coupled enzyme activity and thermal shift screening of the maybridge rule of 3 fragment library

Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution ppt

Ngày tải lên : 07/03/2014, 00:20

... of the enzyme in this study In addition, the structural rationale for the other stability (E83K and T407A) and activity (T129I and T451A) mutations was investigated on the basis of the open and ... study, the A13V, E83K and T407A mutations originating from the S1–S3 hits were repeatedly detected during the screening of the reassembly library (Table 1) and, of these mutations, A13V was detected ... origin of the mutations to be estimated For example, AS4, composed of A13V, E83K and T451A, was analyzed to be a reassembly of S3 (harboring the A13V mutation), S2 (harboring the E83K mutation) and...

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Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding afﬁnity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx

Ngày tải lên : 14/02/2014, 19:20

... 1 23 32 0.4 0.5 47–51 44–50 43 47 43 47 43 49 40–45 39 –41 43 47 43 47 44–50 46–51 °C °C °C °C °C °C °C °C °C °C °C 3. 4–4.1 3. 4–4.1 3. 4 3. 8 3. 4–4.1 3. 6–4.1 3. 4–4.1 3. 8 3. 8 3. 5–4.1 3. 6–4.1 3. 5–4.1 ... some of the XBS mutants Activity measurements After the screening procedure, a smaller set of enzymes was selected for puriﬁcation and further biochemical characterization The activity of these enzymes ... xylanases Table The effect of genetic engineering of the secondary binding site of the B subtilis and A niger xylanases on the screening ratio The screening ratio is deﬁned as the ratio of activity...

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Physical Activity and Health: A Report of the Surgeon General pdf

Ngày tải lên : 14/03/2014, 20:20

... to the age of the individual, to the season of the year, to the changes in the winds, to the situation of the region in which the patient resides, and to the constitution of the year (19 53 reprint, ... physical activity, the report was planned collaboratively with representatives from the Office of the Surgeon General, the Office of Public Health and Science (Office of the Secretary), the Office of ... the Chemeheuvi of California, the Inca of Peru, the Zuni and other Pueblo peoples of the American Southwest, and the Iroquois of the American East, who also developed the precursor of modern-day...

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Báo cáo khoa học: Inﬂuence of divalent cations on the structural thermostability and thermal inactivation kinetics of class II xylose isomerases pdf

Ngày tải lên : 23/03/2014, 13:20

... cations and amino acid composition contribute FEBS Journal 272 (2005) 1454–1464 ª 2005 FEBS Thermostability of class II xylose isomerases 27 28 29 30 31 32 33 34 35 36 37 to the thermostability of ... reasons In the presence of Mg2+ and Co2+, D309K TNXI has a Tm (95.5 °C) slightly lower than that of the apoenzyme, and E323K TNXI has the same Tm as TNXI in the presence of a single metal These results ... explains the similar biochemical and thermostability properties of these enzymes [7] Comparisons between the structures of the most thermostable class I XIs from Thermus caldophilus and Thermus thermophilus...

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cross plane electronic and thermal transport properties of p type la0.67sr0.33mno3 lamno3 perovskite oxide metal semiconductor superlattices

Ngày tải lên : 06/05/2014, 08:53

... cross-plane thermal and electrical transport by suppressing the effects of parasitic electrical series resistance and the electronic contributions to the thermal conductivity The results suggest that the ... La0.67Sr0 .33 MnO3 is based on LaMnO3 (Mn3þ, t32ge1g) as the parent compound, where La3þ is partially replaced by Sr2þ, which forces a partial change of Mn3þ to Mn4þ with no eg electron (t32ge0g), ... measurement of the cross-plane Seebeck coefﬁcient using a thermal imaging technique .37 The measured value was 1520 53 lV/K Combining the cross-plane measurements of the LSMO/LMO superlattices at 30 0...

