... the transactivation transcription of the AGT gene in VSMCs Super-EMSA and ChIP confirmed that Ang II could increase the binding activity of STAT5B to the ciselement and the recruitment of STAT5B ... Definitive evidence of physical association between c-Jun and STAT5B is provided by the results of the CoIP and super-EMSA of nuclear extracts, and by the results of the ChIP assay of immunoprecipitated ... means ± SD, and an analysis of variance with Bonferroni’s test was used for the statistical analysis of multiple comparisons of data P-values of less than 0.05 were considered statistically significant...
... time point of 60 in order to measure accurately the synergistic effect EGF concentration-dependency ofsynergisticactivation To investigate the synergisticactivation in the second phase of the ... first peak, the ERK-PP concentration reaches a quasisteady state-concentration of 2–3· the sum of the concentration obtained after h of stimulation with only EGF and the concentration obtained ... the sum of the ERK-PP concentrations after stimulation with EGF and PMA separately on the other hand, was not constant, but increased with the EGF concentration, which confirmed synergistic activation...
... third activation domain, AD3, was recently identified in the highly conserved N-terminal bHLH-PAS domain of p160 co- activators by recruitment of secondary co- activators, including coiled-coil co- activator ... co- activator (CoCoA) and GRIP1-associated co- activator 63 (GAC63) [29,30] As CoCoA and GAC63 have no obvious sequence homology, the nature of their downstream targets and the specific components of the ... region of GRIP1 is involved in the repression of transactivation activity and selfassociation of GRIP1 (Figs 1–5), we examined the relationship between transactivation and co- activationof GRIP1,...
... previous study [7] who measured a Kd of 0.2 lM, may be attributable to conformational differences in the site in the context of G4–6 compared to G5–6 or a degree of cocooperativity between calcium site ... due to coordinationof calcium sites as is proposed for IIG2 and IIG6, in addition to the general opening of the molecule Cooperation of the sites would also explain why the dissociation of G2 ... a function of calcium concentration (Fig insert) A simple reduction of fluorescence was observed with half maximum change occurring at a calcium concentration of 15 lM Interaction of G2 segment...
... Figure Activationof NADPH oxidase in the presence of glial iNOS is synergistic in killing co- cultured neurons Activationof NADPH oxidase in the presence of glial iNOS is synergistic in killing co- cultured ... proliferation of glia) consisting of 97 ± 4% neurons, ± Page of 15 (page number not for citation purposes) Journal of Neuroinflammation 2005, 2:20 http://www.jneuroinflammation.com/content/2/1/20 ... oxidase to produce superoxide is synergistic with NO from iNOS in inducing death of co- cultured neurons, whereas activationof either alone causes little or no death of co- cultured neurons Materials...
... Effect of cholesterol on quercetin incorporation Effect of incorporation of mol% of DSPE-PEG 0 on the incorporation of quercetin 50 4.2.3 Influence of different lipids on quercetin incorporation ... inhibition of the PI3KAKT/PKB pathway (Gulati, Laudet et al 2006), downregulation of the expression of oncogenes and anti-oncogenes (Ranelletti, Maggiano et al 2000), upregulation of cell cycle control ... generation of liposomes The second generation of liposomes consists of sterically stabilized liposomes where surface grafted hydrophilic polymers such as poly(ethylene glycol) are incorporated...
... 22:00), and commencement and completion of the daily medical shift (08:00–18:00) Statistical analysis The frequency of MET service activation during peak periods was compared with the average activation ... have no competing interests Authors' contributions DJ conceived the study, constructed the data base, and was the principle author of the manuscript SB, DG, and SW assisted with construction of the ... frequent activationof the MET service in the evening In a study of 522 MET calls over a 1-year period, Lee and coworkers [5] demonstrated that 36% of MET calls were Figure 18:00–08:00 comparison...
... preferentially the phosphorylation of the p46 isoform of JNK, whereas sorbitol treatment induced phosphorylation of both the p46 and p55 isoforms To confirm functional activationof the JNK signaling pathway, ... subcellular localization of JSP1, we asked whether abrogation of myristoylation would also affect JSP1-induced JNK activation We transfected Cos-1 cells with expression constructs encoding JSP1-wt or ... as the control Cells detached from the culture dish were collected, stained with Trypan Blue and counted The results are the mean of three experiments (±standard error of the mean) (C) Cos-1 cells...
... Journal compilation ª 2010 FEBS 3161 Role of GRASP55 in MT1-MMP activation C Roghi et al Fig Co- localization of MT1-MMP and GRASP55 in live cells Four consecutive frames (4 s apart) of time lapse ... Journal compilation ª 2010 FEBS C Roghi et al Role of GRASP55 in MT1-MMP activation ting that the disruption of the interaction between MT1-MMP and GRASP55 could affect the activationof the protease ... Authors Journal compilation ª 2010 FEBS 3159 Role of GRASP55 in MT1-MMP activation C Roghi et al to influence the O-glycosylation pattern of MT1-MMP [29] Post-translational modifications of the MT1MMP...
... LPS; this might reflect a loss of cell viability or numbers at the high LPS concentration Time-course experiments were performed at the concentration of lgÆmL)1 (Fig 4B) Conspicuous ICAM-1 biosynthesis ... time course of ICAM-1 expression after LPS injection is shown in Fig 1B The expression pattern for ICAM-1 protein was similar to that for ICAM-1 mRNA Compared with the control, expression of ICAM-1 ... in Schwann cells of sciatic nerves Effects of LPS on expression of ICAM-1 in SCs in vitro In order to better explore the role of LPS-induced ICAM-1 expression in SCs, a series of experiments were...
