d0 associated with early death in the icu £ 48 h
Báo cáo y học: "Mechanical ventilation using non-injurious ventilation settings causes lung injury in the absence of pre-existing lung injury in healthy mice" pdf
... about in the high VT or high pressure group [2,30] Worth mentioning is that VT or pressures used in the high VT group in these former studies were about twice as high as in our study protocol In a ... ketamine (Eurovet Animal Health B.V., Bladel, the Netherlands), mg/ml medetomidine (Pfizer Animal Health B.V., Capelle a/d IJssel, the Netherlands) and 0.5 mg/ml atropine (Pharmachemie, Haarlem, the ... reflected the human setting by using clinically relevant VT, preventing shock and gross lung histopathological changes, and compared lower VT with higher VT with respect to several endpoints of VILI In...
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Báo cáo sinh học: "Genetic parameters for lactation traits of milking ewes: protein content and composition, fat, somatic cells and individual laboratory cheese yield" ppt
... longitudinal cutting and the whey being removed after draining for 45 with the test-tube face downward (the curd remained quiescent at the bottom of the tube) ILCY was dened as the weight of the centrifuge ... ewes These correlations depend, in part, on the degree of infection within the population that we are dealing with In fact, in spite of the strict mastitis control measures and the general high ... reduced milk amounts employed and the forced draining (centrifugation) of the cottage cheese, which captures even the smallest curd that may be lost with the whey in the industrial process 3.1 Environmental...
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Báo cáo y học: "MALDI-TOF MS Combined With Magnetic Beads for Detecting Serum Protein Biomarkers and Establishment of Boosting Decision Tree Model for Diagnosis of Colorectal Cancer"
... lower than or equal to the threshold, this peak would go to the left-side child node Otherwise, the peak would go to the right-side child node After rounds of decision making, the training set ... ProteinChip array After air-drying for minutes at room temperature, protein crystals on the chip were scanned with the ProteinChip (Model PBS IIc) reader (Ciphergen) to determine the masses and intensities ... separator for min, after which the supernatant was discarded carefully by using a pipette The magnetic beads were then washed twice with 100 μl binding buffer Then 100 μl of the diluted serum...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... for the specific MAP2 binding resides within the 32 residues (amino acids 456 487 ) of the v-KIND KIND2 domain To examine whether the MAP2 binding core site of v-KIND binds to intact MAP2 protein, ... The residues conserved across all species in the KIND2 domain and in any other KIND domain are highlighted in gray, whereas those conserved only in the KIND2 domain are highlighted in black The ... suggesting that the core sequence critical for the specific v-KIND KIND2 binding resides within residues 702–744 of MAP2 Residues 456 487 in the KIND2 domain of v-KIND contain the core MAP2 binding module...
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Tài liệu Báo cáo khoa học: SREBPs: protein interaction and SREBPs Ryuichiro Sato doc
... lipoprotein protein The basic helix–loop-helix leucine zipper domain in SREBPs binds to the ligand-binding domain in LRH-1, thereby reciprocally suppressing their transcriptional activities SREBPs interfere ... interfere with the recruitment of a coactivator of LRH-1, PGC-1a, resulting in the inhibition of LRH-1 activity When human hepatoma HepG2 cells are cultured with an HMG-CoA reductase inhibitor, statin, ... quite characteristic transport pathway, in that the nuclear import occurs in the absence of importin-a Furthermore, the dimerization of SREBPs via the leucine zipper domain is required for the interaction...
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Tài liệu Báo cáo khoa học: Combinatorial approaches to protein stability and structure pdf
... [88,89] The overpacking was accommodated primarily by a main-chain shift of the C-terminal helix away from the helices that contain the mutations, with the ˚ largest movements on the scale of A The ... wild type This seems to highlight the importance of packing complementarity and the stereochemical nature of the constraints on that packing However, the fact that the architecture of the repressor ... NOESY, 15N- 1H HSQC and 13C- 1H HSQC NMR spectra indicated that four of the five proteins had well-ordered and persistent main-chain and sidechain structure The best of these was shown to have a substantial...