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electrical and thermal interface conductance of carbon nanotubes grown under dc bias voltage

Ngày tải lên : 06/05/2014, 08:53

... indicates the existence of multiple G peaks The quality of the ﬁt in the low-energy and high-energy tails of the D- and G-bands, respectively, is good The assignment of the peak components of the D- and ... evaluate the quality of the CNTs Yoshida et al .32 have shown that the FWHM intensity of the G-band reﬂects the degree of graphitization of carbon materials The effect of dc bias voltage on the IG/ID ... CNTs to the differences in the quality and the surface morphology of the CNTs The variation in the thermal interface resistance of the CNT arrays is attributed primarily to the effect of bias...

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thermomechanical and thermal contact characteristics of bismuth telluride films electrodeposited on carbon nanotube arrays

Ngày tải lên : 06/05/2014, 08:58

... increases the total thermal interface resistance, the contribution of the CNT interfacial layer to the total thermal resistance is expected to be $11% of the thermal resistance of (Bi0.7,Sb1 .3) (Se2.91,Te0.09) ... calculated at the perimeter of the Bi2Te3 film, assuming zero lateral displacement at the center The maximum magnitude of the calculated stress-free displacement at the edge of the film with the CNT ... Bi2Te3 films of 100 mm thickness, the measured thermal interface resistances of CNT-TE samples is $55% of the intrinsic resistance of the TE film itself (0.55 K WÀ1, corresponding to a measured thermal...

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báo cáo hóa học:" Physical activity and change in quality of life during menopause- an 8-year follow-up study" docx

Ngày tải lên : 20/06/2014, 16:20

... hours/week 0.001 21-42 249 21.6 32 2 27.9 >42 665 57.7 677 58.6 0.068 BMI (kg/m2 )3) 30 2 63 22.8 2 63 23. 3 >0.001 Menopausal ... box 30 33 501 Tampere Finland e-mail riitta.luoto@uta.fi Abstract Background and objectives The aim of this study was to study the role of menopausal status and physical activity on quality of ... women On the other hand, lean women have more hot flushes at the time of menopause [30 ] and they have been shown to use HRT more frequently [31 ], although opposite results also exist [32 ] Stadberg...

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báo cáo hóa học:" Recommended level of physical activity and health-related quality of life among Japanese adults" pot

Ngày tải lên : 20/06/2014, 16:20

... 39 (45 .35 ) 64 (42 .38 ) 54 (47.79) 78 (52 .35 ) 31 ( 53. 45) (38 .89) 20 ( 23. 26) 47 (31 . 13) 31 (27. 43) 33 (22.15) ( 13. 79) (27.78) 18 (20.22) 41 ( 23. 03) 29 (24.17) 22 (18.18) 13 ( 23. 64) (21. 43) 40 (44.94) ... 25.04 39 .22(9.90) 126 21.84 40.12(11.86) 276 47. 83 39.46(10.49) 175 30 .33 38 .34 (10.94) 54 (36 .99) 28 (19.45) 33 (22.92) 43 (30 .50) 59 (40.41) 77 ( 53. 47) 70 (48.61) 67 (47.52) 33 (22.60) 39 (27.08) ... (25.00) 43 (22.51) 45 (20.55) 38 ( 23. 90) (00.00) 104 (54.45) 109 (49.77) 58 (36 .48) (62.50) 44 ( 23. 04) 65 (29.68) 63 (39 .62) (37 .50) 27 (31 .40) 40 (26.49) 28 (24.78) 38 (25.50) 19 (32 .76) (33 .33 ) 39 ...

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Báo cáo hóa học: " Thermal conductivity and thermal boundary resistance of nanostructures" pdf

Ngày tải lên : 21/06/2014, 04:20

... explain the further increase of the thermal conductivity when the roughness is large enough The behaviour of the cross-plane thermal conductivity is different: it increases monotonously with the ... W/mK At the low temperatures considered (T = 0.15 LJU), the period of the superlattice is comparable to that of the PMFP The qualitative interpretation of the results shows that the thermal contact ... micro-superlattices is given There are voids at the bottom of the trenches which are explained by the absence of the seed layer at the bottom, and the fact that they prevent any copper growth These voids were...

