... Copyright Computers and Structures, Inc., 1978-1997.The CSI Logo is a registered trademark of Computers and Structures, Inc.SAP2000 is a registered trademark of Computers and Structures, Inc.Windows ... the frame elements and joints.ã Click and hold the left mouse button.ã While holding, move the pointer below and to the right of the frame elements and joints.A “rubber-band” window will show ... Computers and Structures, Inc.Berkeley, California, USAIssue Date: June 1998Revision Number : 0Revision Date: N/ASAP2000IntegratedFinite Elements Analysis and Design of Structures SAP2000...
... improvement of ship and offshore installation safety standards, design standards and management. Accidents are the driving force of better safety and environmental protection standards. Ultimate ... safety culture and development of ship and offshore installations design standards. 2 ACCIDENT AND CASUALTIES - THE DRIVING FORCE TO IMPROVE SAFETY AND ENVIRONMENT PROTECTION STANDARDS In ... opposite that the inflation and interest rates are relatively low worldwide, government deficits significantly reduced, and the environment of domestic demand and external sale quite relaxed....
... extremities were carried out, using standard procedures. These included: semi tandem stand, full tandem stand, rising from chair without using arms and shoulder external rotation (full). ... illness. Best performance was in "standing from chair without using arms" (84%), semi tandem stand was completed by 78% and full tandem stand by 72%. The poorest performance was ... (IADL)" and included ability to travel outside, go shopping, prepare own meals and handle money. A limited number of tests of physical performance for assessing the functioning of upper and...
... HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine.Table 2. Km and Vmaxvalues for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 filter paper assay and ... Environment, AgriculturalSciences and Spatial Planning (to L.W. and S.E.), theSwedish Research Council (to H.E. and P.N.) and the Swedish Cancer Foundation (to H.E. and P.N.).The Structural Genomics ... S-(dimethylarsenic)cysteine. Libraryfiles and coordinates for GMP and S -(dimethylarsenic)cys-teine were obtained from the HIC-UP ligand database [36] and generated at the prodrg server [37]....
... biochemical and biophysical properties of WhiB2, WhiB5, WhiB6 and WhiB7 of M. tuberculosis and also compare the prop-erties of all seven WhiB proteins. We show that, simi-lar to WhiB3 and WhiB4, ... refolding were 98% pure and werebrownish (Figs S1 and S2).The presence of four conserved cysteines and thebrownish appearance of purified WhiB1, WhiB2,WhiB5, WhiB6 and WhiB7 indicated the ... structural andfunctional divergence amongseven WhiB proteins of Mycobacterium tuberculosisH37RvMd. Suhail Alam, Saurabh K. Garg* and Pushpa AgrawalInstitute of Microbial Technology, CSIR, Chandigarh,...
... coordinates and structure factors of thecrystal structures described in this studyhave been submitted to the Protein DataBank, and the structures have beenassigned the accession codes 2v4n and 2v4o ... arerepresented by pink, cyan, blue and yellow, respectively. The tetra-meric interactions are between the subunits A and C, and B and D,by way of the b hairpins and the loop, which connects b2tob3(boxed).Structure ... Science and Technology (DST) and the Depart-ment of Biotechnology (DBT) of the Government ofIndia. MRN and HSS thank DST and DBT for finan-cial support. AP acknowledges the Council for Scientificand...
... 2,3 and 4, lower band). The levels of mutant and wild-type MCPIP mRNAs were higher when thesevectors were co-transfected with a vector overexpress-ing IL-1b (Fig. 5, lanes 3 and 4, upper bands). ... (LSHM-CT-2006-036903,MTKD-CT-2006-042586 and COST Action BM0602) and by the Polish Ministry of Scientific Research and Information Technology (2 P05A01127, 63⁄ 6PR-UE ⁄ 2007 ⁄ 7 and 339 ⁄ 6PR-UE ⁄ 2007 ⁄ 7). ... intermediate levels inheart and placenta, and the lowest levels in spleen, kid-ney, liver and lung. The transcript was not detectablein brain, thymus, muscles, small intestine and colon(Fig. 3A)....
... performedwith rabbit preimmune and post-third inoculation sera against aM. tuberculosis sonicate (lanes 1, 2 and 5) and a membrane frac-tion (lanes 3 and 4). Lanes 1 and 3, absence of recognition ... addition of cysteine at theamino and carboxy termini and mixed with Freund’sIncomplete Adjuvant (Sigma)] on days 0, 20 and 40. Finalbleeding was carried out on day 60 and sera were collectedin accordance ... (Fig. 5A). Scat-chard and Hill analyses were then performed and dis-sociation constants (Kd) were calculated for Rv2560HABP 11024 for U937 and A549 cell lines (Kdof 380 and 800 nm, respectively)....
