cyclic gmp protein kinase and cyclic gmp affected cyclic amp phosphodiesterases

Tài liệu Báo cáo Y học: Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes pot

Ngày tải lên : 22/02/2014, 07:20

... AMP- activated protein kinase, and lack of activation by cyclic- AMP- dependent protein kinase, studied using a speciﬁc and sensitive peptide assay Eur J Biochem 186, 123–128 Marsin, A.S., Bertrand, L., Rider, ... L.A (1999) Dealing with energy demand: the AMP- activated protein kinase Trends Biochem Sci 24, 22–25 22 Hardie, D.G & Carling, D (1997) The AMP- activated protein kinase – fuel gauge of the mammalian ... becomes negative and, as a result, the AMP/ ATP ratio increases This leads to the activation of the AMP- activated protein kinase (AMPK), which functions as a metabolic master switch and inhibits anabolic...

9
455
0

Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf

Ngày tải lên : 16/03/2014, 12:20

... embryonic zebraﬁsh kinases and allow us to further characterize CNBP phosphorylation and to identify the candidate kinases CNBP is differentially phosphorylated in vitro by protein extracts prepared ... mobility controls: labelled Comp-CT alone and a completely annealed reaction Samples were taken at 0, 1, 3, 10, and 30 Single-stranded (ss) and double-stranded (ds) species are indicated (B) Bar ... cAMP-dependent protein kinase To identify kinases capable of phosphorylating CNBP, we carried out in vitro phosphorylation assays using embryonic extracts previously incubated with speciﬁc kinase...

13
500
0

Báo cáo khoa học: Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identiﬁcation of kinase substrates doc

Ngày tải lên : 16/03/2014, 18:20

... of both the wild-type and STPK loss-of-function mutant led to identiﬁcation of protein kinase target in prokaryotes 1244 Results and Discussion StkP is a protein Ser/Thr kinase capable of autophosphorylation ... autophosphorylation To characterize a putative protein kinase StkP, the stkP gene and its truncated form containing kinase domain were cloned in pET28b and expressed as Histagged proteins in E coli BL21(DE3) To ... (100 %) and compared with the activity of mutated enzyme StkPK42R (lane 2) and StkP in the presence 0.1 mM, mM and 10 mM of staurosporine (lanes 3, 4, and 5, respectively), a protein kinase inhibitor...

12
466
0

Báo cáo khoa học: HIP/PAP, a C-type lectin overexpressed in hepatocellular carcinoma, binds the RIIa regulatory subunit of cAMP-dependent protein kinase and alters the cAMP-dependent protein kinase signalling ppt

Ngày tải lên : 23/03/2014, 13:20

... polymerase and the kgt11 insert screening amplimer set (Clontech) were directly sequenced Materials and methods Cell culture and transfection Plasmid constructs The HIP/PAP(29–175) coding sequence ampliﬁed ... autoradiography of the wet gel Protein kinase assays Two independent clones of stably expressing HIP/PAP Chang cells (HIP and HIP 4) and two independent control clones (PC4 and PC8) stably transfected ... stably expressing HIP/PAP (HIP9 and HIP4 clones), and in two clones of Chang cells stably transfected with the empty vector as controls (PC4 and PC8 clones) Protein kinase activity assayed with...

9
310
0

Báo cáo Y học: Interaction between p21-activated protein kinase and Rac during differentiation of HL-60 human promyelocytic leukemia cell induced by all-trans-retinoic acid pdf

Ngày tải lên : 31/03/2014, 15:20

... column were pooled and employed to detect PAK and Rac by Western blot Protein was quantitated by the method of Bradford [25], using BSA Immunoprecipitation Protein samples of cytosol and plasma membranes ... contained proteins of human neutrophil cytosol (A) and plasma membrane (B) The cytosol (20 lg protein) and solubilized membrane (15 lg protein) were subjected to SDS/PAGE (10% gel), and then electrically ... at °C, and then protein A–agarose beads were added and gently stirred for h After washing the beads, the immunoprecipitates (150 lg protein) were loaded onto SDS/PAGE and then PAK (A) and Rac...