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Báo cáo y học: "Physical function, disease activity, and health-related quality-of-life outcomes after 3 years of adalimumab treatment in patients with ankylosing spondylitis" potx

Ngày tải lên : 09/08/2014, 14:22

... 2 73 268 261 241 235 15.6 ± 20.99 b 19.5 ± 21 .38 21.8 ± 21.79 21.9 ± 22.26 22.2 ± 22 .34 23. 3 ± 21.94 288 b 272 270 2 63 237 232 30 .3 ± 40.48 b 37 .2 ± 40.81 35 .2 ± 40.72 39 .1 ± 41.75 36 .1 ± 42 .39 ... 262 242 236 -3. 1 ± 2.42 -3. 4 ± 2.41 -3. 5 ± 2.55 -3. 8 ± 2 .33 -3. 9 ± 2.44 -3. 9 ± 2 .39 -3. 9 ± 3. 39 288 b 274 270 261 242 236 -2.2 ± 1.91 b -2.6 ± 2.04 -2.8 ± 2.10 -2.9 ± 2.14 -2.9 ± 2.17 -3. 0 ± 2.10 ... SF -36 Bodily Pain 288 b 274 270 2 63 237 233 24.0 ± 21.78 b 29.0 ± 22.96 29.7 ± 23. 24 29.9 ± 23. 74 31 .8 ± 24.42 31 .7 ± 24 .33 n Mean ± SD change SF -36 General Health 287 b 270 266 260 237 234 8.7...

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Báo cáo y học: " Hyperglycemia alters enzyme activity and cell number in spinal sensory ganglia" ppsx

Ngày tải lên : 10/08/2014, 09:22

... participated in the writing of this manuscript and contributed both intellectually and practically to the content All authors read and approved the final manuscript In DRG and the dorsal horn of the spinal ... suggest that the OVE26 model of chronic hyperglycemia does alter the overall neurochemical profile of the sensory nervous system through cell loss and/ or altered enzyme activity and that this ... glucose levels of at least 400 mg/dl At the histological level, a survey [ 13] of the ratio of small (50 and 500 μm2 area) to large (500 and 1950 μm2 area) primary sensory somata in the fifth lumbar...

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Báo cáo y học: "Identification of an endogenous retroviral envelope gene with fusogenic activity and placenta-specific expression in the rabbit: a new "syncytin" in a third order of mammals" ppt

Ngày tải lên : 12/08/2014, 23:22

... lack of cell-cell fusion observed in vivo at the level of the placenta of the knockout embryos, resulting in impaired maternal-fetal exchanges and death of the embryos at mid-gestation [8] Therefore, ... (scaffold number and ORF-coordinates indicated) were: 131 951(25124:26887), 15025(429:2192 reverse strand), 90528(17499:19256), 1 633 59(7744:9486 reverse strand) for Env-Ory1, and 10 93( 1017 :30 35 reverse ... classically observed for retroviruses Their position and functionality ¢ Figure the the human identified syncytins tions of and mouserabbit Env-Ory1- and Env-Ory2, and of Retroviral envelope protein-based...

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Báo cáo y học: "Physical activity and body composition outcomes of the GreatFun2Run intervention at 20 month follow-up" pptx

Ngày tải lên : 14/08/2014, 08:20

... 9789 .3 (2929.1) 939 7.8 (2559.4) 9579.4 (2 735 .6) 11178.0 (36 62.5) 9452.4 (2654.7) 10278.5 (32 84 .3) 15007.7 (4250.7) 133 93. 3 (35 73. 0) 142 13. 3 (39 98.9) 146 63. 5 (4182.6) 12978.8 (35 07.6) 137 75.0 (39 19.8) ... elements of the programme (e.g., the run/walks, the testing, the wall planner and the website) and although not always recognising them as from the “10 star rules”, they could recall many of the key ... (6.7) 24.7 (8.8) 20.2 (8 .3) 26.7 (6.0) 23. 6 (7.8) Baseline 20.0 (8.5) 25.9 (11.2) 23. 0 (10 .3) 19.1 (8.9) 23. 8 (10 .3) 21.5 (9.9) Follow-up 23. 9 ( 13. 1) 28 .3 ( 13. 3) 26.1 ( 13. 3) 22.5 (11.5) 26.1 (11.9)...

