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deletion screen for lethal or sterile recessive mutations

Báo cáo y học:

Báo cáo y học: "A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells" doc

Báo cáo khoa học

... genome-wide negative selection screens for genes that confer resistance to infection Results Screening strategy for infection resistant mutants The overall strategy for the screen is illustrated in ... which offers a major advantage in recessive screens Indeed, two reports have described successful use of Blm-deficient ES cells to identify recessive mutations in genes required for DNA mismatch ... involve more than one molecule; for instance, HIV requires a co-receptor in addition to CD4 [4] mCat-1, which encodes a cationic amino acid transporter, was identified as the receptor for murine...
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Tài liệu We Cannot Trade Quality for Schedule or Budget! ppt

Tài liệu We Cannot Trade Quality for Schedule or Budget! ppt

Quản trị mạng

... We Cannot Trade Quality for Schedule or Budget! Alan S Koch, Global Knowledge Instructor, PMP Abstract It is not uncommon for people to say, “Fast, cheap, or good—choose two.” Most people ... IT Project Management Triple Constraints of Project Management For more information or to register, visit www.globalknowledge.com or call 1-866-925-7765 to speak with a sales representative Our ... Process (TSP) Mr Koch: • Authored Agile Software Development: Evaluating the Methods for your Organization, Artech House • Authored "TSP can be the Building Blocks for CMMI", Crosstalk Magazine,...
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Tài liệu Project Management Practices: The Criteria for Success or Failure docx

Tài liệu Project Management Practices: The Criteria for Success or Failure docx

Quản lý dự án

... when more information becomes available as a project progresses Also plans are not used correctly or used to guide the project forward, thus causing the project to fail 5.2 Implementation factor ... this work should be copied or reproduced in digital, hard, or any other format for commercial use without written permission To purchase reprints of this article please email: admin@ibima.org References ... The Criteria for Success or Failure Causes of project failure Projects fail mainly because of unable to plan and estimate correctly, or fail to implement the tasks according to plan or failure...
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Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx

Tài liệu Báo cáo khoa học: Complex transcriptional and translational regulation of iPLA2c resulting in multiple gene products containing dual competing sites for mitochondrial or peroxisomal localization docx

Báo cáo khoa học

... had the potential for providing substrate for mitochondrial fatty acid oxidation by lipid hydrolysis [7], for generating lipid second messengers (eicosanoids and lysolipids), for modulating ion ... imported proteins and their targeting sequences Eur J Biochem 241, 779–786 Luckow, V.A & Summers, M.D (1988) Signals important for high-level expression of foreign genes in Autographa californica ... method for viral plaque titering were performed utilizing the Bac-to-Bac Baculovirus Expression System (Invitrogen) according to the manufacturer’s instructions Sf9 cells were grown and infected for...
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Báo cáo khóa học: Modified colorimetric assay for uricase activity and a screen for mutant Bacillus subtilis uricase genes following StEP mutagenesis pptx

Báo cáo khóa học: Modified colorimetric assay for uricase activity and a screen for mutant Bacillus subtilis uricase genes following StEP mutagenesis pptx

Báo cáo khoa học

... (IPTG) was spread on LB pates for screening Screening for uricase-producing microorganisms The scheme used for screening for uricase-producing microorganisms, or in this case, E coli carrying an active ... EcoRI/HindIII predigested pMAL vector using a DNA ligation Kit to transform E coli DH5a selecting for ampicillin resistance Isopropyl thio-b-D-galactoside (IPTG) was spread on LB pates for screening ... sequences were performed at the Fig Flow chart for the detection of uricase activity by the conventional method and the modified colorimetric method National Center for Biotechnology Information (NCBI)...
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for sex or money

for sex or money

Tiếng anh

... these hoops none of us would have been born The point is that you cannot regulate manners Even the victims agree that these type of strategies will not work Sexual harassment problems are power ... and your job Sexual harassment is your boss coming on to you more than once It is not little kids on a playground exchanging pecks Nor is it a kiss good-night after a first date Sexual harassment ... will not work Sexual harassment problems are power problems, not manner problems If the administrators want to curb problems then they should set up harsher penalties, not harsher preventive measures...
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báo cáo hóa học:

báo cáo hóa học: " The Psychosocial Screen for Cancer (PSSCAN): Further validation and normative data" pptx

