c— structural organization of the major gallbladder mucin muc5b
Báo cáo khoa học: Role of the N- and C-terminal regions of the PufX protein in the structural organization of the photosynthetic core complex of Rhodobacter sphaeroides pptx
... neither affects the photosynthetic capability of the bacteria nor the efficiency of exchange of the ubiquinol molecules between the RC and the bc1, as judged from the reduction kinetics of cytochrome ... ambiguous the attribution of the PufXD2)19 strain to the wild-type or the PufDX cluster Isolation of the photosynthetic complexes from the mutant strains As described previously [16], the photosynthetic ... suppose that the discrepancy is related to the presence of the LH2 which could influence the shortening processes of the assembled PufX protein The exchange of ubiquinone between the RC and the cyt...
- 9
- 547
- 0
Báo cáo khoa học: Mapping of the 45M1 epitope to the C-terminal cysteine-rich part of the human MUC5AC mucin potx
... of the epitopes for the mAbs, where 45M1 recognizes an epitope located in the N-terminal region of the C-terminal cysteine-rich part of MUC5AC, presumably in the last CysD domain of the mucin The ... cysteine-rich part Further localization of the epitopes of 45M1, 2-12 M1 and 166M1 In order to further locate the epitopes of the hitherto unmapped 45M1, 2-12M1 and 166M1, these mAbs were screened ... to direct the protein synthesis into the secretory pathway L31 [21], the cDNA clone used in previous studies aimed at mapping the epitopes of the mAbs against M1 to the C-terminal part of MUC5AC...
- 9
- 330
- 0
Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx
... value of the mutant TNSALP was less than one-tenth of the Kcat ⁄ Km value of the soluble form of the wild-type enzyme, indicating that the replacement of valine with alanine at position 406 in the ... of TNSALP In support of our hypothesis, the cysteine residue at position 433 in one subunit of TNSALP (R433C) becomes crosslinked to the counterpart of the other subunit [29], implying that the ... containing the SV40 early promoter [11] Shortage of a precursor of GPI in the endoplasmic reticulum may be one of the reasons why a small but significant fraction of the wild-type TNSALP forms the aggregate...
- 11
- 500
- 0
Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx
... parameters, there is no relationship between S2 and the location of structural elements (Fig 4) Furthermore, the Rex terms are distributed throughout the 3D structure of the protein, and most of them ... Furthermore, the internal motions of each bond vector are independent of each other, but the rotational diffusion of the molecule affects each of those bond vectors identically [42,43] On the other ... responsible, from a thermodynamic point of view, for the binding of the monomeric species of CAC? We have previously discussed the variation in the free energy of binding as a function of the changes...
- 13
- 421
- 0
Báo cáo khoa học: Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron-encoded protein contribute to its maturase and intron ppt
... Effect of mutations in the C-terminal region of the RmInt1 IEP on RNA splicing in vivo We constructed a series of mutants to identify the functional features of the C-terminal region of the RmInt1 ... DIVa of the intron, whereas other regions of the RT and domain X interact with conserved catalytic core regions [12] Domain X is located in the position corresponding to the ‘thumb’ and part of the ... residue at the N-terminus of a-helix a4, the domain X contains the conserved amino acid residues HKXRA (RmInt1 residues 388–392) carrying a stretch of basic amino acids Some of the residues of the HKXRA...
- 11
- 398
- 0
Báo cáo khoa học: Structural flexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for specific substrate recognition pptx
... fold of one monomer is positioned above the catalytic core of the other Interestingly, Arg267 from the beginning of helix in the HTH fold of one monomer is positioned towards the active site of the ... nucleophilic attack of the a-phosphate of ATP Thus, according to the crystal structure, the specificity of serine recognition depends on: (a) the zinc ion, (b) the size of the active site and (c) the hydrophilic ... that the positioning of the cognate tRNA in the active site of mMbSerRS would be facilitated upon the conformational change of the motif loop, which was the only proximal protein region in the...
