density dependent transition of polyelectrolyte layers

Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx

Ngày tải lên : 19/02/2014, 02:20

... core of the particle from lysosomal esterases, or that impaired lipolysis of LDL lipids may block proteolysis of apoB [36] This may arise as a result of the failure of hydrophobic regions of apoB, ... inhibition of the thiol -dependent lysosomal cathepsins by glycated proteins may be of particular importance in apoB turnover The accumulation of glycated apoB within HMDMs may be related to that of the ... experiments The rates of uptake of both the lipid and protein components are not matched by the rate of cellular metabolism of these species, resulting in the accumulation of both unmodiﬁed cholesteryl...

12
604
0

Mechanisms for delayed density-dependent reproductive traits in ﬁeld voles, Microtus agrestis: the importance of inherited environmental effects doc

Ngày tải lên : 05/03/2014, 17:20

... inverse of the estimated probability of survival in the estimation (see Littell et al 1996) Hence, the sum of weightings of animals of a particular characteristic divided by the total sum of weightings ... Capture body mass (g) % survived of total Ratio max/min weights −1.76 [−3.42, −0.30] −0.08 [−0.22, 0.03] 60% of 85 24% of 91 1.0 3.7 +0.21 [0.08, 0.39] 53% of 28 42% of 50 1.0 6.4 0.14 [−0.60,0.90] ... beginning of the year, there were differences in density between our two study areas, so the differences in maturation rate may partly have been due to direct density- dependent social suppression of...

13
502
0

Indication of density dependent changes in growth and maturity of the barndoor skate on georges bank

Ngày tải lên : 04/09/2015, 12:38

... 10.1080/19425120.2013.824941 ARTICLE Indication of Density- Dependent Changes in Growth and Maturity of the Barndoor Skate on Georges Bank Karson Coutr´ * e Marine Science Center, University of New England, 11 Hills ... month of capture 14 12 10 -2 10 12 14 16 Number of bands (age) of reader one FIGURE Age-bias plot (grey line) for pairwise comparison of 244 Barndoor Skate vertebral counts made by two independent ... to suggest observable density- dependent changes in the life history characteristics of a batoid species When the VBGFs of Gedamke et al (2005) were compared with those of the current study a...

11
448
0

Báo cáo y học: "Allele dependent silencing of COL1A2 using small interfering RNAs"

Ngày tải lên : 03/11/2012, 11:52

... 82% (Figure 5B), of which 75%, 75% and 73% could be attributed to silencing of the targeted T-allele, respectively Assessment of relative allele abundance of COL1A2 mRNA The software PeakPicker ... used for allele specific silencing irrespective of the location of the actual mutations In this study we have examined the allele dependent effects of seven tiled siRNAs targeting a region surrounding ... allele of rs1800222) 72 hours post-transfection with three different concentrations of siRNA3 Colours of bars indicate the SNP used to calculate allele ratios from cDNA chromatograms in the software...

5
465
0

Tài liệu Báo cáo khoa học: Solution properties of full-length integrin aIIbb3 reﬁned models suggest environment-dependent induction of alternative bent ⁄extended resting states doc

Ngày tải lên : 18/02/2014, 04:20

... rotation of the bTD module at the main chain of D606 to dock it against the calf-2 domain with its C-terminus in proximity of the N-terminus of the b3 open TM helix Overall evaluation of the models ... swing-out, of $ 6, of the hybrid domain was present in this conformation, consistent with that of $ 10 observed in the avb3 ectodomain tted in the TEM map [7] Finally, after introduction of the mature ... already extended, with the transition to the open form (involving the swing-out of the b3 hybrid domain [6]) taking place without the requirement of full separation of the TM helices Since then,...

13
522
0

Tài liệu Báo cáo khoa học: Rac upregulates tissue inhibitor of metalloproteinase-1 expression by redox-dependent activation of extracellular signal-regulated kinase signaling pptx

Ngày tải lên : 19/02/2014, 05:20

... sustained activation of Rac by overexpression of the Rac-speciﬁc activator, Tiam1, or overexpression of V12-Rac1, strongly induces transcriptional upregulation of tissue inhibitor of metalloproteinase ... four different isoforms Of these, the prototype member of the TIMP family, TIMP-1, is of particular interest, because in addition to its classic role as a broad speciﬁc inhibitor of active matrix ... well-established roles of (a) AP-1 in regulating the transcription of numerous genes, and (b) of TIMP-1 as an inhibitor of tumor invasion and metastasis as well as a regulator of cell proliferation...

16
485
0

Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc

Ngày tải lên : 19/02/2014, 06:20

... properties of GLD2 are similar to those of the enzymes purified from A niger [1] and A nidulans [2]; that is, the enzyme catalyses the reversible reduction of DHA to glycerol using 4232 Fig SDS ⁄ PAGE of ... 20 gÆL)1 of the main carbon source, as specified, at 28 °C To make mycelial or cell extracts of H jecorina or S cerevisiae, about 100 lg of fresh mycelia or cells were mixed with 300 lL of glass ... Characterization of a glycerol kinase mutant of Aspergillus niger J Gen Microbiol 136, 1299–1305 14 Asako H, Wakita R, Matsumura K, Shimizu M, Sakai J & Itoh N (2005) Purification and cDNA cloning of NADPH-dependent...

