dehydrogenase—an enzyme of citric acid cycle
Tài liệu Báo cáo Y học: Evolution of the enzymes of the citric acid cycle and the glyoxylate cycle of higher plants pdf
... evolution of all of the enzymes of the pathway (Fig 4), it is clear that only about half of the enzymes of the tricarboxylic acid cycle, the major pathway of carbon metabolism in mitochondria of oxygen-respiring ... relatedness of several of the enzymes investigated to other enzyme families (Table 1) Higher-plant tricarboxylic acid cycle and glyoxylate cycle: eubacterial enzymes All of the plant enzymes surveyed ... origin of its individual enzymes However, the study of the enzymes of the tricarboxylic acid cycle necessarily also entails the study of the several enzymes involved in the glyoxylate cycle in...
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Báo cáo y học: "Inhibition of citric acid- and capsaicin-induced cough by novel TRPV-1 antagonist, V112220, in guinea-pig" potx
... Number of Coughs Number of Coughs 20 15 10 Pre-screen Citric Acid 25 Number of Coughs 25 25 V112220 (n=5) 20 15 10 Capsaicin Pre-screen Citric Acid Capsaicin 20 15 10 Pre-screen Citric Acid Capsaicin ... V112220 n=5 Figure Number 2of coughs following citric acid or capsaicin exposure Number of coughs following citric acid or capsaicin exposure Left panel show results from citric acid exposure while ... inhibition of cough induced by citric acid and capsaicin in the guinea-pig [14,15] Capsaicin Citric Acid 25 20 15 10 ** Control Vehicle n=4 n=5 V112220 n=5 Number of Coughs Number of Coughs 25...
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Báo cáo y học: " Kinetic modeling of tricarboxylic acid cycle and glyoxylate bypass in Mycobacterium tuberculosis, and its application to assessment of drug targets" ppt
... function of the amount of enzyme, any change in the amount of enzyme will affect the Vmax Therefore, varying Vmax for ICD1 from 1% to 100% was used to monitor the effect of inactivation of ICD1 ... Simulation of the effect of inhibition of both ICL1 and ICL2 Simulation of the effect of inhibition of both ICL1 and ICL2 by 3-nitropropionate (3-NP) Broken line represents the sum of flux through ... level (enzyme level in our study), there would be a proportional change in the Vmax of that enzyme (because Vmax is proportional to the amount of enzyme) Thus, using the Vmax values of enzymes...
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Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle ppt
... one of the key enzymes of the RTCA cycle, ATP-citrate lyase [24] The results demonstrated the heteromeric structure of this enzyme and its role in regulating the direction and flux of the RTCA cycle ... limicola, as a CO2-fixing enzyme in RTCA cycle The enzyme IDH from C limicola was revealed to be a monomeric enzyme with a molecular mass of 80.5 kDa The deduced amino -acid sequence of Cl-idh gene showed ... biosynthesis, under the condition of excess turnover of the RTCA cycle Further studies of the enzyme will clarify the structure, functions, and regulation in the RTCA cycle REFERENCES Evans, M.C.W.,...
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Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
... 3-hydroxyanthralinic acid differs from that in the pathway for 4-amino3-hydroxybenzoic acid The N-terminal amino acid sequence of the purified enzyme did not show significant levels of identity to sequences of 2-aminomuconate ... spectra of reaction products and the purified enzyme were recorded with a Beckman DU 650 spectrophotometer Fluorescence spectra of the purified enzyme and a cofactor released from the enzyme were ... coupled enzyme assay of 4-amino-3-hydroxybenzoic acid and the prepared crude extracts The absorption peaks at 263 and 294 nm characteristic of 4-amino-3-hydroxybenzoic acid decreased as the enzyme...
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Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx
... conformation of the enzyme in the absence of substrate and cofactor Molecular modelling To elucidate the different behaviour of these inhibitors on the two trypanosomatid gGAPDHs, modelling studies of enzyme inhibitor ... that improved activity of this compound may be a result of hydrogen bonding between the hydroxyl of Thr167 of the protein and the amino group of compound No other inhibitor offered such an interaction ... after preincubation of the enzyme with the compound for followed by addition of the reaction mixture to start the reaction A possible effect of the inhibitors on the absorbance of NADH was checked...
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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc
... is different in all of the tetrameric PDCs of known three-dimensional structure Binding of the cofactors ThDP and Mg2+ The homo-tetrameric IPDC binds four molecules of the cofactors ThDP and Mg2+ ... orientation of the aminopyrimidine ring, are conserved in all ThDP-dependent enzymes One of these, the hydrogen bond between the N1¢ atom of the pyrimidine ring of ThDP and the side chain of an invariant ... proteins involved in regulation of the gene expression of indole-3pyruvic acid decarboxylase, a key enzyme in indole-3-acetic acid biosynthesis in Azospirillum lipoferum FS Biosci Biotechnol Biochem...
