character and extent of the atmosphere of the moon and planets, and the consequent availability of those and other spheres...
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Tài liệu Báo cáo khoa học: Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt
... mean (symbols) and standard deviation (cross-lines) of four measurements Lines represent best global fit of the data set to the equation of Model I described in the Materials and methods The measure ... parameters of plasmin in the presence of fatty acids The values of kp and Km were determined from the amidolytic assay of plasmin activity, according to Model I, in the presence of oleate (OA) and arachidonate ... Evaluation of kinetic parameters Fig Comparison of three models for the catalytic action of plasmin in the presence of stearate The p-nitroaniline (P) released in the course of hydrolysis of 40 lM...
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Tài liệu Báo cáo khoa học: "TREATMENT OF LONG DISTANCE DEPENDENCIES IN LFG AND TAG: FUNCTIONAL UNCERTAINTY IN LFG IS A COROLLARY IN TAG" ppt
... write alongside each node, the t and b statements, with the t statement written above the b statement Let us say that troo~,broot and tloot= bLoo~ are the t and b statements of the root and foot ... and t are compatible and so are b/oot and b~ If we not adjoin at the node, 7, then we unify t , with b, More details of the definition of FTAG may be found in [8, 9] We now give an example of ... the elementary trees (and hence the grammar) in an extended version of K-K logic (the extension consists of adding Aabstraction and application) 3 LFG AND TAG ANALYSES FOR LONG DISTANCE DEPENDENCIES...
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Infation Accounting and Nonfinancial Corporate Prots: Physical Assets pot
... classes of claimantson the assets and incomeflows of a firm,includingbondholders,banksand othershort-term lenders ,and preferred and common stockholders.In our work, profitsare takento be a measureof ... equityholderswould be equal to the value of the firm'sassets less the value of its liabilities(the value of the claims of the prior claimantson the assets of the firm) The componentof profitsreflectingchange in ... betweentheirsellingpricesand theircosts, and the value of inventoryis the sum of the costs of items in stock Thereis a completecorrespondence between the flow of goods and the reportedflow of costs The accountingprofessionand...
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Ceylon; an Account of the Island Physical, Historical, and Topographical with Notices of Its Natural History, Antiquities and Productions, Volume doc
... quantity of rain in Ceylon and Hindustan (note) Opposite climates of the same mountain Climate of Galle Kandy and its climate Mists and hail Climate of Trincomalie (text and note) Jaffna and its ... Mahawanso Outline of the Mahawanso Turnour's epitome of Singhalese history Historical proofs of the Mahawanso Identity of Sandracottus and Chandragupta Ancient map of Ceylon (note) List of Ceylon sovereigns ... and renown The victory of Dutugaimunu Progress of the south of the island Building of the great Ruanwellé Dagoba Building of the Brazen Palace Its vicissitudes and ruins Death and character of...
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Báo cáo khoa học: Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids docx
... ratio of 2.7 : and observed rates of fatty acid utilization of between 63 and 59 lmolÆh)1 per g dry weight through an initial ÔpulseÕ and subsequent ÔchaseÕ period The extent of activation of the ... 0.03 to Fig Effects of palmitate, insulin and epinephrine on a-2 AMPK activity and malonyl-CoA content Hearts were perfused for 60 with mM glucose and BSA (20 mgÆmL)1) and other additions as indicated ... ratios representative of mild (1 day) and severe (> day) starvation, respectively Over 60 of perfusion we tested the effects of 0.25 and 0.5 mM fatty acids against two control conditions (Table 1)...
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Báo cáo khoa học: "Phrasal Analysis of Long Noun Sequences" pptx
... involved in the recognition of long noun sequences, and in Section we present our proposed solution and describe its implementation Sections and are devoted to related work and to our conclusions, ... Process Model of Nominal Compounding in English American Journal of Computational Linguistics, microfiche 33 1976 Wilensky, R., and Y Arens PHRAN: A Knowledge -Based Natural Language Understander In ... interpretation of long noun sequences There have been several attempts to deal with the problem of producing a meaning representation for a given string of nouns See (Finin, 19~0) and (Reimold,...
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Mechanical properties of solid polymers Constitutive modelling of long and short term behaviour pptx
... study of polymers III Technique of mechanical tests of vulcanizates of rubber and plastics Rubber Chem Tech., 13, 904–917 Haward, R.N (1942) Extension and rupture of cellulose acetate and celluloid ... in the range of temperatures and strain rates of interest A linear dependence of yield stress as a function of log strain rate can be observed over a large interval of strain rates and temperatures, ... prediction thereof is outside the scope of this thesis and requires a more detailed study, including the description of the local stress state The thesis ends in Chapter with some conclusions and recommendations...
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Báo cáo "Numerical study of long wave runup on a conical island " pdf
... conical island setup in a wave basin having the dimension of 30 m wide and 25 m long. The conical island has the shape of a truncated cone with diameters of 7.2 m at the base and ... occurred on the pear‐shaped Okushiri Island, which is approximately 20 km long and 10 km wide (Liu et al., 1995) [7]. In this study, the interaction of long waves and a conical island is investigated ... the line drawing from the center of the island to the point of runup measurement and the y direction. The angle of 0 degree means that the measuring point is at the right side of the island and on the line ...
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