... effects are presented as n-fold activationof the respective TIMP-1 promoter constructs in comparison to the effects of empty vector (control) All length variants of the human TIMP-1 promoter proved ... transcriptional upregulation of TIMP-1 We show that activationof Rac induces ROS formation, followed by ERK1,2 activation and AP-1-mediated TIMP-1 promoter activation In contrast, other signaling ... sustained activationof Rac by overexpression of the Rac-specific activator, Tiam1, or overexpression of V12-Rac1, strongly induces transcriptional upregulation of tissue inhibitor of metalloproteinase...
... Identification of tryptophan 55 as the primary site of [3H]nicotine photoincorporation in the c-subunit of the Torpedo nicotinic acetylcholine receptor FEBS Lett 423, 223–226 17 Xie Y & Cohen JB (2001) Contributions ... the concentration of binding sites in terms of nAÆfmol)1 For competition curves, oocytes were incubated for 40 with various concentrations of ACh in a 96-well plate prior to the addition of 2.5 ... presence of inhibitor at concentration [X], IC50 is the inhibitor concentration that reduced the amplitude of AChevoked current by 50% and n is the Hill coefficient ACh inhibition of initial rate of...
... instead of using all words Our goal is compare the sentiment lexicon constructed by the RAP method with other general lexicons on the impact of for sentiment classification The general lexicons used ... of text and aspect ratings for sentiment summarization In Proceedings of the 46th Annual Meeting of the Association of Computational Linguistics: Human Language Technologies, pages 308–316, Columbus, ... language Comput Linguist., 30:277–308, Sept Theresa Wilson, Janyce Wiebe, and Paul Hoffmann 2005 Recognizing contextual polarity in phrase-level sentiment analysis In Proceedings of the conference...
... sites of pcDNA3 (Invitrogen) under the control of the CMV promoter PEGFP-N3 plasmid (Clontech) expressing GFP under the control of the CMV promoter was used as control plasmid for the cotransfection ... pGL3-basic (Promega) as the second cistron A 22-base pair fragment containing StuI and NcoI sites separates the stop codon of the Renilla luciferase and the ATG initiator codon of the firefly luciferase ... (Invitrogen) under the control of the CMV promoter pcPKRD6 ¼ p68D6-pcDNAI/NEO [19] was used for expression of PKRD6, the dominant-negative variant of PKR under the control of the CMV promoter pc2a-S51A...
... in the concentration range of 30–150 lM (1.3–6.5 mgÆmL)1), 90 lL of arrestin solution was mixed with 10 lL of 10 mM peptide solution to yield a final peptide concentration of mM For higher concentrations ... proportional to the product of the molecular mass and concentration of the scattering particle For a mixture of particles, the total forward scattering, I(0)total is equal to the sum of contributions from ... equilibrium dissociation constant, Kd, of approximately 100 lM (corresponding to 4–5 mgÆmL)1) In contrast to a previous study [14], there does not seem to be a significant concentration of tetrameric arrestin,...
... activity (A) Membranes of COS cells expressing ANF-RGC were incubated with 10)7 M ANF in the presence of the indicated concentrations of staurosporine, AMP-PNP or ATP (B) Membranes of COS cells expressing ... nonhydrolyzable analog of ATP, AMP-PNP mimicked 60–70% of the ATP effect with respect to ANF activationof ANF-RGC activity [22,25] The remaining 40–30% of ATP activity in ANF-RGC activation was predicted ... approximately 85% of [c32P]-8-azido-ATP (Fig 1C) [27] The fact that a concentration of staurosporine almost one order of magnitude lower than that of ATP is needed to displace half of the bound [c32P]-8-azidoATP...
... (a) the competitive effect of AHA on collagen-induced PMN activation, (b) the effect of the carbamylation of lysine side chains on collageninduced PMN activation and (c) the effect of a recombinant ... 0.01) when compared with control peptides and taking into account the basal activation state of PMNs Discussion PMNs interact with various types of collagen in vivo, especially with type I collagen, ... peptides of pepsinized collagen, deprived of telopeptides, were used as a negative control of PMN activation (A) Coomassie Brilliant Blue-stained CNBr peptides separated by electrophoresis (B) Activation...
... engineered EcoRI recognition site is single-underlined The start codon is double-underlined The modified original start codon of M2 is dot-underlined The engineered region containing mycepitope tag encoded ... indicating that the side effect of the activationof GAR-3 is limited Furthermore, the phenotype of gar-3 loss -of- function mutants is almost wildtype with the exception of a faster pharyngeal pumping ... C-terminus of M2 A myc-epitope tag was added at the N-terminus of M2 To prevent rapid degradation, the central part of the third intracellular loop of M2 was deleted [26] Asn at putative N-glycosylation...
... 6.5% (P < 0.01), compared with cells completely deprived of serum The activity of neokyotorphin was evaluated compared with the corresponding controls In contrast to fetal bovine serumdeficient ... 7) The activity of lm neokyotorphin was inhibited over the whole concentration range of H-89, confirming the involvement of PKA in the effect of neokyotorphin As for participation of the other H-89-suppressed ... number of cells in one well of a 96-well assay plate was calculated as: Nwell ¼ Ncounted · 10, where Nwell is the number of cells in a well and Ncounted is cell count returned by the counter...
... cells via activationof NF-jB Attenuation of LXF-289 cell proliferation by ATP is mediated by the activationof the MEK ⁄ ERK1 ⁄ 2, PI3K and p38 MAPK pathways Activation and translocation of the ... Inhibition of proliferation of LXF-289 lung tumor cells Effects of P2 receptor agonists The influence of the different P2 receptor agonists is shown as percentage inhibition of the proliferation of LXF-289 ... influx, are involved in the control of LXF-289 cell proliferation by P2Y receptors At a concentration of 10 lm, the PLC inhibitor U-73122 completely reversed the effect of ATP on FEBS Journal 273...