cytokines chemokines and signalling
Báo cáo y học: "Human T-cell leukemia virus type I infects human lung epithelial cells and induces gene expression of cytokines, chemokines and cell adhesion molecules" ppt
... significance of aberrant production of inflammatory cytokines and chemokines in the lung has been given increasing attention and a variety of cytokines and chemokines are considered to play important ... the production of cytokines and chemokines and induced the surface expression of cell adhesion molecule http://www.retrovirology.com/content/5/1/86 Activation of NF-κB and AP-1, and viral promoter ... Figure of expression of cytokines, chemokines and cell adhesion molecule in A549 cells cocultured with MT-2 cells Induction Induction of expression of cytokines, chemokines and cell adhesion molecule...
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Báo cáo khoa học: Compartmentalized signalling: Ras proteins and signalling nanoclusters pdf
... Compartmentalized Ras signalling J Omerovic and I A Prior Fig Ras activation and effectors Ras is a molecular switch cycling between inactive GDP- and active GTP-bound conformations controlled by GEFs and GAPs ... now switching towards understanding context-dependent regulation of interactions and outputs within this large signalling web Ras trafficking and localization H-, K-, and N-Ras share almost complete ... FEBS J Omerovic and I A Prior Compartmentalized Ras signalling Fig Compartmentalization of Ras GEFs, GAPs and scaffolds Ras has been localized to and signals from both cell surface and endomembrane...
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Báo cáo y học: "Coexpression and interaction of CXCL10 and CD26 in mesenchymal cells by synergising inflammatory cytokines: CXCL8 and CXCL10 are discriminative markers for autoimmune arthropathie" doc
... such as astrocytes and keratinocytes [710] In addition, some Toll-like receptor ligands (e.g doublestranded RNA) stimulate the production of these CXCR3 ligands in leukocytes and fibroblasts [11,12] ... enzymes when cytokines act simultaneously CXCR3 ligands are nevertheless good substrates for CD26/DPP IV, which inactivates the CXCR3 ligands as chemoattractants [22] Cytokines and proteases, ... CXCL8, and synovial concentrations of both chemokines were compared in RA, PsA, AS and crystalinduced arthritis (CA) Materials and methods Reagents Recombinant human IL-1β, TNF-α, IFN-γ and CXCL10...
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Báo cáo y học: " Asthma families show transmission disequilibrium of gene variants in the vitamin D metabolism and signalling pathway" pot
... 25-OH-D3 (Figure 3) Data handling and statistical analysis Clinical data and genotypes were all transferred to a SQL 2000 database and checked for completeness, paternity, and Hardy-Weinberg equilibrium ... the tested candidate genes are associated with both allergy and vitamin D metabolites Statistical significance, however, was weak, and varied even with different analysis strategies and software ... analysis and wrote the report J.A did the clinical survey, C.B did the SNP analysis, M.B built serum and DNA bank and did the vitamin D assays together with E.A who supervised also laboratory work and...
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Báo cáo y học: "Ischemia as a possible effect of increased intraabdominal pressure on central nervous system cytokines, lactate and perfusion pressures" ppsx
... National and Kapodistrian University of Athens), conforms to our institutional standards and is under the appropriate license of the veterinary authorities and in adherence to National and European ... contributions AM, EA and DV designed the study; AM, EA, PV, GP and EB conducted the experiments PB and KT statistically analyzed the results AM and EA drafted the manuscript; AM, EA, EB and DV critically ... was maintained and boluses of 25 to 50 μg fentanyl and mg midazolam were administered according to needs Fluid infusion rate was standardized at ml/kg/h during pneumoperitoneum and was modified...
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Báo cáo y học: " Bronchoalveoloar lavage fluid cytokines and chemokines as markers and predictors for the outcome of interstitial lung disease in systemic sclerosis patients" pps
... autoantibodies, Th2 cytokines, growth factors, and several other cytokines/ chemokines [42,43] As detected with our first analysis including 17 different cytokines, profibrotic cytokines such as ... SSc-related ILD Conclusions High BALF cytokine and chemokine levels are associated with severe ILD in SSc and are associated with deterioration of ILD Cytokines and chemokines could have a role in the disease ... determined levels of cytokines and chemokines in BAL fluids (BALF) in an early SSc cohort Furthermore, we analyzed controls with ILD due to other diseases to identify key cytokines specifically...
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Báo cáo y học: "Terameprocol, a methylated derivative of nordihydroguaiaretic acid, inhibits production of prostaglandins and several key inflammatory cytokines and chemokines" ppsx
... of cytokines, chemokines, and inflammatory lipids from activated macrophages Our results show that TMP can inhibit production of both prostaglandins and several key inflammatory cytokines and chemokines ... production of a number of cytokines and chemokines (Figs 3C and 4C) For purposes of discussion we have divided these into two groups One group of cytokines and chemokines was induced to high levels, ... simultaneously detects 21 cytokines and/ or growth factors and 15 chemokines The array also contains antibodies for tissue inhibitor of metalloprotease-1 (TIMP-1) and -2 (TIMP-2) and for soluble TNF...
