c— additional crystal forms in bile
Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx
... factors, including IFN regulatory factor-3 (IRF-3) and kinases, including the Tank-binding kinase (TBK-1) and inhibitor of jB kinase e (IKK-e) [28–31] Although IRF-3 is located in the cytoplasm in ... glycoproteins This response initiates signaling pathways leading to the induction of protective cellular genes, including type-I interferons [initially interferon (IFN)-b, and then IFNa] and proinflammatory ... clearly induced in PH5CH8 and HuH-7 cells, and TLR3 mRNA was induced only in PH5CH8 cells Moreover, there was no such induction in the other cell lines examined (supplementary Table S1) In M-pIC...
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Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells pdf
... expressed intracellularly This may be due to the reducing conditions existing in cell compartments, which may prevent the formation of intradomain disulfide bonds within the VH and VL domains resulting ... fused to d1-EGFP are maintained in the cells by binding to antigen Generation of soluble binding-defective and cysteine-deleted mutants To demonstrate that the observed intracellular colocalization ... was obtained with scFv 4A-d1-EGFP and 7A-d1-EGFP expressed in parallel Interestingly, in the case of 4A (but not 7A), we found a minor percentage of cells with a faint cytoplasmic staining (arrow)...
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Báo cáo khoa học: Assembly of nuclear DNA-encoded subunits into mitochondrial complex IV, and their preferential integration into supercomplex forms in patient mitochondria doc
... subunits were individually incubated with isolated fibroblast mitochondria for increasing times as indicated Samples were treated with proteinase K, and solubilized in either (A) DDM-containing buffer ... 35S-labeled Cox6a for increasing times in the presence or absence of a membrane potential (Dwm) Each sample was treated with proteinase K before being solubilized in digitonin-containing buffer and ... proteinase K before being split in two and (A) solubilized in DDM-containing buffer and subjected to BN-PAGE (protease-treated samples only), or (B) SDS–PAGE and phosphorimaging The right panels in...
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Báo cáo khoa học: Respective roles of the catalytic domains and C-terminal tail peptides in the oligomerization and secretory trafficking of human acetylcholinesterase and butyrylcholinesterase potx
... Role of cysteines in oligomerization – effects of introducing an additional cysteine In a previous study, we found that mutating residue 19 in the t peptide of Torpedo AChE considerably increased ... protein–protein interactions [45], because their catalytic domain is homologous to adhesion proteins such as neuroligin [46,47] For example, AChE may be involved in neurite extension during brain ... Mouse AChE binds in vivo to domain IV of laminin-1beta Chem Biol Interact 157 ⁄ 158, 411–413 46 Krejci E, Duval N, Chatonnet A, Vincens P & Massou´ lie J (1991) Cholinesterase-like domains in enzymes...
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Báo cáo khoa học: C fi G base mutations in the CArG box of c-fos serum response element alter its bending flexibility Consequences for core-SRF recognition potx
... 70° sharp bend induced in the HMG box seems very interesting Indeed, the bend determined by Raman for the free SREfos in solution is roughly similar to that formed in SREfos in the crystal of its ... duplexes in a temperature region where the premelting is dominant By contrast, V2 is mainly normalized according to melting and weak variations between the three duplexes can be seen during premelting ... and an increase in the intensity of its 790 cm)1 component, thus expressing changes in the geometry of the phosphodiester group, and ⁄ or in the conformation of deoxycytidine or deoxythymidine,...
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Báo cáo khoa học: Kinetic deuterium isotope effects for 7-alkoxycoumarin O-dealkylation reactions catalyzed by human cytochromes P450 and in liver microsomes Rate-limiting C-H bond breaking in cytochrome P450 1A2 substrate oxidation pdf
... secondary kinetic isotope effects [19,27–29] The existence of a gem secondary kinetic isotope effect has the effect of attenuating the rate of C-H bond-breaking, so in principle the intrinsic kinetic ... effects are interpreted to mean that the step(s) involving the chemistry of C-H bond cleavage are ratelimiting in the reaction even in liver microsomes, as opposed to steps involving generation ... values of the intramolecular values in Table are reasonable but should be considered lower estimates of the intrinsic kinetic deuterium isotope effects Intermolecular competitive kinetic isotope...
