cu zn superoxide dismutase induced mitochondrial dysfunction in amyotrophic lateral sclerosis
báo cáo hóa học: " Formation of multinucleated giant cells and microglial degeneration in rats expressing a mutant Cu/Zn superoxide dismutase gene" ppt
Ngày tải lên :
19/06/2014, 22:20
... rinsed in PBS and incubated in secondary antibody (1:500) for h Following incubation, slides were rinsed and Horseradish Peroxidase Avidin D was applied (1:500; Vector, Burlingame, CA) and incubated ... Lectinanimals (B-H) Figure stage staining of microglia in the brainstem (level of cranial nerve VII) in wildtype animals (A) and in late symptomatic/end Lectin staining of microglia in the brainstem ... RP, et al.: Amyotrophic lateral sclerosis and structural defects in Cu, Zn superoxide dismutase Science 1993, 261(5124):1047-1051 Siddique T, Deng HX: Genetics of amyotrophic lateral sclerosis Hum...
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Báo cáo khoa học: "Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis" ppsx
Ngày tải lên :
07/08/2014, 20:23
... times in PBS, for each time Nonspecific binding was blocked with a 20 incubation in diluted o normal serum, and sections were incubated for h at 37 C with the SOD1 antibody Next, sections were incubated ... SOD1 was strongly expressed in the basal cells and muscular layers of the stomach on ED 16.5 (Fig 2R) In developing intestine, SOD1 expression was moderate in the intestinal villi and muscle layers ... the SOD1 signal was higher in the acinus than the islet of the Table Comparison of cytoplasmic Cu/ Zn superoxide dismutase expression in developing embryonic organs following embryonic day Organs...
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Báo cáo lâm nghiệp: "Salicylic acid up-regulates the expression of chloroplastic Cu, Zn-superoxide dismutase in needles of maritime pine (Pinus pinaster Ait.)" pptx
Ngày tải lên :
08/08/2014, 01:22
... form of Cu, Zn- SOD from Pinus pinaster MATERIALS AND METHODS Figure Amino acid sequence analysis of P pinaster chloroplastic Cu, Zn- SOD with other chloroplastic (chl) and cytosolic (cyt) Cu, ZnSODs ... DISCUSSION 2.4 cDNA library screening and sequence analysis of Cu, Zn- SOD cDNA Screening of P pinaster cDNA library for Cu, Zn- sod cDNA clones was performed using the Cu, Zn- sod cDNA from Zantedeschia ... [14]), differing in their active site metal ion (Cu/ Zn, Fe and Mn) Cu, Zn- SOD isoforms are structurally unrelated to the remaining groups; most of them form an homodimer and each subunit binds a copper...
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Báo cáo y học: "Transduction of Cu, Zn-superoxide dismutase mediated by an HIV-1 Tat protein basic domain into human chondrocytes" docx
Ngày tải lên :
09/08/2014, 08:22
... anti-human Cu, Zn- SOD IgG in cartilage slices treated with µM of Tat-SOD or control SOD; Cu, Zn- SOD was detected in the explant culture with both treatments (Figure 2b) While the intensity of staining ... hydrogen peroxide and superoxide, but that only superoxide mediated IL-1 induced NF-kB activation and iNOS expression [20] The investigators did not report whether IL-1 induced ROS or superoxide is responsible ... PTD in potential therapeutic development are increasing in various fields and in a variety of cell systems and include inhibition of apoptosis by transduction of anti-apoptotic Bcl-XL protein in...
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Báo cáo y học: " Vgf is a novel biomarker associated with muscle weakness in amyotrophic lateral sclerosis (ALS), with a potential role in disease pathogenesis"
Ngày tải lên :
03/11/2012, 10:52
... C-terminal epitope of Vgf Unoccupied binding sites on the plates were blocked by incubation with casein Samples and standards were applied in duplicate and incubated overnight at 4°C Following ... tissue staining intensities influenced the measured values Average value density measurements from individual Vgf immunoreactive dorsal spinal cord neurons, reflecting immunostaining intensity, ... delimiting the cellular area of interest free hand, using predetermined criteria to define the region of interest The immune intensity of the cellular Vgf encompassing the L3-L5 regions of spinal...
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Báo cáo khoa học: Proteomic characterization of lipid raft proteins in amyotrophic lateral sclerosis mouse spinal cord pot
Ngày tải lên :
23/03/2014, 04:21
... family member Destrin (actin-depolymerizing factor) EF-hand domain containing protein 2, Swiprosin-1 Ezrin (p81) (cytovillin) (villin-2) Heat shock 27 kDa protein Integrin a-M precursor Lysosome-associated ... precursor Serine ⁄ threonine-protein phosphatase 2A catalytic subunit SH3 adapter protein SPIN90 (NCK-interacting protein with SH3 domain) Sushi domain-containing protein precursor FEBS Journal 276 ... Mutations in Cu ⁄ Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis Nature 362, 59–62 Pasinelli P & Brown RH (2006) Molecular biology of amyotrophic lateral sclerosis: ...
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Reducing pathological accumulations of phosphorylated neurofilament h through modulation of pin1 activity implications in amyotrophic lateral sclerosis
Ngày tải lên :
10/09/2015, 09:29
... immunohistochemistry Interleukin induced nitric oxide synthase enzyme inositol 1,4,5-triphosphate receptor gene inverted terminal repeats JNK-interacting protein c-Jun N-terminal kinase Luria Bertani ... protein kinase N Polo-like kinase protein phosphatase protein phosphatase A phophatases Peptidyl-prolyl isomerases Proline phosphorylated serine/threonine-proline recombinant AAV RNA-interfering ... short hairpin RNA small interfering RNAs Spinal Muscular Atrophy survival motor neuron single-nucleotide polymorphism superoxide dismutase TAR BD Binding Protein tris buffered saline T-cell transcription...
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Báo cáo khoa học: "Bench-to-bedside review: Potential strategies to protect or reverse mitochondrial dysfunction in sepsis-induced organ failure" pps
Ngày tải lên :
13/08/2014, 02:24
... recovery process This increasing insight into underlying mechanisms promises to be an exciting era of novel therapeutic developments Competing interests MS receives research funding from the Medical ... leptin levels are increased in survivors of acute sepsis: associated loss of diurnal rhythm, in cortisol and leptin secretion J Clin Endocrinol Metab 1998, 83:280-283 20 Callahan LA, Supinski ... A, Hodin RA, Fink MP: Inhibition of inducible nitric oxide synthase ameliorates endotoxin -induced gut mucosal barrier dysfunction in rats Gastroenterology 1997, 113:1246-1257 King CJ, Tytgat...
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Báo cáo y học: " Corticosteroid effects on ventilator-induced diaphragm dysfunction in anesthetized rats depend on the dose administered" pptx
Ngày tải lên :
12/08/2014, 13:22
... ischemia -induced liver injury, pretreatment with prednisolone (10 mg/kg, corresponding ~1.6 mg/kg in humans) abolished calpain activation in the liver [14] Interestingly, in this study the calpaininhibiting ... diaphragm [15] Therefore protection against CMV -induced increases in intracellular calcium levels and/or increasing calpastatin binding to calpain could be potential mechanisms by which corticosteroids ... the MP treatment inhibited CMV -induced calpain activation in the diaphragm by preventing an increase in cytosolic Ca 2+ levels, preservation of calpastatin levels, or some combination of both...
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Báo cáo khoa học: "Endotoxin-induced myocardial dysfunction in senescent rats" docx
Ngày tải lên :
13/08/2014, 03:20
... body weight and main organ weight in response to 12-hour endotoxemia in each age group are summarized in Table In senescent rats, LPS induced a body weight loss and an increase in heart weight ... in rats The main findings were the following: 12 hours after LPS injection (0.5 mg/kg), a marked reduction in myocardial contractility was observed in the isolated perfused senescent heart; in ... of the acute ventricular dilation reported in septic patients [9,38] Indeed, a reduction in myofilament responsiveness is associated with increased length in single cardiac myocytes and increased...
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