construction protein expression purification and ligation
Báo cáo khoa học: "Expression, purification and characterization of the Lily symptomless virus coat protein from Lanzhou Isolate" doc
... surveys Methods Strains, plasmids, and enzymes Expression and purification of LSV coat protein Figure Expression and purification of LSV coat protein A, Expression and purification of LSV CP 15% SDS-PAGE ... (BBI) and named pLCP312, analyzing and quantification have been carried out in agarose gel electrophoresis and in comparison with the standard molecular weight marker Construction of CP expression ... http://www.virologyj.com/content/7/1/34 Results and discussion RT-PCR and construction of expression vector The expected 900 bp cDNA encoding LSV CP was amplified by RT-PCR from isolated RNA The constructed plasmid for expression...
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Báo cáo Y học: RING finger, B-box, and coiled-coil (RBCC) protein expression in branchial epithelial cells of Japanese eel, Anguilla japonica pot
... NaCl/Pi and solubilized A glutathione S-transferase (GSt) fusion of eRBCC was expressed in E coli and assessed for its ubiquitination Cell culture and plasmid transfection Ó FEBS 2002 RBCC protein expression ... each protein relative to eRBCC are shown under the name of the protein The identity and similarity of the B30.2 domains are also shown Proteins compared with eRBCC are PwA33 [23], Xnf7 [5], and ... imaging plate for h and the result was analyzed with a BAS-2000 image analyzer (Fuji Film) Differentially expressed bands of interest were extracted from the gel and reamplified and then cloned into...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... contrast to expression of membrane proteins, the level of expression of cytosolic eGFP in the on-state was higher with pEBTet It follows that pEBTetD provides low background and moderate expression ... strategy is based on comparative analysis of lysates of cells both with and without transporter expression The expression of all other proteins in the two cell populations should match as closely as ... bases (244T > C and FEBS Journal 274 (2007) 783–790 ª 2007 The Authors Journal compilation ª 2007 FEBS 787 Fast set-up of inducible protein expression M Bach et al Fig Analysis of eGFP expression...
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Báo cáo " RECOMBINANT PROTEIN PRODUCTION: EXPRESSION SYSTEMS AND ANIMAL CELL TECHNOLOGY " potx
... Cell Protein Expression Applications High Throughput Protein Expression and Purification Butler, M (2004a) Growth and maintenance of cells in culture In Butler, M (Ed.) Animal cell culture and ... A., Lullau, E & Abbott, W M (2005) Optimisation and evaluation of a highthroughput mammalian protein expression system Protein Expression and Purification, 42, 111121 165 Kỷ yếu hội nghị Khoa ... suspension culture rapidly yield moderate quantities of recombinant proteins in protein- free culture medium Protein Expression and Purification, 36, 177-185 Gnoth, S., Jenzsch, M., Simutis, R & Lübbert,...
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Báo cáo khoa học: Transcription factor specificity protein 1 (SP1) and activating protein 2a (AP-2a) regulate expression of human KCTD10 gene by binding to proximal region of promoter pot
... basal promoter activity of KCTD10; and SP1 and AP-2a can regulate the promoter activity and endogenous KCTD10 expression oppositely through binding this region SP1 and AP-2a regulate KCTD10 Results ... if the expression changes of AP-2a or Sp1 alter the KCTD10 level, we checked endogenous KCTD10 expression in HeLa cells at the mRNA and protein levels using quantitative real-time RT-PCR and western ... Table 1, and subcloned into pCMV–HA at EcoRI ⁄ XhoI sites Plasmids pCMV–Sp1 and pCMV–AP-2a were confirmed by sequencing, and used to overexpress Sp1 and AP-2a in mammalian cells All expressed proteins...
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Báo cáo hóa học: "DNA polymeraseh protein expression predicts treatment response and survival of metastatic gastric adenocarcinoma patients treated with oxaliplatin-based chemotherapy" pot
... advanced gastric cancer patients [14-16] Oxaliplatin and cisplatin share the similar mechanism, and cause mono-adducts and intra-strand or inter-strand cross-links in the double DNA helix that severely ... relationship between polh protein expression and oxaliplatin sensitivity of gastric cancer cell lines and the significance of that in predicting treatment response and survival of metastatic ... gastric cancer cell (× 400) Table DNA polymerase h protein expression and clinical treatment response Table Correlation between POL h expression and clinicopathologic characteristics of gastric...