Hóa học - Dầu khí

... psychological treatment only if it is for patients with a diagnosed disorder No information on cut-off scores and their meaning for diagnosis was available for PSSCAN when it was first published ... brackets) Anxiety Subclinical Disorder (= or >) AUC = 0.85 Clinical Disorder (= 11 or >) AUC = 0.99 Depression Subclinical Disorder (= or >) AUC = 0.88 Clinical Disorder (= 11 or >) AUC = 0.91 Sensitivity ... diabetes, or an autoimmune disease, or any other disease of similar severity (participants provided this information in an open response form) A minimum age threshold of 40 years of age was set for...
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báo cáo hóa học:

báo cáo hóa học: " Clinical usefulness of the screen for cognitive impairment in psychiatry (SCIP-S) scale in patients with type I bipolar disorder" doc

Hóa học - Dầu khí

... those who did not, logistic regression analysis was Table 2: Mean SCIP scores for each of the parallel forms Form Form Form Subtest N Mean (SD) N Mean (SD) N Mean (SD) Significance VLT-I WMT VFT ... support for the feasibility, reliability, and validity of the SCIP as a tool for screening cognitive impairment in bipolar disorders, but some limitations should be taken into account A major ... genotype increases risk for bipolar I disorder and influences neurocognitive performance Bipolar Disord 2007, 9:370-376 Martínez-Aran A, Vieta E, Torrent C, Sánchez-Moreno J, Goikolea JM, Salamero...
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The financial reporting framework may not provide specific guidance for inclusion or exclusion of a docx

The financial reporting framework may not provide specific guidance for inclusion or exclusion of a docx

Kế toán - Kiểm toán

... requirements for separate opinions on components, thus indicating a need for lower materiality for the components Furthermore, certain procedures may be required to be performed by audit mandate or legislation, ... a basis for our qualified audit opinion Basis for Qualified Opinion The government’s expenditures for program XX is reported as USD 15 Millions in the consolidated income statement for the year ... Auditor P19 When communicating with the component auditor in accordance with paragraph 40 of ISA 600, public sector group auditors may find it relevant to provide the component auditors with information...
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báo cáo hóa học:

báo cáo hóa học:" Usefulness of five-item and three-item Mental Health Inventories to screen for depressive symptoms in the general population of Japan" ppt

Hóa học - Dầu khí

... item performed best as a detector of severe depressive symptoms, but each also contributed some information even for detecting moderate and mild depressive symptoms (Table 3) Both scales performed ... moderate, or severe depressive symptoms (ZSDS scores of 40 or higher) Moderate or severe depressive symptoms (ZSDS scores of 48 or higher) Severe depressive symptoms (ZSDS scores of 56 or higher) ... subjects, we used, instead, scores on the ZSDS For each of the three categories of the severity of depressive states (ZSDS scores of 40 or higher), we Page of (page number not for citation purposes)...
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righting the enterprise a primer for organizing or organizing the right way

righting the enterprise a primer for organizing or organizing the right way

Quản trị kinh doanh

... the work is or will be done; with WHO is performing or will perform the work; in a matrix of how the workers are or will be ORGANIZED to work together and be managed/ facilitated, and SUPPORTED ... results desired in support of work execution If, for example, you mandate that every manager fill out a form that has been designed for a performance review, a filled-out form can become the goal, ... Language of Work ModelTM, we use a variety of terminology to designate work, such as: outputs for deliverables, conditions for governances and work support for organizational support These have...
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Báo cáo khoa học:

Báo cáo khoa học: "Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog" pps

Báo cáo khoa học

... 30 cycles at 94oC for min, 55oC for min, 72oC for with an additional extension step at 72oC for 10 Part B Canine IL-6 cDNA amplification Preparation of canine blood cell A normal healthy adult ... initial denaturation at 94oC for followed by 30 cycles of amplification, each cycle consisted of denaturation at 94oC for min, annealing at 55oC for and extension at 72oC for with an additional final ... primers for vector ligation Using these primers, a 480 bp fragment, including the whole coding sequence of canine IL-3, was expected to be amplified The PCR amplification was performed for 30 cycles...
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Báo cáo y học:

Báo cáo y học: "Subcutaneous Immunoglobulin-G Replacement Therapy with Preparations Currently Available in the United States for Intravenous or Intramuscular Use: Reasons and Regimens" docx

Báo cáo khoa học

... initial attempts at IGIV therapy, or they may have been on insufficient doses or had their therapy interrupted before being referred to us for subcutaneous therapy For these patients and several ... types of adverse events Overall, for these patients, the requirement for frequent selfdosing results in less overall disease-related morbidity or interference with normal activities Onethird of the ... source of the information reviewed for this report 122 Allergy, Asthma, and Clinical Immunology / Volume 1, Number 3, Fall 2005 During this retrospective chart review, we recorded the reasons...
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Báo cáo khoa học:

