... the marking should give you a short accurate summary ofthe text Sample of Underlining and highlighting: The government says the average American man eats ten grams of salt a day The American ... ina passage Reading to learn - Recognizing the organization and purpose ofa passage - Understanding relationships between ideas - Organizing information into a category chart or a summary in ... radar, an alternative way of navigating Since human eyes are not very good at determining speeds of approaching objects, radar can show a pilot how fast nearby planes are moving The basic principle...
... Bhagwat SV, Biswas G, Anandatheerthavarada HK, Addya S, Pandak W & Avadhani NG (1999) Dual targeting property ofthe N-terminal signal sequence of P450 1A1 Targeting of heterologous proteins to ... in endogenous signalling pathways and environmental carcinogenesis Nat Rev Cancer 6, 947–960 Addya S, Anandatheerthavarada HK, Biswas G, Bhagwat SV, Mullick J & Avadhani NG (1997) Targeting of ... were initiated by the addition of mm NADPH, and incubation was continued for 30 at 37 °C ina shaking water bath The reactions were terminated by adding mL of ice-cold methanol, and insoluble particles...
... When the assay contained valinomycin, the data reported in Fig 1B were obtained In this case, the ATP yield of both wild-type and mutant as a function of illumination time presented a lag phase ... appreciated by taking the derivative ofthe fitting functions ofthe mutant and wild-type data and plotting their ratio, as in Fig 1C This derivative represents the rate of ATP synthesis at each ... RESULTS The single point mutation aE210K was introduced into the atp2 operon of Rb capsulatus, containing the F0 genes, cloned in an E coli strain The mutated operon was then transferred into a broad-host-range...
... between A- and B-chains In WT thrombin, the A- chain assumes an overall boomerang-like shape interacting with the B-chain surface opposite to the active site [17] Stabilization within the A- chain and ... steady-state amidase activity and binding ofthe high-affinity inhibitor a- NAPAP showed pKa values ofthe catalytic His57 higher in DK9 mutant than in WT thrombin Application ofthe Brønsted theory ... intra- and interchain bonds After 180 denaturation with m urea and 0.2 mm b-mercaptoethanol, the A- chain was released andthethe native enzyme form disappeared for both WT and mutant thrombin...
... bond ˚ and Asp140 is 10.7 A Mutational effects The residues mutated in this study are shown in Fig Asp140, Asp142, Glu144 and Asp215 were mutated individually to asparagine and alanine, Tyr10 and ... inthe pH 4.2– 8.0 range, anda marked increase in Km at alkaline pH (Table 2, Fig 3) The D215N and D140N mutants displayed an acidic shift inthe pH-activity profiles, whereas the Y214F andthe ... [26,29] The pKa calculations indicate that Glu144 has a slightly elevated pKa inthe free enzyme that, at least in part, results from the vicinity of Asp215 (Table 3, rows and 4) The calculations indicate...
... or was obtained from Oriental Yeast Co (Lot 21003805) (Osaka, Japan) and proCPY was prepared as the same manner as CPY, with minor modifications Dgly CPY and Dgly proCPY, in which the asparagine ... Isolation and physicochemical properties of yeast proteinase Aand C Agric Biol Chem 31, 357–367 Aibara, S., Hayashi, R & Hata, T (1971) Physical and chemical properties of yeast proteinase C Agric ... respectively) In contrast, a DSC analysis ofthe mature form (CPY) revealed an apparently symmetrical single peak but the ratio of DHcal/ DHv was determined to be 1.74 (DHcal and DHv values were 765 and...
... critical roles inthe formation and accumulation of mtDNA deletions inthe inner ear The marked increase in mtDNA replication caused by TFAM overexpression inthe inner ear may also take part in ... that the capacity to repair oxidative DNA damage in various brain regions during aging was altered in an age-dependent manner, and increased mtDNA oxidative damage might contribute to the normal ... explain the increased mutation load inthe inner ear In our study, increases in TFAM expression and deletion mutation load were demonstrated inthe inner ear of d-Gal-treated rats The replication...
... contact area ofthe Nt amidinium end (NAE) was investigated by evaluating the interaction energies and hydrogen positions ofthe end fragment andthe bases of base pair T8 -A2 5 and base G9 (Table ... calculations ofthe interaction between (A) the amidinium end and bases A2 5, T8 and G9; and (B) the guanidinium end and bases A5 , T28 and A6 Intermolecular geometry constraint according to the crystal structure ... netropsin structures To date, 14 different structures containing Nt have been found inthe NDB of which nine contain Aand T bases inthe central part ofthe DNA (seven contain an AATT tract and...
... bootstrap analysis ofthe sequence data with the same software To evaluate the pathogenic activity ofthe strain, the aseptically cultured potato microtubers in vitro were used as described by Lawrence ... fi6 )a- D-Glcp-(1fi4)-b-D-ManpNAc3NAcA-(1fi was the major component ofthe cell wall preparation The absolute configuration of glucose (D-) isolated after hydrolysis ofthe total cell wall preparation was determined ... essential for their growth taxis and their attachment to potato tuber The presence of Kdn might be characteristic of plant pathogenic streptomycete strains causing scab diseases of potatoes and...
... that “There is clearly a need for more, and better quality, data and this is particularly acute inthe case of natural language data” and “learner corpora are a valuable addition to current SLA ... typically say, but also what the typical difficultiesofthe learners ofa certain language, or rather of certain groups of learners of this language, are 12 As seen above, there is a wide consensus ... as Language Learning and IRAL) would indicate that the advocates of EA have prevailed and that EA currently appears to be the “darling” ofthe 70’s However, EA was not without problems It was...
... substrate Therefore, the metal ion has a catalytic rather than just a substrate binding role Possible roles ofthecation include the activation of water for the hydration reaction and ⁄ or the stabilization ... Ontario, Canada) or New England Biolabs (Pickering, Ontario, Canada) All other chemicals were of analytical grade and were obtained from Sigma-Aldrich and Fisher Scientific (Nepean, Ontario, Canada) ... pathway [5] The binding of divalent cations by the decarboxylase precludes metal binding studies ofthe hydratase in that complex In this study, BphH, the hydratase inthe PCBs degradation pathway...
... principle of aesthetic education, directing education not against capitalism, but against the rei cationof reason (177–79) Marcuse incorporated psychoanalysis into educational and aesthetic theory ... reappraisal ofthe validity ofthe culture ofthe bourgeois era.” He speaks of art as a “second alienation,” which is “emancipatory rather than oppressive.” Here, the affirmative character of art ... liberatory power of art against the prosaic routine of daily life He argues that revolutions in art and culture—manifestations ofthe rebellious spirit ofthe aesthetic imagination—can fuel social-protest...
... and assistant health staff The values analysed refer to the basic annual salary for each year The changes in contractual salaries and related purchasing power were analysed by means of an income ... The authors gratefully acknowledge the assistance of Dra Ana Flavia Machado, Professor of Demography at the University of Minas Gerais, in analysis ofthe data and for her helpful comments and ... involved inthe conceptualization, initial drafts and final write-up ofthe paper All authors had access to all data inthe study and 18 19 Fritzen AS: Strategic management ofthe health workforce in...
... morphology and topology For the morphological characterisation of shoots and roots andthe topological characterisation of roots, 22 seedlings were taken randomly per type of container at the end ofthe ... irrespective of container type The slope ofthe linear regression of F to P was significantly smaller in ST than in LT and MT, indicating that lower flows were obtained in ST for a particular pressure value ... Continual air pruning induces a limitation to the main root growth by shortening its length to the depth ofthe container and preventing the development of replacement taproots The confining of lateral...
... Study area The study area was located inthe mixedwood section ofthe southern boreal forest of Saskatchewan, Canada The area falls between 53° 38’ and 54° 41’ N latitude, and 105° 00’ and 106° ... providing the basal area andthe recruitment ofthe second wave of seedlings or advanced regeneration associated with the opening ofthe canopy Logs created by the death of P tremuloides andthe ... resultant canopy gaps formed, appear to be critical for P glauca regeneration in mature and old stands Natural regeneration may be enhanced by taking advantage ofthe advanced regeneration during...
... values, inconsistent data, claim duplicates, and unexpected missing values The SCAP database is similar to other administrative and pharmacy claims database, as it provides detailed information about ... olanzapine treatment group had a clinically meaningful anda statistically significant lower rate of hospitalization and fewer hospitalized days during the year following initiation We found a mean ... http://www.general-hospital-psychiatry.com/content/3/1/11 The National Institute of Mental Health (NIMH) [14] has emphasized the need to take findings generated by clinical research and translate them into treatment for patients who are seen in day-to-day...
... While there are many differences inthe molecular and genetic details ofthe circadian machinery in mammals and Drosophila, the basic regulatory principles are maintained [20,21] Central to the ... forward 5'-GTGAACGCCGTGCACTGGTTCCGAAAGGGAC-3', mCry1 reverse 5'GTCATGATGGCGTCAATCCACGGGAAGCCTG-3'; mPer2 forward 5'-GATCAGCTGCCTGGACAGTGTCATCAGGTACC-3', and mPer2 reverse 5'-CTGAGCGTCGAGGTCCGACTAGGGAACTCAGCC-3' ... participated inthe design ofthe study, and drafted the manuscript BD generated the RNAi GSK3 mutant lines (A1 .4 and A1 .6), generated the c2.1 3/4 DKO mutant, and carried the clonal Western analysis...
... Sasaki H, Takada E, Sunagawa M, Masawa N: Fine needle aspiration of toxoplasmic lymphadenitis in an intramammary lymph node A case report Acta Cytol 2001, 45:259-262 Siriwardana et al Journal ... produces a wide range of clinical syndromesin humans, land and sea mammals, and various bird species Toxoplasmosis passes from animals to humans, mainly via infected cat feces T gondii infects a large ... biopsy The results ofa histological examination (Figures and 2) ofthe resected specimens of breast and axillary lesions were suggestive of an intramammary and axillary lymph node mass with marked...