... quantified by densitometry and expressed as a fold difference against actin by a concomitant accumulation in the G1 phase cell population (28.4% in control vs 63.1% and 74% in 2.5 and mM LA treated cells) ... Fig 1A) By 48 h there was a ~8%, ~64% and 86% diminution of cell growth by 1, 2.5 and mM LA, respectively, which was accompanied by ~1, ~3% and LA induces HL-60 cell cycle arrest by altering the ... distribution and the expression of various cell cycle regulatory proteins in HL-60 cells (A) Cells were treated with 0, 2.5 and mM LA for 24 and 48 h and analyzed by flow cytometry Cells with hypodiploid...
... and IL-17 production by CD4+ T cells (Intracellular cytokine staining) 116 X LIST OF ABBREVIATIONS Nucleotides containing adenine, thymine, cytosine, and guanine and abbreviated as A, T, C, and ... both innate immunityand adaptive immunity are summarized in Table 1.1 Table 1.1 Comparison of Innate and Adaptive Immunity Properties Innate Immunity Effector Cells • Adaptive Immunity NK cells, ... contain and remove the invading pathogen 1.2.2 Myeloid Cells form a Major Arm of Innate Immunity The cellular component in innate immunity formed by myeloid cells is a major arm of the innate...
... potent pro-inflammatory cytokines and chemokines The hall-mark cytokines involved in initiation of the adaptive Th1 immune responses are IL-12p70 whereas IL-6, TGFb and IL-23 are involved in initiation ... nucleotide binding and oligomerization domain-like receptors (NLRs) and C-type lectin-like receptors (CLRs) [3] These receptors and their corresponding signalling pathways are involved in the pathology ... like nitric oxide, prostaglandins and chemokines [1,2] During inflammation, immature DCs are attracted to the site of inflammation by chemokines in the microenvironment, and a large number of DCs...
... and Vaccines 2004, 2:8 DCI E-64 100 80 40 20 Cathepsin D/E Cathepsin C Cathepsin A Chymotrypsin BLT Esterase Cathepsin L Cathepsin J Cathepsin H 0 Cathepsin A Cathepsin L Cathepsin J Cathepsin ... Cathepsin H Cathepsin D/E Cathepsin C Chymotrypsin BLT Esterase Cathepsin A Chymotrypsin BLT Esterase Cathepsin L Cathepsin J Cathepsin H Cathepsin D/E Cathepsin C 20 20 40 60 60 40 % Inhibition 80 ... and functional analysis of antigen processing indendriticcellsand macrophages Immunology 1986, 58:271-280 Rhodes J, Andersen A: Role of cathepsin D in the degradation of human serum albumin...
... (Figure 1) They include structural matrix proteins (collagens, laminin, fibronectin, fibrin, etc.) and molecules modulating cell migration (integrins), cell-cell and cell-substrate interactions ... collagen fiber assembly and structure Types II and VI collagen bind matrix receptors (integrins) Type II collagen can also bind to anchorin CII, whereas fibronectin binds to integrins A large proteoglycan ... networks containing a cell-binding site for ligation of cell-surface integrin receptors and substrates for MMPs, proteases implicated in wound healing and tissue regeneration [51,52] and fabrication...
... suppressors Influenza virus NS1 and vaccinia E3L proteins, two inhibitors of the IFN-induced protein kinase R (PKR), inhibit RNAi pathways in plants andin Drosophila cells [12] HIV- 1 Tat protein acts ... proteins with two dsRBDs and a KR-helix motif within dsRBD2 that mediates RNA binding A third C-terminal basic domain does not mediate RNA binding [20,21] TRBP is a strong PKR inhibitor by direct ... Gatignol A: Two dimerization domains in the Trans-activation response RNA-binding Protein (TRBP) individually reverse the Protein Kinase R inhibition of HIV- 1 Long Terminal Repeat expression J Biol...