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báo cáo hóa học:" Low RBM3 protein expression correlates with tumour progression and poor prognosis in malignant melanoma: An analysis of 215 cases from the Malmö Diet and Cancer Study" potx
... recognition motif (RRM) and is able to bind to both DNA and RNA, whereby a glycine rich region adjacent to the RNA binding motif is thought to enhance the protein- RNA or protein- DNA interaction ... RFS and OS in both uni- and multivariate analysis Some subjects had no information on one or several markers and missing values were coded as a separate category for categorical variables and ... ~24.1%) [2] Immunohistochemical expression of RBM3 in primary tumours and metastases Of the 226 cases in the TMA cohort it was possible to evaluate the expression of RBM3 protein in 215 cases (95,1%)...
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ecombinant protein production expression systems and animal cell technology
... Cell Protein Expression Applications High Throughput Protein Expression and Purification Butler, M (2004a) Growth and maintenance of cells in culture In Butler, M (Ed.) Animal cell culture and ... A., Lullau, E & Abbott, W M (2005) Optimisation and evaluation of a highthroughput mammalian protein expression system Protein Expression and Purification, 42, 111121 Farrell, P & Iatrou, K ... suspension culture rapidly yield moderate quantities of recombinant proteins in protein- free culture medium Protein Expression and Purification, 36, 177-185 Gnoth, S., Jenzsch, M., Simutis, R & Lübbert,...
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Báo cáo y học: "Expression of cytokine mRNA and protein in joints and lymphoid organs during the course of rat antigen-induced arthritis" potx
... assessment and analysis of the protein data and participated in writing the manuscript AS, EB, and CBSW assessed and analyzed the mRNA data EPK critically read and edited the manuscript FE and RB ... decrease on day and a drop to below prearthritis values on days and day (Fig 3h) Cytokine mRNA and protein expression in the inguinal lymph node IL-1β Neither IL-1β mRNA (Fig 4a) nor IL-1β protein (Table ... cytokine and time point, the levels of mRNA and protein expression were compared with baseline levels (day 0) and with the respective preceding time point Correlations between cytokine mRNA levels and...
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Báo cáo y học: "Tumor necrosis factor α-induced adipose-related protein expression in experimental arthritis and in rheumatoid arthritis" pptx
... serum and (b) arthritic joints and (c) TIARP mRNA and protein expression in spleens (left and middle panels) and arthritic joints (right panel) by real-time polymerase chain reaction (PCR) and ... and protein was noted at days 14 and 28, and the expression correlated with joint swelling (Figure 2c, right Figure Serial changes in expression levels of tumor necrosis factor-alpha (TNFα) and ... patients with RA and OA, we investigated the expression of STEAP4 mRNA in synovium of patients with RA and OA Relative expression of STEAP4 was almost comparable between RA and OA, although expression...
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Báo cáo y học: " Effect of HIV-1-related protein expression on cardiac and skeletal muscles from transgenic rats" docx
... tissues to detect levels of the thiol pairs GSH and GSSG, and Cys and Cyss HIV-1-related protein expression had no effect on GSH or GSSG levels (A and B), but did increase the overall oxidative ... plantaris muscles to detect levels of the thiol pairs GSH and GSSG, and Cys and Cyss HIV-1-related protein expression increased the levels of GSSG (B) and Cyss (E) compared to controls Surprisingly, GSH ... IIa, IIx, and IIb) and while diffuse atrophy has been reported here and in the literature [18], the subtle morphological and genetic differences between the mouse and rat transgenic models and the...
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báo cáo khoa học: "EGFR and COX-2 protein expression in non-small cell lung cancer and the correlation with clinical features" pptx
... On the other hand, COX-2 could induce the activation and expression of EGFR The lack of correlation of EGFR and COX-2 expression in our study implied that the expression of these proteins might ... http://www.jeccr.com/content/30/1/27 Results EGFR protein expression Page of Table Comparing EGFR protein expression in neoplastic and normal tissue The positive rate of EGFR protein in NSCLC tumor cells were ... immunohistochemically for EGFR and COX-2 expression and their prognostic values were explored after deparaffinization and rehydration, the samples were treated with sodium citrate buffer and microwave for...
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báo cáo khoa học: " Correction: EGFR and COX-2 protein expression in non-small cell lung cancer and the correlation with clinical features" pps
... (corrected table 3) EGFR expression and clinical characteristics Page of Table (corrected table six) COX-2 expression and correlation with clinical features EGFR Positive expression rate P value ... Yongmei, Chen Huijiao, Liao Dianying, Shen Yali, Xu Feng, Wang Jin: EGFR and COX-2 protein expression in non-small cell lung cancer and the correlation with clinical features Journal of Experimental ... doi:10.1186/1756-9966-30-32 Cite this article as: Li et al.: Correction: EGFR and COX-2 protein expression in non-small cell lung cancer and the correlation with clinical features Journal of Experimental...
