cytochrome p450 side chain cleavage and cholesterol monitoring
Báo cáo khoa học: Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast pptx
... cholesteryl[1-14C]oleate Side chain cleavage activity was measured as described [18] Steroid and sterol analyses Steroids and sterols were extracted from yeast cells and analyzed by gas chromatography ... from adrenodoxin to cytochromes p450scc and p4501 1beta J Biol Chem 272, 4883–4888 56 Cao, P.R & Bernhardt, R (1999) Interaction of CYP11B1 (cytochrome P-45011 beta) with CYP11A1 (cytochrome P-450scc) ... of cholesterol side chain cleavage Indeed, it is apparent that the expression of these three proteins is differentially modulated in hormoneproducing tissues For example, the corpus luteum and...
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Báo cáo khoa học: Human liver mitochondrial cytochrome P450 2D6 – individual variations and implications in drug metabolism docx
... polymorphisms of cytochrome P450 2D6 (CYP2D6): clinical consequences, evolutionary aspects and functional diversity Pharmacogenomics J 5, 6–13 Zanger UM, Raimundo S & Eichelbaum M (2004) Cytochrome P450 ... et al Glue P & Clement RP (1999) Cytochrome P450 enzymes and drug metabolism – basic concepts and methods of assessment Cell Mol Neurobiol 19, 309–323 Addya S, Anandatheerthavarada HK, Biswas G, ... Constitutive and inducible cytochromes P450 in rat lung mitochondria: xenobiotic induction, relative abundance, and catalytic properties Toxicol Appl Pharmacol 156, 231–240 13 Robin MA, Anandatheerthavarada...
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Tài liệu Báo cáo khoa học: Models and mechanisms of O-O bond activation by cytochrome P450 A critical assessment of the potential role of multiple active intermediates in oxidative catalysis doc
... puried cytochrome P450scc FEBS Lett 115, 175177 Tuckey, R.C & Kamin, H (1982) The oxyferro complex of adrenal cytochrome P-450scc Eect of cholesterol and intermediates on its stability and optical ... oxidation-reduction potential of cytochrome P-450 Biochemistry 28, 20712077 62 Sono, M., Andersson, L.A & Dawson, J.H (1982) Sulfur donor ligand binding to ferric cytochrome P450cam and myoglobin Ultraviolet-visible ... transformation of free and N-substituted amine functions by cytochrome P450 as means of bioactivation and detoxication Drug Metab Rev 34, 451 477 128 Lewis, D.F.V (1998) The P450 catalytic cycle and oxygenation...
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Báo cáo khoa học: Inter-flavin electron transfer in cytochrome P450 reductase – effects of solvent and pH identify hidden complexity in mechanism potx
... interaction of NADPHcytochrome P450 oxidoreductase with cytochrome P450 and cytochrome c J Biol Chem 270, 2747527480 27 Shen AL & Kasper CB (1996) Role of Ser457 of NADPHcytochrome P450 oxidoreductase ... of human NADPHcytochrome P450 reductase Biochemistry 40, 19561963 20 Sevrioukova IF & Peterson JA (1995) Reaction of carbon monoxide and molecular oxygen with P450terp (CYP108) and P450BM-3 (CYP102) ... human cytochrome P450 reductase FEBS Journal 275 (2008) 45404557 ê 2008 The Authors Journal compilation ê 2008 FEBS 4541 Electron transfer in cytochrome P450 reductase S Brenner et al CPR [30]) and...
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Báo cáo khoa học: Kinetic and binding studies with purified recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase from Mycobacteriumsp. strain HE5 ppt
... mycobacterial P450mor system B Sielaff and J R Andreesen the encoding operon revealed also the gene encoding the specific reductase, which is required for activity of the P450mor system (B Sielaff & J R Andreesen, ... distinct from other purified bacterial P450 systems which either utilize a Fe2S2 ferredoxin-like P450cam [23] or belong to the microsomal type of P450s like P450BM3 [24] and are reduced by a diflavin reductase ... substrates to cytochromes P450 usually induces transition of the heme from the low-spin 1154 B Sielaff and J R Andreesen state to the high-spin state, which results in a shift of the heme Soret band,...
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Báo cáo Y học: Baboon cytochrome P450 17a-hydroxylase/17,20-lyase (CYP17) Characterization of the adrenal microsomal and cloned enzymes docx
... porcine and hamster CYP17 catalyse the D4 and D5 hydroxylase and lyase reactions, yielding both DHEA and A4 [14–16] The alignment of mammalian cytochromes P450 with bacterial cytochromes P450 has ... cytochrome P450 17a-hydroxylase J Clin Endocrin Metab 77, 98–102 Jefcoate, C.R (1986) Cytochrome P-450 enzymes in sterol biosynthesis and metabolism In Cytochrome P450: Structure, Mechanism and ... 424 nm and a minimum at 409 nm is characteristic of cytochrome b5 [20] The concentration of baboon adrenal cytochrome P450 was 0.55 nmolÆmg)1 microsomal protein and the concentration of cytochrome...
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Báo cáo khoa học: Kinetic deuterium isotope effects for 7-alkoxycoumarin O-dealkylation reactions catalyzed by human cytochromes P450 and in liver microsomes Rate-limiting C-H bond breaking in cytochrome P450 1A2 substrate oxidation pdf
... substrates and comparing the v versus S plots (Figs and 3, and Tables and 3) The patterns were very similar for 7-OMe and 7-OEt coumarin, with P450 1A2 showing high isotope effects (8–16) and P450 ... of cytochrome P450 enzymes Biochemistry 33, 2927–2937 12 Ortiz de Montellano PR & De Voss JJ (2005) Substrate oxidation by cytochrome P450 enzymes In Cytochrome P450: Structure, Mechanism, and ... system, and these results should apply to in vivo considerations The conclusions may apply to other P450 reactions, at least to those catalyzed by P450 1A2 Experimental procedures Chemicals 7-OMe and...
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Báo cáo khoa học: Cytochrome P450 metabolizing fatty acids in plants: characterization and physiological roles pptx
... oxygen atoms in the carbon chain is dependent on cytochrome P450s (P450s) The involvement of P450s in FA metabolism in plants was first described in the context of cutin and suberin studies [1,2] ... Durst F (1981) ¨ Induction and specificity of a (cytochrome P450) -dependent laurate in -chain- hydroxylase from higher plant microsomes Eur J Biochem 119, 651–655 Cytochrome P450 metabolizing fatty ... x-hydroxylation x-hydroxylation in -chain hydroxylation in -chain hydroxylation x-hydroxylation x-hydroxylation in -chain hydroxylation Epoxidation Epoxidation in -chain hydroxylation in -chain hydroxylation x-hydroxylation...
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Báo cáo khoa học: Expression and purification of orphan cytochrome P450 4X1 and oxidation of anandamide pdf
... al P450 4X1 is located on chromosome close to another orphan P450, P450 4Z1, and P450s 4A11, 4A22 and 4B1 (http://www.ncbi.nih.gov/) The subfamily 4F P450s are clustered on chromosome 19p13.1 P450 ... J, Holla VR & Falck JR (2005) Cytochrome P450 and the metabolism and bioactivation of arachidonic acid and eicosanoids In Cytochrome P450: Structure, Mechanism, and Biochemistry (Ortiz de Montellano ... compilation ª 2008 FEBS 3715 P450 4X1 and anandamide oxidation K Stark et al Guengerich FP (2005) Human cytochrome P450 enzymes In Cytochrome P450: Structure, Mechanism, and Biochemistry (Ortiz de...
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Báo cáo khoa học: Demethylation of radiolabelled dextromethorphan in rat microsomes and intact hepatocytes Kinetics and sensitivity to cytochrome P450 2D inhibitors pot
... Ioannides, C., ed (1996) Cytochromes P450 Metabolic and Toxicological Aspects CRC Press, Boca Raton Lin, J.H & Lu, A.Y (1998) Inhibition and induction of cytochrome P450 and the clinical implications ... hepatic cytochrome P450s and steroidogenic CYP17 to the N-demethylation of aminopyrine Xenobiotica 29, 187–193 45 Kennedy, S.W., Jones, S.P & Bastien, L.J (1995) Efficient analysis of cytochrome P4501 A ... Gomez-Lechon, M.J & Castell, J.V (1993) A microassay for measuring cytochrome P450IA1 and P450IIB1 activities in intact human and rat hepatocytes cultured on 96-well plates Anal Biochem 213, 29–33...
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Báo cáo y học: " Tumour necrosis factor-α stimulates dehydroepiandrosterone metabolism in human fibroblast-like synoviocytes: a role for nuclear factor-κB and activator protein-1 in the regulation of expression of cytochrome p450 enzyme 7b" doc
... × 10E-5 mol/l, as described in Materials and methods RNA was isolated and cDNA was made and used for reverse transcription with GAPDH and cytochrome p450 enzyme 7b (Cyp7b) specific primers The ... concentrations PD98059 and SB23580 were dissolved in methanol (MeOH) and dimethylsulfoxide (DMSO), respectively, and used as controls *P < 0.05 (Student's t-test) Cyp7b = cytochrome p450 enzyme 7b Cyp7b ... Netherlands), dissolved in dimethylsulfoxide or methanol and used as controls The proteasome inhibitor PSI and the JNK inhibitor SP600125 were purchased at Omnilabo (Breda, The Netherlands) and...
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báo cáo khoa học: "Sub-cellular internalization and organ specific oral delivery of PABA nanoparticles by side chain variation" pdf
... based on the length of the side chains and unsaturated moieties coupled during synthesis (Figure 1A) It is important to understand the self-assembled procedure and the size and shape of different ... (PABA) as skeletal moiety and self assembled with different acid side chains to produce a library of fluorescent organic PABA nano-particles having different shapes and sizes and determined their ... images showed that the shape and size of each self assembled benzamide structure differs based on the length of the acid side chain (Figure 2) The saturated acid side chains mainly form tubular...
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báo cáo khoa học: " Targeted isolation, sequence assembly and characterization of two white spruce (Picea glauca) BAC clones for terpenoid synthase and cytochrome P450 genes involved in conifer defence reveal insights into a conifer genome" pdf
... 3CAR (A) and CYP720B4(C) and comparison of 3CAR with the grand fir (Abies grandis) Gene structure of (LIM) and pinene synthase (PIN) genes (B) Gene structure of white spruce 3CAR (A) and CYP720B4 ... constitutive, and wound- and insect-induced expression, and cellular and subcellular localization in spruce and Douglas fir Plant Physiol 2007, 143:410-424 Liu JJ, Ekramoddoullah AK: Identification and ... (1,452 bp) and eight introns and includes transcribed 5'- and 3'-UTRs of 38 bp and 134 bp, respectively (Figure 2B) The intronic region covers 50% of the gene sequence between the start and stop...
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Tài liệu Service Level and Performance Monitoring pptx
... understand some performance data and analysis terms There are three essential concepts to understanding performance monitoring These are throughput, queues, and response time From these terms, and ... required, and so on They are able to collect data, interpret data, and forecast needs 4667-8 ch20.f.qc 5/15/00 2:08 PM Page 725 Chapter 20 ✦ Service Level and Performance Monitoring SLM and Windows ... SL tools and technology This is where Windows 2000 Server comes in While clustering and load balancing are included in Advanced Server and Datacenter Server, the performance and system monitoring...
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Tài liệu Báo cáo khoa học: Cytochrome P450 Cyp4x1 is a major P450 protein in mouse brain doc
... Microsomal cytochrome P450- mediated liver and brain anandamide metabolism Biochem Pharmacol 50, 677–686 22 Strobel HW, Thompson CM & Antonovic L (2001) Cytochromes P450 in brain: Function and significance ... RatBrain Cytochrome- P450 – reassessment of monooxygenase activities and cytochrome- P450 Levels Drug Metabolism Disposition 23, 651–654 24 Warner M & Gustafsson JA (1994) Effect of ethanol on cytochrome- P450 ... this P450, and how this relates to physiological function Experimental procedures Animals and tissue Human cardiovascular system and 12-lane multitissue northern blots, human aorta cDNA library and...
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Tài liệu Báo cáo khóa học: Point mutations associated with insecticide resistance in the Drosophila cytochrome P450 Cyp6a2 enable DDT metabolism doc
... contained house fly cytochrome b5 (1 nmol) [42], house fly cytochrome P450- reductase (1 nmol) [8], CHAPS (0.15%), dilauroylphosphatidyl choline (1 mgÆmL)1) and 100 pmoles of cytochrome P450 In the control ... the pCW vector and to Dr Rahmani for the facilities to analyze the DDT metabolites We also thank Dr R Feyereisen for the house fly cytochrome P450 reductase and cytochrome b5 cDNAs and for their ... Expression of house fly CYP6A1 and NADPH -cytochrome P450 reductase in Escherichia coli and reconstitution of an insecticidemetabolizing P450 system Biochemistry 33, 2171–2177 Andersen, J.F., Walding,...
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Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafficking pdf
... different conformation between the receptor activated by cleavage and shedding on the one hand, and the receptor activated by the ligand on the other hand This may be due to different post-translational ... proper folding, trafficking and ligand-mediated signaling but not for ligand binding [37,40] The complete loss in the constitutive activity of the Ó FEBS 2003 Role of cleavage and shedding in TSHR function ... molecules present at the cell surface To understand the role of cleavage and shedding, we constructed deletion mutants, starting at the most N- or C-terminal cleavage sites that we had previously mapped...
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Tài liệu Báo cáo Y học: Convulxin induces platelet shape change through myosin light chain kinase and Rho kinase ppt
... (3 lM at 37 °C for 30 min) and monitored in a Kontron SFM 25 spectrofluorimeter thermostatically regulated at 37 °C Excitation and emission wavelengths were 340 nM and 510 nM, respectively Intracellular ... measured the appearance of a 20-kDa band, indicating MLC phosphorylation consequent to the activation of the two pathways, Ca2+-dependent and Ca2+-independent, and determined the action exerted ... (63.0 ± 10.9 for HA 1077 and 66.33 ± 12.6 for Y-27632 vs 78.6 ± 11.4 mean grey value), equal to 19.8% and 15% reduction, respectively (Fig 3A) The identity of the 20 kDa band detected was confirmed...
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Báo cáo khoa học: Comparing the substrate specificities of cytochrome c biogenesis Systems I and II docx
... Soret and a-band absorbance maxima are at 415 and 550 nm, respectively, for wild-type cytochrome c550, and at around 419 and 555 nm for the AXXAH-containing variant The spectrum of the wild-type cytochrome ... C38AF and C38AR; c550 C35AC38A, C35AC38AF and C35AC38AR H thermophilus cytochrome c552 and its AXXCH, AXXAH and CXXAH variants were expressed from the plasmids pEST210, pEST211, pEST212 and pEST213, ... small amounts of a typical low-spin, b-type cytochrome (Fig 5) The Soret band is red shifted by nm and the a-band by nm compared with the wild-type cytochrome c550, as would be expected for noncovalently...
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Báo cáo khoa học: Complementation of coenzyme Q-deficient yeast by coenzyme Q analogues requires the isoprenoid side chain ppt
... respiratory chain Therefore, we first considered the possibility that the different side chains affected the way in which the ubiquinone analogues interacted with the mitochondrial respiratory chain ... min), decylQ (17 min) and decylQ2 (22 min) The area under each peak was integrated and compared with standards containing known amounts (0–200 pmol) of CoQ2 and decylQ CoQ2H2 and decylQH2 were quantitated ... a 10-carbon saturated side chain, failed to support growth on nonfermentable substrates once glucose was depleted (Fig 1A) This suggested that the structure of the side chain was important for...
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