8
419
0

THE INFLUENCE OF THE KU80 CARBOXY-TERMINUS ON ACTIVATION OF THE DNA-DEPENDENT PROTEIN KINASE AND DNA REPAIR IS DEPENDENT ON THE STRUCTURE OF DNA COFACTORS Derek S.

Ngày tải lên : 24/08/2014, 09:33

... dependent protein kinase catalytic subunit PIKKs phosphatidylinositol-3 kinase- like protein kinases FAT FRAP, ATM, and TRRAP C-terminus Carboxy terminus dsDNA double-strand DNA ssDNA single-strand ... interactions with Ku and DNA in vivo and in vitro66 In order to better understand how protein/ protein and protein/ DNA interactions influence DNA-PK activation, we created and purified Ku80 C-terminal ... were collected and were individually assays for protein concentration and DNA-PK kinase activity using Bradford assays and Signatech DNAPK Kinase assay respectively Signatech DNA-PK Kinase assay...

104
281
0

Tài liệu Báo cáo khoa học: Steady-state and time-resolved ﬂuorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt

Ngày tải lên : 20/02/2014, 23:20

... by cAMP and DNA binding to CRP (Eur J Biochem 270) 1423 Won, Y.-S., Lee, T.-W., Park, S.-H & Lee, B.-J (2002) Stoichiometry and eect of the cyclic nucleotide binding to cyclic AMP receptor protein ... Interaction of cAMP receptor protein from Escherichia coli with cAMP and DNA studied by dynamic light scattering and timeresolved uorescence anisotropy methods J Protein Chem 20, 601610 31 Sứgaard-Andersen, ... Trp13 as a function of cAMP concentration F and F0 are the uorescence intensities of the protein in the presence and absence of the ligand, respectively The range of cAMP concentrations used...

11
494
0

Cyclic stretch enhances the expression of Toll-like Receptor 4 gene in cultured cardiomyocytes via p38 MAP kinase and NF-B pathway doc

Ngày tải lên : 10/08/2014, 05:21

... Results Cyclic stretch enhances toll-like receptor protein and mRNA expression in cultured cardiomyocytes To test the effect of cyclic stretch on the TLR4 expression, 10% and 20% of cyclic stretch ... TLR4 protein by cyclic stretch was largely mediated by p38 MAP kinase pathway because the specific and potent inhibitors of an upstream p38 kinase, SB203580 inhibited the induction of TLR4 protein ... before cyclic stretch completely inhibited the increase of TLR4 induced by cyclic stretch Addition of goat IgG 30 before cyclic stretch did not affect the protein expression of TLR4 induced by cyclic...

13
289
0

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

Ngày tải lên : 14/02/2014, 19:20

... fragments [the cAMP-dependent protein kinase regulatory subunit RII binding domain (RII), the central domain (CD)1-3 and the microtubulebinding domain (MBD)] and corresponding GST fusion proteins were ... residues, and provided evidence that these novel protein protein interaction core modules play a pivotal role in regulating dendrite growth and branching of cerebellar granule cells and hippocampal ... domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were generated by PCR and cloned into...

11
658
0

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

Ngày tải lên : 14/02/2014, 19:20

... their homology to the mammalian cAMP-dependent protein kinase A, cGMP-dependent protein kinase G and phospholipid-dependent protein kinase C [8] However, the AGC VIII kinases represent a plant-specific ... external stimuli and consist of three sequentially acting protein kinases: a MAPK kinase kinase, a MAPK kinase (MAPKK) and finally a MAPK [19] However, little is known about the function and composition ... PTI1-4 (A) In vitro kinase assay using recombinant proteins: HIS-OXI1 (OXI), kinase- deficient HISOXI1K45R (OXI1K45R) and HIS-PTI1-4 (PTI) Protein mixes were incubated in kinase buffer and [c-32P]-ATP...

