coordination abnormal co activation of synergistic muscles
Báo cáo khoa học: Synergistic co-operation of signal transducer and activator of transcription 5B with activator protein 1 in angiotensin II-induced angiotensinogen gene activation in vascular smooth muscle cells potx
... the transactivation transcription of the AGT gene in VSMCs Super-EMSA and ChIP confirmed that Ang II could increase the binding activity of STAT5B to the ciselement and the recruitment of STAT5B ... Definitive evidence of physical association between c-Jun and STAT5B is provided by the results of the CoIP and super-EMSA of nuclear extracts, and by the results of the ChIP assay of immunoprecipitated ... means ± SD, and an analysis of variance with Bonferroni’s test was used for the statistical analysis of multiple comparisons of data P-values of less than 0.05 were considered statistically significant...
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Tài liệu Báo cáo khoa học: Synergistic activation of signalling to extracellular signal-regulated kinases 1 and 2 by epidermal growth factor and 4b-phorbol 12-myristate 13-acetate pptx
... time point of 60 in order to measure accurately the synergistic effect EGF concentration-dependency of synergistic activation To investigate the synergistic activation in the second phase of the ... first peak, the ERK-PP concentration reaches a quasisteady state-concentration of 2–3· the sum of the concentration obtained after h of stimulation with only EGF and the concentration obtained ... the sum of the ERK-PP concentrations after stimulation with EGF and PMA separately on the other hand, was not constant, but increased with the EGF concentration, which confirmed synergistic activation...
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Báo cáo khoa học: Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains pptx
... third activation domain, AD3, was recently identified in the highly conserved N-terminal bHLH-PAS domain of p160 co- activators by recruitment of secondary co- activators, including coiled-coil co- activator ... co- activator (CoCoA) and GRIP1-associated co- activator 63 (GAC63) [29,30] As CoCoA and GAC63 have no obvious sequence homology, the nature of their downstream targets and the specific components of the ... region of GRIP1 is involved in the repression of transactivation activity and selfassociation of GRIP1 (Figs 1–5), we examined the relationship between transactivation and co- activation of GRIP1,...
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Báo cáo khoa học: Co-operation of domain-binding and calcium-binding sites in the activation of gelsolin pptx
... previous study [7] who measured a Kd of 0.2 lM, may be attributable to conformational differences in the site in the context of G4–6 compared to G5–6 or a degree of cocooperativity between calcium site ... due to coordination of calcium sites as is proposed for IIG2 and IIG6, in addition to the general opening of the molecule Cooperation of the sites would also explain why the dissociation of G2 ... a function of calcium concentration (Fig insert) A simple reduction of fluorescence was observed with half maximum change occurring at a calcium concentration of 15 lM Interaction of G2 segment...
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báo cáo hóa học: " Activation of microglial NADPH oxidase is synergistic with glial iNOS expression in inducing neuronal death: a dual-key mechanism of inflammatory neurodegeneration" docx
... Figure Activation of NADPH oxidase in the presence of glial iNOS is synergistic in killing co- cultured neurons Activation of NADPH oxidase in the presence of glial iNOS is synergistic in killing co- cultured ... proliferation of glia) consisting of 97 ± 4% neurons, ± Page of 15 (page number not for citation purposes) Journal of Neuroinflammation 2005, 2:20 http://www.jneuroinflammation.com/content/2/1/20 ... oxidase to produce superoxide is synergistic with NO from iNOS in inducing death of co- cultured neurons, whereas activation of either alone causes little or no death of co- cultured neurons Materials...
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Liposomal co encapsulation of quercetin with synergistic chemotherapeutic drugs for breast cancer treatment
... Effect of cholesterol on quercetin incorporation Effect of incorporation of mol% of DSPE-PEG 0 on the incorporation of quercetin 50 4.2.3 Influence of different lipids on quercetin incorporation ... inhibition of the PI3KAKT/PKB pathway (Gulati, Laudet et al 2006), downregulation of the expression of oncogenes and anti-oncogenes (Ranelletti, Maggiano et al 2000), upregulation of cell cycle control ... generation of liposomes The second generation of liposomes consists of sterically stabilized liposomes where surface grafted hydrophilic polymers such as poly(ethylene glycol) are incorporated...
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Báo cáo khoa học: "Circadian pattern of activation of the medical emergency team in a teaching hospita"
... 22:00), and commencement and completion of the daily medical shift (08:00–18:00) Statistical analysis The frequency of MET service activation during peak periods was compared with the average activation ... have no competing interests Authors' contributions DJ conceived the study, constructed the data base, and was the principle author of the manuscript SB, DG, and SW assisted with construction of the ... frequent activation of the MET service in the evening In a study of 522 MET calls over a 1-year period, Lee and coworkers [5] demonstrated that 36% of MET calls were Figure 18:00–08:00 comparison...