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Tài liệu Báo cáo khoa học: Multi-targeted activity of maslinic acid as an antimalarial natural compound pdf
... proteolytic hydrolysis of haemoglobin as a source of amino acids constitutes one of the essential processes which take place along the intra-erythrocytic stage of the parasite Degradation of haemoglobin ... structure of the available inhibitors of cathepsin D [19], a lysosomal protease of mammalian cells, and chalcones and phenothiazines have been assayed as inhibitors of falcipain-2 [20,21] Inhibition of ... inhibition of falcipain [38] or by the indirect effect on the functionality of the vacuole as a result of the accumulation of partially hydrolysed peptides, leading to the accumulation of uncleaved...
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Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants doc
... redesign of the active site of the enzymes to elucidate the mechanism by which fidelity of amino acid selection is maintained We have focused our attention on a pair of species-specific enzyme variants, ... acid and l-aspartic acid to the enzyme [9,10] Detailed analysis of glutaminyl-tRNA synthetase has led to the proposal of an induced-fit type of active site rearrangement that plays a role in enzyme ... amino acid) ; 58 ([32P]-labelled tRNA) a ND, not determined because of the impracticality of using large amounts of [14C]-Cav jack bean enzyme Cav-tRNAArg was prepared by canavanylation of the...
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Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt
... stabilization of EcIPDC after addition of the cofactors The enzyme is destabilized at low ionic strength The stability of EcIPDC in aqueous solutions is higher than that of other PDCs The rate constant of ... N-terminal amino acid sequence of the purified enzyme (Met-Arg-Thr-Pro-Tyr-Cys-Val-Ala) is identical to that of the nucleotide sequence of the EcIPDC gene (DCIP_ENTCL) Fig pH dependence of the oligomeric ... integral of with respect to the indole protons is consistent with the occurrence of the enol form of indolepyruvate (Fig 1) In the course of the establishment of the equilibrium % 85% of the enol...
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Báo cáo Y học: Characterization of the exopolysaccharide produced by Streptococcus thermophilus 8S containing an open chain nononic acid doc
... spectrum (mixing time 300 ms) of n-EPS, recorded in D2O at 64 °C Diagonal peaks of the anomeric protons, of H-4 of residues A and C, H5 of residue E, and H-3¢a,3¢b,9¢ of residue D are indicated ... monosaccharide composition of Gal, Rib and Glc in the molar ratio of : : (GLC analysis), the deamination product of GalpNAc (2,5-anhydro-Tal-ol-1-d) in a molar ratio of in terms of peak areas compared ... arising from the loss of water due to the formation of an intraresidual lactone in the nononic acid part of Sug [10] The tandem mass spectrum obtained on collision activation of the pseudomolecular...
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Báo cáo khoa học: The 2-oxoacid dehydrogenase multi-enzyme complex of the archaeon Thermoplasma acidophilum ) recombinant expression, assembly and characterization docx
... pyrophosphate (TPP)-dependent decarboxylation of the 2-oxoacid and the transfer of the resulting acyl group to the lipoic acid of E2 E2 then transfers the acyl-group to coenzyme-A (CoASH), after which E3 ... attachment of a C-8 intermediate of fatty acid biosynthesis to the target lysine on E2 by enzyme LipB; subsequently, LipA catalyzes the incorporation of sulfur atoms to generate the lipoic acid moiety ... assembled enzyme complex were pre-incubated at 55 °C for 10 to allow binding of TPP to E1 The assay, in a final volume of mL, was started by the addition of the 2-oxoacid substrate as for the assay of...
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Báo cáo khoa học: Discovery of GSK837149A, an inhibitor of human fatty acid synthase targeting the b-ketoacyl reductase reaction pot
... inhibitor of human fatty acid synthase Enzymatic mode of inhibition Table Summary of inhibition patterns caused by GSK837149A on the KR reaction catalyzed by FAS With the aim of nding the mode of inhibition ... therapeutic application of this chemical family, the relevance of the inhibitors is still noticeable because of their high-afnity binding to the enzyme and the novelty of their mechanism of action It is ... inhibitor of fatty acid synthase Proc Natl Acad Sci USA 97, 34503454 Rendina AR & Chend D (2005) Characterization of the inactivation of rat fatty acid synthase by C-75: inhibition of partial...
