colorful and functional shell prompt using ps1
Label free electrochemical DNA and protein detection using ruthenium complexes and functional polyethylenedioxythiophenes
... Results and Discussions 102 6.3.1 Synthesis and Characterization of Functional PEDOT Thin Films 102 6.3.2 Biocompatibility of Functional PEDOT Thin Films 106 6.3.3 Adhesive and Non-adhesive ... Particularly, biotin-functionalized PEDOT surface and peptide-functionalized PEDOT surface were achieved through direct polymerization from mixed monomer solution and facile post-polymerization functionalization ... 6-16: Controlled cell attachment (NIH3T3) on (a) RGD-functionalized, (b) carboxylic acid-functionalized, and (c) RDG-functionalized PEDOT surfaces Functional group density was controlled at 10% while...
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Báo cáo y học: "Comparison of osteogenic potentials of human rat BMP4 and BMP6 gene therapy using [E1-] and [E1-,E2b-] adenoviral vectors"
... (Lanes 1, and 3), BstXI (Lanes 4, 5, and 6), and BglII plus EcoRV (Lanes 7, 8, and 9) M, 1-kb DNA ladder Lanes 1, 4, and 7, 293A cells; Lanes 2, 5, and 8, ADrBMP6 DNA; Lanes 3, 6, and 9, ADhBMP6 ... thigh and 1.4 × 1011 particles in the other thigh) On Day 35, the rats were euthanized and scanned using CT Axial CT images (1-mm collimation and 1mm table increment) were obtained using the standard ... BMP4 and BMP6 cDNAs were amplified, cloned, sequenced, and identified Recombinant adenoviruses encoding rat BMP4 and BMP6 were constructed and compared with ADhBMP4 and ADhBMP6 in the in vitro and...
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Natural botanical products have a long history in the world and are featured in using a complex
... tumorigenesis including cell proliferation and apoptosis [7], and also angiogenesis [8] were assessed in gastric tumors after drug treatment Materials and methods Chemicals and reagents CKBM-A01 (Batch no.: ... were measured every days using a caliper, and the tumor area was calculated according to the formula: tumor volume (mm3) = d2 x D/2, where d and D were the shortest and the longest diameter respectively ... proliferation and promotion of apoptosis in this model Dietary supplement containing Schisandra chinensis has been shown to reduce prostate cancer cell growth and induce apoptosis by inhibiting androgen...
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Optimal cost and allocation for UPFC using HRGAPSO to improve power system security and loadability
... rand1 * ( pi (τ ) − xi (τ )) + c * rand * ( p g (τ ) − xi (τ )) (30) where c1 and c are acceleration constants and rand and rand are uniformly distributed random r numbers in [0, 1] The term vi ... where PGi and QGi : generator real and reactive power at i-th bus, respectively; PDi and QDi : load real and reactive power at i-th bus, respectively; Gij and Bij : transfer conductance and susceptance ... dispatching and the stability of EPS, wind energy and intelligent techniques applications in EPS He is author and co-author of about 18 papers in international journals and national and international...
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Heat transfer and friction factor characteristics using continuous M shape ribs turbulators at different orientation on absorber plate solar air heater
... The entry and exit section length were 250 mm and 125 mm respectively ASHRAE standard 97-77 [32] recommends the minimum entry and exit length of 5√ (WH) and 2.5√ (WH), i.e 236.6 mm and1 18.3 mm ... section and outlet section respectively The rectangular duct is1375 mm × 287 mm × 197 mm and fabricated with the red marandi wood and 19 mm thick plywood panel The test section is of 1000 mm long and ... respectively Verma and Prasad [19] investigated the effect of transverse wire roughness on heat and flow characteristics of two roughened collectors and one plane surface collector Saini and Verma [20]...
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Tài liệu Monitoring and Diagnosing Manufacturing Processes Using Fuzzy Set Theory pptx
... functions and conditional mass functions using Equations 14.10 and 14.14 Finally, the fuzzy transition probability and limiting fuzzy transition probability can be calculated using Equations 14.42 and ... function defined over {t, f, u} (t represents true, f represents false, and u stands for uncertain) Let mg = {Li: li} and mg′ = {Mi: mi} and form a matrix {( } ) ( M = T Li / M j : li m j , where T Li ... [0.3, 0.7] 14.3 Monitoring and Diagnosing Manufacturing Processes Using Fuzzy Sets 14.3.1 Using Fuzzy Systems to Describe the State of a Manufacturing Process For monitoring and diagnosing manufacturing...
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Tài liệu Serum cytokine profiles in healthy young and elderly population assessed using multiplexed bead-based immunoassays doc
... interpretation and in the writing HOK and HSK performed the analysis of the data and drafted the manuscript JCY and ECS participated in the design of the study and performed the statistical analysis and ... (Tables and and Figure 1) TGF-a binds to the EGF receptor with a high affinity [21] and is indispensable for the proper development of many tissues and organs, wound healing, bone resorption, and ... aging (Tables and and Figure 1) The CD40/CD40L system belongs to the tumor necrosis factor superfamily and is a key pathway that links inflammation and atherothrombosis [17] CD40 and CD40L are...
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Tài liệu Physical health and functional ability of an elderly, population in Sri, Lanka doc
... and lower extremities were carried out, using standard procedures These included: semi tandem stand, full tandem stand, rising from chair without using arms and shoulder external rotation (full) ... injury or an illness Best performance was in "standing from chair without using arms" (84%), semi tandem stand was completed by 78% and full tandem stand by 72% The poorest performance was in shoulder ... healthy Yes No (n - 518) (n = 664) % % p value (using x ) Physical performance test Semi tandem stand Full tandem stand Able to sit up without using arms Shoulder external rotation 85 81 87 78...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... PRTFDC1 (A) monomer and (B) tetramer generated using symmetry-related molecules Interactions with GMP (C) GMP, phosphate and calcium ions are shown as sticks and spheres colored pink and black, respectively ... Km and Vmax values for Hx and G in the presence of mM PRPP, determined using the DE-81 filter paper assay and tritium-labeled substrates Experiments have been repeated three to four times and ... Library files and coordinates for GMP and S-(dimethylarsenic)cysteine were obtained from the HIC-UP ligand database [36] and generated at the prodrg server [37] All figures were condtructed using pymol...
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Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf
... (a helix signature), 218 nm (b strand signature), 202–204 nm (random coil signature) In WhiB5 and WhiB6, the structure was dominated by a helices and b strands and the proportion of these structural ... refolding were 98% pure and were brownish (Figs S1 and S2) The presence of four conserved cysteines and the brownish appearance of purified WhiB1, WhiB2, WhiB5, WhiB6 and WhiB7 indicated the presence ... WhiB6 and WhiB7 the loss was 8–10% After 48 h of air exposure, losses were as follows: 80% in WhiB1, 75% in WhiB2 and WhiB5, 65% in WhiB3, 60% in WhiB4, and 35–40% in WhiB6 and WhiB7...
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Tài liệu Báo cáo khoa học: Structural and functional studies on a mesophilic stationary phase survival protein (Sur E) from Salmonella typhimurium ppt
... between R81 and P82 and between G90 and I91 A similar cis peptide between G93 and V94 is found in Tm SurE also Overall structural features of the protein St SurE is an aba sandwich protein and adopts ... archaic and thermophilic homologues The St SurE monomer consists of 13 b-strands, six a-helices and three 310-helices The core of the protein is made up of a nine-stranded b-sheet flanked by a1, a5 and ... are represented by pink, cyan, blue and yellow, respectively The tetrameric interactions are between the subunits A and C, and B and D, by way of the b hairpins and the loop, which connects b2 to...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
... lanes 2,3 and 4, lower band) The levels of mutant and wild-type MCPIP mRNAs were higher when these vectors were co-transfected with a vector overexpressing IL-1b (Fig 5, lanes and 4, upper bands) ... intermediate levels in heart and placenta, and the lowest levels in spleen, kidney, liver and lung The transcript was not detectable in brain, thymus, muscles, small intestine and colon (Fig 3A) The ... R263, V139 and N144 Determination of the MCPIP domain architecture was completed using the DISOPRED [9] and SPRITZ servers [10], which consistently indicated that regions 1-50, 90-130 and 290-540...
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Tài liệu Báo cáo khoa học: The Mycobacterium tuberculosis membrane protein Rv2560 ) biochemical and functional studies pdf
... performed with rabbit preimmune and post-third inoculation sera against a M tuberculosis sonicate (lanes 1, and 5) and a membrane fraction (lanes and 4) Lanes and 3, absence of recognition of ... nm) (Fig 5A) Scatchard and Hill analyses were then performed and dissociation constants (Kd) were calculated for Rv2560 HABP 11024 for U937 and A549 cell lines (Kd of 380 and 800 nm, respectively) ... for U937 and 1.2 for A549 cells, suggesting positive ligand–receptor cooperativity The numbers of binding sites per cell calculated for peptide 11024 were 200 000 and 000 000 for U937 and A549...