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Tài liệu Báo cáo khoa học: The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins pdf
... domain in the processing of membrane-tethered mucins The mechanism by which the heavily glycosylated portion of these molecules is released at epithelial surfaces within the airway, the intestine ... even when substituted into another mucin The cleavage is apparently more efficient in the hybrid mucins carrying the whole SEA domains rather than the 33 amino acid fragments encompassing the G ... previously The fragments were then cloned into the SpeI ⁄ XhoI sites of the polylinker in MUC1FDTR The remaining portions of the polylinker introduced leucine and valine residues N-terminal to the MUC3...
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Tài liệu Báo cáo Y học: Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes pot
... against synthetic phosphopeptides corresponding to p70S6K phosphorylation sites containing phosphoThr389 (anti-pThr389), phosphoSer-411 (anti-pSer411), or phosphoThr421 together with phosphoSer424 ... of the cells with glutamine or glutamine plus leucine correlated with the increase in p70S6K activity brought about by these amino acids (Fig 6, lanes 1–4) The data also show that increases in ... dephosphorylation Taken together, these data support the hypothesis that two phosphatases are involved, one upstream of mTOR, the activation of which is required for the activation of both ACC...
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Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx
... [28] These findings agree with the hypothesis [29] that an increase in the mitochondrial membrane potential would slow the transport of electrons through the respiratory chain, increasing the time ... through a mechanism proposed to involve higher production of intracellular ROS [14] On the other hand, studies in cells overexpressing UCP2 have reinforced the belief that UCP2 plays a role in ... 0.05) in MDP-treated cells Interestingly, the OÀ level decreased after h of stimulation, returning almost to the resting level after h On the other hand, stimulation with MB failed to induce...
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Báo cáo khoa học: Concepts and tools to exploit the potential of bacterial inclusion bodies in protein science and biotechnology pdf
... of the quality control system upon the accumulation of misfolded proteins in the cytoplasm suggest that this mechanism shares similar features with the heat-shock response, which causes the upregulation ... residues of the functional domain of the HA2 subunit of the in uenza virus hemagglutinin protein and to detect conformational heterogeneity of the protein within IBs [65] NMR spectroscopy has also ... binding domain did not interfere with the correct folding of the partner protein that aggregated in a form endowed with biological activity (in the case of DAAO this means also ability to bind...
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Báo cáo khoa học: Protein aggregation and amyloid fibril formation prediction software from primary sequence: towards controlling the formation of bacterial inclusion bodies pot
... Several other parameters are calculated and reported, such as the average a4v in each hot-spot, the area of the aggregation profile above the HST, the total area (the HST being the zero axis) and the ... contacts higher than the cut-off) and the size of the region is greater than or equal to the size of the sliding window used Optimum values for the cut-off (threshold) and the sliding-window length ... averaged inside the window and the average is assigned to the central residue of the window The ‘smoothed’ expected values for every position of the polypeptide chain provide the final profile, which...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... Fig ITC of ATP binding to GlnK The raw data were fitted with a three-site binding model for a PII trimer The upper panel shows the raw data in the form of the heat effect during the titration of ... binding to the His6-TnrA surface, the analyte was preincubated for on ice with the various effector molecules as indicated, and was then injected into the sensor chip For novel reloading of the ... GlnK–TnrA interaction in the presence of mm ATP On the other hand, 2-oxoglutarate did not in uence the GlnK–TnrA interaction, either alone, in the absence of divalent metals, or in combination with ATP...
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Báo cáo khoa học: Protein–protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling doc
... Ras signaling [36–38] These systems employ the cdc25 yeast strain, which is deficient in Ras signaling and regains it with the presence of interacting protein pairs The machinery of intrinsic cell ... that the specific interaction of the receptor with the C-terminus of Ga is necessary to bring the two proteins into close proximity This hypothesis was applied to the analysis of protein–protein ... signaling on the basis of delay of entry into the logarithmic growth phase The agar diffusion bioassay (halo assay), in which cells are mixed with unsolidified fresh agar medium in which pheromone-spotted...