Báo cáo khoa học: "Application of deltamethrin for spraying or dipping to protect Scots pine seedlings against Hylobius abietis L and logs against Tomicus piniperda L" ppsx

Báo cáo khoa học

... Decis 2.5 EC for dipping seedlings before planting or for spraying after planting in the plantation The experiments were carried out at the end of April in the Lochow and Myszyniec forest districts ... Plants are not protected by dipping before planting as forest planting work is sometimes carried out by children In 1987, experiments were carried out in the Forest Research Institute to compare ... plantations moved from the forest quickly enough and where the treatment capacities of the local forestry commission are limited We carried out a number of experiments in the Forest Protection Department...
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Báo cáo khoa học:

Báo cáo khoa học: "Results of emergency Hartmann''''s operation for obstructive or perforated left-sided colorectal cancer" ppt

Báo cáo khoa học

... colonoscopy and chest X-rays were performed routinely and abdominal CT scan or liver ultrasonography were performed in patients with raising CEA levels or clinical suspicion for tumour recurrence Whenever ... patients with T4 or N2 tumours, who are at high risk for developing local recurrence, are candidates for a definitive colostomy Page of (page number not for citation purposes) World Journal of ... of vogue"; it might be true for complicated diverticulitis, but probably not for the emergency management of left-sided colorectal cancer-the original indication for this time-honoured operation...
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Báo cáo khoa học:

Báo cáo khoa học: "High-dose rate brachytherapy (HDRB) for primary or recurrent cancer in the vagina" docx

Báo cáo khoa học

... ratio was taken to be 10 Gy for tumor effects and Gy for late effects The median EQ2 for the tumor was 66.48 Gy (31.25 – 75.4 Gy) for the entire group The median EQ2 for the late effects on vaginal ... guidance was used for tumors involving the vaginal apex to avoid injury to the bladder or adjacent small bowel CT simulation for interstitial brachytherapy planning was performed and the dose ... without any prior radiation and 4/6 (67%) for group with recurrence and any prior radiation therapy No grade or worse early toxicity was observed Two patients had late grade or morbidity One patient...
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báo cáo khoa học:

báo cáo khoa học: " Growth characteristics and lipid distribution in two lines of chicken selected for low or high abdominal fat" docx

Báo cáo khoa học

... observed in these lines, for example, for liver lipogenesis (Saadoun and Leclercq, 1987) or for implicated in the control of lipid metabolism like insulin or thyroid hor(Simon and Leclercq, 1982; ... between lines for abdominal fat proportion increased in both sexes until 63 d of age, then the difference remained constant At all ages genetic difference for abdominal fat proportion was more pronounced ... & Thornley J.H.M (1984) Growth functions In : Mathematical Models in Agriculture (J France and J.H.M Thornley eds.), Butterworth, Guildford, pp 75-94 Leclercq B (1988) Genetic selection for high...
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Immobilizing topoisomerase I on a surface plasmon resonance biosensor chip to screen for inhibitors pptx

Immobilizing topoisomerase I on a surface plasmon resonance biosensor chip to screen for inhibitors pptx

Báo cáo khoa học

... sensor chip for drug or protein interactions was successfully established Immobilization of biotinylated linear or circular DNA on the sensor surface for TopI and topII kinetic assays was performed ... and 1% Triton X-100) for 40 min, followed by electrophoresis in 1× TBE buffer at V/cm for 10 at room temperature After electrophoresis, slides were dehydrated in 70% alcohol for 20 and air-dried ... A DMSO calibration curve was included to correct for refractive index mismatches between the running buffer and inhibitor dilution series To correct for nonspecific binding and bulk refractive...
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báo cáo khoa học:

báo cáo khoa học: "Geometric conservation laws for cells or vesicles with membrane nanotubes or singular points" pdf

Báo cáo khoa học

... integral theorems lay A A the foundation for the conservation law for cells or vesicles with membrane nanotubes or singular points Figure A cell or vesicle with one membrane nanotube A cell or vesicle ... Derenyi I, Frenkel D, Dogterom M: Force barriers for membrane tube formation Phys Rev Lett 2005, 94:068101 Yin Y: Integral theorems based on a new gradient operator derived from biomembranes (Part ... Nanotubular highways for intercellular organelle transport Science 2004, 303:1007-1010 Evans E, Bowman H, Leung A, Needham D, Tirrell D: Biomembrane templates for nanoscale conduits and networks Science...
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