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Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt
... D and E + F), (b) a few hydrogen bonds, and a hydrophobic interaction between A + C, B + E and D + F, and (c) electrostatic forces in the central channel of the hexamer between B, C and F, and ... water molecule fixing the three lysines from the B, C and F subunits, and there is no direct interaction between subunits A and F, B and D, or C and E A phosphate ion was found in the donor site ... Ala The mutant enzymes, F133N and F133A, were expressed, purified and characterized With mm UMP and ATP as substrates, the activities of F133N and F133A were only 50 and 20% of that of the wild-type...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... prim–pol domain S Prato et al Zn-binding motif and the two disulfide bonds respectively connecting the a4-helix to the b4 strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in ... follows: Na-acetate (pH 5.0, 5.4 and 5.8), Tris ⁄ HCl (pH 6.5, 7.0, 7.5 and 8.0) and glycine ⁄ NaOH (pH 8.6, 9.0 and 9.6) Reactions were carried out for 30 at 65 °C and the products resolved by denaturing ... known to serve as ligands for divalent cations and as tags revealing the presence of DNA polymerases in the active site [18–20] In this study, we report on the structural and functional characterization...
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Tài liệu Báo cáo khoa học: Globin gene family evolution and functional diversification in annelids ppt
... muscle/input_muscle.py) and adjusted manually Molecular phylogeny For the two sets of aligned globins (annelids on one hand and annelids, molluscs and arthropods on the other), Bayesian analysis was carried out using ... typical molecular features: Lys-A9, Trp-B10 and also a deletion of three residues between the A and B helices, and a deletion of one residue between the F and G helices Moreover, the two residues ... Asp-A4, Trp-A16, Phe-B10 and LeuB13 Furthermore, it shows an insertion of three residues between the A and B helices, an insertion of one residue between the F and G helices, and a three-residue motif...
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Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx
... glucose and hemin chloride (13 lgÆmL)1), and further incubated at 30 °C for 24 h Then, the cells were collected and suspended in 25 mL of 40% potassium hydroxide and 25 mL of methanol and refluxed ... °C for min, and 72 °C for min, and a final extension reaction for 10 at 72 °C) using Ex Taq DNA polymerase (Takara Bio Inc, Shiga, Japan) with template (the cDNA pool described above) and primers ... electrophoresis and ligated into SalI and ClaI-digested and dephosphoryrated pESC-PSY The resulting plasmid was designated pESC-PSY-CYP93E1 S cerevisiae strain GIL77 was transformed with this plasmid using...
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Tài liệu Báo cáo khoa học: Structural and functional specificities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx
... chromosomes; PDGF-a and -b on chromosomes and 22 [17,18], and PDGF-c and -d on chromosomes and 11 [19], respectively The genomic organization of the pdgf genes is quite similar, although PDGF-c and -d genes ... exons in PDGF-c (exons and 6) and PDGF-d (exons and 7) encoding the cystine knot motifs resemble the corresponding exons in the PDGF-a and -b (exons and 5) genes Both in PDGF-a and -b, exon encodes ... While PDGF-A and -B can form both homo- and heterodimers (PDGF-AA, -AB, -BB), PDGF-C and -D exist only as homodimers (PDGF-CC and -DD) The full-length PDGF-C and -D monomers are 54–55 kDa and 49–56...
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Tài liệu Báo cáo khoa học: Fish and molluscan metallothioneins A structural and functional comparison ppt
... concentrations: 40 and 80 mM (lanes and 3) for h MTs were also treated for the same period with a reducing agent (dithiothreitol) at 40 and 80 mM (lanes and 5) To reduce aggregation, MT samples were handled ... of cadmium bound per molecule of MT was determined using as a standard curve constructed using a standard solution of cadmium chloride Both fish and mussel recombinant MTs contain equivalents of ... Vergani et al right-handed b domain binds three bivalent metal ions The C-terminal a domain is left-handed and binds four bivalent ions Zinc is preferentially located in the b domain and cadmium in...
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Tài liệu Báo cáo khoa học: "Extracting Comparative Entities and Predicates from Texts Using Comparative Type Classification" pptx
... probability of CE-candidates using MEM and SVM Both MEM and SVM showed outstanding performance; there was no significant difference between the two machine learning methods (SVM and MEM) Hence, ... as a candidate for SE/OE, and each of adjective (or verb) words as a candidate for PR However, this candidate detection has serious problems as follows: 4) There are many actual SEs, OEs, and PRs ... (Task 2) Entities and This section explains how to extract comparative entities and predicates Our strategy is to first detect Comparative Element candidates (CEcandidates), and then choose the...
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