11
700
0

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

Ngày tải lên : 18/02/2014, 16:20

... 26–34 47 Hedbacker K, Townley R & Carlson M (2004) Cyclic AMP- dependent protein kinase regulates the subcellular localization of Snf1-Sip1 protein kinase Mol Cell Biol 24, 1836–1843 48 Wiatrowski ... study both gene function and metabolism The protein translocation machineries of both the mitochondria and microsomes are highly conserved among mammals and yeast [24,25] As protein trafficking has ... full-length CYP1A1, + ⁄ 1A1 and + 331A1 showed significant levels of full-length CYP1A1 protein, reduced levels of + ⁄ 1A1 protein, and vastly reduced levels of + 33 ⁄ 1A1 protein (Fig 2A, first four...

16
650
0

Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf

Ngày tải lên : 18/02/2014, 16:20

... leupeptin and pepstatin, twice in kinase buffer, and used for in vitro kinase assays In vitro kinase assays Recombinant human protein kinase CK2 holoenzyme (0.5 U, corresponding to ng; New England ... loop between protein kinase CKII and Cdc37 promotes the activity of multiple protein kinases J Biol Chem 278, 2829–2836 Miyata Y & Nishida E (2004) CK2 controls multiple protein kinases by phosphorylating ... survival squad: an emerging role for protein kinase CK2 Trends Cell Biol 12, 226–230 Meggio F & Pinna LA (2003) One-thousand -and- one substrates of protein kinase CK2? FASEB J 17, 349–368 Allende...

14
553
0

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

Ngày tải lên : 19/02/2014, 18:20

... the second messenger cAMP [20], resulting in several intracellular effects, e.g activation of cAMP-dependent protein kinase (PKA) and exchange proteins directly activated by cAMP (Epacs), GEFs speciﬁc ... Ras-dependent mitogen-activated protein kinase activation by G protein- coupled receptors Convergence of Gi- and Gq-mediated pathways on calcium ⁄ calmodulin, Pyk2, and Src kinase J Biol Chem 272, 19125–19132 ... increase and show Ca2+ dependence of serotonininduced ERK1 ⁄ phosphorylation and a mandatory role of the Ca2+ ⁄ CaM-binding IQ domain in RasGRF1-stimulated ERK1 ⁄ phosphorylation Thus, both cAMP and...

13
730
0

Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc

Ngày tải lên : 20/02/2014, 02:21

... Determination of protein kinase C (PKC) activity in crude extracts (pmolÆmin-1Æmg-1 of protein) and PKCf immunoprecipitates (pmolÆmin-1Æmg-1 of lysate protein) Hepatocytes cultured for h and 48 h were ... ﬁbers in the propagation of hormone and growth factor signals The recent ﬁnding that focal adhesion kinase regulates protein kinase B, glycogen synthase kinase- 3 and glycogen synthase, in an insulindependent ... (PMA), insulin and ATP on the distribution of protein kinase C (PKC) isoforms Hepatocytes cultured for h and 48 h were incubated with 0.1 lM PMA, 10 nM insulin or 0.1 lM ATP for min, and subsequently...

12
592
0

Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx

Ngày tải lên : 21/02/2014, 00:20

... such as receptor protein tyrosine kinase/ growth factor receptor binding protein 2-son of sevenless protein/ Ras/Raf/mitogen activated protein kinase kinase/mitogen activated protein kinase pathway ... Fig Expression of PKB protein and activity in diﬀerently treated and transfected cells (A) Expression of PKB protein and b-actin (loading control) in diﬀerently treated and transfected cells ... the expression of PKB protein was elevated to 201% and 253% of the UnC and mock values, respectively, after insulin treatment The PKB protein was also increased to 233% and decreased to 55% of...

11
615
0

Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx

Ngày tải lên : 22/02/2014, 04:20

... of protein kinase inhibitors on the enhancement by TS of LPS/IFN-induced NO production Protein kinases such as PKA and PKC, and various factors such as tumor necrosis factor receptor (TRAF6) and ... Q.-Y., Hantash, F.M., Sanders, B.G & Kline, K (2001) Activation of extracellular signal-regulated kinase and c-Jun NH2-terminal kinase but not p38 mitogen-activated protein kinases is required for ... coexistence of a-T, TA and TN (Figs and 4), but not of the antioxidants BHA and AsA These results suggested that active oxygens and free radicals did not participate in the TS effect, and that the inhibitory...

6
494
0

Báo cáo khoa học: WD-repeat-propeller-FYVE protein, ProF, binds VAMP2 and protein kinase Cf pptx

Ngày tải lên : 07/03/2014, 10:20

... with both proteins, FlagVAMP2 and HA-PKCf These ﬁndings raised the question how ProF would affect the interaction between VAMP2 and PKCf In order to test this, we expressed Flag-VAMP2 and HA-PKCf ... interacts with VAMP2 and PKCf Fig Interactions of VAMP2 with ProF and PKCf (A) Flag-VAMP2 was transiently expressed in human embryonic kidney 293T cells The complex comprising Flag-VAMP2 and endogenous ... 2007 FEBS 1557 WD-FYVE protein interacts with VAMP2 and PKCf T Fritzius et al Endogenous VAMP2 interacts with ProF and PKCf Fig Interactions of endogenous VAMP2 with ProF and PKCf in brain (A)...

15
275
0

Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc

Ngày tải lên : 07/03/2014, 12:20

... the kinases calcium ⁄ calmodulin-dependent protein kinase (CaMKII), extracellular signal-regulated kinase ⁄ (ERK1 ⁄ 2), p38 mitogen-activated protein kinase (MAPK), protein kinase C and PI3 kinase ... (p65) 50 Fig ATP-mediated activation and nuclear translocation of extracellular signal-related kinase ⁄ (ERK1 ⁄ 2), p38 mitogen-activated protein kinase (MAPK) and nuclear factor jB (NF-jB) LXF-289 ... signal-regulated kinase and phosphoinositide 3 -kinase signaling pathways in somatic cell mitosis Mol Cell Biol 22, 7226–7241 Zarubin T & Han J (2005) Activation and signaling of the p38 MAP kinase pathway...

12
403
0

Báo cáo khoa học: Puriﬁcation and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf

Ngày tải lên : 07/03/2014, 15:20

... of the protein kinase inhibitor of cAMPdependent protein kinase J Biol Chem 272, 18169–18178 Gamm, D.M & Uhler, M.D (1995) Isoform-speciﬁc diﬀerences in the potencies of murine protein- kinase ... isoforms of the endogenous protein kinase inhibitor of cAMP-dependent protein kinase in mouse J Biol Chem 268, 10927–10931 Olsen, S.R & Uhler, M.D (1991) Inhibition of protein kinase- a by overexpression ... human PKIb; the bands at 1675, 1636 and 1625 cm)1 can be assigned to extended chain structures; the bands at 1683, 1669 and 1663 cm)1 are possibly assigned to turns or bends; and the band at 1647...

6
531
0

Báo cáo khoa học: The 3¢-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR ppt

Ngày tải lên : 08/03/2014, 08:20

... alone (lanes and 4), with lg bovine serum albumin (BSA) (lanes and 5), with lg protein of total extract from Swiss 3T3 cells (lanes and 6) After RNase T1 digestion (lanes 2, 3, and 6) samples were ... SDS-sample buffer and heating for 10 at 95 °C The samples were loaded on a 12.5% (w/v) SDS-polyacrylamide gel and separated overnight with 50 V at room temperature Finally, the gel was dried and ... recombinant Hu proteins To acquire recombinant GST-HuD fusion protein we ampliﬁed a cDNA encoding residues 2–373 of HuD by PCR using clone kuniZAP-265114 as template and BamHI and SmaI sites...

16
754
0