... between zebrafish and human, mouse and pufferfish B-FABP is 83%, 76% and 83%, respectively The percentage amino acid identity between zebrafish and human and mouse is higher in the exons and than it ... specific patterns of expression in developing neural tissues [33] and in the adult brain [34] B-FABP is specifically expressed in the mammalian and zebrafish brain [11,13,15,17], and its expression correlates ... shows the ratio of B-FABP mRNA to b-actin mRNA in various tissues with abundant expression of the B-FABP mRNA seen in RNA extracted from adult brain (B), much lower B-FABP mRNA levels in testis (T),...
... decrease in mRNAexpression with increasing RWJ 67657 concentration TNF-α mRNAexpression is inhibited by 48.4% at 0.01 µM and 65.8% at 0.1 µM p38 MAPK inhibitor IL-1β, IL-8, and COX-2 mRNAexpression ... IL-6 production andmRNAexpression were not inhibited by p38 MAPK inhibition, but IL-8 production andmRNAexpression were inhibited by more than 50% at µM RWJ 67657 Bhattacharyya and co-workers ... in transcription and stabilization of COX-2 mRNA [23] However, Caivano and Cohen showed that both p38 MAPK and ERK influence COX-2 mRNAexpression through activation of mitogen- and stress-activated...
... a doubling of expression, and a fold change of 0.5 meaning a halving of expression) Statistical and power calculations are reported in Table Figure Graph illustrating the means and 95% confidence ... TIMP4 and an increase in the expression of TIMP1 in canine OA cartilage The decrease in TIMP4 expression was consistent with expression profiles of human OA cartilage [39], although TIMP1 expression ... http://arthritis-research.com/content/8/6/R158 Table Change in gene expression, mean 2-ΔΔCT values, significance and power of comparisons between normal and OA canine articular cartilage Number of samples in which expression was detected...
... Kinne RW: Comparison of conventional and real-time RT-PCR for the quantitation of jun protooncogene mRNAand analysis of junB mRNAexpression in synovial membranes and isolated synovial fibroblasts ... reading of the manuscript and Bärbel Ukena, Ulrike Körner, and Ildiko Toth for excellent technical assistance We are grateful to Andreas Roth, Rando Winter, Renée Fuhrmann, and Rudolf-Albrecht Venbrocks ... the basis of genome-wide mRNAexpression data in RA (n = 12), osteoarthritis (OA) (n = 10), and normal control (NC) (n = 9) synovial tissue Materials and methods Patients and tissue samples SM samples...
... differential expression between ER- and ER+ samples (b) Expression levels of Ago1, Ago2, Dicer, and TRBP in ER+ (red) and ER- (green) samples The mean and the standard deviation of the expression ... in ER+ and ER- samples (Additional data file 4) 19 adjusted arelevels(FDR)targetused ,and 210containing expression Expressionsheets, 1,563, 765, -0.20' and for expression comparison miRandaP-value ... and 2,026 targets per miRNA are predicted in ConservedTS, ContextTS and AllTS, respectively After integrating mRNAexpression data with all three target sets to compare the RE-scores in ER- and...
... in miRNA expression between the normal mammary glands and mammary tumors, we compared the miRNA expression profiles across all of the murine tumor models and normal mammary glands miRNA expression ... miRNA andmRNA gene expression analysis, we demonstrated in vivo that the expression of miRNAs can be associated with the inverse expression of a subset of predicted target mRNAs in mammary gland ... Figure Over -expression of (a) miR-494 and (b) miR-412 inhibits expression of Birc4 and Bmpr1a, respectively M6 cells and DB-7 cells were transduced with lentivirus expressing miR-494 and miR-412,...
... in miRNA expression between the normal mammary glands and mammary tumors, we compared the miRNA expression profiles across all of the murine tumor models and normal mammary glands miRNA expression ... miRNA andmRNA gene expression analysis, we demonstrated in vivo that the expression of miRNAs can be associated with the inverse expression of a subset of predicted target mRNAs in mammary gland ... Figure Over -expression of (a) miR-494 and (b) miR-412 inhibits expression of Birc4 and Bmpr1a, respectively M6 cells and DB-7 cells were transduced with lentivirus expressing miR-494 and miR-412,...
... (A, B and C, respectively) Panels G, H and I show the differential gene expression of the mRNA for collagens I, III and V, respectively, in lung tissue of control and immunized animals and after ... scleroderma model and nasal tolerance and the immunofluorescence experiments, and drafted the manuscript; WRT contributed to conception and design of the original study and analysis and interpretation ... chain Complete primary structure, expression domains in adult and developing tissues, and comparison to the structures andexpression domains of the other types V and XI procollagen chains J Biol...
... liver [29] and reduced expression of hepatic mRNA of GHRs have been demonstrated [14] Reduced receptor binding andmRNA was found following endotoxin challenge [30], whilst unchanged GHR mRNA was ... is 14 pg/ml, and that for IL-10 is
... NLgag: Gag mRNA Total poly-A mRNA C191G A192C C193G A194G -C ert 26 ns Em2 E TEm i CT R No RT LTR GFP mRNA p24gag (Fold Induction) Fold increase in: gag mRNA Gag protein Gag mRNA GFP mRNA RTEm26 ... experiments in using infectious HIV and SIV and provided technical assistance; ASZ and CM provided reagents and intellectual input; GNP provided intellectual input and contributed to 13 14 Grüter ... within env and is expressed from the HIV-1 LTR promoter RTE, CTE and RTEm26-CTE were inserted between the env gene and the 3' LTR B) HLtat cells were transfected with the indicated plasmids and analyzed...
... IFNγ-inducible CXCR3 ligands and some CCR5 ligands are increased in autoimmune diseases [11-14] However, other findings show that no correlation exists between Th1/Th2 cytokine profile and chemokine ... CCR5, CCR8 andCXCR3 in memory Th cells from allergic, asymptomatically sensitized and healthy individuals to obtain knowledge about their migratory potential and any differences in expression ... other studies reporting equal mRNA levels of CCR3 and CCR5 in PBMCs [22] and same levels of CXCR3+ peripheral blood Th cells [21] in patients with atopic dermatitis and healthy controls, but in...
... 11.05 ± 1.94 Figure TNF-α expression (a) In liver after 48 and 96 hours Relative mRNAexpressionexpression of TNF-α in liver tissue, detected by semi-quantitative RTPCR 48 and 96 hours after the ... TNF-α mRNA against the amount of mRNA of the housekeeping gene GAPDH * P ≤ 0.05 The data are expressed as mean ± standard error of the mean (b) In lung after 48 and 96 hours Relative mRNAexpression ... of femur fracture/hemorrhage and subsequent sepsis Special focus of the study was the cytokine mRNAexpression pattern in two organ compartments (liver and lung) 48 and 96 hours after sepsis induction...
... predicted effects on mRNAexpressionand miRNA expression levels detected by miRNA microarrays Correlation Correlation between miRNAs with predicted effects on mRNAexpressionand miRNA expression levels ... with predicted effects on mRNAexpressionand miRNA expression levels detected by RT-PCR Correlation between miRNAs with predicted effects on mRNAexpressionand miRNA expression levels detected ... gene expression profiles Materials and methods Stand Alone Java and R programs [43], in conjugation with MS Access and MS Excel, were used for the following analyses Gene expression data Mouse and...
... hematopoiesis Tissues with high gene set expression levels include fetal, but not adult, liver and kidney, and lung and bone marrow Expression was also noted in placenta Expression of hemoglobin genes ... [21] els in testis and subregions of adult brain Lower expression of the ribosome in non-proliferative tissues is expected, and the similarity of ribosomal expression in brain and testis has been ... normal tissues and cancer cell lines A high-resolution version of this map and all expression data are freely available [21] The resulting map of the expression of human pathways and other gene...
... of ammonia oxidizers and copy number of amoA mRNA The time courses of the copy number of amoA mRNAand the number of ammonia oxidizer are shown in Fig No significant change and difference in the ... other hand, significant change and obvious difference in the amount of amoA mRNA were observed in each run Vertical axis of this graph shows amoA mRNA copy number in a PCR-reaction tube, and the ... on amoA mRNAexpression is limited, and dosage of polyferric sulfate must be optimized not only for phosphorus removal but also for ammonia oxidation Although the possibility that amoA mRNA would...
... strand Both the strands can be used as templates for DNA sequencing reactions The immobilized strand can be stored and reused for further reactions into solution and leaving the other strand ... DNA from a primer annealed to a single-strand template DNA molecule In general for PCR products the template will be doublestranded and is heat denatured and rapidly frozen by placing in dry ice ... containing nonpolymerized gel matrices and laser detection systems The introduction and removal of polymer from the capillaries, plus loading and running samples and fragment detection, are automated...
... most common form and content of modality As a result, a full and specific description of syntactic and semantic features of English modal verbs can, may, must and the equivalent expressions in ... constant encouragement and the favourable conditions spared for my study Finally, my warmest thanks go to my parents, my husband for their love, support and share of housework and childcare Without ... reading skill and in doing some English-Vietnamese translation I found that students still have many problems in understanding the texts, and especially in interpreting the writers opinion and attitude...
... policy and practice and awareness raising Finally, Section provides an overview of literature, background material and tools on international and national policy and practice on privacy and freedom ... of expressionand privacy Research on safeguarding the principle of freedom of expression in Internet policy across a range of issues will continue to be part of UNESCO’s normative mandate and ... surveillance; Deep packet inspection (DPI); and data processing and facial recognition and surveillance technology International legal standards on privacy, and responses to these emerging issues,...
... Briefly, short-mir-9 and MPfwd ⁄ MP-rev primers (Table S1) were used at concentrations of and 100 nm, respectively U6 and ARBP were used for the normalization of mir andmRNA expression, respectively ... and 60 nM of precursors of mir-control, mir-9 and mir-34a b-Actin was used as a loading control The relative protein levels were quantified using Adobe Photoshop and are indicated Sirt1 mRNAand ... of mir-control and mir-9 (A) and 1.5 lg of pSuper and pSuper-mir-9 plasmids (B, C) for 72 and 24 h, respectively (D) b-TC-6 cells were cultured in high-glucose-containing medium and subjected...
... property and traditional cultural expressions Wend B Wendland The lay of the land: the geography of traditional cultural expression Johanna Gibson v 123 150 182 vi Contents PART 4: NEW TECHNOLOGIES AND ... technologies and the protection and promotion of traditional cultural expressions Mira Burri-Nenova 10 New information and communication technologies, traditional cultural expressions and intellectual ... Latour, Visualization and Cognition: Thinking with Eyes and Hands (1986) Knowledge and Society Studies in the Sociology of Culture Past and Present 6, pp 140 Local traditions and global law Convention...