dependent coordinates of material points
Báo cáo y học: "Allele dependent silencing of COL1A2 using small interfering RNAs"
... 82% (Figure 5B), of which 75%, 75% and 73% could be attributed to silencing of the targeted T-allele, respectively Assessment of relative allele abundance of COL1A2 mRNA The software PeakPicker ... used for allele specific silencing irrespective of the location of the actual mutations In this study we have examined the allele dependent effects of seven tiled siRNAs targeting a region surrounding ... allele of rs1800222) 72 hours post-transfection with three different concentrations of siRNA3 Colours of bars indicate the SNP used to calculate allele ratios from cDNA chromatograms in the software...
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Strength of material
... – Materials are selected for specific applications dependent on their properties and characteristics • The behaviour of a material under load is markedly different depending on whether the material ... steel tensile specimen has a diameter of 5mm and a gauge length of 100mm During loading, a sampling pair of data points was taken at 10kN with a new length of 102mm Determine (i) the true stresses ... Tangent modulus: – Slope of the stress-strain curve at a specified level of stress Esec = ∆σ ∆ε ε Unloading Modulus and Plastic Strain • Unloading path is often linear – Slope of an approximate straight...
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Strength of material . lesson 7
... (at speed of sound in material mile s -1 !!) σ σ Kc is material property To measure Kc, (Gc): experimental set up for mode I: (Caution: a is length of edge crack or a is half length of central ... examples of structural failure occurring relating to fatigue Example 1: Stress Concentration P b1 b2 d b3 P A stepped flat bar of mm thick has a hole of 18 mm diameter It has three widths of b1=40 ... with materials getting ‘tired’ due to repeated load cycles The number of cycles is generally quite high For instance the vibration of a aircraft wing during a long flight can result in tens of...
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Tài liệu Báo cáo khoa học: Solution properties of full-length integrin aIIbb3 refined models suggest environment-dependent induction of alternative bent ⁄extended resting states doc
... rotation of the bTD module at the main chain of D606 to dock it against the calf-2 domain with its C-terminus in proximity of the N-terminus of the b3 open TM helix Overall evaluation of the models ... prediction of the O-glycosylation sites (see the Materials and methods), S218 and T615 were selected in place of T259 and T619, respectively, for two O-linked carbohydrates An overview of this model ... swing-out, of $ 6, of the hybrid domain was present in this conformation, consistent with that of $ 10 observed in the avb3 ectodomain tted in the TEM map [7] Finally, after introduction of the mature...
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Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
... core of the particle from lysosomal esterases, or that impaired lipolysis of LDL lipids may block proteolysis of apoB [36] This may arise as a result of the failure of hydrophobic regions of apoB, ... inhibition of the thiol -dependent lysosomal cathepsins by glycated proteins may be of particular importance in apoB turnover The accumulation of glycated apoB within HMDMs may be related to that of the ... experiments The rates of uptake of both the lipid and protein components are not matched by the rate of cellular metabolism of these species, resulting in the accumulation of both unmodified cholesteryl...
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Tài liệu Báo cáo khoa học: Rac upregulates tissue inhibitor of metalloproteinase-1 expression by redox-dependent activation of extracellular signal-regulated kinase signaling pptx
... sustained activation of Rac by overexpression of the Rac-specific activator, Tiam1, or overexpression of V12-Rac1, strongly induces transcriptional upregulation of tissue inhibitor of metalloproteinase ... four different isoforms Of these, the prototype member of the TIMP family, TIMP-1, is of particular interest, because in addition to its classic role as a broad specific inhibitor of active matrix ... well-established roles of (a) AP-1 in regulating the transcription of numerous genes, and (b) of TIMP-1 as an inhibitor of tumor invasion and metastasis as well as a regulator of cell proliferation...
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Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc
... properties of GLD2 are similar to those of the enzymes purified from A niger [1] and A nidulans [2]; that is, the enzyme catalyses the reversible reduction of DHA to glycerol using 4232 Fig SDS ⁄ PAGE of ... 20 gÆL)1 of the main carbon source, as specified, at 28 °C To make mycelial or cell extracts of H jecorina or S cerevisiae, about 100 lg of fresh mycelia or cells were mixed with 300 lL of glass ... Characterization of a glycerol kinase mutant of Aspergillus niger J Gen Microbiol 136, 1299–1305 14 Asako H, Wakita R, Matsumura K, Shimizu M, Sakai J & Itoh N (2005) Purification and cDNA cloning of NADPH-dependent...
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Tài liệu Báo cáo khoa học: Insulin-dependent phosphorylation of DPP IV in liver Evidence for a role of compartmentalized c-Src ppt
... concentration of the soluble form of DPP IV may result in an increased proteolysis of GLP-1 peptides and thus decreased insulin secretion [20] The insulin -dependent phosphorylation of DPP IV observed ... Results are representative of three independent experiments elements by 0.1% Triton X-100 is possible, we were still in a position to address the question of the orientation of c-Src We used the endosomal ... representation of the three major domains (sinusoidal, lateral, bile canalicular) of the hepatocytes present in the PM fraction [50] To the best of our knowledge, tyrosine phosphorylation of DPP IV...
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Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx
... assay of Ras -dependent outgrowth of neurites from PC12 cells [14] These results indicate that even though phosphorylation of Ser916 may contribute to stimulation of the Ras-GEF activity of Ras-GRF1, ... kDa isoform of Ras-GRF1 seems to be expressed at a higher level than the 140 kDa isoform The serotonin-induced increase in phosphorylation of both isoforms was reduced by the coexpression of hPDE4D2 ... mechanism of Ras activation through Gs-coupled receptors is not fully understood In the present study, we show endogenous expression of the Ca2+ -dependent 140 kDa and shorter isoforms of RasGRF1...
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Tài liệu Báo cáo khoa học: Oxygen-dependent regulation of hypoxia-inducible factors by prolyl and asparaginyl hydroxylation pdf
... mechanism of regulation of both domains involves a common iron dependent process [74,75,79] Regulation of HIFa subunits by oxygen -dependent prolyl and asparaginyl hydroxylation A variety of oxygen ... hallmark of von Hippel-Lindau (VHL) disease is the high degree of vascularization, which is due to the Ó FEBS 2003 Mechanisms of oxygen sensing (Eur J Biochem 270) 783 constitutive expression of a ... residues in epidermal growth factor (EGF)-like domains [91] The structures of the catalytic domains of some of these iron dependent hydroxylases have been solved and reveal a conserved double-stranded...
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Báo cáo khoa học: Phosphorylation-dependent binding of human transcription factor MOK2 to lamin A⁄C pot
... [3] One of the clones corresponded to the N-terminal region of JNK-associated leucine zipper (JLP) protein (amino acids 1–141), which is the most recently identified member of the JIP group of scaffold ... Identification of JLP as a partner of hsMOK2 using the yeast two-hybrid screen and identification of the hsMOK2 interaction domain (A) Constructs expressing full-length or the indicated domains of hsMOK2 ... specificity of the interaction between bait and prey was determined by estimating the degree of color development after 90 of incubation in the filter lift b-galactosidase assay, as described in Materials...
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Báo cáo khoa học: Ca2+/calmodulin-dependent kinase II signalling cascade mediates P2X7 receptor-dependent inhibition of neuritogenesis in neuroblastoma cells docx
... of P2X7 receptors in the regulation of neuritogenesis and in the fine control of the balance between proliferation ⁄ differentiation of neuroblastoma cells Results Molecular characterization of ... formation Moreover, we linked the activation of the P2X7 receptor with the regulation of Ca2+ ⁄ calmodulin -dependent kinase II (CaMKII) activity and some of its downstream effectors, including focal ... structures that showed high levels of P2X7-positive immunostaining, suggesting the implication of P2X7 receptors in the control of neuritogenesis (Fig 5G,H) Knockdown of the P2X7 receptor induces neurite...
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Báo cáo khoa học: Context-dependent effects of proline residues on the stability and folding pathway of ubiquitin docx
... structural analysis of F45W mutants of the yeast protein confirms this We have explored the context -dependent effects of proline on ubiquitin stability by introducing a number of substitutions at ... van’t Hoff analysis are close to %5000 JÆK)1Æmol)1 [58,59] It is not entirely clear whether burial of 66–71% of the hydrophobic surface area of the native state should account for all of the ... capacity and entropy of formation of the TSE for folding (U-à) Reported errors reflect the quality of the nonlinear least squares fit to the experimental data We have used the F45W mutant of yeast ubiquitin...
