cysteine serine or threonine as nucleophilic residues
Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc
... tumor invasion and metastasis [16–18] Serine proteinases such as plasminogen activators, plasmin and trypsin also contribute to expression of malignant phenotypes in tumor cells [19,20] Recently, ... OVISE cell lines suggested matriptase as a candidate for the IGFBP-rP1-processing enzyme Moreover, it has been reported that matriptase prefers basic P1, P3 and P4 residues to cleave substrates [33] ... major component and two-chain forms Processing of IGFBP-rP1 by matriptase complexed with its inhibitor HAI-1, in agreement with the past reports [18,30] Therefore, soluble matriptase released...
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Báo cáo y học: "Antibiotics or probiotics as preventive measures against ventilator-associated pneumonia: a literature review" docx
... prophylactic antibiotic or probiotic therapy This manuscript does not deal with oropharyngeal decontamination with chlorhexidine, which has the same principles as SOD Isolated interventions for the prevention ... We did not Results The rationale for antibiotics or probiotics as preventive measures against infections Critical illness-associated infections Critical illness-associated infections have been ... rates [12] Disturbance or loss of the anaerobic flora would lead to increased colonization and increased infection risk with facultative aerobic bacteria In this theory, it has been suggested that...
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Luận văn english coordinators and, but, or as cohesive devices an analysis or errors made by secondary students
... These examples can be corrected as follows: Although she was tired, she went to work Or She was tired, but she went to work As you know, I work very hard Or You know that I work very hard Because ... on its use for a particular purpose or object For the purpose of a discussion on error correction in foreign language teaching, this writer defines an error as an utterance, form, or structure ... AND, BUT, OR to connect sentences The expected answers of this part are or, but, or, and, or, but (or and), and, or, but (or and), and, or, and but, or, and Students make a total of 83 errors with...
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Tài liệu Báo cáo khoa học: Diversity and junction residues as hotspots of binding energy in an antibody neutralizing the dengue virus doc
... Fab4E11-H6 MalE-E3-H6 was attached to the sensorchip surface and Fab4E11-H6 was in the soluble phase for these experiments, which were performed with the Biacore instrument (Table 2) The association of ... 0.32 0.08 0.24 0.05 0.03 either residues of MalE-E3-H6 or neighboring residues of Fab4E11-H6, e.g H-Trp96 and H-Glu97 which were the most important residues for this interaction (Table 1) Kinetics ... Laboratory Manual, 2nd edn Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA Duplay P, Szmelcman S, Bedouelle H & Hofnung M (1987) Silent and functional changes in the periplasmic...
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Tài liệu Báo cáo khoa học: Novel aggregate formation of a frame-shift mutant protein of tissue-nonspecific alkaline phosphatase is ascribed to three cysteine residues in the C-terminal extension pdf
... gene was reported for the first time in hypophosphatasia patients by Orimo et al [15] and TNSALP (1559delT) is transmitted as a recessive trait TNSALP (1559delT) was originally referred to as TNSALP ... noteworthy that a 66 kDa form, but not an 80 kDa form was partitioned into the aqueous phase As the 66 kDa form of the wild type migrates to the Golgi complex and becomes the 80 kDa form as described ... before being released into the medium In support of this, this secretory form was found to be sensitive to PNGase F but resistant to Endo H (Fig 4B, lanes & 6) On the other hand, the GPI-anchor-less...
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Tài liệu Báo cáo Y học: Evidence that a eukaryotic-type serine/threonine protein kinase from Mycobacterium tuberculosis regulates morphological changes associated with cell division docx
... IDT, Coralville, IN, USA PCR was carried out for 30 cycles (denaturation, 95 °C for 30 s per cycle; annealing, 50 °C for 30 s per cycle; elongation, 68 °C for for first 10 cycles and then for the ... same way as pMAL-PknA except that IPTG induction was not required Kinase assay The ability of PknA or the K42N mutant, as a purified fusion protein with MBP, to autophosphorylate and phosphorylate ... PknA equally, as phosphorylation of threonine was more than that of serine (Fig 1E, compare lanes and 4) This observation does not seem to be unusual as PknD, another Ser/Thr kinase from M tuberculosis,...
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Báo cáo khoa học: Cysteine residues exposed on protein surfaces are the dominant intramitochondrial thiol and may protect against oxidative damage docx
... (3000 g for at °C) giving the cell lysate as the supernatant The cell lysate was further centrifuged (10 000 g for 10 at °C) to generate the mitochondrial fraction as the pellet, which was washed ... concentration was measured by the bicinchoninic acid assay using BSA as standard [48] Pre-treatment of tissue or mitochondrial fractions with 50 mm N-ethylmaleimide for 10 prior Protein thiols ... before blocking the membrane and repeating the immunoblotting procedure with a different antibody Other assays Complex I activity was assessed as the NADH:HAR activity measured by the decrease...