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Tài liệu Báo cáo khoa học: The plasminogen activator inhibitor 2 transcript is destabilized via a multi-component 3¢ UTR localized adenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenes pdf
... SJS133 and SJS134 as the external primers and the constructs pTETGLOPAI)2 and pTETGLOARE II-MUT as the templates Mutation of the ARE I-AUUUA and ARE III-AUUUA sequences used primers SJS172 and SJS173, ... SJS173, and SJS174 and SJS175, respectively Mutation of the atypical AU-rich sequence used primers SJS259 and SJS260, and the creation of the ARE II ⁄ ARE IV double mutant used primers SJS261 and ... pCMVglo-PAI-2 3¢ UTR and pCMV-glo-PAI-2 3¢ UTR-ARE MUT [48] with primers SJS133 and SJS134, and cloned into the BglII site in the b-globin 3¢ UTR in pTETGLO, to generate pTETGLOPAI)2 and pTETGLOARE...
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Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf
... (A, lanes and 5; B, lanes and 5) or 10 lM S-4o9cDH-RA (A, lanes and 6; B, lanes and 6), followed by incubation with trypsin or buffer only as indicated (for details, see Materials and methods) ... official nomenclature [10]) There are different RAR and RXR subtypes (a, b and c), and each subtype exists in multiple isoforms [11] at-RA binds to the ligand-binding domain of RARs, which induces heterodimer ... RARa and RXRb (A), RARa and RXRb (B), RXRa (C) or RXRb (D) Cells were treated with S-4o9cDH-RA at the indicated concentrations at-RA (100 nM) was used as a positive control in (A) and (B), and...
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Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx
... regulation of RGS1 and RGS2 expression Results RGS1 and RGS2 mRNA expression is regulated by LP and LPS BMDM were stimulated with FSL-1, a ligand for the TLR2 ⁄ heteromer, and LPS, a ligand for TLR4 ... by LP and LPS Thus, these results demonstrate that both BMDM and J774 cells express RGS1 and RGS2 mRNA and modulate expression after stimulation with LP and LPS Expression patterns of RGS1 and ... addition, RGS1 and RGS10, but none of the other listed RGS mRNAs, were also found to be modulated Stimulation with Pam2C-SK4 (TLR2 ⁄ and TLR2 ⁄ ligand) and PamOct2C(VPGVG)4VPGKG (TLR2 ⁄ ligand) showed...
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
... treatment of pea seedlings were cold (A and B), salinity (C and D), wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (N and O) Panel M is the control for ABA responsive ... Figure 7A, D, I and L are diamidino-2phenylindole hydrochloride (DAPI) stained cells showing the nucleus, and Fig 7C, F, K and N are the merged images of A and B, D and E, I and J, and L and M, respectively ... canonical EF hand The amino acid D at position 1, of EF1 is replaced by amino acid A (Fig 1B) The EF1 and EF2 are 22 amino acids apart, whereas EF2 and EF3, and EF3 and EF4 are 25 and 32 amino...
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Tài liệu Báo cáo khoa học: Platelet factor 4 disrupts the intracellular signalling cascade induced by vascular endothelial growth factor by both KDR dependent and independent mechanisms ppt
... VEGF- and FGF2-induced MAP kinase phosphorylation [17], PF-4 may act on a target common to the FGF2 and VEGF signalling pathways Thus, PKC does not seem to be a good candidate Raf1 is a key signalling ... obtained ` through local maternity units (Lariboisiere Hospital and Saint Isabelle Clinic) under approval, and with appropriate understanding and consent of the subjects DNA synthesis HUVEC were seeded ... induced by VEGF165 and VEGF121 was maximal between 10 and 15 of stimulation and decreased thereafter (Fig 4C,E) PF-4 strongly decreased ERK phosphorylation, to only 34% (VEGF165) and 22% (VEGF121)...
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Tài liệu Báo cáo khoa học: Synergistic activation of signalling to extracellular signal-regulated kinases 1 and 2 by epidermal growth factor and 4b-phorbol 12-myristate 13-acetate pptx
... concentration after stimulation with EGF and PMA together on the one hand, and the sum of the ERK-PP concentrations after stimulation with EGF and PMA separately on the other hand, was not constant, but increased ... NaCl/Pi and washed Fig Biphasic ERK-PP time profile induced by EGF or PMA alone and synergistic ERK phosphorylation induced by EGF and PMA together Cells were serum-starved for three days and subsequently ... half-maximum ERK phosphorylation Fig The complex structure of the signalling network to ERK Depicted are the MAPK and PKC signalling modules (blue and yellow boxes, respectively) Activated EGFR signals...
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Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc
... anti-a, -b, -c, -d, -e, and -f for immunoblotting were obtained from Gibco (Grand Island, NY, USA) Rabbit anti-PKCf for immunoprecipitation and activity determination, and the antiPKCf blocking ... rapidly aspirating the medium and immersing the dishes in liquid N2 Glycolysis and glycogen synthesis Materials and methods Materials Enzymes, M199 medium, collagenase A and the transfection agent ... fluoride, 20 mM 2-mercaptoethanol) and centrifuged at 100 000 g for 30 Supernatant and membrane fraction were diluted with lysis buffer (without sucrose and EGTA) and 8–14 lg of protein from each...
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Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx
... via the upregulation of SLex/a-1,3 FucT-VII, and its effects are mainly mediated by the PI-3K/PKB signalling pathway Materials and methods The H7721 and human umbilical vein endothelial cell (HUVEC) ... obtained after 2–3 weeks and re-cloned by serial dilution The stable cells transfected with sense and antisense PKB were named S-PKB/H7721 and AS-PKB/H7721 Cell culture and treatment Cells were ... Expression of PKB protein and activity in differently treated and transfected cells (A) Expression of PKB protein and b-actin (loading control) in differently treated and transfected cells (Western...
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Tài liệu Báo cáo Y học: The Fc receptor c-chain is necessary and sufficient to initiate signalling through glycoprotein VI in transfected cells by the snake C-type lectin, convulxin ppt
... and signalling features with other FcRs [1,3,17] The similarity between GPVI and different FcRs provides a starting point in the study of the structure–function relationship of GPVI and the signalling ... related PIR-A and Fca-receptor [5,9] and suggests that, in cell lines, the FcR c-chain is acting as a signalling and stabilizing subunit for some receptors, such as FcaR, but only as a signalling ... detectable expression of FcR c-chain ([16] and Fig 4) Mock-transfected cells (K562/pRc) did not express GPVI as detected by flow cytometry and ligand blotting (Fig 3A and Fig 4, respectively), whereas...
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Báo cáo khoa học: WFIKKN1 and WFIKKN2 bind growth factors TGFb1, BMP2 and BMP4 but do not inhibit their signalling activity docx
... and lM) with TGFb1; (D) WFIKKN2 (250 AND 500 nM, and 1, and lM) with TGFb1; (E) WFIKKN1 (400 and 750 nM, and lM) with BMP2; (F) WFIKKN2 (50, 100, 250 and 500 nM) with BMP2; (G) WFIKKN1 (500 and ... 750 nM, and 1.5 lM) with BMP3; (H) WFIKKN2 (100, 250 and 500 nM, and lM) with BMP3; (I) WFIKKN1 (250, 500 and 750 nM, 1, 1.25, 1.5 and lM) with BMP8b; (J) WFIKKN2 (250, 500 and 750 nM, and 1.5 ... inhibition being achieved by nm WFIKKN1 and by nm WFIKKN2 Similarly, WFIKKN1 and WFIKKN2 inhibited the activity of GDF11; 50 nm WFIKKN1 and WFIKKN2 caused 80% and 90% inhibition, respectively (data...
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Báo cáo khoa học: The SWI⁄SNF protein BAF60b is ubiquitinated through a signalling process involving Rac GTPase and the RING finger protein Unkempt doc
... interactions and functions of modified proteins [15,16] 1454 We report here on the occurrence of BAF60 ubiquitination We have identified and characterized a signalling process involving Rac GTPase and a ... digestion, Klenow extremities fill-in and re-ligation Human cDNAs of WTRac1 and WTCdc42, dominant negative mutant Rac1N17 and constitutively activated forms Rac1L61, Cdc42L61 and RhoAL63, cloned in pRK5-myc ... previously described drosophila protein, d-Unkempt [17], and with Unkempt-like sequences from human and mouse origin (accession UniProtKB ⁄ TrEMBL Q9H9P5 and Q6RUT6, respectively) Northern blot analysis...
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Báo cáo khoa học: Crosstalk between Src and major vault protein in epidermal growth factor-dependent cell signalling docx
... locations where they bind to regulatory and ⁄ or substrate proteins and are integrated into cell signalling pathways and cell cycle events [23] The UD, Src homology and Src homology (SH2) domains in ... mitosis and possibly during G1 ⁄ S transition Identification of Src binding proteins has led to a better understanding of Src regulation and has provided clues about the function of Src in normal and ... proteins that regulate Src activity and integrate Src into important signalling pathways and cell cycle events The aim of the present study was to identify and characterize Src interacting proteins...
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Báo cáo khoa học: Molecular basis of cytokine signalling – theme and variations docx
... during signalling [5] Thus, it seems that there exist a variety of intermediate receptor states between pure ‘horizontal’ and pure ‘vertical’ signalling This simple and elegant horizontal signalling ... effects, where ligand affinity is increased by binding simultaneously to two receptor chains The formation of heterodimeric ligands and ⁄ or multiple receptor chains might allow specific signalling modes, ... been determined and therefore we not know in molecular detail how they function The binding of two trigger and two driver chains to a dimeric ligand has profound consequences for BMP signalling Multiple...
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