collection separation and storage of mouse and human plasma samples
Báo cáo y học: " Molecular characterization of two distinct monopartite begomoviruses infecting tomato in india" pps
Ngày tải lên :
12/08/2014, 02:20
... analysis of the coat protein and CR of the newly identified ToLCV isolates (A) Nucleotide sequence of the 3’ region of the CP gene of the two new ToLCV isolates from India (CTM, K3/K5) The sequence of ... (Figure 2C) The degree of relationship of the nucleotide sequence of Intergenic Region (IR) and the amino acid sequences of the proteins expressed by the viral genomes of the three ToLCV isolates ... (TAATAA), the first and the second nucleotides of which are the second and first nucleotides respectively of the stop codon of the complementary C3 gene [13], as observed in the case of ToLCV-CTS,...
- 10
- 360
- 0
Báo cáo khoa học: "Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand" potx
Ngày tải lên :
07/08/2014, 23:22
... identities of 97∼ 98% and amino acid sequence identities of 96∼98% with Chinese IBVs (strain A2, SH and QXIBV) Discussion One of the major problems of IBV is the frequent emergence of new variants ... typing of IBV Genotyping of IBV on the basis of the S1 gene sequence, particularly the HVR region of the S1 gene, is the most Nucleotide and amino acid sequence comparison The S1 gene of the ... for amplification of a segment of 878-bp of the S1 gene coding region using a primer combination of FOR1 and RE3 The amplification reaction was carried out with one-step RT-PCR and the reaction...
- 5
- 537
- 0
Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
Ngày tải lên :
18/02/2014, 08:20
... positions of the Ca of Ala156 in HYDJs and the Ca of Met156 in HYDBp located in the SGL3, which leads to the increased size of the substrate binding pocket of HYDJs The side chain of Ala156 is ... performed using T-COFFEE [44] Docking simulation of substrate on the active site of HYDJs The substrate binding pockets of HYDJs and the mode of interaction between substrate and enzyme were analyzed ... activity of HYDJs against the tested substrates (Table 3) B Fig Homology model of HYDJs (A) Docking of D-p-HPH to the HYDJs active site (B) The SGLs of HYDBp are shown as magenta lines and those of...
- 14
- 621
- 0
Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx
Ngày tải lên :
07/03/2014, 15:20
... fully active alpha isoform and other variants, we conducted a series of cotransfection experiments and compared ligand-induced accumulation of cAMP (Fig 3) Although the level of cAMP accumulated ... and pharmacology Biochem Biophys Res Commun 205, 1836–1842 Pisarchik, A & Slominski, A (2001) Alternative splicing of CRH-R1 receptors in human and mouse skin: identification of new variants and ... messages with internal deletions and unusual isoforms composed of soluble extracellular (ligand-binding) domains [18] As the skin shows polymorphism in CRFR1 expression and its functional diversity...
- 10
- 671
- 0
Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf
Ngày tải lên :
21/02/2014, 00:20
... reaction products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE As shown in Fig 4, 9-HODE is the main product of the hazelnut ... C6 and C9 aldehydes (and the corresponding alcohol) were detected in all the samples and, similarly to the pattern of LOX expression and activity, their levels were higher in stage I seed and ... cm, lm particle size) with a solvent system of hexane/propan-2-ol/acetic acid (100 : : 0.1, v/v/v) and a flow rate of mLÆmin)1 Authentic standards of 9- and 13-HODE were purchased from ICN HPLC analyses...
- 11
- 404
- 0
Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx
Ngày tải lên :
07/03/2014, 15:20
... of 3.0 corresponding to a specific activity of 13.5 UÆmg protein)1 Corresponding pH optima and specific activities for syringaldazine and 2,6-DMP were pH 5.6 and 3.4 UÆmg protein)1 and pH 4.6 and ... apparent Km value of 0.03 mM and a Vmax of 16.4ÆU mg protein)1 Corresponding values for syringaldazine and 2,6-DMP under optimal conditions were 0.01 mM and 4.9 UÆmg protein)1, and 0.57 mM and 5.6 UÆmg ... only one band was amplified from each of the different samples and Southern blot analysis using the probe prepared with the lac1 primers also revealed a single band Furthermore, all of these generated...
- 11
- 703
- 0
Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deficient families from Southern Italy docx
Ngày tải lên :
16/03/2014, 01:20
... to Erlandsen and Stevens [22] The details of the interactions displayed by residues in the neighborhood of Q301 were analyzed in the structure of the ternary complex of human PAH with BH4 and thienylalanine, ... that it is often difcult to predict the phenotype on the basis of a patients genotype, and further studies in different ethnic groups are still warranted Function and structure of PAH human variants ... family and a shorter fragment of 253 bp present only in the proband, as well as in his mother who bears the same mutation (Fig 3) Direct sequencing of both cDNA bands conrmed the skipping of the...
- 12
- 491
- 0
Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii ppt
Ngày tải lên :
23/03/2014, 13:20
... space group of the 1Q9H crystal, were found The best solutions had correlation coefficients of 56.1% and 55.3%, and R-factors of 40.0% and 40.9% for 1CEL and 1GPI, respectively Refinement of the models ... was 1.8°, the standard deviation of the hydrogen bond energies was 0.7 and overall G-factor, a measure of the normality of the structure, was 0.0 Fig SDS/PAGE and activity analysis of recombinant ... in position With the exception of four a-helices and two pairs of short b-strands, the rest of the protein consists almost entirely of loops connecting the b-strands The loops extending from the...
- 12
- 553
- 0
Báo cáo khoa học: Biochemical and molecular characterization of purified chicken pancreatic phospholipase A2 docx
Ngày tải lên :
30/03/2014, 01:20
... absence of NaTDC The maximal PLA2 specific activity of 400 UÆmg)1 was measured at pH 9.5 and at 37 °C in the presence of mm NaTDC and mm CaCl2 Pure ChPLA2 lost 80% of its activity after 20 of incubation ... Lys56; and aD, Ala90–Lys108); (e) two short strands 4550 of an antiparallel b-sheet (b-wing) (b2, Ser74–Ser78; and b3, Glu81–Asn85); and (f) a C-terminal loop containing a short b4-strand (His115–Asn117) ... 4547 A Karray et al A effect of bile salts on ChPLA2 activity, the rate of hydrolysis of PC by ChPLA2 in the presence of various concentrations of bile salts, at pH 9.5 and at 37 °C, was studied...
- 10
- 358
- 0
Báo cáo sinh học: " Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
Ngày tải lên :
19/06/2014, 08:20
... degradation during sampling and storage Combined with the lack of bulk offered by paper-based collection, the potential number of samples that can be collected within a given time and location is significantly ... consisted of 30 cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C ... is effective for sampling, storage and retrieval of viral pathogens from infected plant tissues growing under the greenhouse and field conditions Storage and transport of purified nucleic acids...
- 12
- 571
- 0
báo cáo hóa học:" Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
Ngày tải lên :
20/06/2014, 04:20
... degradation during sampling and storage Combined with the lack of bulk offered by paper-based collection, the potential number of samples that can be collected within a given time and location is significantly ... consisted of 30 cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C ... is effective for sampling, storage and retrieval of viral pathogens from infected plant tissues growing under the greenhouse and field conditions Storage and transport of purified nucleic acids...
- 12
- 387
- 0
Báo cáo sinh học: "Cytogenetic and molecular characterization of eight new reciprocal translocations in the pig species. Estimation of their incidence in French populations" pot
Ngày tải lên :
14/08/2014, 13:21
... performance of the sows sired by two of these boars only (cases and 6) The boar carrier of the 2/6 translocation sired 51 litters in two herds, with an average litter size of 8.30 The boar carrier of ... detection of rearrangements modifying very slightly the size and/ or the banding profiles of the chromosomes, as, for instance, in the case of the translocations rcp(15;17)(q24;q21) and rcp(7;8)(q24;p23) ... long light band (corresponding to the 17q21 → 17qter fragment) at the terminal extremity of one rearranged chromosome 15, and the concomitant lack of the very characteristic 15q25 band (15q24...
- 18
- 381
- 0
IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE
Ngày tải lên :
24/08/2014, 09:51
... you for believing in me and letting me pursue my dreams The person I am today is because of you To my dear uncle and my aunt Khem Kandel and Laxmi Kandel: your support and friendship has been ... structure of ALDH3A1 with CB7 .72 Crystal structure of ALDH3A1 with CB25 81 G Expression and Activity of ALDH3A1 and ALDH1A1 in Cancer Cell lines .86 H Sensitization of tumor cells ... experiments of CB7 with benzaldehyde and NADP+ 56 Figure 16: Competition experiments of CB29 with benzaldehyde and NADP+ .57 Figure 17: Competition experiments of CB25 with benzaldehyde and NADP+...
- 179
- 351
- 0
Expressed sequence tags analysis of major allergens producing dust mites and molecular characterization of their allergens
Ngày tải lên :
12/09/2015, 11:08
... Profile of allergen collections of D farinae and B tropicalis 114 Table 13: Percentage of amino acid sequence homology between the mites FABP homologues 167 Table 14: IgE reactivity profiles of ... temperature of 23-30o C and 80-90% relative humidity The house dust mites primarily feed on skin scales and dander while the storage mites feed on plants and micro-organisms (Ho and Nadchatram, 1984; Andersen ... distribution of common dust mites 16 Figure 4: An outline of the generation of EST collection 44 Figure 5: Redundancy rate of ESTs generated for D farinae and B tropicalis 85 Figure 6: Determination of...