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Tài liệu Báo cáo khoa học: Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf
... preferentially the phosphorylation of the p46 isoform of JNK, whereas sorbitol treatment induced phosphorylation of both the p46 and p55 isoforms To confirm functional activation of the JNK signaling pathway, ... subcellular localization of JSP1, we asked whether abrogation of myristoylation would also affect JSP1-induced JNK activation We transfected Cos-1 cells with expression constructs encoding JSP1-wt or ... as the control Cells detached from the culture dish were collected, stained with Trypan Blue and counted The results are the mean of three experiments (±standard error of the mean) (C) Cos-1 cells...
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Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf
... Journal compilation ª 2010 FEBS 3161 Role of GRASP55 in MT1-MMP activation C Roghi et al Fig Co- localization of MT1-MMP and GRASP55 in live cells Four consecutive frames (4 s apart) of time lapse ... Journal compilation ª 2010 FEBS C Roghi et al Role of GRASP55 in MT1-MMP activation ting that the disruption of the interaction between MT1-MMP and GRASP55 could affect the activation of the protease ... Authors Journal compilation ª 2010 FEBS 3159 Role of GRASP55 in MT1-MMP activation C Roghi et al to influence the O-glycosylation pattern of MT1-MMP [29] Post-translational modifications of the MT1MMP...
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Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt
... LPS; this might reflect a loss of cell viability or numbers at the high LPS concentration Time-course experiments were performed at the concentration of lgÆmL)1 (Fig 4B) Conspicuous ICAM-1 biosynthesis ... time course of ICAM-1 expression after LPS injection is shown in Fig 1B The expression pattern for ICAM-1 protein was similar to that for ICAM-1 mRNA Compared with the control, expression of ICAM-1 ... in Schwann cells of sciatic nerves Effects of LPS on expression of ICAM-1 in SCs in vitro In order to better explore the role of LPS-induced ICAM-1 expression in SCs, a series of experiments were...
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Tài liệu Báo cáo khoa học: Rac upregulates tissue inhibitor of metalloproteinase-1 expression by redox-dependent activation of extracellular signal-regulated kinase signaling pptx
... effects are presented as n-fold activation of the respective TIMP-1 promoter constructs in comparison to the effects of empty vector (control) All length variants of the human TIMP-1 promoter proved ... transcriptional upregulation of TIMP-1 We show that activation of Rac induces ROS formation, followed by ERK1,2 activation and AP-1-mediated TIMP-1 promoter activation In contrast, other signaling ... sustained activation of Rac by overexpression of the Rac-specific activator, Tiam1, or overexpression of V12-Rac1, strongly induces transcriptional upregulation of tissue inhibitor of metalloproteinase...
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Tài liệu Báo cáo khoa học: Activation of the Torpedo nicotinic acetylcholine receptor The contribution of residues aArg55 and cGlu93 ppt
... Identification of tryptophan 55 as the primary site of [3H]nicotine photoincorporation in the c-subunit of the Torpedo nicotinic acetylcholine receptor FEBS Lett 423, 223–226 17 Xie Y & Cohen JB (2001) Contributions ... the concentration of binding sites in terms of nAÆfmol)1 For competition curves, oocytes were incubated for 40 with various concentrations of ACh in a 96-well plate prior to the addition of 2.5 ... presence of inhibitor at concentration [X], IC50 is the inhibitor concentration that reduced the amplitude of AChevoked current by 50% and n is the Hill coefficient ACh inhibition of initial rate of...
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Tài liệu Báo cáo khoa học: "Cross-Domain Co-Extraction of Sentiment and Topic Lexicons" pdf
... instead of using all words Our goal is compare the sentiment lexicon constructed by the RAP method with other general lexicons on the impact of for sentiment classification The general lexicons used ... of text and aspect ratings for sentiment summarization In Proceedings of the 46th Annual Meeting of the Association of Computational Linguistics: Human Language Technologies, pages 308–316, Columbus, ... language Comput Linguist., 30:277–308, Sept Theresa Wilson, Janyce Wiebe, and Paul Hoffmann 2005 Recognizing contextual polarity in phrase-level sentiment analysis In Proceedings of the conference...
