dendritic cell subsets and immune regulation
Báo cáo y học: "Enumeration and phenotypical analysis of distinct dendritic cell subsets in psoriatic arthritis and rheumatoid arthritis" pps
... Jongbloed et al Figure Enumeration of dendritic cell subsets in various arthritides and healthy volunteers Plasmacytoid dendritic cells (pDCs) and myeloid dendritic cells volunteers (mDCs) are significantly ... of blood derived DC subsets Rheumatoid arthritis (RA) and psoriatic arthritis (PsA) peripheral blood (PB) plasmacytoid DC subsets dendritic cells (pDCs) and myeloid dendritic cells (mDCs) have ... mDCs and pDCs from RA PB and PsA PB, we also evaluated expression on SF-derived cell subsets CD62L was decreased on mDCs and pDCs from both RA SF and PsA SF DC subsets compared with RA PB and...
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Báo cáo y học: "Dendritic cell subsets dynamics and cytokine production in SIVmac239-infected Chinese rhesus macaques" pptx
... Palucka K: Immunobiology of dendritic cells Annu Rev Immunol 2000, 18:767-811 Liu YJ: Dendritic cell subsets and lineages, and their functions in innate and adaptive immunity Cell 2001, 106:259-262 ... Thomson AW: Dendritic cell subsets in blood and lymphoid tissue of rhesus monkeys and their mobilization with Flt3 ligand Blood 2003, 102:2513-2521 Banchereau J, Steinman RM: Dendritic cells and the ... correlations between CD4+ T -cell counts and DC subset counts or cytokine production (A) CD4+ T -cell counts and mDC counts; (B) CD4+ T -cell counts and pDC counts; (C) CD4+ T -cell counts and IL-12 concentration;...
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Báo cáo y học: "Phenotype changes and impaired function of dendritic cell subsets in patients with sepsis: a prospective observational analysi" ppsx
... Circulating monocyte and dendritic cell subset frequencies in sepsis patients Frequencies of circulating monocyte and dendritic cell subsets in Circulating monocyte and dendritic cell subset frequencies ... circulating monocyte and dendritic cell subsets in patients with sepsis at baseline and at day 28 compared with healthy controls Monocyte and dendritic cell subsets were stained and gated as described ... using a standardized assay as described in Materials and methods, and is given as HLA-DR antibodies per cell (Ab /cell) (b) For plasmacytoid dendritic cells (PDCs) and myeloid dendritic cells (MDCs),...
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Báo cáo y học: "Novel insights into the relationships between dendritic cell subsets in human and mouse revealed by genome-wide expression profiling" ppt
... levels in LN-DC subsets and CD16 cells than in monocytes, neutrophils and lymphocytes, attesting to the existence of biological functions common, and specific, to DC subsets and CD16 cells Thus, ... cDC subsets, B cells and T cells [11] or on cDCs and pDCs [15] Most of the data for the mouse 430 2.0 compendium were generated in-house, with the exceptions being CD4 T cells and myeloid cells ... vivo generation of human dendritic cell subsets by Flt3 ligand Blood 2000, 96:878-884 Bonasio R, von Andrian UH: Generation, migration and function of circulating dendritic cells Curr Opin Immunol...
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Báo cáo sinh học: "Cancer stem cell subsets and their relationship" pot
... Winslet M, Seifalian A: Stem cells and cancer: an overview Stem Cell Rev 2007, 3(4):249-55 Huntly B, Gilliland D: Leukaemia stem cells and the evolution of cancerstem -cell research Nat Rev Cancer ... precancerous stem cells Chen et al [61] reported the isolation of a type of precancerous stem cells from dendritic cell- like leukemic mice and the establishment of this precancerous stem cell line The ... stem cells, and neoplastic ductal is not precanerous lesion but cancer foci [67] Second is the genotype and phenotype Primary cancer stem cells are derived from precancerous stem cells and exhibit...
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báo cáo hóa học:" Cancer stem cell subsets and their relationships" pot
... Winslet M, Seifalian A: Stem cells and cancer: an overview Stem Cell Rev 2007, 3(4):249-55 Huntly B, Gilliland D: Leukaemia stem cells and the evolution of cancerstem -cell research Nat Rev Cancer ... precancerous stem cells Chen et al [61] reported the isolation of a type of precancerous stem cells from dendritic cell- like leukemic mice and the establishment of this precancerous stem cell line The ... stem cells, and neoplastic ductal is not precanerous lesion but cancer foci [67] Second is the genotype and phenotype Primary cancer stem cells are derived from precancerous stem cells and exhibit...
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Báo cáo y học: "CTLA-4 blockade during dendritic cell based booster vaccination influences dendritic cell survival and CTL expansion" ppt
... Banchereau J, Steinman RM: Dendritic cells and the control of immunity Nature 1998, 392:245-52 Gad M, Claesson MH, Pedersen AE: Dendritic cells in peripheral tolerance and immunity APMIS 2003, ... Amigorena S: Antigen presentation and T cell stimulation by dendritic cells Annu Rev Immunol 2002, 20:621-67 Figdor CG, DE Vries IJ, Lesterhuis WJ, Melief CJ: Dendritic cell immunotherapy:Mapping the ... Journal of Immune Based Therapies and Vaccines 2007, 5:9 where adjusted%survival = 100 × (%survival of Ag+ cells/(average % survival of Ag+ cells in naïve mice in the absence of effector cells))...
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Báo cáo sinh học: " Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice" docx
... detaching, CD4+ T cells were stained with CD25 MicroBeads (Miltenyi Biotec) and CD4+CD25+T cells were positively and negatively selected Separation was controlled by FCM and Spleen CD4+ T cells were ... sensitized and challenged with OVA, OVA- pcDNA3.1-vaccinated mice sensitized and challenged with OVA, and OVA-Fc-pcDNA3.1-vaccinated mice sensitized and challenged with OVA * and ** stand for the ... sensitized and challenged with OVA, OVA- pcDNA3.1-vaccinated mice sensitized and challenged with OVA, and OVA-Fc-pcDNA3.1-vaccinated mice sensitized and challenged with OVA * and ** stand for the...
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Role of p73 in regulation of cell death specific role in mitotic cell death and potential regulation of caspase 2s
... 2.2.3.1 Cell death assays 2.2.3.2 Cell cycle and phospho-histone-H3 analysis 2.2.4 Immunofluorescence and confocal microscopy 2.2.5 Protein isolation and analysis 2.2.5.1 Total cell lysate 2.2.5.2 Cell ... of Bubr1 and Mad2 after knockdown of p73 105 Fig 3.28: Different mechanism of action between p73 and Bubr1 and Mad2 in regulation of mitotic cell death 107 Fig 3.29: Knockdown of Bubr1 and Mad2 ... the other hand, depends on the engagement of death receptors on the cell surface Immune cells activate this extrinsic pathway during tumor surveillance to kill tumor cells Binding of ligands such...
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Báo cáo y học: " Chitosan Interferon-c Nanogene Therapy for Lung Disease: Modulation of T-Cell and Dendritic Cell Immune Responses" pps
... collagenase and DNase, and passed through a cell strainer to produce single -cell suspensions The cells were pelleted by centrifugation at 500g for minutes at 4uC, and then red blood cells were ... nodes through 40-micron cell strainers, and CD11c+ cells were isolated by magnetic bead separation The cells were stained for CD11b and separately for each of the dendritic cell activation markers ... 15,000 cells and were substantiated by a repeat experiment cells and either the same or slightly more Th1 cells than control mice (Figure 1A) Both CD4+ and CD8+ T lymphocytes are involved in the immune...
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Báo cáo y học: "Emerging mechanisms of immune regulation: the extended B7 family and regulatory T cell" pdf
... antibody isotype switching and germinal center formation CD40 and CD40 ligand (CD40L) could be important in stabilizing the ICOS–B7h interaction between T cells and naive B cells and in promoting germinal ... there is differential regulation of Th1 and Th2 cells by these ligands B7-H3 and B7x: last-ditch regulators of the peripheral tissues? B7-H3 and B7x (also called B7-H4 and B7-S1) are the most ... tumor immune evasion and would also be a suitable target of anti-tumor immunotherapy Costimulation and various regulatory T cells: FOXP3, GITR, and ‘anti-suppression’ To understand how the T cell...