- 14
- 357
- 0
Báo cáo khoa học: Sequences and structural organization of phospholipase A2 genes from Vipera aspis aspis, V. aspis zinnikeri and Vipera berus berus venom Identification of the origin of a new viper population based on ammodytin I1 heterogeneity docx
... Ib in the genome of this snake These findings provide clues to the evolutionary position of the neurotoxic V a aspis population with respect to the other snakes studied The genome of one of these ... with the neutral evolution theory, which states that the strong conservation of exons serves to maintain the function of the mature protein [26] The protein-coding regions of the PLA2 genes of ... and the 5¢ part of exon encode the mature protein; exon encodes the 5¢-UTR, and exon and part of exon encode the signal peptide The 110 nucleotides at the 3¢ end of exon encode the 3¢-UTR The...
- 10
- 451
- 0
Báo cáo khoa học: Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identification of sulfated high-mannose type oligosaccharides doc
... majority of the sulfated sugar In conclusion, the results obtained provide evidence of the nature of the glycans present in the unique N-glycosylation site present in the C-terminal domain of cruzipain ... Altogether, these data confirm the presence of sulfated high-mannose type oligosaccharides in cruzipain and in its C-terminal domain This is the first report of the use of nor-harmane as matrix for structural ... to characterize the N-linked glycans present in the protein In this paper we report, for the first time, the presence of sulfate in the N-linked oligosaccharides of cruzipain This structural feature...
- 13
- 536
- 0
Báo cáo khoa học: Phosphatidylserine induces functional and structural alterations of the membrane-associated pleckstrin homology domain of phospholipase C-d1 docx
... change of the PH domain at the membrane interface contributes to the change of the membrane binding affinity induced by PS The remarkable effect of low concentrations of MgCl2 on the formation of the ... affects the affinity for binding to the PC ⁄ PIP2 membrane in the absence of PS These suggest that the lowering effect of PS on the membrane binding affinity of the PH domain is mediated by the electrostatic ... Electrolytes suppress the effects of PS on the PH domain As shown in Fig 2B, the addition of 25 mm NaCl causes a decrease in the intensity of the Ala88 peak at 17.48 p.p.m in the spectrum of the PH domain...
- 11
- 425
- 0
structural and functional organization of the synapse
... deciphering the structural and functional organization of the synapse We have assembled leaders in the field of synapse biology to describe and distill the wonders and mysteries of the synapse ... (47) Only the frequency of spontaneous events declines as the expression of VGLUT drops below 10–20% of the wild type level The loss of miniature events presumably reflects the exocytosis of “empty” ... STRUCTURAL AND FUNCTIONAL ORGANIZATION OF THE SYNAPSE STRUCTURAL AND FUNCTIONAL ORGANIZATION OF THE SYNAPSE Edited by Johannes W Hell University of Iowa Iowa City, IA,...
- 807
- 506
- 0
Báo cáo y học: " A sequence-based survey of the complex structural organization of tumor genomes" pdf
... assay the other clones in the BES cluster with 69F1 Each of the other clones in the cluster are indicated as lines below 69F1, with the end-points of the lines indicating the locations of the mapped ... score [29] of the SNP, the mean phred score of the five bases centered on the SNP, and the mean phred score of the entire BES containing the SNP all must exceed 30 Approximately 58% of the candidate ... if the locations of the variant overlapped the locations of an end sequence pair in the cluster, and the type of variant was consistent with the orientations of the mapped end sequences in the...
- 17
- 424
- 0
Organization of the study
... with an advantage even under these conditions, such as the minimization of the risk of loss, the maximization of the chance of gain, or the minimization of the regret the decision maker may suffer ... implementation of the laws necessary for the reform of the economic system These include the National Assembly (NA), the country's legislature; the Standing Committee (SC) of the National Assembly, ... low that there were three commercial banks who offered loan to the JV company These banks might foresee the profitability of the project They therefore, did not hesitate to provide their quotations...
- 76
- 518
- 1
Tài liệu Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc
... available regarding the phospholipid and membrane binding of the other members of the PEBP family Consequently, the binding characteristics of PEBP proteins, including those of the binding of IC to phospholipid ... fluorescence of IC–GFP was observed at the FM4-64stained vacuolar membranes and also the vacuolar 5378 lumens in the majority of yeast cells (70% of the cells grown at 72 h; right panels of Fig 3A,B) Therefore, ... centrifugation of the samples, proteins bound to liposomes were analyzed by SDS ⁄ PAGE of the resulting pellets (C) The amounts of IC in the pellets The amounts of IC were quantitated with the UN-SCAN-IT...