... ligands 29 Chapter 1: Introduction TLRs are mainly expressed by APCs, including DCs, macrophages, monocytes and B cells They are also expressed by T cellsand non-immune cells like epithelial cells ... antibody per million cells) , and mixed by raking gently on tube racks Cells were incubated in the dark at 4oC for 30 mins After incubation, cells were washed by adding ml FACS buffer and centrifugation ... cytokine consisting of covalently linked p35 and p40 subunits The genes encoding the p35 and p40 are located on separate chromosomes, chromosome andin humans, and chromosome and 11 in mice (Trinchieri,...
... (IFN)-regulatory factor (IRF3) and the induction of IFN- are observed in a MyD88independent manner A third TIR-domain-containing adaptor, TRIF (TIR-domain-containing adaptor protein inducing IFN-), is essential ... immune cells, dendriticcells are the most potent activators of adaptive immunity 1.3.1 DendriticcellsDendriticcells (DCs) are the key cell types involved in the connection between the innate and ... normal intestinal flora and are isolated in 11% of healthy individuals (17) They not cause gastroenteritis, but their main feature is their ability to colonize extraintestinal sites and to induce infections...
... direct binding andby binding to opsonins on the microbe surface Receptor engagement induces many intracellular signals, and several molecules are utilized in many pathways Signaling during phagocytosis ... regulation of innate immunity, TLRs appear to be directly involved in induction of antimicrobial activity Microbial lipoproteins induce iNOS gene transcription via TLR2, indicated by the induced iNOS ... (LRR) and carboxyl-terminal Toll/interleukin-1 receptor (TIR) homology domain (Rock et al., 1998) LRRs are found in a diverse set of proteins in which they are involved in ligand recognition and...
... acidity, integrated pest management, double-cropping, and crop Livestock’s rolein climate change and air pollution rotations including green manure and cover crops Increasing crop yields result in ... Burning is common in establishing and managing burning represented some 85 percent of the area of pastures, tropical rain forests and savannah burned in Latin American fires 2000, 60 percent in ... conversion to appropriate land uses, and adoption of recommended management practices on cropland and grazing land.” Taking just the grasslands in Africa, if the gains in soil carbon stocks, technologically...
... exon and exon 1a, we compared the promoters involved in Chang and Huh7 cells As shown in Fig 1D, PCR products containing exon were produced in Huh7 cells but not in Chang cells To determine whether ... Chang cells Furthermore, in a comparative analysis between OccWT and OccDE9, the exon region played a significant rolein promoting apoptosis and inhibiting invasion by regulating signaling pathways ... localized in a more disconnected fashion than that in Hep3B cellsIn Chang cells, stained occludin was observed in the cytoplasmic region Additionally, in OccWT-overexpressing Hep3B and Huh7 cells, ...
... (Michaelis constant) and Vmax (maximum velocity) values were determined by incubating the diluted recombinant proteins in the standard reaction mixture in the presence of increasing concentrations ... were removed and minced with a surgical knife The minced tissue was wrapped in cotton gauze and suspended in NaCl/Pi containing 100 lgÆmL)1 penicillin/ streptomycin at 37 °C for h After incubation ... were incubated at 37 °C in a humidified 5% CO2 incubator in the absence (control) and presence of increasing concentrations of inhibitors for 10 days, and the number of molting larvae was determined...
... functions including apoptosis in infected and uninfected bystander target cells through its interaction with host cellular proteins [22–25] However, it is not clear at this point how interaction ... (RTK) signaling, independent of MAP kinase (MAPK) by either functioning downstream of MAPK or by defining a new pathway of RTK signaling [8] RTKs play important roles in cell signaling during cell ... Cancer Institute, Shanghai, China Smith RK, Carroll PM, Allard JD & Simon MA (2000) MASK, a large ankyrin repeat and KH domain-containing protein involved in Drosophila receptor tyrosine kinase...
... mutation in the pks15 ⁄ gene, and production of PGL-tb can be restored by introducing a functional pks15 ⁄ gene into these strains [13] Accordingly, both the PMM74 and wild-type strains were ... 7H11 plates containing either kanamycin (Km) or hygromycin (Hyg) Mice were killed or 21 days postinfection, and the mutant and wild-type loads in lungs and spleens were determined on the basis ... be explained by the different genetic backgrounds of the M tuberculosis strains used in these studies or ⁄ andby different experimental conditions Indeed, Cox et al [3] infected mice by intravenous...
... based in Uganda and Rwanda, andin preventing Angola, Namibia and Zimbabwe from sending in troops to prop up his shaky regime South Africa’s intervention in Lesotho in September 1998, in the wake ... and Guinea-Bissau; crises in Guinea-Conakry and Guinea-Bissau; and internationalised civil wars in Sierra Leone and Liberia ECOWAS, or coalitions of its members, have played important roles in ... has inexorably drawn it into extensive involvements in conflict resolution and peacemaking Some of these, notably in Lesotho and Zimbabwe, have been dictated by immediate selfinterest and propinquity...
... published in ‘Believing by Faith and the Concept of God’, in Ree Bodd´ and Hugh Kempster (eds), Thinking Outside the Square: Church in e Middle Earth (Auckland: St Columba’s Press and Journeyings, ... epistemology is set out in essays by William Alston, Alvin Plantinga, and Nicholas Wolterstorff in Alvin Plantinga and Nicholas Wolterstorff (eds), Faith and Rationality: Reason and Belief in God (Notre ... that kind of justification, of course Some beliefs might count as justified in the sense that holding and acting on them serves the believer’s interests, or in the sense that holding and acting on...
... days in 4% paraformaldehyde and embedded in paraffin Sections were de-waxed and stained with haematoxylin and eosin for histological examination Toluidine blue staining was performed to assess ... saline (PBS) and then rinsed thrice with PBS Non-specific binding was blocked with 10% normal goat serum (NGS; Zhongshanjinqiao, China) in PBS for 10 minutes Cells were washed with 1% NGS in ... cells cultured on poly-L-ornithine- and laminin-coated coverslips displaying a microglial morphology derived Immunofluorescence staining of β-tubulin, glial fibrillary acidic protein (GFAP) and...
... via TRIF (TIRdomain-containing adapter-inducing interferon-b) joining the RIG-I/MDA5 pathway In the other pathway, TLR7 senses single-strand HCV RNA and via the MyD88 adaptor protein activates IRAK4/IRAK1 ... HCC byin vitro andin vivo studies, both in animal models andin humans In particular, from some studies, conducted both in vivo andin vitro, resveratrol emerged as a promising molecule that inhibits ... CD94/NKG2A on NK cellsin HCV-infected patients In addition, dendriticcells (DC) sense virus infection via toll-like receptors (TLR) or retinoic acid inducible gene-I (RIG-I), resulting in the secretion...
... than in OA and AS synovium CD147 was expressed predominantly on the macrophage-like cellsand fibroblast-like synovial cellsin the lining and sublining layers (Fig 2) MMP-1 was expressed predominantly ... monocytes and fibroblasts by 28.9 ± 5.9% in the normal control group andby 22.8 ± 3.8% in the RA group AP9 inhibited cell invasion by 55.1 ± 4.5% in the co-culture of undifferentiated THP-1 cellsand ... (green) and CD147+ (red) cells, and the co-expression of CD68+CD147+ cells (yellow) in the lining and sublining layers of rheumatoid arthritis synovium is apparent Immunohistochemistry staining of...
... studies in determining the presence, enumeration and function of each subset in PB and SF IFN-α was detected in SF in both RA patients and PsA patients The role for this cytokine in synovial pathogenesis ... IgG2a-PE and mouse IgG1-FITC antibodies A proprietary dead cell discriminator was also included After incubation, red cell lysis and washing, cells were fixed and examined using a FACScalibur and ... inversely correlated to CRP The significant decrease of pDCs and mDCs in RA PB and of pDCs in PsA PB, as well as the trend towards a decrease in pDCs in OA PB, were intriguing and led us to investigate...