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Innate Immune Regulation and Cancer Immunotherapy pot
... the concept of immune regulation and cell- mediated immune suppression Chapters and provide overviews of our current understanding of human and mouse dendritic cell (DC) subsets and their regulatory ... the host immune system and cancer cells Innate immunity is the first line of host defense against pathogens and transformed tumor cells Innate immune cells including NK, NKT, and dgT cells have ... cytokine milieu and the presence or absence of other immune cells (Greten et al 2004; Karin and Greten 2005) For example, IL-1, IL-6, IL-8, and TGF-b released by immune cells and tumor cells promote...
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Báo cáo hóa học: " Effects of pegylated G-CSF on immune cell number and function in patients with gynecological malignancies" doc
... × 103 cells/μl, range 0.8-2.24; and 1.21 × 103 cells/μl, range 0.45-2.54, in the filgrastim and pegfilgrastim group, respectively; monocyte count = 0.25 × 10 cells/μl, range 0.05-0.35; and 0.23 ... circulation However, the relative distribution of CD4 + T cells, CD8 + T cells, CD19 + B cells and NK cells (defined as CD3 - CD16 + CD56 + cells) within the lymphocyte population was unaffected ... increases CD123hi blood dendritic cells with altered CD62L and CCR7 expression Blood 2003, 101:2314-2317 Trinchieri G: Interleukin-12 and the regulation of innate resistance and adaptive immunity...
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Báo cáo sinh học: "Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets" doc
... miR-20a and miR-92 were therefore determined by single specific qPCR in differentiated CD8+ T cell subsets, and compared to the levels found in naïve cells In agreement with the TLDA data, and despite ... CD45RA- cells can be further divided in two functionally distinct subsets, with CD28 + cells (early effector memory (E-EM) closer to central memory cells) being less differentiated than CD28 - subsets ... differentiated CD8+ T cells when compared to naive cells The microRNA expression regulation uncovered here is likely to strongly influence the gene signature of these different subsets, and therefore...
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Báo cáo sinh học: "Concomitant detection of IFNa signature and activated monocyte/dendritic cell precursors in the peripheral blood of IFNa-treated subjects at early times after repeated local cytokine treatments" doc
... IFNa-induced chemokine, which binds and activates the seven transmenbrane G-protein-coupled receptor CXCR3, and is expressed especially in activated Th1 cells, B cells, NK cells and DC, thus suggesting that ... Immune response induced in vitro by CD16- and CD16+ monocytederived dendritic cells in patients with metastatic renal cell carcinoma treated with dendritic cell vaccines J Clin Immunol 2004, 24:86-96 ... and anti-CD86 (Pharmingen) Samples were collected and analyzed by using a FACSCalibur (Becton Dickinson) and data analysis was performed by FlowJo software (Tree-Star), excluding dead cells and...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... Figure Morphology, phenotype, and function of CD11b+ and CD33+ MDSC subsets A, Morphology of human CD33+ and CD11b+ MDSC subsets isolated after tumor cell line co-culture and normal myeloid counterparts ... cell line-induced CD33+ and breast cancer cell line-induced CD11b+ MDSC compared with medium only, non-suppressive CD33+ and CD11b+ cells as measured by flow cytometry Mean percent positive cells ... small cell lung for CD11b+) showed an abundance of immature cells, including metamyelocytes or band cells and blast-like cells (middle left and far right panels, representative images shown of three...
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báo cáo hóa học:" Effect of borax on immune cell proliferation and sister chromatid exchange in human chromosomes Malinee Pongsavee" docx
... material and immune cell development involves in human carcinogenesis In this study, we studied about the toxic effects of borax on immune cell proliferation (lymphocyte proliferation) and genotoxicity ... chromosome-banding patterns In human chromosomes, SCEs occur preferentially in Q-negative bands or at the junction of Q-positive and Q-negative regions [10] The toxic effects of borax to immune cell ... results indicated the correlation between the immune cell proliferation and the borax cytotoxicity Results Studying the effect of borax on immune cell proliferation (lymphocyte proliferation)...
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báo cáo hóa học:" Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets" pptx
... miR-20a and miR-92 were therefore determined by single specific qPCR in differentiated CD8+ T cell subsets, and compared to the levels found in naïve cells In agreement with the TLDA data, and despite ... CD45RA- cells can be further divided in two functionally distinct subsets, with CD28 + cells (early effector memory (E-EM) closer to central memory cells) being less differentiated than CD28 - subsets ... differentiated CD8+ T cells when compared to naive cells The microRNA expression regulation uncovered here is likely to strongly influence the gene signature of these different subsets, and therefore...
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