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Báo cáo khoa học: Kinetics of intra- and intermolecular zymogen activation with formation of an enzyme–zymogen complex ppt
... irreversible step of pepsinogen activation Biochemistry 28, 2626–2630 33 Darvey IG (1977) Transient phase kinetics of enzyme reactions where more than one species of enzyme is present at the start of the ... Havsteen BH, Garcı´ a-Moreno M & Vazquez A (1992) Kinetics of a model of autocatalysis, coupling of a reaction in which the enzyme acts on one of its substrates J Theor Biol 154, 261–270 ´ ´ Varon ... route of intramolecular activation of the zymogen into the active enzyme, E, and one or more peptides represented by W [route (a), Scheme 3] [12,22,25–27] and a route of autocatalytic activation of...
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Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx
... 27 acids ⁄ 14 acids ⁄ 14 acids ⁄ 14 acids ⁄ 14 acids ⁄ 14 amino amino amino amino amino 15 15 15 15 15 acids acids acids acids acids amino amino amino amino amino acids ⁄ 237 acids ⁄ 237 acids ... Obtaining 5¢ and 3¢ ends by Rapid Amplification of cDNA Ends (RACE) amino amino amino amino amino acids acids acids acids acids Relative molecular mass ⁄ pI of mature protein homology search in GenBank ... carboxylate of this Asp217 (194, chymotrypsin numbering) is involved in the formation of a salt bridge with the N-terminal Ile of the mature enzyme for completing the formation of Fig Distribution of...
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Báo cáo khoa học: The 3-ureidopropionase of Caenorhabditis elegans, an enzyme involved in pyrimidine degradation pptx
... (N-Carbamyl-b-aminoisobutyric acid) 4-Amino-4-oxo-butyric acidb (succinamic acid) 2-Ureidoacetic acid (N-carbamylglycine) 4-Ureidobutanoic acid (N-carbamyl-c-aminobutyric acid) 2-Ureidopropionic acid (N-carbamy-a-alanine) ... h), the half-life of enzymatic activity was only 65 s The activity of the enzyme remained stable at a specic activity of 0.2 Uặmg protein)1 after of preincubation The dependence of the activity ... shown of 3-ureidopropionase of C elegans and plants It is quite unexpected that the Km of the D melanogaster enzyme is approximately six-fold lower because both enzymes show a high degree of sequence...
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Báo cáo Y học: The effect of amino-acid substitutions I112P, D147E and K152N in CYP11B2 on the catalytic activities of the enzyme pdf
... investigated whether the amino acids of human CYP11B2 located in regions aligned with these helices are of functional importance They were replaced by the corresponding amino acids of human, mouse and rat ... alignment of the cytochromes P450 with known structure; (b) determination of structurally conserved regions (SCRs) of the proteins based on this structural alignment; (c) building of the backbone of ... cytochrome P450 [23], and as the conversion of a multitude of compounds might be due to the high variability in the SRS of the family P450s [35], amino acid 112 of CYP11B1 and CYP11B2 may therefore...
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Báo cáo Y học: Production and chemiluminescent free radical reactions of glyoxal in lipid peroxidation of linoleic acid by the ligninolytic enzyme, manganese peroxidase pot
... the initial rate of increase in absorbance at 470 nm in the presence and absence of manganese One unit of enzyme activity is defined as the amount of enzyme that oxidizes mmol of 2,6-DMP in Laccase ... instead of linoleic acid (E) As (A) but 1-hexanal was added instead of linoleic acid (F) As (A) but 2,4-nonadienal was added instead of linoleic acid (G) As (A) but MDA was added instead of linoleic ... instead of linoleic acid (D) As (A) but 1-dodecanal was added instead of linoleic acid (E) As (A) but 1-hexanal was added instead of linoleic acid (F) As (A) but 2,4-nonadienal was added instead of...
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Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot
... length) The requirement of cofactor for TDP enzymatic activity was examined by determining the specific activity of TDP by following the cleavage of mM of substrate by 0.125 lM of enzyme in reaction ... dependence of human TDP enzyme activity The reactions were carried out in a total volume of 200 lL of 50 mM Tris/HCl, 100 mM NaCl, mM Mn2+, 0.125 lM of human TDPND39 enzyme, and mM of substrate ... obligatory homodimeric enzymes, the specific activity of human TDP stays constant and is independent of enzyme concentration, as expected for a monomeric enzyme TDP being a monomeric enzyme is corroborated...
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Phân tích tỷ lệ tăng giá thuê tai cac giai đoạn khác nhau trong chu kỳ của thị trường bất động sản "An analysis of rental growth rates RE market cycle"
... down -cycle back to 4.4% The standard deviation of absorption did increase in the up -cycle and decline in the down -cycle (see exhibit 2) Office completion rates were high at the bottom of the cycle ... through the rest of the down -cycle (see exhibit 3) Office rental growth was negative in cycle positions one and two then was low but positive through the rest of the recovery phase of the cycle Beginning ... the up -cycle from a low of -0.3% to a high of 3.6% and then fell during the downcycle back to 0.1% The standard deviation of absorption did increase in the up -cycle and decline in the downcycle...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu