conditions with altered hpa axis activity
Báo cáo khoa học: Apolipoprotein E-derived antimicrobial peptide analogues with altered membrane affinity and increased potency and breadth of activity pdf
... activity against S aureus, and Cys substitutions maintained anti-HSV1 activity Other substitutions for Leu reduce or abolished antiviral or antibacterial activity In general, anti-infective activity ... berghei to cells, with this most likely involving increased membrane perturbation associated with their aromatic groups Results We examined the influence of peptide length on activity using a series ... residues were critical for its activity Replacing either all the Lys or all the Arg residues with a hydrophobic residue (Trp), or replacement of both Lys and Arg with His or the acidic residues...
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Tài liệu Báo cáo khóa học: UDPgalactose 4-epimerase from Saccharomyces cerevisiae A bifunctional enzyme with aldose 1-epimerase activity docx
... cells with galactose, there was 3.5-fold increase in epimerase activity (Fig 4B) while the constitutive mutarotase activity remained the same More importantly, with the increase in epimerase activity, ... epimerase activity also showed mutarotase activity If S cerevisiae epimerase were a truly bifunctional enzyme, then induction with galactose should induce both epimerase and mutarotase activity ... (ORFs) YHR210c and YNR071c, with putative aldose 1-epimerase activity These ORFs encode for proteins, each with a predicted molecular mass of 38-kDa, and exhibit 99% identity with the human aldose...
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Tài liệu Báo cáo khoa học: Tet repressor mutants with altered effector binding and allostery docx
... 4-ddma-atc and rescreened for induction with 0.4 lm atc and without inducer on MacConkey agar plates We screened 17 600 colonies with mutations in helix a1, 29 840 with mutations in helix a4 and 3900 ... repression TetRi2-E15A L17V shows repression to 12.5% with 4-ddma-atc and to 58% with atc, thus showing only fivefold improved repression with 4-ddma-atc over atc A similar result is found for ... L60M yield a TetRi2 mutant with reduced inducibility TetRi2-I59V H63Q exhibits a reverse phenotype but only little specificity with fourfold increased corepression with 4-ddma-atc over atc The...
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Tài liệu Báo cáo khoa học: A novel nuclear DNA helicase with high specific activity from Pisum sativum catalytically translocates in the 3¢fi5¢ direction docx
... contamination with nuclease activity Fraction III was applied to a phosphocellulose column equilibrated with buffer A Following washing with buffer A, the bound proteins were eluted with a linear ... still contained nuclease activity as shown in Fig 1B as DP The active fractions were pooled and diluted with buffer A without KCl (fraction III, 124 mL) Up to this step the activity was not quantified ... 0.1 M with buffer B, fraction V was loaded onto a ssDNA-cellulose column equilibrated with buffer B After washing the column excessively with buffer B the bound proteins were eluted in steps with...
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HPA axis responses to laboratory psychosocial stress in healthy elderly adults, younger adults, and children: impact of age and gender ppt
... gender-specific patterns of HPA axis responsivity points at the necessity to clarify what the applied tests exactly measure and which levels of the HPA axis are activated While most HPA axis stimulation ... in hypothalamic–pituitary–adrenal (HPA) axis regulation, it still remains an open question whether stress-related HPA axis functioning alters significantly with age While in humans there are only ... chosen test, for example testing HPA axis reactivity or its maximum capacity Reported gender differences could possibly be attributed to differences in the applied HPA axis stimulation procedures...
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Báo cáo khoa học: Human retinol dehydrogenase 13 (RDH13) is a mitochondrial short-chain dehydrogenase⁄reductase with a retinaldehyde reductase activity pdf
... substrates, with NADP+ ⁄ NADPH as the preferred cofactors However, the substrate and cofactor specificity of RDH13 remains unknown, as it failed to exhibit any enzymatic activity under the conditions ... catalytic activity of RDH13 by expressing the protein in Sf9 cells as a fusion with the C-terminal His6 tag in order to purify RDH13 to homogeneity and characterize its properties under well-defined conditions ... reductase activity was associated with RDH13 expression, we purified RDH13–His6 using Ni2+ affinity chromatography This single-step purification procedure produced an almost homogeneous protein (Fig 4) Activity...
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Báo cáo khoa học: NMR solution structure of Cn12, a novel peptide from the Mexican scorpion Centruroides noxius with a typical b-toxin sequence but with a-like physiological activity doc
... further separated by HPLC using previously published conditions [34] Lethality tests were conducted with mice, crickets and crayfish, using the same conditions as reported [34] Determination of amino-acid ... Cn12 with other Na-ScTxs A databank search with the sequence of Cn12 as the query retrieved only scorpion venom-derived peptides classified as Na-ScTxs The highest identities were found with scorpion ... allow proper discussion in the terms of structure activity relationship, with regard to recognition and affinity for Na+ channels, Cn12 is aligned with other Na-ScTxs in Fig For this comparison,...
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RUBBER PRODUCTION IN LIBERIA: An Exploratory Assessment of Living and Working Conditions, with Special Attention to Forced Labor docx
... of Living and Working Conditions, with Special Attention to Forced Labor Introduction With support from the U.S Department of Labor, Verité carried out research on labor conditions in the supply ... negotiated with Firestone to provide children living on the plantation with better schools.54 Rubber Production in Liberia: An Exploratory Assessment of Living and Working Conditions, with Special ... political groups within worker communities Initial communications and interviews with workers Rubber Production in Liberia: An Exploratory Assessment of Living and Working Conditions, with Special...
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Báo cáo hóa học: " Human cord blood progenitors with high aldehyde dehydrogenase activity improve vascular density in a model of acute myocardial infarction" ppt
... b2m null mice with AMI were transplanted with ALDHloLin- or ALDHhiLin- sorted human UCB cells or PBS Frozen sections were stained with a mouse specific CD31 antibody and visualized with DAB+ chromagen ... with a small cytoplasm relative to the nucleus We found no cells with cardiomyocyte morphology in the 150 individual sections analyzed Staining for human hematopoietic and endothelial cells with ... heart of NOD/SCID b2m null mice with AMI four weeks after transplantation of ALDHhi Linsorted human UCB cells NOD/SCID b2m null mice with AMI were transplanted with ALDHhiLin- sorted human UCB...
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Báo cáo y học: " Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats" ppt
... placed on the cross-talk between inflammation and the HPA axis Studies have shown that inflammatory cytokines (IL-1, IL-6 etc) activate the HPA axis with the increase of secretion of cortisol, which ... studies are needed to clarify how the HPA axis response to the change of asthma-related cytokines and other inflammatory mediators, and how the HPA axis communicates with neural and endocrine networks ... [19] The activation of the HPA axis is also observed in allergic rhinitis during nasal provocationand and in cutaneous inflammatory disease [20,21] Thus, the activated HPA axis inhibits the inflammation...
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Báo cáo y học: "Decreased catalytic function with altered sumoylation of DNA topoisomerase I in the nuclei of scleroderma fibroblasts" ppsx
... SUMO1 with and without SUMO1 siRNA transfection in fibroblasts Three SSc fibroblast strains (two with anti-topo I and one with anti-RNA polymerase III positive serum) were transfected with SUMO1 ... proteins were sumoylated with either wild type SUMO1 or mutant SUMO1 or negative control (without sumoylation) and then were examined with Western blot for sumoylated topo I and with topo I catalytic ... recombinant human topo I with and without sumoylation reaction Recombinant human topo I proteins were sumoylated with either mutant sumo1 or wild type sumo1 or negative control (without sumoylation),...
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Báo cáo y học: " Low NO bioavailability in CCl4 cirrhotic rat livers might result from low NO synthesis combined with decreased superoxide dismutase activity allowing superoxide-mediated NO breakdown: A comparison of two portal hypertensive rat models wit
... able to react with NO to form reactive nitrogen species, which could not have vasodilatory effects Superoxide dismutase competes with NO to react with superoxide Superoxide dismutase activity leads ... (Table 2) Hepatic superoxide dismutase (SOD) activity SOD activity in liver homogenates in buffer I was diluted 400 times with buffer I and measured with a RANSOD kit (Randox Laboratories, Crumlin, ... protein amounts, is the understanding of eNOS enzymatic activity [18] The eNOS activity is inhibited by protein-protein interaction of caveolin-1 with eNOS in hepatic [7,12] and extrahepatic vessels...
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Báo cáo y học: "Liposarcoma cells with aldefluor and CD133 activity have a cancer stem cell potential" pot
... cells could be associated with CSC activity We preferred to use a xenograft model, because the passing of the xenograft from mouse to mouse ensures that the growth conditions are physiological ... been associated with stem-ness and tumor initiating activity [36,37] Interestingly, when the cells had been maintained in SC-medium, a larger percentage of the cells displayed ALDH activity (average ... SW872 xenograft-derived cells (98, 8%) were sorted on the basis of (B) Aldefluor (X -axis) and CD133 (Y -axis) activity In this representative experiment the subpopulations in the culture were as...
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Báo cáo y học: "An improved method for detecting and delineating genomic regions with altered gene expression in cancer" ppsx
... Materials and methods) Left: Example signal generated with 40-probe step with SNR 2.0, and no non-influenced genes (π = 0.0) Right: Corresponding signal with a higher proportion of non-influenced genes ... well for a broad range of conditions, including low SNRs which is the most relevant case here In agreement with [24], we found CGHseg to perform better than, or on par with, DNAcopy (data not shown) ... profile across within each class, as determined from the Mullighan et al data set [29] (Affymetrix 250 k SNP arrays) Key observation: The method successfully identified several regions with altered...
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Báo cáo y học: "Getting out and about in older adults: the nature of daily trips and their association with objectively assessed physical activity" doc
... daily trips, with participant characteristics and with accelerometry-assessed daily physical activity We have combined objective data from a diverse sample of older adults on physical activity, ... from the home are associated with objectively measured physical activity, both as volume of MVPA, and steps per day Shopping is an important activity, and combined with trips for personal business ... on structured programmes of physical activity [7] much less is known about daily patterns of movement and their association with overall levels of physical activity [8] Recent evidence from Japan...
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Báo cáo y học: "Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes" pptx
... correlations with glucose, 626 positive and 408 negative correlations with sucrose, and 579 positive and 371 negative correlations with glucose-6phosphate in the combined data set More genes correlate with ... These two enzymes and NADP-glyceraldehyde-3P dehydrogenase activity also respond differently in pgm and in a prolonged night, with activity being lower during the diurnal cycle in pgm, especially ... also changes in these metabolites within the diurnal cycles, and that these are not related to momentary changes in the enzyme activity (as measured in optimized conditions in vitro) Thus, changes...
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Báo cáo sinh học: "properties related Drosophila melanogaster strains with altered fitness also show changes in their hobo element " doc
... gels experiments RESULTS Male mating activity As some of the strains under investigation are kept without any further selection with respect to their male mating activity, we first performed a series ... III) Another strain with decreased activity, LA6-, shared only (6.5%) and (8.9%) sites with LA and LApas, respectively Only sites at 17E and 96E were common to all The high activity strains HA, ... selection for low male mating activity, harboured active hobo elements in its genome We extended this analysis to encompass LA-derivative strains with altered male mating activity, and in this report...
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Second order optimality conditions with the envelope like effect in nonsmooth multiobjective mathematical programming i l stability and set valued directional derivatives
... optimality conditions with envelope-like effect for nonsmooth vector optimization in infinite dimensions, Nonlinear Anal 77 (2013) 130–148 P.Q Khanh, N.D Tuan, Second-order optimality conditions with ... establish in Section necessary optimality conditions, with the envelope-like effect, for local weak solutions of (P) Section contains sufficient optimality conditions for local firm solutions In ... spaces Second-order optimality conditions with the envelope-like effect are obtained also for local weak and firm solutions, but in terms of other generalized derivatives, with a higher level of nonsmoothness...
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Báo cáo y học: "Relationship between Anti-CCP Antibodies and Oxidant and Anti-Oxidant Activity in Patients with Rheumatoid Arthrit"
... blood and serum antioxidative activity (CAT, GSHpx) and the mean blood and serum MDA and MPO values (oxidative activity) , between the patients with anti-CCP(+) and those with anti-CCP(-) (Table 2) ... positivity seems to be associated with increased synovial fluid oxidant activity (increased MDA and MPO levels) in patients with RA And this increased oxidative activity in synovial fluid may be ... synovial fluid, there was increased synovial oxidant activity (MPO and MDA levels) (p
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Báo cáo y học: "Serum cholesterol concentration associated with aspirin esterase activity in older people: preliminary data"
... serum total cholesterol concentrations are clearly associated with those of aspirin esterase activity during a period of physical activity modification in an older population This finding suggests ... whole circulating aspirin esterase activity as compared to that of BChE (8), and our study population did not include subjects with malnutrition nor there were any with nutritional modifications ... association of changes in circulating total cholesterol with those of serum aspirin esterase activity in an older population that participated in a physical activity modification program Although aspirin...
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