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Tài liệu Báo cáo Y học: Phosphorylation of initiation factor-2a is required for activation of internal translation initiation during cell differentiation ppt
... sites of pcDNA3 (Invitrogen) under the control of the CMV promoter PEGFP-N3 plasmid (Clontech) expressing GFP under the control of the CMV promoter was used as control plasmid for the cotransfection ... pGL3-basic (Promega) as the second cistron A 22-base pair fragment containing StuI and NcoI sites separates the stop codon of the Renilla luciferase and the ATG initiator codon of the firefly luciferase ... (Invitrogen) under the control of the CMV promoter pcPKRD6 ¼ p68D6-pcDNAI/NEO [19] was used for expression of PKRD6, the dominant-negative variant of PKR under the control of the CMV promoter pc2a-S51A...
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Tài liệu Báo cáo Y học: The solution structure and activation of visual arrestin studied by small-angle X-ray scattering pot
... in the concentration range of 30–150 lM (1.3–6.5 mgÆmL)1), 90 lL of arrestin solution was mixed with 10 lL of 10 mM peptide solution to yield a final peptide concentration of mM For higher concentrations ... proportional to the product of the molecular mass and concentration of the scattering particle For a mixture of particles, the total forward scattering, I(0)total is equal to the sum of contributions from ... equilibrium dissociation constant, Kd, of approximately 100 lM (corresponding to 4–5 mgÆmL)1) In contrast to a previous study [14], there does not seem to be a significant concentration of tetrameric arrestin,...
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Báo cáo khoa học: ATP allosteric activation of atrial natriuretic factor receptor guanylate cyclase pot
... activity (A) Membranes of COS cells expressing ANF-RGC were incubated with 10)7 M ANF in the presence of the indicated concentrations of staurosporine, AMP-PNP or ATP (B) Membranes of COS cells expressing ... nonhydrolyzable analog of ATP, AMP-PNP mimicked 60–70% of the ATP effect with respect to ANF activation of ANF-RGC activity [22,25] The remaining 40–30% of ATP activity in ANF-RGC activation was predicted ... approximately 85% of [c32P]-8-azido-ATP (Fig 1C) [27] The fact that a concentration of staurosporine almost one order of magnitude lower than that of ATP is needed to displace half of the bound [c32P]-8-azidoATP...
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Báo cáo khoa học: Involvement of lysine 1047 in type I collagen-mediated activation of polymorphonuclear neutrophils doc
... (a) the competitive effect of AHA on collagen-induced PMN activation, (b) the effect of the carbamylation of lysine side chains on collageninduced PMN activation and (c) the effect of a recombinant ... 0.01) when compared with control peptides and taking into account the basal activation state of PMNs Discussion PMNs interact with various types of collagen in vivo, especially with type I collagen, ... peptides of pepsinized collagen, deprived of telopeptides, were used as a negative control of PMN activation (A) Coomassie Brilliant Blue-stained CNBr peptides separated by electrophoresis (B) Activation...
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Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc
... engineered EcoRI recognition site is single-underlined The start codon is double-underlined The modified original start codon of M2 is dot-underlined The engineered region containing mycepitope tag encoded ... indicating that the side effect of the activation of GAR-3 is limited Furthermore, the phenotype of gar-3 loss -of- function mutants is almost wildtype with the exception of a faster pharyngeal pumping ... C-terminus of M2 A myc-epitope tag was added at the N-terminus of M2 To prevent rapid degradation, the central part of the third intracellular loop of M2 was deleted [26] Asn at putative N-glycosylation...
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Báo cáo khoa học: Stimulation of fibroblast proliferation by neokyotorphin requires Ca2+ influx and activation of PKA, CaMK II and MAPK/ERK pdf
... 6.5% (P < 0.01), compared with cells completely deprived of serum The activity of neokyotorphin was evaluated compared with the corresponding controls In contrast to fetal bovine serumdeficient ... 7) The activity of lm neokyotorphin was inhibited over the whole concentration range of H-89, confirming the involvement of PKA in the effect of neokyotorphin As for participation of the other H-89-suppressed ... number of cells in one well of a 96-well assay plate was calculated as: Nwell ¼ Ncounted · 10, where Nwell is the number of cells in a well and Ncounted is cell count returned by the counter...
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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc
... cells via activation of NF-jB Attenuation of LXF-289 cell proliferation by ATP is mediated by the activation of the MEK ⁄ ERK1 ⁄ 2, PI3K and p38 MAPK pathways Activation and translocation of the ... Inhibition of proliferation of LXF-289 lung tumor cells Effects of P2 receptor agonists The influence of the different P2 receptor agonists is shown as percentage inhibition of the proliferation of LXF-289 ... influx, are involved in the control of LXF-289 cell proliferation by P2Y receptors At a concentration of 10 lm, the PLC inhibitor U-73122 completely reversed the effect of ATP on FEBS Journal 273...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25