7
510
0

Tài liệu Báo cáo khoa học: Insulin-dependent phosphorylation of DPP IV in liver Evidence for a role of compartmentalized c-Src ppt

Ngày tải lên : 19/02/2014, 07:20

... concentration of the soluble form of DPP IV may result in an increased proteolysis of GLP-1 peptides and thus decreased insulin secretion [20] The insulin -dependent phosphorylation of DPP IV observed ... Results are representative of three independent experiments elements by 0.1% Triton X-100 is possible, we were still in a position to address the question of the orientation of c-Src We used the endosomal ... representation of the three major domains (sinusoidal, lateral, bile canalicular) of the hepatocytes present in the PM fraction [50] To the best of our knowledge, tyrosine phosphorylation of DPP IV...

12
738
0

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

Ngày tải lên : 19/02/2014, 18:20

... assay of Ras -dependent outgrowth of neurites from PC12 cells [14] These results indicate that even though phosphorylation of Ser916 may contribute to stimulation of the Ras-GEF activity of Ras-GRF1, ... kDa isoform of Ras-GRF1 seems to be expressed at a higher level than the 140 kDa isoform The serotonin-induced increase in phosphorylation of both isoforms was reduced by the coexpression of hPDE4D2 ... mechanism of Ras activation through Gs-coupled receptors is not fully understood In the present study, we show endogenous expression of the Ca2+ -dependent 140 kDa and shorter isoforms of RasGRF1...

13
730
0

Tài liệu Báo cáo khoa học: Oxygen-dependent regulation of hypoxia-inducible factors by prolyl and asparaginyl hydroxylation pdf

Ngày tải lên : 20/02/2014, 23:20

... mechanism of regulation of both domains involves a common iron dependent process [74,75,79] Regulation of HIFa subunits by oxygen -dependent prolyl and asparaginyl hydroxylation A variety of oxygen ... hallmark of von Hippel-Lindau (VHL) disease is the high degree of vascularization, which is due to the Ó FEBS 2003 Mechanisms of oxygen sensing (Eur J Biochem 270) 783 constitutive expression of a ... residues in epidermal growth factor (EGF)-like domains [91] The structures of the catalytic domains of some of these iron dependent hydroxylases have been solved and reveal a conserved double-stranded...

10
603
0

Báo cáo khoa học: Phosphorylation-dependent binding of human transcription factor MOK2 to lamin A⁄C pot

Ngày tải lên : 07/03/2014, 01:20

... [3] One of the clones corresponded to the N-terminal region of JNK-associated leucine zipper (JLP) protein (amino acids 1–141), which is the most recently identified member of the JIP group of scaffold ... Identification of JLP as a partner of hsMOK2 using the yeast two-hybrid screen and identification of the hsMOK2 interaction domain (A) Constructs expressing full-length or the indicated domains of hsMOK2 ... Harper et al Phosphorylation -dependent binding of MOK2 A D B E C Fig The N-terminal domains of JLP and JSAP1 bind to hsMOK2 in vitro (A) Mapping the interaction region of JLP using GST pull-down...

11
378
0

Báo cáo khoa học: Ca2+/calmodulin-dependent kinase II signalling cascade mediates P2X7 receptor-dependent inhibition of neuritogenesis in neuroblastoma cells docx

Ngày tải lên : 07/03/2014, 02:20

... of P2X7 receptors in the regulation of neuritogenesis and in the ﬁne control of the balance between proliferation ⁄ differentiation of neuroblastoma cells Results Molecular characterization of ... formation Moreover, we linked the activation of the P2X7 receptor with the regulation of Ca2+ ⁄ calmodulin -dependent kinase II (CaMKII) activity and some of its downstream effectors, including focal ... evoked by BzATP were of 162.8 ± 53.19 pA (n = 25 cells) which, considering an average membrane capacitance of 11.62 ± 1.09 pF (n = 27 cells), implies a mean current density of about 14 pAÆpF)1...

19
328
0

Báo cáo khoa học: Context-dependent effects of proline residues on the stability and folding pathway of ubiquitin docx

Ngày tải lên : 07/03/2014, 16:20

... buffered solution of native protein was unfolded by a : 10 dilution to yield ﬁnal concentrations of GdmCl near or above the midpoint of the equilibrium unfolding transition (concentrations of GdmCl in ... structural analysis of F45W mutants of the yeast protein conﬁrms this We have explored the context -dependent effects of proline on ubiquitin stability by introducing a number of substitutions at ... van’t Hoff analysis are close to %5000 JÆK)1Æmol)1 [58,59] It is not entirely clear whether burial of 66–71% of the hydrophobic surface area of the native state should account for all of the...