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báo cáo khoa học: "ShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF-7 in vitro" pptx
... constructed and transfected into the breast cancer cell lines MDA-MB-231 and MCF-7 Its effect on protein expression of estrogen recepter alpha(ER a ), matrix metalloproteinase 9(MMP-9), cyclinD1, and ... MDA-MB231, and protein levels of MMP-9 and CyclinD1 were down-regulation (P < 0.05) However, in ER alpha-positive breast cancer cells MCF-7, protein expression levels of ER alpha, MMP-9 and CyclinD1 ... metalloproteinases mRNA and protein expressions in lung cancer cells (A549) MTA1, a aid activation factor of histone deacetylase might down-regulate MMP-9 expression level by direct manner and by...
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Báo cáo y học: "Altered protein expression in serum from endometrial hyperplasia and carcinoma patients" pdf
... (SEH, CEH, and AEH) and ECa We found several proteins with altered expression levels during disease progression that could represent serum biomarker candidates in EH and ECa Results and discussion ... cancerous tissues Three additional proteins, apolipoprotein C-II precursor, apolipoprotein E precursor, and apolipoprotein AIV precursor, showed consistently altered expression with high confidence ... groups (Figure 2) Upregulation of apolipoprotein C-II precursor and apolipoprotein E precursor in SEH and downregulation of apolipoprotein A-IV precursor in CEH and AEH were of significance according...
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Báo cáo y học: " Human cellular microRNA hsa-miR-29a interferes with viral nef protein expression and HIV-1 replication" ppsx
... hsa-mir-29a and b inhibited Nef expression and HIV-1 replication hsa-mir-29a and b inhibited Nef expression and HIV-1 replication (A) Nef expression was inhibited by hsa-miR-29a and 29b HEK293T ... hsa-mir-29a and 29b pEGFP-miRNA construct expressing hsa-mir-29a and 29b (A) Diagrammatic representation of pEGFP-N3 vector containing pre-miR29a and 29b (B) Expression of hsa-mir-29a and 29b confirmed ... hsa-miR-29a and b, along with pCDNA-HA-Nef into HEK293T cells and analyzed Nef expression by immunoblotting As shown in Fig 4A, both the hsa-miR-29a and 29b expressing clones reduced the expression...
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Báo cáo y học: " Binary gene induction and protein expression in individual cells" pptx
... 10 Protein Expression Level (AU) Figure mRNA and protein half-lives and induction time on the appearance of protein expression histograms Effect of5 Effect of mRNA and protein half-lives and ... Induction Time (h) Figure mRNA and protein Effect ofexpression protein half-lives on the dynamics of Effect of mRNA and protein half-lives on the dynamics of protein expression (A) Binary switching ... Protein Expression Level (AU) Figure protein detection sensitivity on the appearance of protein expression histograms Effect of7 Effect of protein detection sensitivity on the appearance of protein...
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Báo cáo y học: "Quantitative protein expression profiling reveals extensive post-transcriptional regulation and post-translational modifications in schizont-stage malaria parasites" doc
... parasite and two human proteins, with 15 of these proteins having been encountered in more than one protein spot (Figure and Table 2) The limited scope of identified proteins notwithstanding, ... trophozoitespecific proteins Protein expression profiles and mRNA levels Figure For the parasite proteins identified by mass spectrometry, we then compared the DIGE protein expression profiles ... Expression profiles comparing relative mRNA and protein abundance Expression profiles comparing relative mRNA and protein abundance The panels summarize mRNA and protein abundance data for individual...
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Identifying protein co factors of oct4, an essential stemness transcription factor, by affinity purification and mass spectrometry
... Sp1 Affinity Purification/ Tandem MS/MS - - Affinity Purification/ Tandem MS/MS (Wang et al., 2006) - - Affinity Purification/ Tandem MS/MS (Wang et al., 2006) - - Affinity Purification/ Tandem MS/MS ... this approach The expression of proteins in E.coli and use of large expression tags like GST and maltose binding protein (MBP) may not be suitable for all eukaryotic proteins, and causes misfolding ... fusion with a bait protein of interest The first Tandem Affinity Purification (TAP) tag was placed at the N and C-terminal ends of a yeast protein SmX4p, and contained epitope tags Protein A (from...
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Purification and cloning of PCR products
... vector, reagents for ligation and competent cells and are also available in TOPO-Cloning™ format to enhance ligation efficiency and speed As described above, there are rapid ligation components ... times for cohesive and blunt-end ligations Examples include rapid DNA ligation kits from Roche, Fermentas and Epicentre There is a potentially useful protocol for temperature-cycle ligation (9) in ... pENTRY Add expression vector pDEST to pENTRY clone LR clonase reaction to create expression clone Target sequence att B1 att B2 Expression clone Purification and cloning of PCR products 131 and attB2...
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