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BÀI báo KHOA học ANTIMICROBIAL ACTIVITY AND PHOSPHORUS RELEASE BEHAVIOR OF STARCH OR CHITOSAN HYDROGEL MEMBRANES

Ngày tải lên : 23/08/2015, 17:21

... investigated the influence of CS on the antibacterial activity of these membranes and the phosphorus release behavior of CRF hydrogels EXPERIMENTAL 2.1 Materials The biopolymers used in the experiments ... initially and became constant after 3 6 days It was due to the high concentration difference between the inside structure of the CRF hydrogel and the outer solution at the beginning of the release ... behaviors of phosphorus in water of hydrogel The phosphorus release behavior of the CRF hydrogel in the deionized water at the room temperature was shown in Figure The release rate of the CRF hydrogel...

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Preparation, morphology and thermal mechanical properties of epoxy nanoclay composites

Ngày tải lên : 16/09/2015, 08:31

... on the scanning electron microscopy (SEM) observations on the fracture surfaces of neat epoxy and the nanocomposites In addition, the hydrothermal effects on the thermal/ mechanical properties of ... density of clay and onium ion surfactant, different arrangements of the onium ions are possible In general, the longer the surfactant chain length, and the higher the charge density of the clay, the ... length of the layers, hence to the aspect ratio of the dispersed nanoparticles Moreover, the degree of delamination of the clay in the polymer matrix, which increases the interaction between the...

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Hinduism And Buddhism, Volume II. (of 3) An Historical Sketch docx

Ngày tải lên : 07/03/2014, 03:20

... features of the Mahayana are the worship of Bodhisattvas and idealist philosophy These are obviously parallel to the worship of Siva and Vishnu, and to the rise of the Vedanta Now the worship of these ... after 450 On the other hand portions of the sutra about Amợda's Paradise, of the Prajủõ-põramitõ, and of the Avatamsaka were translated about 150 A.D and translations of the Lotus and Lalita-vistara ... East[118] and was the fundamental thesis of Bodhidharma, the founder of the Zen school But the practical character of the Chinese and Japanese has led them to attach more importance to the moral and...

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Hinduism and Buddhism, Vol I. (of 3) An Historical Sketch doc

Ngày tải lên : 07/03/2014, 03:20

... He is the guardian of the world, He is the ruler of the world, He is the Lord of the world and He is myself." Here the last words destroy the apparent determinism of the first part of the sentence ... world, the soul and God (the three terms being practically the same) have two modes of existence: one of repose and bliss, the other of struggle and trouble Of these the first mode is the better and ... whereas the pantheism of the Smartas grew out of the feeling that the many gods of tradition must all be one, the pantheism of the Vishnuites was not evolved out of pre-buddhist Brahmanism and is...

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Báo cáo khoa học: Identiﬁcation of calreticulin as a ligand of GABARAP by phage display screening of a peptide library pdf

Ngày tải lên : 16/03/2014, 05:20

... close vicinity to the globular domain of CRT (Fig 5) The sequence segment of the binding motif is not contained in the investigated fragment of the NMR structure of the CRT P-domain The sequence segment ... during the addition of increasing amounts of N1 peptide to 20 lm GABARAP in the presence of 20 lm CRT The addition of N1 resulted in the appearance of new and clearly observable resonances (Fig 3B), ... with the tip of the CRT P-domain [37 ] The carbohydrate binding region is localized within the N-domain [41,42] These interactions are of great importance for the ER functions of CRT; however, they...

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Báo cáo y học: "Anti-inflammatory activity and neutrophil reductions mediated by the JAK1/JAK3 inhibitor, CP-690,550, in rat adjuvant-induced arthritis" ppsx

Ngày tải lên : 11/08/2014, 03:20

... the design the studies and editing of the manuscript DLM participated in the design and coordination of the studies and helped to draft and edit the manuscript All authors read and approved the ... suggest that the reductions in PBNC in human RA patients may be an indirect consequence of the anti-inflammatory activity of CP-690,550 and/ or the inhibition of JAK1 Page 10 of 12 and JAK3 activity, ... relationship between inhibition of JAK1 and JAK3, efficacy, and the inhibition of inflammatory cytokines and neutrophilia in the rat AIA model with CP-690,550 (Tables and 2, Figures and 2) Increases in arthritis,...

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