11
549
0

Báo cáo khoa học: Photosynthetic acclimation: Structural reorganisation of light harvesting antenna – role of redox-dependent phosphorylation of major and minor chlorophyll a/b binding proteins pot

Ngày tải lên : 16/03/2014, 06:20

... Mechanism of state transitions General mechanism The process of state transitions represents a short-term adaptation of the photosynthetic apparatus to the conditions of imbalanced illumination of PSII ... and J Barber Structure of photosystems in state transitions Fig Diagrammatic overview of the state transitions process Under conditions of balanced light illumination of photosystems, a linear ... identiﬁcation of mutants affected in the signalling cascade of this process These types of screening have led to isolation of the series of stm [58] and stt [59] mutants deﬁcient in state transitions...

13
343
0

Báo cáo khoa học: Tumor suppressor p16INK4a ) modulator of glycomic proﬁle and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells ppt

Ngày tải lên : 16/03/2014, 10:20

... direction of expression levels of these two a2,3-sialyltransferase genes for enzymes of similar substrate specificity for N-glycans may act in a compensatory manner, the reduction of gene expression of ... New function of p16INK4a ´ S Andre et al Fig Effect of sialidase treatment on the cell-surface binding of plant lectins Semilogarithmic representation of fluorescent surface staining of mock-transfected ... ⁄ nuclear run-off experiments, by proteomic profiling and by flow cytofluorometry Identification of up-regulation of galectin-1 expression Quantitative determination of the presence of galectin1-specific...

24
395
0

Báo cáo khoa học: Atrial natriuretic peptide-dependent photolabeling of a regulatory ATP-binding site on the natriuretic peptide receptor-A pptx

Ngày tải lên : 16/03/2014, 23:20

... ANP -dependent photolabeling of DGC The inset indicates the curve slope and the 50% inhibitory concentration (IC50) of ATP Each data point represents the average arbitrary units of photolabeling of ... effect of ATP on NPR-A Our results, showing co-operativity of ATP inhibition of 8-azido-ATP-B photolabeling, characterized by a Hill coefficient of 2.2, suggest a mechanism by which binding of one ... as the percentage binding of specific 125I-labeled ANP and represents the mean ± SEM of duplicates This figure is representative of three identical experiments 5575 ANP -dependent ATP binding on...

12
338
0

Báo cáo khoa học: "Learning Correlations between Linguistic Indicators and Semantic Constraints: Reuse of Context-Dependent Descriptions of Entities" doc

Ngày tải lên : 17/03/2014, 07:20

... call the set of all descriptions related to the same entity in a corpus, a profile of that entity Profiles for a large number of entities were compiled using our earlier system, PROFILE (Radev ... variety in the size of the profile (number of distinct descriptions) for different entities Table shows a subset of the profile for Ung Huot, the former foreign minister of Cambodia, who was ... covers a variety of pre-modifier and appositional noun phrases For each word wi in a description, we use a version of WordNet to extract the synset offset of the immediate parent of wi 4.2 Finding...

7
325
0

Báo cáo khoa học: Endogenous mono-ADP-ribosylation mediates smooth muscle cell proliferation and migration via protein kinase N-dependent induction of c-fos expression potx

Ngày tải lên : 17/03/2014, 09:20

... actions of MIBG result from its ability to prevent Rho -dependent activation of SRF Discussion The results of this study indicate that an arginine -dependent mART is activated upon stimulation of SMC ... are representative of three independent experiments, each of which exhibited similar results labeling of the 140, 133, 116, 95, 82 and 73 kDa proteins, while the labeling of several proteins ... (MIBG), a decoy substrate of arginine -dependent mono-ADP-ribosylation that consequently reduces modiﬁcation of endogenous protein targets [29], for 10 prior to addition of various growth stimulating...

10
389
0

Báo cáo Y học: Autophosphorylation-dependent inactivation of plant chimeric calcium/calmodulin-dependent protein kinase docx

Ngày tải lên : 17/03/2014, 17:20

... activity of CCaMK Autophosphorylation of CCaMK was associated with a time dependent loss of the Ca2+/CaM -dependent enzyme activity at pH 6.5, 7.5 and 8.5 (Fig 1) Activity measurements of enzymes ... phosphorylation of threonine 267 [12] In this study, we report autophosphorylation -dependent inactivation of CCaMK Autophosphorylation of CCaMK resulted in time -dependent loss of enzyme activity ... Mechanism of autophosphorylation of the multifunctional Ca2+/calmodulin -dependent protein kinase J Biol Chem 260, 6427–6433 17 Yamauchi, T & Fujisawa, H (1985) Self-regulation of calmodulin-dependent...

7
251
0

Báo cáo khoa học: Lipid-induced conformational transition of the amyloid core fragment Ab(28–35) and its A30G and A30I mutants docx

Ngày tải lên : 23/03/2014, 07:20

... Interactions of Ab(28–35) and its mutants with lipids S Nagarajan et al One of the neuropathological features in the brain of patients with Alzheimer’s disease is the presence of extracellular ... CD spectra of the WT and A30I peptides showed a b-sheet structure in the presence of PG vesicles For the A30G mutant, a reduction of the coil peak at 197 nm and a concomitant increase of the helix ... exposure of the vesicles to the peptides [see the 1,6-diphenyl-1,3,5-hexatriene (DPH) studies below] Conﬁrming the involvement of electrostatic charges in the conformational transitions of the...

13
336
0