conversion of human es cells to neural cells in adherent monolayer
báo cáo khoa học: " In vitro proliferation of human osteogenic cells in presence of different commercial bone substitute materials combined with enamel matrix derivatives" pdf
... that an increasing number of interconnective pores raises the internal surface area of a BSM, with promotion of the growth of regenerative cells [33] The assessment of cell proliferation in vitro ... proteins, and initiation of cementogenesis in porcine teeth J Clin Periodontol 2004, 31(8):184-192 Guida L, et al.: In vitro biologic response of human bone marrow stromal cells to enamel matrix derivative ... [21] Altogether, the addition of EMD seems to promote osteogenic cell proliferation to a certain degree In the routine clinical situation, the benefit of combining BSM and EMD is well established,...
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Báo cáo y học: "CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model" pdf
... with integrins, whereas the cytoplasmic tail may be involved in regulation of the cytoskeleton through PLICs (proteins linking IAP to cytoskeleton) [32,33] Alternatively, inter- Available online ... cell line, rather than human chondrocytes, in an attempt to provide additional understanding of CD47/IAP involvement in cellular mechanotransduction Although these cells may express integrin profiles ... modulate integrin affinity without intracellular signalling has been suggested [31] The extracellular IgV domain of CD47/IAP may interact with the integrin, inducing change to a high-affinity state...
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Báo cáo y học: " Many LINE1 elements contribute to the transcriptome of human somatic cells" pptx
... LINE-1 sequences in human cells producing a LINE-1encoded protein Gene 1993, 133:273-277 Yu F, Zingler N, Schumann G, Stratling WH: Methyl-CpG-binding protein represses LINE-1 expression and retrotransposition ... influenced by the presence of these retroelements Conclusions We have identified expressed sequence tags corresponding to 692 distinct L1 element sites in human lymphoblastoid cells, indicating that retrotransposon-derived ... encompassing 1,592 sequences corresponding to 271 unique sites Of these sites, 228 corresponded to an L1 element in the reference genome The remaining 43 clusters, while containing L1 3' UTR sequence...
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Báo cáo y học: "TLR2 and TLR4 triggering exerts contrasting effects with regard to HIV-1 infection of human dendritic cells and subsequent virus transfer to CD4+ T cells" docx
... these cytokines induce the secretion of other proinflammatory cytokines or recruit other immune cells, thus possibly increasing the number of Page 11 of 16 (page number not for citation purposes) ... long enough to obtain DC maturation but sufficient to trigger some biological responses such as cytoskeleton remodelling and an increase in macropinocytosis [62] Interestingly, the shape of LPS-stimulated ... migrate to the most proximal lymph nodes to prime CD4+ T cells The recognition of PAMPs by TLRs triggers intracellular signalling pathways, which culminate in secretion of proinflammatory cytokines,...
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Analysis of the mechanical reaction of human endothelial cells to a single constant or transient uniaxial strain
... blood presses on the walls of veins and shears the endothelium when flowing Furthermore, the cells are exposed to stresses applied by adjacent tissues While forming an inner lining of blood vessels, ... conditions of the environment Single actin filaments connected by cross-linking proteins like α-actinin form actin bundles Several bundles assemble into more stable and thicker stress-fibres linked ... mechanosensors, for instance, by inhibiting binding of fiber severing proteins as cofilin under strain, or by means of focal adhesion associated proteins, like zyxin and talin [24], [25] Figure...
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Báo cáo khoa học: Differential post-translational modification of CD63 molecules during maturation of human dendritic cells potx
... component of Weibel-Palade bodies of human endothelial cells Blood 82, 1184–1191 53 Tapper, H & Grinstein, S (1997) Fc receptor-triggered insertion of secretory granules into the plasma membrane of human ... carbohydrate residues Interestingly, after maturation of dendritic cells, the apparent molecular mass of each of these eight carbohydrate containing polypeptides was increased by 10 kDa (Fig 2B) In mature ... N-lactosamine groups are sensitive to endo-b-galactosidase [43] To analyze the presence of polylactosaminoglycans, immature dendritic cells and maturing cells, cultured for 12 h in the presence of...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... vaccinated nine times and immunological responses to the autologous vaccination were investigated We first assessed the ability of autologous FCs vaccination to stimulate T cells After the ninth ... responses Immature DCs are one of the mediators of tolerance induction In peripheral lymphoid organs immature DCs are incapable of mobilizing CTL responses and have been reported to induce tolerance ... activators of innate immune responses, inducing DCs maturation and inflammatory cytokine secretion by innate immune cells and as a consequence they promote antitumor immune responses when coadministered...
