control of rem sleep and waking related activities
Tài liệu Báo cáo khoa học: Antimicrobial effects of H4-(86–100), histogranin and related compounds – possible involvement of DNA gyrase ppt
... bactericidal potencies of H4-(86–100), HNr and related peptides and nonpeptides in E coli were compared with those of the cationic peptides protegrin and LL-37 (Table 2) Among the various HN -related FEBS ... 3E,F) Time dependence of the bactericidal activity of H4-(86–100) and ciprofloxacin In order to evaluate the rapidity of action of histone H4 peptides and compare it with that of the quinolone antibiotic ... activities of H4-(86–100) and some related compounds were also assessed by the radial diffusion assay (Fig 5) In this test, H4-(86–100) was equipotent with HNr and compound 3, and about 4.2 and...
- 12
- 756
- 0
Báo cáo khoa học: Possible involvement of an FKBP family member protein from a psychrotrophic bacterium Shewanella sp. SIB1 in cold-adaptation potx
... represents the concentration of the enzyme, and kp and kn represent the first-order rate constants for the release of p-nitroaniline from the substrate in the presence and absence of the enzyme, respectively ... where kp and kn represent the firstorder rate constants for the faster refolding phase of RNase T1 in the presence and absence of the enzyme, respectively Results and discussion Detection of a protein ... htpX-like and dapB-like genes that are located 79 bp upstream and 84 bp downstream of the Sh-fklB gene (Fig 2) The htpX-like and dapB-like genes encode a homologue of a zinc protease and a dihydrodipicolinate...
- 10
- 436
- 0
Báo cáo y học: "Expression and function of inducible co-stimulator in patients with systemic lupus erythematosus: possible involvement in excessive interferon-γ and anti-double-stranded DNA antibody production" pot
... staining and flow cytometry for ICOS expression Percentages of ICOS+ cells (panels c and d) and MFIs of ICOS+ cells (panels e and f) are shown CD4+CD45RO+ (panels c and e) and CD8+CD45RO+ (panels d and ... (Miltenyi Biotech) and anti-CD14 MicroBeads (Miltenyi Biotech) The purities of B cells and T cells were in excess of 97% and 95%, respectively, using flow cytometry Immunoprecipitation and Western blotting ... to human ICOS and had greater avidity than SA12 We also compared the binding profiles of SA12 and JTA009 to peripheral blood T cells from 11 normal control individuals Percentages of cells positive...
- 14
- 492
- 0
Báo cáo y học: " Involvement of the genicular branches in cystic adventitial disease of the popliteal artery as a possible marker of unfavourable early clinical outcome: a case report" pps
... contents of incised cyst on the posterior wall of the popliteal artery Because of the rarity of the disease and the lack of large studies involving long follow-up examinations, the recurrence rate of ... extension of CAD to the genicular arteries could be a predictor of higher risk of recurrence, either as an indicator of cysts communicating with the knee synovium or as a marker of the involvement of ... employed in the management of the disease Despite reports of spontaneous resolution of symptoms [9], the majority of patients require surgery Intravascular angioplasty and stenting have been described...
- 4
- 333
- 0
ISOTOPE HYDROGRAPH SEPARATION FOR MODELING OF RUNOFF MECHANISMS OF ATMOSPHERICALLY DERIVED CHEMICAL AND RADIOACTIVE POLLUTANTS
... composition of 2H and 18O The compositions of the isotopes 2H (D) and 18O were analyzed to obtain δD (‰) and δ18O (‰), respectively, which are defined as the relative deviation of the parts per thousand ... allowances related to the field work by the authorities of the Fukushima Prefecture and the Ministry of Land, Infrastructure and Transport are appreciated The authors thank Dr Mariko Atarashi-Andoh ... the discharge of new water and the migration of hazardous elements, which has not been sufficiently analyzed or reported previously As a result of the calculation, and by setting C1 and C2 as mentioned...
- 10
- 439
- 0
Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: involvement of ATP release in astrocytes pdf
... Astrocytic Ca2+ oscillations and neuronal activities S Koizumi neuronal responses [24,26], and nearly correlated with the strength of the sensory stimulation Thus, neuronal activities also affect astrocytic ... apyrase [5,7] Third, visualization of the release of ATP demonstrates that the velocity of ATP release correlates well with that of the Ca2+ wave in astrocytes [5] All of these findings suggest that ... ‘gliotransmissions’, and ATP serves a central role as a gliotransmitter Now we understand that astrocytes receive and respond to neurotransmitters, and release gliotransmitter ATP to form synchronized and spreading...