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Báo cáo khoa học: Differential involvement of protein kinase C alpha and epsilon in the regulated secretion of soluble amyloid precursor protein docx
... NaCl/Pi containing 3% hydrogen peroxide and 10% methanol for 15 min; nonspecific binding with PKCa and PKCe was blocked by incubation for 30 with NaCl/Pi containing 1% BSA Cells were incubated for ... observed, with no changes in the other isoforms of the kinase Tubulin Western blot images are included as loading controls Ó FEBS 2004 Differential role of PKC isoforms in APP processing (Eur J Biochem ... is consistent with data in the literature that indicate PKCe as the only protein kinase isoform involved in the signalling pathway downstream of muscarinic m3 receptors in SK-N-BE(C) neuroblastoma...
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Báo cáo khoa học: HIP/PAP, a C-type lectin overexpressed in hepatocellular carcinoma, binds the RIIa regulatory subunit of cAMP-dependent protein kinase and alters the cAMP-dependent protein kinase signalling ppt
... switch serines 73 and 138 and threonine 153 of the HIP/PAP protein for alanines Oligonucleotides cassettes containing the desired mutations were inserted into pcDNA3-HIP/PAPmyc-His as indicated ... HIP/PAP expression in hepatocellular carcinoma, we looked for proteins capable of interacting with this protein by screening a human HCC cDNA expression library in kgt11 using [32P]chimeric HIP/PAP ... overexpressed in primary liver cancer encodes for a C-type carbohydrate binding protein with lactose binding activity FEBS Lett 337, 114–118 Christa, L., Pauloin, A., Simon, M.T., Stinnakre, M.G., Fontaine,...
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Báo cáo Y học: Extra terminal residues have a profound effect on the folding and solubility of a Plasmodium falciparum sexual stage-specific protein over-expressed in Escherichia coli pptx
... differing protein solubilities were inherent in the constructs and could not be modulated by varying the ambient growth conditions DISCUSSION The routine production of recombinant proteins in soluble ... repressor protein Ara C Aldehyde dehydrogenase C-terminal tail Increased stability C-terminal tail Increased stability Reference [4] [5] C-terminal tail Increased stability [11] C-terminal tail Increased ... MBP-Pfg27–70K fusion protein marked by an arrow according to manufacturer’s instructions All constructs showed equivalent levels of protein induction but the resulting fusion protein had varying solubilities...
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Báo cáo khoa học: "LOGICAL FORMS IN THE CORE LANGUAGE ENGINE" docx
... satisfying the body of the quantification, so this reading involves five computers and five buying events The operator set is introduced during scoping since collective/distributive distinctionsmlike ... Paperclip(p) ) ) ) In reasoning about kinds, the simplest approach possible would be to have a rule of inference stating that if a "kind individual" has a certain property, then all "real world" individuals ... qterm(every, sing, v, Art(v)))) Quantifier scoping determines the scopes of quantifiers and operators, generating scoped logical forms in a preference order The ordering is determined by a set...
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Báo cáo Y học: Secretion of egg envelope protein ZPC after C-terminal proteolytic processing in quail granulosa cells doc
... band was also observed during insulin biosynthesis in pancreatic b cells [55], which is regarded as an intermediate of proinsulin to insulin conversion Because the intensity of the 94-kDa band ... indicate that BFA inhibits the secretion of ZPC by inhibiting the conversion of proZPC to 35-kDa ZPC C-terminal sequence of 35-kDa ZPC In order to determine the C-terminal amino acid of 35-kDa ... C-terminal amino acid was determined as Phe by an automated C-terminal protein sequencer Effects of monensin and BFA on proteolytic processing of proZPC Next, we evaluated the effects of monensin...
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o'reilly - .net windows forms in a nutshell
... document window is active Windows Forms is able to manage MDI menus correctly for us, including automatically merging a child window’s menu into the main application window The details of menu merging ... need to define a Main method at all The Main function turns out to be trivial in the majority of applications because most interesting initialization takes place inside individual forms All that ... Chapter 3Forms, Apps, Containers Forms, Containers, and Applications Any interactive application must have at least one window through which to present its user interface In the Windows Forms framework,...
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differential forms in geometric calculus
... (2.9) can be calculated from (2.7) using (2.6) Instead of beginning with coordinate systems, the coordinate-free approach to vector manifolds in [5] begins by assuming the existence of a pseudoscalar ... attaching Stokes’ name to the theorem is brevity and custom His only role in originating the theorem was setting it as a problem in a Cambridge exam after learning about it in a letter from Kelvin ... for an intrinsic characterization of manifolds to replace the extrinsic characterization with coordinates This is not to say that coordinates are without interest It merely displaces coordinates...
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Báo cáo khoa học: Specific Ca2+-binding motif in the LH1 complex from photosynthetic bacterium Thermochromatium tepidum as revealed by optical spectroscopy and structural modeling pdf
... structural domain constituting the Ca2+ binding motif is found in a large number of Ca2+-binding proteins, and is also known as the EF hand [16] Proteins containing the EF hand are divided into two ... orange, amino acids of LH1 polypeptides; red, His coordinating to BChl a in LH1 of Tch tepidum; purple, His coordinating to BChl a in the template; yellow, hydrogen atoms coordinating to BChl ... 273 to 323 K The increase in spectral shift or bandwidth in response to the increase in temperature may indicate the involvement of more thermally populated excitonic states in the Qy-state manifold...
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Báo cáo khoa học: Molecular identification and expression study of differentially regulated genes in the Pacific oyster Crassostrea gigas in response to pesticide exposure doc
... b-1,3-Glucan binding protein 3e-6 Putative senescence-associated protein 5e-46 Cavortin 1e-5 Cytoskeleton Tubulin alpha chain 4e-17 Tubulin alpha chain 5e-20 Tubulin beta 1e-50 Actin cytoplasmic ... b-1,3-Glucan binding protein 3e-6 Procathepsin L 1e-12 Meningioma expressed antigen 7e-19 Lipoprotein receptor related protein 2e-24 Fucolectin-1 6e-4 Cytoskeleton Tubulin alpha chain 4e-40 Tubulin beta ... monitoring can be determined Other interesting genes that were identified from our libraries are the lysosomal associated protein and procathepsin L, a protein protease Both are involved in FEBS...
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lindell i. differential forms in electromagnetics (wiley,2004)
... to work in 1854 The foreword bears the date 29 August 1861 Grassmann had it printed on his own expense in 300 copies by the printer Enslin in Berlin in 1862 [29] In its preface he complained the ... Grassmanns way of thinking and eventually developed into the algebra carrying his name In the beginning of the 19th century, the classical analysis based on Cartesian coordinates appeared cumbersome ... move forward ISMO V LINDELL Koivuniemi, Finland January 2004 Differential Forms in Electromagnetics Differential Forms in Electromagnetics Ismo V Lindell Copyright 2004 Institute of Electrical...
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windows forms in action 2nd edition
... Windows Forms in Action Windows Forms in Action Second Edition of Windows Forms Programming with C# ERIK BROWN MANNING Greenwich (74° w long.) For online information and ordering of this ... 22.1 Binding lists 638 The IBindingList interface 638 22.2 Editable objects ✦ Creating a binding list 640 643 The IEditableObject interface object 644 22.3 Simple data binding 644 ✦ Creating an ... Understanding automatic completion 341 Using auto-completion in a combo box 344 12.4 Item formatting 345 The formatting interfaces 345 ✦ Implementing an IFormattable interface 346 ✦ Using formatting in...
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C# Auto Fill Forms ppt
... SetText(string attribute, string attName, string value) { // Get a collection of all the tags with name "input"; HtmlElementCollection tagsCollection = webBrowser1.Document.GetElementsByTagName("input"); ... values in Combo boxes and works like a charm Thanks dude Here is the code public void SetComboText(string attribute, string attName, string value) { // Get a collection of all the tags with name “input”; ... values in Combo boxes and works like a charm Thanks dude Here is the code public void SetComboText(string attribute, string attName, string value) { // Get a collection of all the tags with name “input”;...
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Báo cáo sinh học: " Transmission of human hepatitis C virus from patients in secondary cells for long term culture" pot
... protein pellet was solubilized into 200– 500 µl of 1% SDS by incubating at 55°C for 10 minutes Any remaining insoluble subcellular particles were removed by centrifugation at 14000 × g for 10 minutes ... analysis of HCV proteins binding to IgG from patient sera Dot blot analysis of HCV proteins binding to IgG from patient sera HCV proteins were obtained from tissue culture fluid Protein preparations ... within an hour of blood drawing Repeat samples were obtained from 77 of the original patients in order to confirm our initial results Thirty-three of these 151 HCV-infected patients were co-infected...
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