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Báo cáo khoa học: Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity docx
... which Asp367 has been replaced with alanine or asparagine exhibit a 300- and 600-fold lower activity, respectively, emphasizing the important role of the Asp367 residue as an active-site base These ... lm for the wild-type and Ser127Ala mutant proteins or 12.5 lm of the Ser16Ala and Ser16AlaSer127Ala mutant proteins) Chorismate formation was monitored at 275 nm under anaerobic conditions For ... bifunctional chorismate synthase ⁄ flavin reductase from Neurospora crassa J Biol Chem 270, 20447–20452 18 Bornemann S, Lowe DJ & Thorneley RNF (1996) The transient kinetics of Escherichia coli chorismate...
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Báo cáo khoa học: Structure of Streptococcus agalactiae serine⁄threonine phosphatase The subdomain conformation is coupled to the binding of a third metal ion pptx
... from the flap domain [9] There are homologous residues to Asp118 and Asp191 in HsSTP (Asp146 and Asp239) and, of course, in SaSTP (Asp118 and Asp192) Our SaSTP crystal structure provides clear evidence ... D; Fig 2A) M3 was also modeled as a Mg2+ ion, based on the same principles as for M1 and M2 (see A Fig (A) Stereoimage of the active site of SaSTP monomer C Residues are shown as combination ... atoms from the model Before map calculation, the stripped model was refined for 20 cycles with REFMAC5 Coordinations for the metal ions and for Asn160 are indicated by dashed lines (B) Superimposition...
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Báo cáo khoa học: Leishmania donovani methionine adenosyltransferase Role of cysteine residues in the recombinant enzyme pptx
... cuvettes for 12 for the fluorescence signal to reach a constant value All fluorescence data were corrected when necessary for dilution and for fluorescence background of the refolding buffer used MAT assay ... Laboratory Manual, 2nd edn Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 18 Southern, E.M (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis ... FEBS 2003 Cysteine residues in leishmanial MAT (Eur J Biochem 270) 29 Long-term exposure of African trypanosomes to the irreversible ornithine decarboxylase inhibitor a-difluoromethylornithine...
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Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt
... GalNAc-T9; rppGaNTase-T9, rat ppGaNTase-T9 combination with identification of free cysteine residues (defined as cysteine residues not involved in the formation of a disulfide bond), by cysteine- specific ... was determined as described below The activity levels were corrected for enzyme protein concentration in the medium Assay for GalNAc-transferase activity (PD-10 assay) The enzyme activity was ... When serine was substituted for Cys214, the increase in Km was only slight ( 1.2-fold that of P-DN42) Fig Enzymatic activity of cysteine- to -serine mutant GalNAc-T1 enzymes Enzyme activity measurements...
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Báo cáo khoa học: Ligand binding and antigenic properties of a human neonatal Fc receptor with mutation of two unpaired cysteine residues docx
... FcRn-binding interface or surrounding residues Furthermore, any protein might be given an increased half-life by fusion to a normal or modified Fc region, as well as to albumin or to an albuminbinding ... soluble receptor for HC to be utilized for immunization, purification and selection of antibodies or other binders The HC preparation used for immunization was the same that was utilized for refolding, ... gel) (Bio-Rad Laboratories, Hercules, CA, USA) and then blotted onto a poly(vinylidene fluoride) membrane (Millipore Corporation, Bedford, MA, USA) in Tris ⁄ glycine buffer at 100 V for 1.5 h The...
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Báo cáo khoa học: Importance of tyrosine residues of Bacillus stearothermophilus serine hydroxymethyltransferase in cofactor binding and L-allo-Thr cleavage Crystal structure and biochemical studies pot
... and Tyr residues have been invoked as Ca proton abstractors in several PLPdependent enzymes, such as aspartate aminotransferase [11,12], 5-aminolaevulinate synthase [13] and alanine racemase [14] ... sodium cyanoborohydride (NaCNBH3) None The enzyme (1 mgÆmL)1) was incubated with 0.1 m NaOH for The PLP content was determined by measuring the absorbance at 388 nm assuming a molar absorption coefficient ... properties of threonine or allo -threonine aldolase from rat liver Biochim Biophys Acta 85, 117–131 Chen MS & Schirch LV (1973) Serine transhydroxymethylase A kinetic study of the synthesis of serine...