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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... framework with hypervariable sequences and lengths in the six-loop CDR (Fig 1A) that bind to the antigen The first study of grafting into the CDR replaced the CDR loops in a human antibody with those ... Comparison of the X-ray crystal structures between charybdotoxin and toxin a showed the structural resemblance of the b-hairpin loop with binding function between toxins The grafting of the toxin a loop ... small binding proteins with binding function further allows us to increase the binding strength by multibinding approaches, constructing multispecific proteins from two small proteins with different...
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Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot
... effective in the binding of the ligand to the other repeats that share a common epitope Other inhibition mechanisms cannot be excluded; among these, there is the possibility that, through the effect ... note that the poor conservation of this stretch in the other repeat units of both adhesins is consistent with the marginal reactivity of the repeats to 15E11 Overall, these data confirm that the ... effect of the proximities of the antibody-binding and Fn-binding sites, the interaction of the repeat with Fn may be sterically hindered in the presence of the antibody The results of this study...
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Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt
... the side chain of Arg78 (Hg) and Glu82 (Hc1) and the medium-range NOE between the side chains of Asp81 (Hb) and Arg78 (Hg and Hc)] In addition, Glu82 interacts with the side chain of Lys75 The ... against the virus, cross-reacts with the human tissue to cause autoimmune disease An interesting observation is the structural organization of the fragment 76–84 of the Vb chain of the TCR with ... that have a single, fully conserved residue; Ô:Õ indicates that one of the HRK (histidine-arginine-lysine) groups is fully conserved; Ô.Õ indicates that one of the STP (serine-threonine- proline)...
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Báo cáo khoa học: "Age Prediction in Blogs: A Study of Style, Content, and Online Behavior in Pre- and Post-Social Media Generations" ppt
... Twitter each coincide with only one outlying year which suggests that either they had less of an impact on writing style or, in the case of Twitter, the change has not yet been transferred to other ... which is the number of posts the term occurred in Then, similarly to interests, we pruned each list to include the features that did not occur within +/-10 window (based on its position in the ... position in the list) in multiple age groups We show the top interests in each age group in Table For example, “disney” is the most popular unique interest in the 18-22 age group with only 39 other...
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Báo cáo khoa học: NBR1 interacts with fasciculation and elongation protein zeta-1 (FEZ1) and calcium and integrin binding protein (CIB) and shows developmentally restricted expression in the neural tube pptx
... conserved, with 87% sequence similarity with the murine homologue The murine Nbr1 gene lies head to head with the Brca1 gene, with the intragenic region of 289 bp suggesting the possibility of co-ordinated ... the C-terminal amino acids 370±392 (Y163) Clone Y198 encoded the full-length cDNA of the calcium and integrin binding protein (CIB) in frame with the GAL4 AD Mapping of regions involved in the ... describe the identi®cation of two proteins that interact with NBR1; CIB and FEZ1, which were also shown to interact with each other CIB was itself cloned by a yeast two-hybrid study using the integrin...
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Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant Mark Wehner and Christian Herrmann doc
... investigate the thermodynamics of nucleotide binding Using this method, it is possible to determine the stoichiometry, enthalpy change, dissociation constant and thereby the change in entropy in a single ... (taking elongated shape into account) The size of the protein complex is only slightly increased when nucleotide-free or in the presence of GTP As with hGBP5ta, the complex is increased to the ... monomers Human guanylate binding protein (hGBP5) belongs to the family of interferon-c-induced p65 GTPases, which has seven members in the human genome [5] This family of guanylate binding proteins...
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Báo cáo khoa học: pyr RNA binding to the Bacillus caldolyticus PyrR attenuation protein – characterization and regulation by uridine and guanosine nucleotides potx
... three points The horizontal dotted line shows the concentration of non-binding RNA from Fig 4D, in which the RNA peak is clearly defined The intersection between the dashed and dotted lines indicates ... for RNA than the other The high affinity state is favored by binding of either uridine nucleotides or binding of RNA itself in the case of BcBL1 The low affinity state is favored by the binding of ... of 0.5 mm UMP, which stimulated binding for all three binding loops, RNA binding to PyrR PyrR binding to BcBL1 resembled the binding observed for the other two binding loops The apparent dissociation...
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Tìm thêm: khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25