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báo cáo hóa học:" Vasoprotective effects of human CD34+ cells: towards clinical applications" pptx
... pre-contraction with phenylephrine in an organ chamber, relaxation in response to incremental doses of acetylcholine was assessed (Figure 3) Maximal relaxation of vessel rings from human CD34+ treated animals ... BMendothelial lineage cells express angiogenic ligands and cytokines [14,15] and induce proliferation of preexisting vasculature in the vicinity of myocardial infarcts [16] The margin by which CD34+ ... expressed CD133 and less than 1% of CD34+ cells were positive for VEGFR2 (Figure 1) Tracking of delivered human CD34+ cells To determine whether delivery of cells resulted in any cell retention...
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báo cáo hóa học:" Activation of human B cells by the agonist CD40 antibody CP-870,893 and augmentation with simultaneous toll-like receptor 9 stimulation" doc
... proliferation of T cells that secrete high levels of effector cytokines, suggesting a potential role for CP-873,893 in licensing CD40-expressing APC in humans to enable high quality T cell responses Third, ... B cells and CD27+ memory B cells Cytokine secretion by in vitro stimulated B cells To determine whether CP-870,893 induces human B cells to produce cytokines, supernatant from stimulated B cells ... CP870,893 activates human B cells, including classically defined memory and naïve subsets, triggering increased expression of immuno-stimulatory molecules and production of cytokines Furthermore,...
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Báo cáo hóa học: "Future research and therapeutic applications of human stem cells: general, regulatory, and bioethical aspects" ppt
... T cells and inhibiting production of cytotoxic T cells, NK cells, and proinflammatory cytokines such as tumor necrosis factor-a (TNF-a), interferon-g (IFN-g), and interleukine-12 (IL-12) These ... in the presence of adequate factors–as occurs in mesenchymal stem cells, which are able to differentiate into cells of an ectodermal (neurons and skin) and endodermal (hepatocytes, lung and intestinal ... stem cells in human brain [41] The high renewal capacity of the skin is due to the presence in the epidermis of stem cells acting as a cell reservoir These include epidermal stem cells, mainly...
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Báo cáo sinh học: " Transmission of human hepatitis C virus from patients in secondary cells for long term culture" pot
... reported by many investigators using a large variety of established cell lines comprising of various cell types [16] These included human transformed liver cells in addition to Hela, CEM, H9, ... RNA for HCV in a few B -cells, liver cells, and monocytoid cells, none of these standard cell lines produced infectious HCV that could be transmitted into uninfected cells These freshly infected ... liver biopsies Kupffer's cells are liver macrophages and Hepatocytes are liver endothelial cells 2B -cells human foreskin fibroblast (ATCC, Bethesda, MD) All of these cell types could be infected...
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Báo cáo sinh học: " Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot
... NK cells are dispensable for the innate resistance of mice to HSV-1 infection, further investigation is necessary to determine what role, if any, NK cells play in human resistance to HSV-1 infection ... suggests that IFN-α/β and IFN-γ are important activators of Stat 1-induced resistance to HSV-1 in vivo However, further studies are necessary to determine if chemokines and pro-inflammatory cytokines ... Role of NK cells in innate resistance to HSV-1 Despite a large differential in NK cell cytotoxicity, NOD scid mice possessed an innate resistance to HSV-1 infection that was equivalent to BALB/c...
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báo cáo hóa học:" Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot
... NK cells are dispensable for the innate resistance of mice to HSV-1 infection, further investigation is necessary to determine what role, if any, NK cells play in human resistance to HSV-1 infection ... suggests that IFN-α/β and IFN-γ are important activators of Stat 1-induced resistance to HSV-1 in vivo However, further studies are necessary to determine if chemokines and pro-inflammatory cytokines ... Role of NK cells in innate resistance to HSV-1 Despite a large differential in NK cell cytotoxicity, NOD scid mice possessed an innate resistance to HSV-1 infection that was equivalent to BALB/c...
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Báo cáo hóa học: " Novel Strategy to Fabricate PLA/Au Nanocomposites as an Efficient Drug Carrier for Human Leukemia Cells in Vitro" pdf
... K562/AO2 cells than that of K562 cells, as shown in Figure 4a and 4b Interestingly, Page of the presence of PLA/Au nanocomposites could efficiently facilitate the drug uptake of DNR into the drug-resistant ... penicillin (100 mU/mL) and streptomycin (100 μg/mL) at 37°C in a humidified atmosphere containing 5% CO2, while μg/mL doxorubicin was contained in the culture to maintain K562/AO2 cells drug resistance ... leukemia cells and lead to the much higher intracellular drug concentration in the target cells, resulting in the remarkable enhancement of the intracellular fluorescence of drug-resistant leukemia cells...
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Báo cáo y học: "Decreased response to IL-12 and IL-18 of peripheral blood cells in rheumatoid arthritis" doc
... the response to Th1-inducing cytokines in RA These results indicate that RA-PBMCs are defective in their response to Th1-inducing cytokines, with an additional effect related to age When looking ... demonstrated the presence of IL-12 as a contributory factor in inducing the IFN-γ-dominant T-cell cytokine response in joints of patients with longstanding RA [13] IL-18 is a proinflammatory cytokine that ... production of inflammatory cytokines such as TNF-α [20] To control some of the potentially deleterious properties of IL-18, IL-18-binding protein (IL-18BP) has been identified as a specific inhibitor of...
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báo cáo khoa học: "Mitochondrial and endoplasmic reticulum stress pathways cooperate in zearalenone-induced apoptosis of human leukemic cells" doc
... lines, but less in PBMCs, as evidenced by presence of apoptotic bodies and cells with subdiploid peaks (representing DNA fragmentation) ZEA is cytotoxic to bovine lymphocytes [40] and induces ... levels were increased in cytosol and mitochondria, suggesting the involvement of ER stress in ZEA-treated human leukemic cells 2DPAGE of HL-60 treated cells showed increased expression of GRP78, ... 2) These results point to the presence of ER stress in ZEA-treated leukemic cells ER stress gene expression at mRNA levels Figure Expression of Bax, Bcl-2 and Bcl-xL in ZEA-treated HL60 cells...
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Báo cáo y học: "Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage" potx
... immunofluorescence, analyzing the zonal distribution of these cells within the matrix of normal and OA cartilage was of particular interest Owing to the inherent autofluorescence properties of cartilage matrix ... pronounced in CD166+ cells, indicating more progenitor cells in CD166+ than CD166- cells (Figure 5c,d) As for chondrogenesis, only the pellet cultures of CD166 + cells showed a clear deposition of extracellular ... [17] Besides being expressed on thymic epithelial cells, activated T cells, Blymphocytes, and monocytes, CD166 is expressed on a subpopulation of human cartilage cells [5,18] Even though the presence...
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Báo cáo y học: "Construction of predictive promoter models on the example of antibacterial response of human epithelial cells" pdf
... examining the ways in which the response of the eukaryotic cell(s) is triggered, leading us to a better understanding of the details of the inflammatory process in general After adhesion of P ... values for m, n, i and r1, r2 Also, we are interested only in pairs, which are present in at least a minimum fraction of (+)training sequences (C1) and in a defined maximum fraction of (-)-training ... combinations of transcription factor binding sites according to mutual orientation, we allow inversions of the whole module This gives rise to a total of three classes as shown Identification of...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... antibody From these experiments, we obtained 16 murine ESC lines from 164 source blastocysts Assessments via alkaline phosphatase and Oct-4 to verify pluripotency of the ESC lines were in agreement ... benefit the lives of millions of people who suffer from life destroying diseases Only certain cell lines will be considered eligible for federally-sponsored human ESC research These cell lines must ... monitor quality in such cells are also needed to bring the full range of therapeutic possibility into focus In an effort to develop an assessment system for ESCs, our center cultured murine blastocysts...
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