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Báo cáo khoa học: EmbR, a regulatory protein with ATPase activity, is a substrate of multiple serine⁄threonine kinases and phosphatase in Mycobacterium tuberculosis doc
... autoradiography For resin-bound kinase assays, purified EmbR was phosphorylated by PknH in the kinase buffer, as described previously [20] The counts associated with resin-bound EmbR are a measure ... dephosphorylation assays The prephosphorylated substrates for dephosphorylation assays were prepared using resin-based phosphorylation reactions in the presence of [32P]ATP[cP] In-solution phosphatase ... GTP hydrolysis by EmbR The release of Pi was measured, using the malachite green method, at various time points The Pi release was assayed when ATP or GTP was used as a substrate of EmbR Each time...
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Báo cáo khoa học: Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates doc
... gel was exposed, scanned and phosphorylation intensity was evaluated with aida 2.11 Protein phosphatase assay Protein phosphatase activity was measured using a serine ⁄ threonine phosphatase assay ... phosphorylated Two different modes for this initial phosphorylation have been proposed [26] First, a kinase-dependent phosphorylation with a nucleoside triphosphate as phosphoryl group donor, or ... The phosphatase activity of the purified PhpP was measured using a serine ⁄ threonine phosphatase assay system (Promega) Figure 2A shows that PhpP has the significant protein phosphatase activity...
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Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc
... electrophoresis, quantified and sequenced before use as probes for hybridization Northern blot Total RNA isolation from mouse tissues was performed by guanidinium thiocyanate ⁄ phenol ⁄ chloroform ... recognized as putative endoplasmatic reticulum membrane retention signals [37] The highest psort scores for subcellular localization based on amino acid composition correspond to cytoplasm and nucleus, ... phylogenetic analyses, originally found to be expressed mainly in testis [28], has been shown to be associated with hepatic disorders [29,30] DAP-kinases are serine ⁄ threonine kinases involved in...
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TEFL/TESL: TEACHING ENGLISH AS A FOREIGN OR SECOND LANGUAGE pot
... kinds of stories they liked as children, or the fascination of ghost stories and fairy stories Moreover, some students will probably have some stories they would like to tell or favorite characters ... context Ask your students to listen for one or two specific words, play a tape recording of a short passage (two to three minutes at most), and ask for guesses about the meaning of the words Have ... to speaking or listening The central text for each lesson is literary Passages are selected from authors such as Mark Twain, George Orwell, Charles Dickens, or modern writers such as Chinua Achebe...
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Báo cáo khoa học: Characterization of testis-specific serine–threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling doc
... indispensable, such as casein kinase 2a¢ (CK2a¢) [19]; whereas others were not e.g PAS domain serine threonine kinase (PASKIN) [20] We present evidence that TSSK3 is a genuine kinase that can be ... (B) Autoradiogram of TSSK3 kinase assay (using [32P]ATP[cP]) with test substrates: MBP, histone H1, casein; BSA, negative control Casein kinase II (CKII) was used as positive control for casein ... of kinases [22] that classifies TSSK3 as a member of a serine ⁄ threonine kinases family, containing a short sequence motif in the kinase subdomain VIB (DKCEN) diagnostic for Ser ⁄ Thr kinases...
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Báo cáo khoa học: Biosynthesis of platelet glycoprotein V expressed as a single subunit or in association with GPIb-IX doc
... treatment In contrast, the 80 kDa secreted molecule was insensitive to Endo-H (B) The cell associated 70 kDa form of GPV was resistant to neuraminidase, whereas the soluble 80 kDa form was reduced to ... presence or absence of GPIb–IX and in both cases an immature cell associated 70 kDa form was detected at early chase times In cells expressing only GPV, this form was still present at later chase ... Sylvette Chasserot for help in confocal microscopy studies at Plateforme d’Imagerie In vitro IFR des Neurosciences (Strasbourg) Catherine Strassel is supported by a grant for ARMESA ´ ´ (Association...
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What Germany Thinks or the War as Germans see it doc
... report of the explosion "According to the statement of the Governor of Warsaw it was caused by revolutionaries No proof of this was forthcoming, therefore it was ascribed to lightning, and as ... the British Ambassador's report, as well as some other phrases in the same, are evidently inspired by a certain bias They are intended to prove, by asserting that Austria-Hungary was prepared to ... our case, therefore, hate has sprung out of disappointed love England has become our mortal enemy, just as Russia is Austria's In a word, the two Central Powers are inspired by moral superiority...
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