- 7
- 549
- 0
Tài liệu Possible influences of air pollution, dust- and sandstorms on the Indian monsoon doc
... monsoon rains and droughts, as well as major industrial pollution and desert sand- and dust storms In the remainder of this article, we shall focus on aerosols in the IndoGangetic Plain and the Arabian ... easterly wind shear and a deepening of the Bay of Bengal depression Both are signals of a stronger South Asian monsoon (Webster and Yang, 1992; Goswami et al., 1999; Wang and Fan, 1999; and Lau et al., ... the foothills of the Himalayas, with the most pronounced increase over the Bay of Bengal and the western coastal region of India in June and July North-western India, Pakistan and the northern...
- 9
- 576
- 0
Tài liệu Báo cáo khoa học: Hypoxic resistance to articular chondrocyte apoptosis – a possible mechanism of maintaining homeostasis of normal articular cartilage pdf
... inhibition and TRAIL in osteoarthritic disease and chondrocyte apoptosis, and the hypoxic inhibition of cartilage and chondrocyte degeneration Results Macroscopic and radiographic examination of the ... chondrocyte apoptosis and may suppress the progression of arthritis Materials and methods Induction of OA Beagle dogs (n = 20) with a mean ± SD age of 1.4 ± 0.4 years and a mean ± SD weight of 10.2 ± 1.4 ... surfaces of natural killer (NK) cells, as well as on CD4+ and CD8+ T cells It promotes apoptosis, which may aid in the resolution of infection and the attenuation of the development of streptoxotocin-induced...
- 11
- 461
- 0
Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... equation of Fluo-3 for Ca2+ [27] Ultra-pure calcium-free water was used for preparation of all buffers and (ant)agonists Measurement of cytosolic cAMP and InsP3 Intracellular levels of cAMP and InsP3 ... thrombin-induced Ca2+ response, and abolished the effects of ADP, AR-C and wortmannin A B C D Fig Contribution of SERCA and phospholipase C to P2Y12-dependent prolongation of Ca2+ responses (A, B) Fura-2-loaded ... importance of the PI3-Kb isoform PI3-Kb and not PI3-Kc mediates P2Y12-dependent procoagulant activity of mouse and human platelets Prolonged elevation in [Ca2+]i can signal for surface exposure of phosphatidylserine,...
- 15
- 565
- 0
Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
... (2 lg) of QGP-1 cells using random hexamers and Superscript III (Invitrogen Corp.) One and two of 20 lL of the resulting single strand cDNA reaction were used as templates for RT-PCR and quantitative ... RNA of QGP-1 cells and pancreas (Fig 2A) Determination of the nucleotide sequences of the RT-PCR products revealed A Confirmation of utilization of exon 1a as a transcription initiation exon and ... fragments C and D, and 3¢ portion of 25 bp in intron in fragments F and I The number at the right of each RT-PCR product represents the length of the product The scheme also shows the location of the...
- 12
- 609
- 0
Tài liệu Báo cáo khoa học: Unfolding of human proinsulin Intermediates and possible role of its C-peptide in folding/unfolding pptx
... Unfolding of HPI in the presence of denaturant and thiol catalyst Controls for the disulfide scrambling method include the lack of thiol catalyst and lack of denaturant in the buffer As both controls ... Isolation and purification of the scrambled disulfide isomers of HPI In the presence of denaturant and thiol catalyst as indicated above, HPI was converted into the mixture of native and scrambled ... (preproinsulin) and folded as proinsulin, in which a connecting peptide of 35 residues links the C terminus of the B chain and N terminus of the A chain After digestion by a specific set of protein...
- 11
- 527
- 0
Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
... the activation of members of the MAP kinase family The latter relay, amplify and integrate signals from diverse stimuli, thereby controlling the genomic and physiological response of the cells ... via increased transcription and/ or by stabilization of the CTGF transcripts and the role of RhoA GTPase and p38 signaling in such a regulation, nuclear run-on assays and message stability analyses ... transcription rate of the CTGF/CCN2 gene was determined upon stimulation of the cells with S1P, anisomycin or FBS in the absence and in the presence of RhoA GTPase and p38 inhibitors (Y-27632 and SB-203580,...
- 15
- 576
- 0
Tài liệu Báo cáo khoa học: Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation doc
... of GAPDH and CP12 were used In lanes 1, and 4, lg of GAPDH ($ 0.08 nmol) and 0.08 lg of CP12 ($ 0.08 nmol) were mixed and lg of the mixture was analyzed In lane 2, 38 lg of the K128A mutant and ... was twofold higher, whatever the cofactor The kinetic parameters of the puried R197 mutant enzymes and those of the recombinant wild-type enzyme are shown in Tables and Circular dichroism and uorescence ... the superimposition of the Ca atoms of the wild-type GAPDH with those of K128A and K128E also Table Kinetic parameters of mutant GAPDH R197A and R197E, compared to those of the wild-type recombinant...
- 8
- 494
- 0
Tài liệu Báo cáo khoa học: A possible role of mitochondria in the apoptotic-like programmed nuclear death of Tetrahymena thermophila Takashi Kobayashi and Hiroshi Endoh docx
... (lanes 3, 4, and 5, respectively), 1, 5, and 10 mM EDTA (lanes 6, 7, and 8, respectively), and 0.1, 1, and mM ZnCl2 (lanes 9, 10, and 11, respectively) were assayed at 30 °C for 120 A standard reaction ... products (Fig 2A) and the parental macronucleus (Fig 2C and D) began to degenerate, they were stained in yellow (merged colour of green and orange), resulting from the fusion of the nuclei and lysosomes, ... lysosomes, and becomes acidic in the final step of PND [22,23] DePsipher staining of the macronucleus appeared initially during the stage of autophagosome formation, and persisted until resorption of...
- 10
- 642
- 0
Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc
... the 5¢-flanking region and determination of transcription start sites of mouse integrin a3 subunit gene The nucleotide sequence of the 0.5 kb SalI/SacI fragment and a part of exon is shown in Fig ... stretch of the sequence between the SalI and SacI sites upstream of exon 1, and that putative suppressor elements are present between the PstI (approximately 2.5 kb upstream of the SacI site) and ... CCAAT box at 324 bp upstream of the major transcription start site The presence of a CCAAT box and the absence of a TATA box seem to be one of the characteristics of the mouse integrin a3 gene...
- 9
- 562
- 0
Tài liệu Báo cáo Y học: Tissue factor pathway inhibitor A possible mechanism of action doc
... predicts too weak inhibition of the factor Xa activation and cannot describe the experiments of the study To test the adequacy of our model and the correctness of the values of the unknown constants ... verification Suppose that nM of Xa, nM of TFPI, or nM of the VIIa–TF complex are mixed together and activity of factor Xa is monitored In the absence of VIIa–TF, slow inhibition of Xa by TFPI will be ... equal to the concentration of the limiting component of the complex, TF The criteria for choosing of the values of the kinetic constants The values of the kinetic constants of those reactions whose...
- 16
- 415
- 0
Báo cáo khoa học: Involvement of lysine 1047 in type I collagen-mediated activation of polymorphonuclear neutrophils doc
... the shift of spots towards a lower pI was directly related to the carbamylation rate of peptides, each new spot corresponded to the modification of a new lysine residue Separation of control collagen-derived ... Incubation of PMNs with 100 lgÆmL)1 of type I collagen (black bar) was used as a positive control of PMN activation, whereas incubation of PMNs without effector was used as a negative control (white ... carbamylation rate of collagen CNBr peptides obtained from the incubation of collagen with cyanate and to establish a correlation between the number of spots detected and the number of modified lysine...
- 10
- 519
- 0
Báo cáo khoa học: 7-Ketocholesterol-induced apoptosis Involvement of several pro-apoptotic but also anti-apoptotic calcium-dependent transduction pathways ppt
... length and intensity of activation of ERK ⁄ is not sufficient to induce a detectable change in Bim concentration The short length of ERK activation and the dephosphorylation of Akt ⁄ PKB could be related ... h of treatment with 7-ketocholesterol alone (a 15–20% decrease of phosphorylated BAD), whereas cotreatment of THP-1 with the oxysterol and U0126 induced the same rate of dephosphorylation of ... treatment of THP-1 cells induced an increase of cytoplasmic free calcium [8], a time course of intracellular calcium fluctuations was performed between addition of 7-ketocholesterol and appearance of...
- 12
- 429
- 0
Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx
... induction of ArgII mRNA, protein and enzymatic activity in the context of virus infection in an IRF-3dependent and IFN-independent manner Moreover, expression of a constitutively active form of IRF-3 ... Activation of p38 mitogen-activated protein kinase and c-Jun NH(2)-terminal kinase by doublestranded RNA and encephalomyocarditis virus: involvement of RNase L, protein kinase R, and alternative ... single-strand RNA paramyxovirus known to be a strong activator of IRF3 phosphorylation [23] ArgII protein expression and arginase activity were detected at 24 h and increased 5–10-fold between 48 and...
- 12
- 498
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25