... D and E + F), (b) a few hydrogenbonds, and a hydrophobic interaction between A + C,B + E and D + F, and (c) electrostatic forces in thecentral channel of the hexamer between B, C and F, and ... water molecule fix-ing the three lysines from the B, C and F subunits, and there is no direct interaction between subunits A and F, B and D, or C and E.A phosphate ion was found in the donor site ... The mutant enzymes, F133N and F133A, were expressed, purified and characterized.With 1 mm UMP and ATP as substrates, the activitiesof F133N and F133A were only 50 and 20% of thatof the wild-type...
... and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of1 nm. Thermal stability of Rep245 was measured by incu-bating the protein at 60, 70 and 80 °C for 30 min and ... follows: Na-acetate (pH 5.0, 5.4 and 5.8),Tris ⁄ HCl (pH 6.5, 7.0, 7.5 and 8.0) and glycine ⁄ NaOH(pH 8.6, 9.0 and 9.6). Reactions were carried out for30 min at 65 °C and the products resolved by ... measured at 65 °C) were asfollows: Na-acetate (pH 5.0, 5.4 and 5.8), Tris ⁄ HCl (pH 6.5, 7.0, 7.5 and 8.0) and glycine ⁄ NaOH (pH 8.6, 9.0 and 9.6). (B) Dependence ofRep245 polymerase activity on...
... numbers AJ880690 and AJ880691 for cDNAs,Q53I65 and Q53I64 for amino-acid sequences) werealigned and compared with other globins in the mul-tiple sequence alignment (Fig. 1 and Table 1). Thesequence ... diversity in sequence,quaternary structure and functions such as binding and transport of oxygen and hydrogen sulfide, and activity of superoxide dismutase and mono-oxygenase[8].Annelid noncirculating ... reversiblybind oxygen and other gaseous ligands, and are wide-spread in the three major kingdoms of life [1,2].Despite the great diversity of their amino-acidsequences, the basic functional unit...
... glucose and hemin chloride (13 lgặmL)1), and further incubated at30 C for 24 h. Then, the cells were collected and suspen-ded in 25 mL of 40% potassium hydroxide and 25 mL ofmethanol and refluxed ... soyasapogenol B, and soyasapogenol E, oleanene sapogenols from soybean, structures of soyasaponin I, soyasaponin II, and soyasa-ponin III. Chem Pharm Bull 30, 2294–2297.35 Ireland PA & Dziedzic ... aglycone structures are restrictedto two, soyasapogenols A and B (Fig. 2). Soyasapoge-nol A has four hydroxyl groups at C-3, C-21, C-22, and C-24, whereas soyasapogenol B has three at C-3,C-22, and...
... disulfidebridges in PDGF-C to consist of Cys250 and 294,Cys280 and 335, and Cys287 and 337, and the inter-monomeric bonds to consist of Cys274 and 286 [57].At present, analysis of PDGF ⁄ VEGF ... PDGF-A and -B can form both homo- and heterodimers (PDGF-AA, -AB, -BB), PDGF-C and -Dexist only as homodimers (PDGF-CC and -DD). Thefull-length PDGF-C and -D monomers are 54–55 kDa and 49–56 ... PDGF-c (exons 5 and 6) and PDGF-d(exons 6 and 7) encoding the cystine knot motifsresemble the corresponding exons in the PDGF-a and -b (exons 4 and 5) genes. Both in PDGF-a and -b, exon6 encodes...
... Fish and molluscan metallothioneinsA structural andfunctional comparisonLaura Vergani1, Myriam Grattarola1, Cristina Borghi2, Francesco Dondero3 and Aldo Viarengo3,41 ... FEBS right-handed b domain binds three bivalent metal ions.The C-terminal a domain is left-handed and binds fourbivalent ions. Zinc is preferentially located in the b do-main and cadmium in ... snail and oyster) haveso far been elucidated [11–17]. As in mammals and fish[18], echinoderm MTs contain two globular domainsbinding four and three bivalent ions [19]. On the otherhand, in...
... PQE-30-AK, were washed, folded and purified as described in Materials and methods. Purified enzyme aliquots were maintained at 4 °C(m), 13 °C(j) and 26.5 °C(d) and AK activity was measured at ... in Asian and Mediterranean countries [15]. This paper describes thecloning andfunctional characterization of an AK gene froma genomic library of L. donovani as well as its expression and molecular ... characterization.Materials and methodsMaterialsPlasmids pGEM-3Zf(+) and pQE30 were from Promega and QIAGEN, respectively. [32P]dCTP[aP] (3000 Ciặmmol)1) was from DuPont-NEN. Restriction enzymes and Taq DNA...