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Đề tài " Diophantine approximation on planar curves and the distribution of rational points " pptx
... systems close to a curve in Rn Proof of Theorem 4.1 The ubiquity version of Theorem 4.2 An auxiluary lemma 4.3 Proof of Theorem Proof of Theorem Proof of Theorem Proof of Theorem Various generalizations ... APPROXIMATION ON PLANAR CURVES 2.3 Proof of Theorem To a certain degree the proof follows the same line of argument as the proof of the convergent part of Theorem In par∗ ticular, it suffices to ... completes the proof of Theorem 10 in the case that G is finite C.3 Proof of Theorem 10: G = ∞ The proof of Theorem 10 in the case that G is infinite follows the same strategy as the proof when G is...
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Báo cáo khoa học: Photosynthetic acclimation: Structural reorganisation of light harvesting antenna – role of redox-dependent phosphorylation of major and minor chlorophyll a/b binding proteins pot
... Mechanism of state transitions General mechanism The process of state transitions represents a short-term adaptation of the photosynthetic apparatus to the conditions of imbalanced illumination of PSII ... screening assays for identification of mutants affected in the signalling cascade of this process These types of screening have led to isolation of the series of stm [58] and stt [59] mutants deficient ... destabilization of the LHCII–PSII supercomplex upon dissociation of hyperphosphorylated CP29, triggering a large increase of the PSI antenna absorption crosssection as a result of the availability of an...
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Báo cáo khoa học: Tumor suppressor p16INK4a ) modulator of glycomic profile and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells ppt
... direction of expression levels of these two a2,3-sialyltransferase genes for enzymes of similar substrate specificity for N-glycans may act in a compensatory manner, the reduction of gene expression of ... New function of p16INK4a ´ S Andre et al Fig Effect of sialidase treatment on the cell-surface binding of plant lectins Semilogarithmic representation of fluorescent surface staining of mock-transfected ... ⁄ nuclear run-off experiments, by proteomic profiling and by flow cytofluorometry Identification of up-regulation of galectin-1 expression Quantitative determination of the presence of galectin1-specific...
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Báo cáo khoa học: Atrial natriuretic peptide-dependent photolabeling of a regulatory ATP-binding site on the natriuretic peptide receptor-A pptx
... ANP -dependent photolabeling of DGC The inset indicates the curve slope and the 50% inhibitory concentration (IC50) of ATP Each data point represents the average arbitrary units of photolabeling of ... effect of ATP on NPR-A Our results, showing co-operativity of ATP inhibition of 8-azido-ATP-B photolabeling, characterized by a Hill coefficient of 2.2, suggest a mechanism by which binding of one ... as the percentage binding of specific 125I-labeled ANP and represents the mean ± SEM of duplicates This figure is representative of three identical experiments 5575 ANP -dependent ATP binding on...
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Báo cáo khoa học: "Learning Correlations between Linguistic Indicators and Semantic Constraints: Reuse of Context-Dependent Descriptions of Entities" doc
... call the set of all descriptions related to the same entity in a corpus, a profile of that entity Profiles for a large number of entities were compiled using our earlier system, PROFILE (Radev ... variety in the size of the profile (number of distinct descriptions) for different entities Table shows a subset of the profile for Ung Huot, the former foreign minister of Cambodia, who was ... covers a variety of pre-modifier and appositional noun phrases For each word wi in a description, we use a version of WordNet to extract the synset offset of the immediate parent of wi 4.2 Finding...
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Báo cáo khoa học: Endogenous mono-ADP-ribosylation mediates smooth muscle cell proliferation and migration via protein kinase N-dependent induction of c-fos expression potx
... actions of MIBG result from its ability to prevent Rho -dependent activation of SRF Discussion The results of this study indicate that an arginine -dependent mART is activated upon stimulation of SMC ... are representative of three independent experiments, each of which exhibited similar results labeling of the 140, 133, 116, 95, 82 and 73 kDa proteins, while the labeling of several proteins ... (MIBG), a decoy substrate of arginine -dependent mono-ADP-ribosylation that consequently reduces modification of endogenous protein targets [29], for 10 prior to addition of various growth stimulating...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25