- 312
- 4K
- 0
Isolation and molecular characterization of genes associated with shoot regeneration of mustard (brassica juncea) in vitro
Ngày tải lên :
17/09/2015, 17:19
... Ethylene and PAs are ubiquitous in plants and both are involved in the regulation of various physiological processes during plant growth and development (Yang and Hoffman, 1984; Evans and Malmberg, ... et al., 2003) and Arabidopsis thaliana (Ikeda-Iwai et al., 2003), and androgenesis in rye (Immonen and Anttila, 1999) and triticale (Immonen and Robinson, 2000) An accumulation of H2O2 in culture ... range of plant species These tissue culture systems are powerful tools for the study of fundamental aspects of plant biology leading to a better understanding of various processes and control of...
- 233
- 526
- 0
Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
Ngày tải lên :
07/03/2014, 21:20
... 2B that VBARP-L and VBARP-S are coded by these specific exons VBARP-L and VBARP-S code for precursor proteins of 627 and 435 amino acids and with calculated peptide masses of 69 and 49 kDa, respectively ... distribution of VBARP is independent of the cycling stage of the cells Identification of biological function(s) of VBARP using a siRNA assay To examine the role of endogenous VBARP in the regulation of ... VBARP-L consisted of a 576-bp fragment absent from the shorter VBARP-S and contained a 114-bp UTR (Fig 2A) Intron and exon analysis of VBARP revealed that VBARP-L and VBARP-S contain 11 and exons, respectively...
- 12
- 561
- 0
Báo cáo khoa học: "Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods" pdf
Ngày tải lên :
07/08/2014, 17:22
... count analysis of the corresponding milk samples revealed the subclinical status of mastitis for 126 of the 131 selected samples However, five of the samples exhibited a clinical status of mastitis ... and Wessman, G E Biochemical and serological characteristics of Lancefield groups E, P, and U streptococci and Streptococcus uberis Cornell Vet 1972, 62, 540-562 55 Schuman, R D., Wood, R L and ... streptococci investigated 94 and of the strains fermented inulin and raffinose, respectively Additionally, 130 of the investigated strains, and the S uberis reference strains NCDO 2038 and NCDO 2086 showed...
- 11
- 508
- 0
Identification and characterization of novel anticoagulants from bungarus fasciatus venom
Ngày tải lên :
09/09/2015, 11:08
... substrate in plasma [93] Human FXI is a disulfide-linked homodimer presenting in human plasma at a concentration of to µg/mL (approximately 30 nM) [94] Plasma FXI circulates as a zymogen in human plasma ... Anticoagulant activity of pooled fractions Chapter Three Figure 3.1: Purification of BF-AC1 and BF-AC2 from the venom of B fasciatus Figure 3.2: ESI-MS profile of BF-AC1 (A) and BF-AC2 (B) Figure ... characterization of BF-AC1 and BF-AC2 XI Figure 3.4: N-terminal sequence alignment of Chain A and Chain B of BF-AC1 and BF-AC2 with protein sequences in the database Figure 3.5: Anticoagulant activity of BF-AC1...
- 180
- 434
- 0
Identification and characterization of novel proteins from a rare australian elapid snake drysdalia coronoides
Ngày tải lên :
11/09/2015, 10:02
... 41 Materials and Methods 42 iii Reagents and kits; Collection of venom and venom gland; RNA isolation and cDNA synthesis; Cloning of ds cDNA; Isolation of plasmids and verification of clones; cDNA ... about 2,930 species of snakes distributed on every continent except Antarctica, islands of Ireland, Iceland and New Zealand [5, 6] They have successfully colonized various habitats and feed on small ... gene into pET-32a and pET-M; Transformation of cloning and expression host strains; Expression of protein; Purification of protein; Mass determination; Refolding of protein Results and discussion...
- 268
- 406
- 0
Identification and characterization of novel group 5 and group 21 allergens from dust mite and ige binding epitope mapping of blo t 5
Ngày tải lên :
14/09/2015, 12:44
... diagnosis and therapy 28 1.8 Objectives of this study 30 32 Chapter 2: Materials and Methods 2.1 Dust mite samples 2.2 32 Cloning of Group and Group 21 allergens and site-directed 32 mutagenesis of ... Effect of thermal treatment on IgE-binding activities of Blo t 92 and Blo t 21 4.2.2 Effect of thermal treatment on Blo t and Blo t 21 folding 94 4.2.3 Resistance of IgE-binding of Blo t and Blo ... organization of the gene encoding Der f 21 and 111 Der f 5.2.3 Southern blot of Der f 21 and Der f 116 5.2.4 Secondary structures of Der f 21 and Der f 118 5.2.5 IgE-binding of Der f 21 and Der f...
- 259
- 432
- 0