... ComparisonoftheeffectsofpHLandLeoontheexpressionof apoptosis- relatedproteinsinthehippocampusfollowingTBI 79 ix CHAPTER FOUR DISCUSSION 81 CHAPTER FIVE CONCLUSIONS, ... ComparisonoftheeffectsofpHLandLeoonthe activities of SOD, CAT, GPx and GST inthe cortex followingTBI 78 3.3.2 Comparing the anti-apoptotic effectsofpHLandLeo 79 3.3.2.1 Comparison ... pathways involved inTBI 96 5.3.2.1 pHL or Leoontheexpressionof apoptotic pathway proteins at earlier time points ofTBI 96 5.3.2.2 The role of iInflammation inTBI ...
... Osteoarthritis, of these 30 ofthe 48 had radiographic Grade (bone on bone) arthritis, the remaining 18 had grade OA Ofthe 48 patients, four had undergone revision, four had died since implantation and ... positioning ofthe femoral component andthe Oxford score (0.38) Examples of good and poorly positioned prosthesis can be seen in Figures and Edmondson et al Journal of Orthopaedic Surgery and ... this study All contributors were fully involved with the preparation and analysis ofthe results of this study I would like to acknowledge the help of Matthew Hankins ofthe Brighton and Sussex University...
... crystallization solutions The ligand bound was interpreted as GMP because the N2 ofthe base makes a hydrogen bond to a main chain carbonyl Binding of a xanthine base from XMP inthe same position, would ... (Fig 1C) The 2¢OH ofthe ribose is hydrogen bonded to the main chain andthe side chain of Asp142 (Fig 1C) Both hydroxyl groups ofthe ribose are involved in coordinating a putative Ca2+ ion This ... second Ca2+ ion is coordinated by the side chain of Asp201, a phosphate ion and five water molecules The phosphate involved in this interaction is bound by main chain interactions with Lys76 and...
... monomer (Table 1) The sub-stoichiometric iron content of iron–sulfur proteins is generally due to the impaired incorporation ofthe cluster into the protein during overexpression in E coli and ... disintegration ofthe iron–sulfur cluster in each ofthe WhiB proteins (Fig 3C,D) Therefore, disassembly ofthe iron–sulfur cluster under oxidizing conditions and its stability under reducing conditions ... Therefore, we assume that in WhiB proteins, one disulfide bond is formed between the two cysteines ofthe CXXC motif (CXXXC inthe case of WhiB5) andthe other between the remaining two cysteines...
... inthe segment of residues 242–245, bringing the polypeptide chain back to the same subunit We analyzed the interactions of corresponding residues in SurEs to understand the reasons for domain ... form The shift inthe position ofthe loop away from the metal ion leads to the formation of two interprotomer active sites where the Oe of E37 from one subunit coordinates with the metal ion bound ... Y238 in Aa SurE The hydroxyl group ˚ of Y238 is hydrogen bonded to D234 OD1 (2.55 A) The other interactions of D234 in Aa SurE correspond to the interactions of D230 in St SurE A different set of...
... of new therapies and ⁄ or vaccines, or the improvement of existing ones, necessary to control this disease The identification and characterization oftheproteins involved inthe interaction of ... determination of some oftheproteins present onthe membrane for which no role in M tuberculosis invasion ofthe host cell has yet been determined [12,13] The infection of epithelial cells inthe ... (in this case), their binding dissociation constants, their critical binding residues and their role in cell invasion inhibition, with the specific purpose of employing them as probable components...
... forms of LPL reveal interesting features of LPL structure, including the conformational change inthe active center, the critical sites for heparin binding, andthe orientation of dimerization Investigation ... The cluster of basic residues inthe C-terminal domain may constitute heparin binding-site residues that coordinate with heparin-binding residues inthe N-terminal domain The cluster of hydrophobic ... subunit andthe substrate recognition site inthe C-terminal domain ofthe other face each other, thereby bringing together the substrate-binding and catalytic functions in proximity for effective enzyme...
... Cloning and DNA manipulation The reaction mixture for PCR amplification ofthe ppa gene from Synechocystis sp PCC 6803 (50 lL) contained 50 pmol of each ofthefollowing two pairs of oligonucleotide ... Gingrich JC, Gasparich GE, Sauer K & Bryant DA (1989) Nucleotide sequence andexpressionofthe two genes encoding D2 protein andthe single gene encoding the CP43 protein of photosystem II in ... (MgCl2) and mm Na4PPi (standard assay conditions) The reaction was started by the addition of enzyme, andthe PPi released after 10 was determined When the efficiencies of other divalent metal cations...
... Akt and Erk signalling pathways in mediating theeffectsofthe gonadotropins and IGF on follicle cell function andon follicular development The stimulation of inhibin-A, activin-A, follistatin, ... actions ofthe Akt pathway are more pronounced than the Erk pathway in granulosa cells and vice versa inthein theca cells None the less, administration of inhibitors of these pathways in vivo inhibited ... PG: Effectsof active immunization of sheep against an amino terminal peptide ofthe inhibin αC subunit on intrafollicular levels of activin A, inhibin A and follistatin Journal of Endocrinology...
... patients andthe final grading of their elbow and shoulder function are detailed in Table Table details the motor grade ofthe recovered muscles andthe final range of movement achieved Table shows the ... supraspinatus muscle which is only an abductor attracts all the axons and reduces the potential of reinnervation ofthe external rotators The other explanation is, the antagonistic muscle- for instance, ... Authors' contributions HR and SRS were the main operating surgeons and designed the study, PB and SRJ performed data collection and analysis ofthe results, HR and PB were involved in sequence...
... embedded in araldite Semithin resin transverse and longitudinal sections were stained using haematein and eosin, solochrome blue and paraphenylene diamine Ultrathin sections were double stained with ... in all of these patients to determine the cause of limb weakness and diaphragm dysfunction The medical committee ofthe hospital approved the study, and informed consent was obtained from the relatives ... survivors the absence of CMAPs on electrophysiological examination could suggest prolonged functional disability Because ofthe lack of sensitivity of clinical examination in such patients, combined...
... ventricular drains) on admission to the neurocritical care unit (thereby explaining that withdrawal of consent would probably have correlated with rapid clinical worsening andthe probability of early ... contributions ICK and HBH designed the study and wrote the manuscript ICK, JBK, LB, and CM obtained clinical data by reviewing institutional databases andthe patient’s medical charts ICK and MK obtained ... questionnaires and telephone interviews MK, PDS, and SS co-interpreted the data and critically reviewed the manuscript All authors approved the final version ofthe manuscript 19 20 21 22 Competing...
... clustering coefficient measures the extent of inter-connectivity among the neighbors ofthe node It is defined as the ratio ofthe number of existing connections among the node i’s neighbors and all their ... apply the MM-LDDMM registration into the average template generation in Chapter andinthe resting state functional MRI analysis in Chapter and Finally, in Chapter 8, we list our conclusions and ... important non-invasive technique for diagnosing and treating medical conditions ofthe brain, Magnetic resonance imaging (MRI) can help physicians evaluate both the structure and function ofthe brain...
... the production of amplified products during each cycle ofthe PCR reaction It is one ofthe technologies ofthe genomic age and has become the method of choice for detection of gene expression ... ascertain the exact position of each atom inthe asymmetric unit ofthe crystal Calculation of electron density is dependent on two main factors; the amplitude andthe phase ofthe diffracted beam These ... replication ofthe viral genome, assembly ofthe virus particle and inhibition of certain host cell functions These proteins are therefore potential candidates for drug design andthe development of vaccines...
... right The four functional domains oftheproteins are shown: the signal sequence, the passenger domain, the linker region, andthe β-domain The autotransporter polyproteins are synthesized and ... In T5SS the pre-protein consists ofthefollowing components: signal sequence, the passenger domain, linker region and β-strand The signal sequence which is present at the N-terminus guides the ... involved inthe development of diseases such as diarrhea and hemolytic-uremic syndrome after colonizing inthe large intestine, watery diarrhea after colonizing inthe small intestine and urinary...
... because ofthe complications they had developed during their hospitalization: (1) Diagnosis ofthe medical complication/s (2) Date of medical complication/s The information onthefollowing variables ... 79 Urinary incontinence: Post stroke urinary incontinence is more commonly seen inthe aged stroke patients than the younger ones.87,88 Stroke patients with urinary incontinence have more institutionalization ... al 44 in their prospective study at and 15 months on 486 patients found that worsening in cognition and worsening of depression between and 15 months follow up had an independent effect on the...
... theeffectsof aging onthe N2b component and, thus, upon selective information processing as a whole In one oddball detection study involving theeffectsof color deviation on N2b elicitation ... sporadically interspersed by a stimulus which deviated from the standard inthe intensity ofthe first component andthe glide direction ofthe second constituent The number of distinct MMN components ... effect onthe latency and amplitude ofthe evoked MMN, the elicited N2b was found to continuously increase in latency and decrease in amplitude with increasing age These findings suggest the constancy...
... Using this index, the proportion "feeling healthy" was 43% which is low when compared with similar observations made in other countries inthe region: 56% in Myanmar, 84% in Indonesia and 62% in ... within the year preceding the survey, information on problems related to mastication and to mobility Simple clinical examinations were carried out to identify problems with vision and hearing ... as an "informant" At each interview where an informant was present, assessment ofthe informant ofthe health status ofthe elderly person was obtained Results The non-response rate for the study...
... removed from the entire coding sequence ofthe MCPIP gene The coding sequence lacking the PIN domain was obtained in two steps First, the fragment of 423 bp situated in front ofthe PIN domain was ... degradation of IL-1b transcript independently ofthe presence of AREs (Fig 6B, lanes and 5) It is possible that inthe region located before the AREs, consisting of 343 bp ofthe 3¢ UTR ofthe IL-1b ... result in mRNA degradation or its conversion into translationally active forms by the action of strong stimulants, such as endotoxin (LPS) One ofthe regulatory sequences involved in determining...