- 10
- 645
- 1
Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt
... type of KLK11, isoform 2, despite the presence of exon 1c The secretory pathways of KLK11 isoforms appeared to differ, although both isoforms were secreted from cells Hence, the promoter use of the ... the isoform of translation products through the determination of the first exon in KLK11 mRNA (summarized in Fig 2A) Detection of the first exon in each KLK11 mRNA reflects the promoter use in the ... study the tissue-specific use of each promoter We show the translation initiation site of isoform and different intracellular distribution of isoforms and 2, and discuss the regulatory mechanism of...
- 9
- 544
- 0
Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
... amount of time At the end of the heat treatment the mixtures were placed in an ice/water bath, and the enzymatic activity was stopped by lowering the pH of the reaction mixture to by addition of ... during the thermal treatment, hydrolysis of BLG was extensive, in spite of significant inactivation of the enzymes This indicates that the transient conformers originating in the course of thermal ... in these preparations could stem from the inability of the hydrolytic reaction to address all the sequential epitopes even in the denatured protein In fact, despite the apparent simplicity of the...
- 11
- 526
- 0
Tài liệu Báo cáo Y học: Interallelic complementation provides genetic evidence for the multimeric organization of the Phycomyces blakesleeanus phytoene dehydrogenase doc
... sequence analysis of the carB mutant alleles To check if strain A486 was altered in the carB gene, and in order to get further insights into the nature of the mutations of the carB gene in strains ... membrane-bound enzymes In Phycomyces the analysis of the deduced amino-acid sequence encoded by the carB gene reveals the presence of a transmembrane region near the C-terminus of the protein [17] De®ciencies ... found in addition to colonies showing the colour of each of the two parental strains involved in the construction of each heterokaryon (data not shown) Therefore, the mutation in strain A486 complemented...
- 7
- 479
- 0
Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf
... 2001 cleavages of the sites were in the order: Tyr146/Asn147 ! Phe114 ! other sites The weakness of bands of fragments I and IV and the appearance of fragment II in the degradation of the Tyr146 ... in the rate of cleavage of the autolysis loop At the same time the amount of fragment III was similar to that observed in the degradation of D-chymotrypsinogen (panel a) showing that the rate of ... cleavage was the same as seen from their inactivation rates in the presence of equimolar amounts of trypsin (Table 2) The cleavage of the interdomain and autolysis loops To follow the autolysis of D-chymotrypsin...
- 9
- 613
- 0
Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... activity of the enzyme was determined using the Betamyl b-amylase test reagent (Megazyme, Wicklow, Ireland) To determine the pH stability of the b-amylase, aliquots of a solution of the purified ... for h at 25 8C Then 0.1 vol of a solution of 0.5 M sodium acetate (pH 5.0) was added and the activity of the enzyme was measured by the dinitrosalicylic acid method Inhibition of the enzyme activity ... at their 50 and 30 boundaries, respectively, and were inserted between the third letter of one codon and the first letter of the following codon Molecular modeling of the C sepium b-amylase The...
- 11
- 611
- 0
Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right of the figure indicate the positions of the nucleotide sequence The numbers on the left of the ... has been suggested to be the result of degradative reactions, rather than imperfect biosynthesis [13] Of the plant allergens listed in the of cial allergen database of the IUIS, about 25% belong ... to 230 proteins and about 65 of these are IgE binding proteins [20]; however, some of these are known to be either isoforms or the degraded products of allergens Furthermore, allergic patients...
- 10
- 665
- 0
Báo cáo khóa học: Structural properties of the protein SV-IV potx
... the total number of charges measured by electrospray MS Theoretical mass is the mass calculated on the basis of the protein amino-acid sequence The relative abundance refers to the ratio of the ... acetylation at one of the three residues, because the signal of the triacetylated species was less intense than that of the diacetylated species The MS/MS Ó FEBS 2003 Structural properties of SV-IV (Eur ... the mass increase of 42 mass units The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species From the data summarized...
- 9
- 416
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose