cytokine induced igf 0resistance in vitro—mechanisms of action
báo cáo hóa học:" In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA" doc
... have definite effects on NO production in the joints These inhibitory effects of HA on cell apoptosis may play a role in its mechanism of action in chondroprotection [38] Our findings indicated ... min, and placed on ice until the addition of the remaining reaction components The reaction was incubated at 42°C for 90 min, and terminated by heat inactivation at 70°C for 15 Measurement of ... level of GAG appearing in the medium of explants, cell cultures, and papain-digested cells was determined using the dimethylmethylene blue (DMMB) assay for sulfated glycosaminoglycan 10 using chondroitin...
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Báo cáo y học: "RANKL increases the level of Mcl-1 in osteoclasts and reduces bisphosphonate-induced osteoclast apoptosis in vitro" pps
... Further insight into mechanism of action of clodronate: inhibition of mitochondrial ADP/ATP translocase by a nonhydrolyzable, adenine-containing metabolite Mol Pharmacol 2002, 61:1255-1262 26 Roelofs ... effects of BPs may account for the apparent lack of effectiveness of BPs (particularly the less potent BPs such as CLO and ALN) in preventing local, inflammation -induced bone loss in RA 11 Competing ... demonstrating that ALN inhibits protein prenylation in osteoclasts [2,22] Incubation of osteoclasts with 100 M ALN in the presence of 100 ng/mL RANKL for 48 hours did not prevent the accumulation of...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... Aggregates were incubated with 50 μL of the double labelling staining solution for 10 minutes at room temperature After staining, aggregates were washed five times with 100 μL of binding buffer, ... defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained regeneration of hyaline cartilage in vivo ... untransduced recombinant human protein controls were maintained, which were cultured in the presence of 10 ng/mL TGFβ1 protein, or 25 ng/mL BMP-2, or 25 ng/mL BMP-4 (all R&D Systems, Minneapolis, MN,...
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Báo cáo y học: "Anti-inflammatory effects of ciprofloxacin in S. aureus Newman induced nasal inflammation in vitro" potx
... J Interferon Cytokine Res 1999, 19:1031-1035 Lennard CM, Mann EA, Sun LL, Chang AS, Bolger WE: Interleukin1 beta, interleukin-5, interleukin-6, interleukin-8, and tumor necrosis factor-alpha in ... attributed to inhibition of the activation of NF-κB Miyanohara et al have evaluated the effects of clarithromycin on the H influenzae endotoxin induced expression of IL1β and ICAM-1 in HNECs and ... Newman and ciprofloxacin In a previous series of experiments we had already quantified Eotaxin which is a chemoattractant for eosinophils in Ciprofloxacin belongs to the fluoroquinolones and displays...
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In vivo and in vitro studies of th17 response to specific immunotherapy in house dust mite induced allergic rhinitis patients
... between in vivo and in vitro findings, confirming that not only Th2, but also Th17 cells play an important role in the context of allergic inflammation IL10 producing CD4+ T cells showed a distinct ... producing Treg cells can suppress Th17 response during inflammation [24] These findings suggest the induction of IL10 in T cells during chronic allergic reactions (e.g., SIT-untreated AR) could not effectively ... markers in each study group tested in vitro 3.2 In vivo Study 3.3.1 Th17 specific gene mRNA/protein expression Following a 3-day in vitro stimulation, there were mRNA/protein levels of Th17 associated...
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Analysis, sequencing and in vitro expression of PCR products
... also protein fragments for mapping of interaction domains The use of in vitro expression approaches to analyze protein products is likely to gain momentum for the analysis of known proteins as well ... associated with DNA sequencing including robotic automation of PCR set-ups, template purification, sequencing reactions and comb loading, all of which will serve to simplify sequencing of PCR products Primers ... functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction studies could be performed using this technique where not only full-length version of proteins could be...
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Tài liệu Women''''s attitudes towards mechanisms of action of family planning methods: survey in primary health centres in Pamplona, Spain doc
... in inconsistent responses We found inconsistencies in 12.2% of the sample, indicating the difficulties in communicating the information about the mechanism of action To assess the influence of ... would consider using and would stop or continue using In addition, hypothetical choices were examined in terms of a non-specific "method of family planning" and not in terms of actual methods ... cross-sectional survey in a sample of women in reproductive age (18–49) from ten Primary Care Health Centres in various areas of Pamplona, Spain Women attend this kind of centre for obtaining primary care...
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Báo cáo khoa học: Post-ischemic brain damage: the endocannabinoid system in the mechanisms of neuronal death ppt
... vitro [26] Experimental research in the field of ischemia was mainly prompted by observations indicating that CBs could attenuate glutamate -induced injury by inhibiting glutamate release via presynaptic ... detrimental effects of OGD in vitro by releasing glutamate in excess, indicating that the increase in 2-AG levels that was observed by these authors following OGD may protect dopaminergic neurons ... is worth noting that capsazepine has also been reported to prevent the neuroprotective effects of the agonist capsaicin in models of global ischemia [50] and ouabain -induced toxicity in vivo [51]...
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Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... reaction by using 32P-labeling primer or template in the presence or absence of DNA-binding agents (DDIs and doxorubicin) The DNA bulge binding of both compounds was detected by CD and UV melting ... is formed To gain insight into the binding of drugs to bulge DNA, several bulgecontaining oligonucleotides were selected as binding hosts for DDI-1A and DDI-1B CD spectroscopy of DDI-1A (Fig ... two-base bulge in DNA via stacking of its helically oriented aromatic ring systems on the bulge-flanking base pairs that define the long sides of the triangular prism binding pocket, with its amino sugar...
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Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
... provide a possible model of drug interaction in GEO cells, which involves the complex interaction of proteins involved in cell cycle regulation To recapitulate, combining CDO with cytotoxic chemotherapy ... alone did not increase the extent of staining However, in cells that had been cocultured with CDO and a cytotoxic drug, staining was significantly increased, indicating the presence of senescence ... was investigated in GEO cells only, as inhibition of cell proliferation was not seen in the HCT116 cells At the end of the treatment schedules, the surviving fractions of GEO cells were plated in...
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In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies pptx
... challenges of extrapolating in vitro findings to in vivo evaluation of plant resources The chapters in this book are written by experts interested in exploring and making better use of plant biodiversity ... remaining larvae into individual labelled petri dishes and stain with a few drops of helminthological iodine 10 Add 50 μL of helminthological iodine to each well on the culture plate 11 Using ... to the methods involved in collecting, preparing and screening plants for bioactive properties for use in manipulating key ruminal fermentation pathways and against gastrointestinal pathogens...
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Báo cáo khoa học: Pathways involved in testicular germ cell apoptosis induced by H2O2 in vitro doc
... pathways of signal transduction in H2O2 induced testicular germ cell apoptosis (A) Dose-dependent increase in expression of p53 protein coinciding with (B) a marked rise in transcript levels in all ... a marginal increase in protein was observed (Fig 6C,D) and the significance of this increase in FasL expression is yet to be explored Following the binding of FasL with Fas, activation of caspase-8 ... mRNA and protein, indicating the involvement of the intrinsic pathway in the H2O2 -induced testicular germ cell apoptosis (Fig 6A,B) The ability of Bcl-2 (an anti-apoptotic protein) as an antioxidant...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... (Fig 3E) Interestingly, the inclusion of IKK2 inhibition by SC-514 with our experimentally determined rate constants in the original model resulted in a delay in simulated peak and damping of subsequent ... = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software measures the intensity of NF-jB in ... software was used according to the manufacturers instructions (Scheme 1) A E C Ihekwaba et al containing 100 lgặmL)1 ampicillin A colony of E coli in LB agar plates was inoculated into 50 mL of...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
... filter binding assay in TK8 buffer with nM I-AniI and varying concentrations of end-labeled pCOBLE or uniformly labeled AnCOB pre-RNA RNA binding reactions contained mM MgCl2 and DNA binding reactions ... stringent concentration of KCl, there was a correlation between inhibition of endonuclease activity and inhibition of splicing activity indicating that RNA binding can be monitored by measuring ... M)1Æmin)1 under similar conditions [38] to these We measured the rate constant for the binding of DNA to protein kinetically and obtained a value of at least half this in TK8 buffer containing...
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Báo cáo khoa học: Chick embryo anchored alkaline phosphatase and mineralization process in vitro Influence of Ca 2+ and nature of substrates doc
... facilitated the interpretation of IR spectra of mineral deposits Distinct mineralization steps were observed during the GPI-bALPinduced hydrolysis of b-GP At incubation times in the range 1.5–3 h, two ... presence of anchored GPI-bALP (Fig 1) The presence of HA in the mineral samples was confirmed by In uence of other phosphate substrates in the mineralization process To examine the effects of substrates ... of HA No mineral material was formed in media lacking GPI-bALP Mineral calcium phosphate identification by infrared spectra To identify the quality and the type of mineral formation obtained in...
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Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot
... This might explain their different substrate preferences A fuller understanding of quinone–protein interactions in this class of enzyme may be achieved by testing the effect of sequence modification ... by Fisher and Rich [20] for quinone binding sites Although speculative at this stage, this consensus sequence was found in various quinone-binding proteins including AOX [20,21] It should be mentioned ... quinone binding site would be expected to be close to iron binding amino acids, which is the case here ([6], Fig 4) This potential quinone-binding site appears to be conserved in PTOX sequences (including...
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in vitro release of ketoprofen from proprietary and extemporaneously manufactured gels
... Effect of molarity and pH on the diffusion profile of ketoprofen .85 Effect of temperature on the diffusion profile of ketoprofen 86 Effect of mass on the diffusion profile of ketoprofen ... variability in percutaneous absorption, the precision of dosing, the reservoir capacity of the skin, heterogeneity and inducibility of the skin in turnover and metabolism, inadequate definition of bioequivalence ... include extrusion of lamellar bodies, loss of nucleus and increasing amount of keratin in the stratum corneum (12) The origins of the cells of the epidermis lie in the basal lamina between the...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... cells producing TGF-β resulted in the induction of Treg in vivo and in vitro in a murine model [52] Moreover, the blockade of TGF-β reduces Treg induction by the fusion cell vaccine and enhances ... each vaccination [27,33] Autologous FCs were irradiated, suspended in 0.3 ml normal saline, and underwent up to nine times vaccinations via SC injection in the left inguinal area at 2-week intervals ... (obtained with following informed consent and approved by our institutional review board obtained) was isolated and maintained in TIL Media I medium with 10% heat-inactivated FCS, mM L-glutamine,...
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Báo cáo sinh học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strain" pot
... http://www.virologyj.com/content/4/1/94 information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral blood ... comparing two different MYXV strains (a wild-type strain (T1) and a cell-cultured attenuated vaccinal strain (SG33) [14]) we verified the stability of the viral tropism in vitro Findings Three bovine ... KOP-R P3 BT MDBK Figure Permissivity of bovine cell lines for myxoma virus Permissivity of bovine cell lines for myxoma virus Bovine cells were maintained in DMEM (KOP-R and BT) or MEM (MDBK)...
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Báo cáo sinh học: "In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pot
... after adding T cells), fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of apoptotic cells by anti-caspase Ab e, f: In situ end-labeling (ISEL); ... through binding of CCL2 to its corresponding chemokine receptor CCR2 on the T cells This was demonstrated by blocking of T-cell migration in presence of addition of excess chemokine CCL2 in the ... cells resulting in tumor growth inhibition (reviewed in [25] and [1]) In mouse systems, ex vivo transduction of chemokines into tumor cells has provided potent tumor vaccines inducing tumor rejection,...
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